{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:09:40Z","timestamp":1787314180253,"version":"build-2736575974"},"reference-count":64,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T00:00:00Z","timestamp":1594598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["#2015\/06766-0; #2017\/02739-4"],"award-info":[{"award-number":["#2015\/06766-0; #2017\/02739-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["#302524-2016-9, #301771\/2019-7"],"award-info":[{"award-number":["#302524-2016-9, #301771\/2019-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background: Cancer-cachexia induces a variety of metabolic disorders, including skeletal muscle imbalance. Alternative therapy, as nutritional supplementation with leucine, shows a modulatory effect over tumour damage in vivo and in vitro. Method: Adult rats distributed into Control (C), Walker tumour-bearing (W), control fed a leucine-rich diet (L), and tumour-bearing fed a leucine-rich diet (WL) groups had the gastrocnemius muscle metabolomic and proteomic assays performed in parallel to in vitro assays. Results: W group presented an affected muscle metabolomic and proteomic profile mainly related to energy generation and carbohydrates catabolic processes, but leucine-supplemented group (WL) recovered the energy production. In vitro assay showed that cell proliferation, mitochondria number and oxygen consumption were higher under leucine effect than the tumour influence. Muscle proteomics results showed that the main affected cell component was mitochondria, leading to an impacted energy generation, including impairment in proteins of the tricarboxylic cycle and carbohydrates catabolic processes, which were modulated and improved by leucine treatment. Conclusion: In summary, we showed a beneficial effect of leucine upon mitochondria, providing information about the muscle glycolytic pathways used by this amino acid, where it can be associated with the preservation of morphometric parameters and consequent protection against the effects of cachexia.<\/jats:p>","DOI":"10.3390\/cancers12071880","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T11:03:23Z","timestamp":1594724603000},"page":"1880","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Leucine-Rich Diet Modulates the Metabolomic and Proteomic Profile of Skeletal Muscle during Cancer Cachexia"],"prefix":"10.3390","volume":"12","author":[{"given":"Bread","family":"Cruz","sequence":"first","affiliation":[{"name":"Department Structural and Functional Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department Structural and Functional Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5913-102X","authenticated-orcid":false,"given":"Lais Rosa","family":"Viana","sequence":"additional","affiliation":[{"name":"Department Structural and Functional Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leisa","family":"Lopes-Aguiar","sequence":"additional","affiliation":[{"name":"Department Structural and Functional Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8722-8512","authenticated-orcid":false,"given":"Rafael","family":"Canevarolo","sequence":"additional","affiliation":[{"name":"Department Cancer Physiology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maiara Caroline","family":"Colombera","sequence":"additional","affiliation":[{"name":"Department Structural and Functional Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael Rossi","family":"Valentim","sequence":"additional","affiliation":[{"name":"Department Structural and Functional Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Garcia-F\u00f3ssa","sequence":"additional","affiliation":[{"name":"Nano-Cell Interactions Lab, Department Biochemistry and Tissue Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4843-402X","authenticated-orcid":false,"given":"Lizandra Maia","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Tissue Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1534-305X","authenticated-orcid":false,"given":"Bianca Gazieri","family":"Castelucci","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Tissue Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3149-021X","authenticated-orcid":false,"given":"S\u00edlvio Roberto","family":"Consonni","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Tissue Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3595-5846","authenticated-orcid":false,"given":"Daniel","family":"Martins-de-Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, 13083-862 S\u00e3o Paulo, Brazil"},{"name":"Experimental Medicine Research Cluster (EMRC), University of Campinas, 13083-862 S\u00e3o Paulo, Brazil"},{"name":"D\u2019Or Institute for Research and Education (IDOR), 04501-000 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0812-1491","authenticated-orcid":false,"given":"Marcelo Bispo","family":"de Jesus","sequence":"additional","affiliation":[{"name":"Nano-Cell Interactions Lab, Department Biochemistry and Tissue Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5491-900X","authenticated-orcid":false,"given":"Steven Thomas","family":"Russell","sequence":"additional","affiliation":[{"name":"School of Bioscience, College of Health and Life Sciences, Aston University, Birmingham B4 7ET, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1713-756X","authenticated-orcid":false,"given":"Maria Cristina Cintra","family":"Gomes-Mardondes","sequence":"additional","affiliation":[{"name":"Department Structural and Functional Biology, Institute of Biology, UNICAMP, Campinas, 13083-862 S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"212265","DOI":"10.7573\/dic.212265","article-title":"One-year prevalence, comorbidities and cost of cachexia-related inpatient admissions in the USA","volume":"3","author":"Arthur","year":"2014","journal-title":"Drugs Context"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1038\/nrclinonc.2012.209","article-title":"Understanding the mechanisms and treatment options in cancer cachexia","volume":"10","author":"Fearon","year":"2013","journal-title":"Nat. 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