{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T07:47:53Z","timestamp":1770623273252,"version":"3.49.0"},"reference-count":51,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T00:00:00Z","timestamp":1610323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["20-14-00074"],"award-info":[{"award-number":["20-14-00074"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>In previous studies, we described a method for detecting and typing malignant tumors of the thyroid gland in fine-needle aspiration biopsy samples via analysis of a molecular marker panel (normalized HMGA2 mRNA level; normalized microRNA-146b, -221, and -375 levels; mitochondrial-to-nuclear DNA ratio; and BRAFV600E mutation) in cytological preparations by quantitative PCR. In the present study, we aimed to estimate the specificity of the typing of different thyroid tumors by the proposed method. Fine-needle aspiration cytological preparations from 278 patients were used. The histological diagnosis was known for each sample. The positive and negative predictive values of the method assessed in this study were, respectively, 100% and 98% for papillary thyroid carcinoma (n = 63), 100% and 100% for medullary thyroid carcinoma (n = 19), 43.5% and 98% for follicular carcinoma (n = 15), and 86% and 100% for H\u00fcrthle cell carcinoma (n = 6). Thus, we demonstrate that the diagnostic panel, including the analysis of microRNA expression, mRNA expression, the BRAFV600E mutation, and the mitochondrial-to-nuclear DNA ratio, allows the highly accurate identification of papillary thyroid carcinoma, medullary thyroid carcinoma, and H\u00fcrthle cell carcinoma but not malignant follicular tumors (positive predictive value was below 50%).<\/jats:p>","DOI":"10.3390\/cancers13020237","type":"journal-article","created":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T11:36:11Z","timestamp":1610364971000},"page":"237","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Preoperative Typing of Thyroid and Parathyroid Tumors with a Combined Molecular Classifier"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9401-5737","authenticated-orcid":false,"given":"Sergei E.","family":"Titov","sequence":"first","affiliation":[{"name":"Department of the Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia"},{"name":"AO Vector-Best, 630117 Novosibirsk, Russia"},{"name":"Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3659-7510","authenticated-orcid":false,"given":"Evgeniya S.","family":"Kozorezova","sequence":"additional","affiliation":[{"name":"National Center of Clinical Morphological Diagnostics, 192283 Saint Petersburg, Russia"},{"name":"Institute of Molecular Pathology and Pathomorphology, 630117 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9433-8341","authenticated-orcid":false,"given":"Pavel S.","family":"Demenkov","sequence":"additional","affiliation":[{"name":"Department of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia"},{"name":"Institute of Cytology and Genetics, SB RAS, 630090 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3799-9407","authenticated-orcid":false,"given":"Yulia A.","family":"Veryaskina","sequence":"additional","affiliation":[{"name":"Department of the Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia"},{"name":"Institute of Cytology and Genetics, SB RAS, 630090 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina V.","family":"Kuznetsova","sequence":"additional","affiliation":[{"name":"National Center of Clinical Morphological Diagnostics, 192283 Saint Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey L.","family":"Vorobyev","sequence":"additional","affiliation":[{"name":"National Center of Clinical Morphological Diagnostics, 192283 Saint Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman A.","family":"Chernikov","sequence":"additional","affiliation":[{"name":"National Center of Clinical Morphological Diagnostics, 192283 Saint Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilya V.","family":"Sleptsov","sequence":"additional","affiliation":[{"name":"Department of Endocrinology and Endocrine Surgery of Saint Petersburg State University N.I. Pirogov Clinic of High Medical Technologies, 190103 Saint Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nataliya I.","family":"Timofeeva","sequence":"additional","affiliation":[{"name":"National Center of Clinical Morphological Diagnostics, 192283 Saint Petersburg, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikhail K.","family":"Ivanov","sequence":"additional","affiliation":[{"name":"Department of the Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia"},{"name":"AO Vector-Best, 630117 Novosibirsk, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1634\/theoncologist.2007-0212","article-title":"Management of the solitary thyroid nodule","volume":"13","author":"Yeung","year":"2008","journal-title":"Oncologist"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1089\/thy.2009.0110","article-title":"Revised American Thyroid Association management guidelines for patients with thyroid nodules and differentiated thyroid cancer","volume":"11","author":"Cooper","year":"2009","journal-title":"Thyroid"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/thy.2015.0020","article-title":"2015 American Thyroid Association Management Guidelines for Adult Patients with Thyroid Nodules and Differentiated Thyroid Cancer: The American Thyroid Association Guidelines Task Force on Thyroid Nodules and Differentiated Thyroid Cancer","volume":"26","author":"Haugen","year":"2016","journal-title":"Thyroid"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.jacr.2017.01.046","article-title":"ACR Thyroid Imaging, Reporting and Data System (TI-RADS): White Paper of the ACR TI-RADS Committee","volume":"14","author":"Tessler","year":"2017","journal-title":"J. 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