{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T03:26:58Z","timestamp":1790047618700,"version":"4.0.1"},"reference-count":59,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T00:00:00Z","timestamp":1610496000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000325","name":"Wellbeing of Women","doi-asserted-by":"publisher","award":["RG1487 and RG2137"],"award-info":[{"award-number":["RG1487 and RG2137"]}],"id":[{"id":"10.13039\/501100000325","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009928","name":"Higher Committee for Education Development in Iraq","doi-asserted-by":"publisher","award":["RA and AK PhD studentships"],"award-info":[{"award-number":["RA and AK PhD studentships"]}],"id":[{"id":"10.13039\/501100009928","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Dyskerin is a core-component of the telomerase holo-enzyme, which elongates telomeres. Telomerase is involved in endometrial epithelial cell proliferation. Most endometrial cancers (ECs) have high telomerase activity; however, dyskerin expression in human healthy endometrium or in endometrial pathologies has not been investigated yet. We aimed to examine the expression, prognostic relevance, and functional role of dyskerin in human EC. Endometrial samples from a cohort of 175 women were examined with immunohistochemistry, immunoblotting, and qPCR. The EC cells were transfected with Myc-DDK-DKC1 plasmid and the effect of dyskerin overexpression on EC cell proliferation was assessed by flow cytometry. Human endometrium expresses dyskerin (DKC1) and dyskerin protein levels are significantly reduced in ECs when compared with healthy postmenopausal endometrium. Low dyskerin immunoscores were potentially associated with worse outcomes, suggesting a possible prognostic relevance. Cancer Genome Atlas (TCGA) ECs dataset (n = 589) was also interrogated. The TCGA dataset further confirmed changes in DKC1 expression in EC with prognostic significance. Transient dyskerin overexpression had a negative effect on EC cell proliferation. Our data demonstrates a role for dyskerin in normal endometrium for the first time and confirms aberrant expression with possible prognostic relevance in EC. Interventions aimed at modulating dyskerin levels may provide novel therapeutic options in EC.<\/jats:p>","DOI":"10.3390\/cancers13020273","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T11:52:32Z","timestamp":1610538752000},"page":"273","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Aberrant Dyskerin Expression Is Related to Proliferation and Poor Survival in Endometrial Cancer"],"prefix":"10.3390","volume":"13","author":[{"given":"Rafah","family":"Alnafakh","sequence":"first","affiliation":[{"name":"Liverpool Women\u2019s Hospital NHS Foundation Trust, Member of Liverpool Health Partners, Liverpool L8 7SS, UK"},{"name":"Department of Women\u2019s and Children\u2019s Health, Institute of Life Course and Medical Sciences, University of Liverpool, Member of Liverpool Health Partners, Liverpool L8 7SS, UK"},{"name":"Department of Pathology, Al-Hilla Teaching Hospital, Babil, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2100-8223","authenticated-orcid":false,"given":"Gabriele","family":"Saretzki","sequence":"additional","affiliation":[{"name":"Biosciences Institute, Campus for Ageing and Vitality, Newcastle University, Newcastle upon Tyne NE4 5PL, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Midgley","sequence":"additional","affiliation":[{"name":"Experimental Arthritis Treatment Centre for Children, Institute in the Park, Department of Women\u2019s and Children\u2019s Health, University of Liverpool, Liverpool L12 2AP, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Flynn","sequence":"additional","affiliation":[{"name":"Illumina Inc., San Diego, CA 92122, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Areege M.","family":"Kamal","sequence":"additional","affiliation":[{"name":"Department of Women\u2019s and Children\u2019s Health, Institute of Life Course and Medical Sciences, University of Liverpool, Member of Liverpool Health Partners, Liverpool L8 7SS, UK"},{"name":"Pathology Department, Oncology Teaching Hospital, Baghdad Medical City, Baghdad, Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Dobson","sequence":"additional","affiliation":[{"name":"Liverpool Women\u2019s Hospital NHS Foundation Trust, Member of Liverpool Health Partners, Liverpool L8 7SS, UK"},{"name":"Department of Women\u2019s and Children\u2019s Health, Institute of Life Course and Medical Sciences, University of Liverpool, Member of Liverpool Health Partners, Liverpool L8 7SS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Purushothaman","family":"Natarajan","sequence":"additional","affiliation":[{"name":"Liverpool Women\u2019s Hospital NHS Foundation Trust, Member of Liverpool Health Partners, Liverpool L8 7SS, UK"},{"name":"Department of Women\u2019s and Children\u2019s Health, Institute of Life Course and Medical Sciences, University of Liverpool, Member of Liverpool Health Partners, Liverpool L8 7SS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Stringfellow","sequence":"additional","affiliation":[{"name":"Lancashire Teaching Hospital NHS Trust, Preston PR2 9HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Martin-Hirsch","sequence":"additional","affiliation":[{"name":"Lancashire Teaching Hospital NHS Trust, Preston PR2 9HT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shandya B.","family":"DeCruze","sequence":"additional","affiliation":[{"name":"Liverpool Women\u2019s Hospital NHS Foundation Trust, Member of Liverpool Health Partners, Liverpool L8 7SS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1464-2069","authenticated-orcid":false,"given":"Sarah E.","family":"Coupland","sequence":"additional","affiliation":[{"name":"Molecular and Clinical Cancer Medicine, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L7 8TX, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0270-0150","authenticated-orcid":false,"given":"Dharani K.","family":"Hapangama","sequence":"additional","affiliation":[{"name":"Liverpool Women\u2019s Hospital NHS Foundation Trust, Member of Liverpool Health Partners, Liverpool L8 7SS, UK"},{"name":"Department of Women\u2019s and Children\u2019s Health, Institute of Life Course and Medical Sciences, University of Liverpool, Member of Liverpool Health Partners, Liverpool L8 7SS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2100","DOI":"10.1101\/gad.1346005","article-title":"Shelterin: The protein complex that shapes and safeguards human telomeres","volume":"19","year":"2005","journal-title":"Genes Dev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/S0092-8674(00)80760-6","article-title":"Mammalian Telomeres End in a Large Duplex Loop","volume":"97","author":"Griffith","year":"1999","journal-title":"Cell"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S0092-8674(00)80932-0","article-title":"TRF2 protects human telomeres from end-to-end fusions","volume":"92","author":"Smogorzewska","year":"1998","journal-title":"Cell"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/0022-2836(92)90096-3","article-title":"Telomere 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