{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:01:51Z","timestamp":1790744511350,"version":"4.1.0"},"reference-count":59,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T00:00:00Z","timestamp":1616544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Interreg POCTEFA program","award":["IMLINFO EFA281\/16"],"award-info":[{"award-number":["IMLINFO EFA281\/16"]}]},{"name":"R\u00e9gion Occitanie","award":["iMALC, n\u00b016003518"],"award-info":[{"award-number":["iMALC, n\u00b016003518"]}]},{"DOI":"10.13039\/501100002924","name":"FEDER","doi-asserted-by":"publisher","award":["iMALC, n\u00b016006947\/MP0008755"],"award-info":[{"award-number":["iMALC, n\u00b016006947\/MP0008755"]}],"id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Institut Claudius Regaud CLCC","award":["CIEL, R20027BB"],"award-info":[{"award-number":["CIEL, R20027BB"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Follicular lymphoma (FL) is an indolent B cell lymphoproliferative disorder of transformed follicular center B cells, which accounts for 20\u201330 percent of all non-Hodgkin lymphoma (NHL) cases. Great advances have been made to identify the most relevant targets for precision therapy. However, no relevant models for in vitro studies have been developed or characterized in depth. To this purpose, we generated a 3D cell model from t(14;18)-positive B-NHL cell lines cultured in ultra-low attachment 96-well plates. Morphological features and cell growth behavior were evaluated by classical microscopy (2D imaging) and response to treatment with different drugs was evaluated by a high-content analysis system to determine the robustness of the model. We show that the ultra-low attachment (ULA) method allows the development of regular, spherical and viable ULA-multicellular aggregates of lymphoma cells (MALC). However, discrepancies in the results obtained after 2D imaging analyses on drug-treated ULA-MALC prompted us to develop 3D imaging and specific analyses. We show by using light sheet microscopy and specifically developed 3D imaging algorithms that 3D imaging and dedicated analyses are necessary to characterize morphological properties of 3D models and drug effects. This study proposes a new method, but also imaging tools and informatic solutions, developed for FL necessary for future preclinical studies.<\/jats:p>","DOI":"10.3390\/cancers13071490","type":"journal-article","created":{"date-parts":[[2021,3,24]],"date-time":"2021-03-24T15:42:19Z","timestamp":1616600539000},"page":"1490","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["3D Model Characterization by 2D and 3D Imaging in t(14;18)-Positive B-NHL: Perspectives for In Vitro Drug Screens in Follicular Lymphoma"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1255-6121","authenticated-orcid":false,"given":"Fabien","family":"Gava","sequence":"first","affiliation":[{"name":"Centre de Recherches en Canc\u00e9rologie de Toulouse, INSERM UMR1037, CEDEX 1, 31037 Toulouse, France"},{"name":"Universit\u00e9 Toulouse III Paul-Sabatier, CEDEX 9, 31062 Toulouse, France"},{"name":"ERL 5294 CNRS, CEDEX 4, 31055 Toulouse, France"},{"name":"Institut Universitaire du Cancer-Oncopole de Toulouse, CEDEX 9, 31059 Toulouse, France"},{"name":"Laboratoire d\u2019Excellence \u2018TOUCAN-2\u2019, CEDEX 1, 31037 Toulouse, France"},{"name":"Institut Carnot Lymphome CALYM, 69495 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Faria","sequence":"additional","affiliation":[{"name":"Centre de Recherches en Canc\u00e9rologie de Toulouse, INSERM UMR1037, CEDEX 1, 31037 Toulouse, France"},{"name":"Universit\u00e9 Toulouse III Paul-Sabatier, CEDEX 9, 31062 Toulouse, France"},{"name":"ERL 5294 CNRS, CEDEX 4, 31055 Toulouse, France"},{"name":"Institut Universitaire du Cancer-Oncopole de Toulouse, CEDEX 9, 31059 Toulouse, France"},{"name":"Laboratoire d\u2019Excellence \u2018TOUCAN-2\u2019, CEDEX 1, 31037 Toulouse, France"},{"name":"Institut Carnot Lymphome CALYM, 69495 Pierre-B\u00e9nite, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0462-5652","authenticated-orcid":false,"given":"Pauline","family":"Gravelle","sequence":"additional","affiliation":[{"name":"Centre de Recherches en Canc\u00e9rologie de Toulouse, INSERM UMR1037, CEDEX 1, 31037 Toulouse, France"},{"name":"Universit\u00e9 Toulouse III Paul-Sabatier, CEDEX 9, 31062 Toulouse, France"},{"name":"ERL 5294 CNRS, CEDEX 4, 31055 Toulouse, France"},{"name":"Institut Universitaire du Cancer-Oncopole de Toulouse, CEDEX 9, 31059 Toulouse, France"},{"name":"Laboratoire d\u2019Excellence \u2018TOUCAN-2\u2019, CEDEX 1, 31037 Toulouse, France"},{"name":"Institut Carnot Lymphome CALYM, 69495 Pierre-B\u00e9nite, France"},{"name":"Department of Pathology, Institut Universitaire du Cancer de Toulouse, CEDEX 9, 31059 Toulouse, 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