{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T16:29:13Z","timestamp":1772468953757,"version":"3.50.1"},"reference-count":26,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T00:00:00Z","timestamp":1644019200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Ewing sarcoma is an aggressive and the second most common bone tumor in adolescent and young adult patients. The 5-year survival rate is 60\u201370% for localized disease but 30% for patients with metastases. Here, we aimed to identify a therapeutic target for Ewing sarcoma and evaluate antibody-based therapeutic agents using in vitro and in vivo models. We identified G protein-coupled receptor 64 (GPR64) as a therapeutic target for Ewing sarcoma via next-generation RNA-sequencing. GPR64v205 mRNA was expressed in HTB166, A673, MG63, 143B, HS-Sy II, and HT1080 cell lines as well as in Ewing sarcoma, undifferentiated pleomorphic sarcoma, leiomyosarcoma, dedifferentiated liposarcoma, and synovial sarcoma tissues. GPR64 expression was observed in 62.5% of sarcoma cases and was overexpressed in 33.9% cases. GPR64-specific monoclonal antibodies were tested as near-infrared probes for in vivo imaging using subcutaneous tumor mouse xenografts. Fluorescence intensity was stronger for the AF700-labeled anti-GPR64 antibody than that for the AF700-labeled isotype control antibody. GPR64 was detected in engrafted tumors of A673, 143B, HT1080, and the epididymis but not in other resected tissues. The anti-GPR64 antibody showed excellent binding to GPR64-positive tumors but not to healthy tissues. This antibody has potential for drug delivery in the antibody-based treatment of sarcomas.<\/jats:p>","DOI":"10.3390\/cancers14030814","type":"journal-article","created":{"date-parts":[[2022,2,6]],"date-time":"2022-02-06T20:36:47Z","timestamp":1644179807000},"page":"814","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["GPR64, Screened from Ewing Sarcoma Cells, Is a Potential Target for Antibody-Based Therapy for Various Sarcomas"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2330-7032","authenticated-orcid":false,"given":"Koichi","family":"Nakamura","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery, Graduate School of Medicine, Mie University, Tsu 514-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunihiro","family":"Asanuma","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Graduate School of Medicine, Mie University, Tsu 514-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3536-7008","authenticated-orcid":false,"given":"Takayuki","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keisuke","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Graduate School of Medicine, Mie University, Tsu 514-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yumi","family":"Matsuyama","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Graduate School of Medicine, Mie University, Tsu 514-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kouji","family":"Kita","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Graduate School of Medicine, Mie University, Tsu 514-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohito","family":"Hagi","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Graduate School of Medicine, Mie University, Tsu 514-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1099-7436","authenticated-orcid":false,"given":"Tomoki","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Graduate School of Medicine, Mie University, Tsu 514-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0474-4368","authenticated-orcid":false,"given":"Akihiro","family":"Sudo","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Graduate School of Medicine, Mie University, Tsu 514-8507, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.cancergen.2011.07.008","article-title":"Promiscuous partnerships in Ewing\u2019s sarcoma","volume":"204","author":"Sankar","year":"2011","journal-title":"Cancer Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1002\/pbc.24429","article-title":"Children\u2019s Oncology Group\u2019s 2013 blueprint for research: Bone tumors","volume":"60","author":"Gorlick","year":"2013","journal-title":"Pediatr. Blood Cancer"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1007\/s11864-009-0104-6","article-title":"Ewing\u2019s sarcoma: Standard and experimental treatment options","volume":"10","author":"Subbiah","year":"2009","journal-title":"Curr. Treat. Options Oncol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3603","DOI":"10.1073\/pnas.1521251113","article-title":"Exploring the surfaceome of Ewing sarcoma identifies a new and unique therapeutic target","volume":"113","author":"Town","year":"2016","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1038\/nri2744","article-title":"Monoclonal antibodies: Versatile platforms for cancer immunotherapy","volume":"10","author":"Weiner","year":"2010","journal-title":"Nat. Rev. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"453","DOI":"10.3389\/fonc.2018.00453","article-title":"CART immunotherapy: Development, success, and translation to malignant gliomas and other solid tumors","volume":"8","author":"Filley","year":"2018","journal-title":"Front. Oncol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1685","DOI":"10.1038\/nm.2554","article-title":"Cancer cell-selective in vivo near infrared photoimmunotherapy targeting specific membrane molecules","volume":"17","author":"Mitsunaga","year":"2011","journal-title":"Nat. Med."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kato, T., Wakiyama, H., Furusawa, A., Choyke, P.L., and Kobayashi, H. (2021). Near infrared photoimmunotherapy; a review of targets for cancer therapy. Cancers, 13.","DOI":"10.3390\/cancers13112535"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2500","DOI":"10.1056\/NEJMc1713444","article-title":"Tumor mutational burden and response rate to PD-1 inhibition","volume":"377","author":"Yarchoan","year":"2017","journal-title":"N. Engl. J. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1479-5876-11-6","article-title":"Chemokine CXCL14 is associated with prognosis in patients with colorectal carcinoma after curative resection","volume":"11","author":"Zeng","year":"2013","journal-title":"J. Transl. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.3748\/wjg.v23.i10.1780","article-title":"Clinicopathological significance of overexpression of interleukin-6 in colorectal cancer","volume":"23","author":"Zeng","year":"2017","journal-title":"World J. Gastroenterol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/bmt.2012.244","article-title":"Investigation of the freely available easy-to-use software \u2018EZR\u2019 for medical statistics","volume":"48","author":"Kanda","year":"2013","journal-title":"Bone Marrow Transplant."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1089\/dna.1997.16.379","article-title":"Cloning of a human epididymis-specific mRNA, HE6, encoding a novel member of the seven transmembrane-domain receptor superfamily","volume":"16","author":"Osterhoff","year":"1997","journal-title":"DNA Cell Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1038\/nrd.2017.178","article-title":"Trends in GPCR drug discovery: New agents, targets and indications","volume":"16","author":"Hauser","year":"2017","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1124\/pr.114.009647","article-title":"International Union of Basic and Clinical Pharmacology. XCIV. Adhesion G protein-coupled receptors","volume":"67","author":"Hamann","year":"2015","journal-title":"Pharmacol. Rev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1002\/path.4170","article-title":"G-protein coupled receptor 64 promotes invasiveness and metastasis in Ewing sarcomas through PGF and MMP1","volume":"230","author":"Richter","year":"2013","journal-title":"J. Pathol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/onc.2013.570","article-title":"Alternative splicing in cancer: Implications for biology and therapy","volume":"34","author":"Chen","year":"2015","journal-title":"Oncogene"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1715","DOI":"10.1101\/gad.1430906","article-title":"A Positive feedback loop couples ras activation and CD44 alternative splicing","volume":"20","author":"Cheng","year":"2006","journal-title":"Genes Dev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"629","DOI":"10.2217\/bmm-2017-0098","article-title":"Cancer-type OATP1B3 mRNA has the potential to become a detection and prognostic biomarker for human colorectal cancer","volume":"11","author":"Sun","year":"2017","journal-title":"Biomark. Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1002\/mrd.10220","article-title":"HE6, a two-subunit heptahelical receptor associated with apical membranes of efferent and epididymal duct epithelia","volume":"64","author":"Obermann","year":"2003","journal-title":"Mol. Reprod. Dev."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"9226","DOI":"10.1158\/0008-5472.CAN-05-1699","article-title":"Gene expression profiling of human sarcomas: Insights into sarcoma biology","volume":"65","author":"Baird","year":"2005","journal-title":"Cancer Res."},{"key":"ref_22","unstructured":"Monoclonal Antibodies (2012). Livertoxicol: Clinical and Research Information on Drug-Induced Liver Injury, National Institute of Diabetes and Digestive and Kidney Diseases."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8642","DOI":"10.1128\/MCB.24.19.8642-8648.2004","article-title":"Targeted deletion of the epididymal receptor HE6 results in fluid dysregulation and male infertility","volume":"24","author":"Davies","year":"2004","journal-title":"Mol. Cell. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e979619","DOI":"10.4161\/21565562.2014.979619","article-title":"The blood-epididymis barrier and inflammation","volume":"4","author":"Gregory","year":"2014","journal-title":"Spermatogenesis"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1530\/REP-08-0078","article-title":"HE6\/GPR64 Adhesion receptor co-localizes with apical and subapical f-actin scaffold in male excurrent duct epithelia","volume":"136","author":"Kirchhoff","year":"2008","journal-title":"Reproduction"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.mce.2005.12.023","article-title":"Role of Epididymal receptor he6 in the regulation of sperm microenvironment","volume":"250","author":"Kirchhoff","year":"2006","journal-title":"Mol. Cell. Endocrinol."}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/14\/3\/814\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:14:42Z","timestamp":1760134482000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/14\/3\/814"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,5]]},"references-count":26,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,2]]}},"alternative-id":["cancers14030814"],"URL":"https:\/\/doi.org\/10.3390\/cancers14030814","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,5]]}}}