{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:30:49Z","timestamp":1790699449695,"version":"4.1.0"},"reference-count":52,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T00:00:00Z","timestamp":1657670400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant","doi-asserted-by":"publisher","award":["06465-14"],"award-info":[{"award-number":["06465-14"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Low-magnitude (\u22641 g) high-frequency (\u226530 Hz) (LMHF) vibration has been shown to enhance bone mineral density. However, its regulation in breast cancer bone metastasis remains controversial for breast cancer patients and elder populations. Yoda1, an activator of the mechanosensitive Piezo1 channel, could potentially intensify the effect of LMHF vibration by enhancing the mechanoresponse of osteocytes, the major mechanosensory bone cells with high expression of Piezo1. In this study, we treated osteocytes with mono- (Yoda1 only or vibration only) or combined treatment (Yoda1 and LMHF vibration) and examined the further regulation of osteoclasts and breast cancer cells through the conditioned medium. Moreover, we studied the effects of combined treatment on breast cancer cells in regulation of osteocytes. Combined treatment on osteocytes showed beneficial effects, including increasing the nuclear translocation of Yes-associated protein (YAP) in osteocytes (488.0%, p &lt; 0.0001), suppressing osteoclastogenesis (34.3%, p = 0.004), and further reducing migration of MDA-MB-231 (15.1%, p = 0.02) but not Py8119 breast cancer cells (4.2%, p = 0.66). Finally, MDA-MB-231 breast cancer cells subjected to the combined treatment decreased the percentage of apoptotic osteocytes (34.5%, p = 0.04) but did not affect the intracellular calcium influx. This study showed the potential of stimulating Piezo1 in enhancing the mechanoresponse of osteocytes to LMHF vibration and further suppressing breast cancer migration via osteoclasts.<\/jats:p>","DOI":"10.3390\/cancers14143395","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T22:06:00Z","timestamp":1657749960000},"page":"3395","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Yoda1 Enhanced Low-Magnitude High-Frequency Vibration on Osteocytes in Regulation of MDA-MB-231 Breast Cancer Cell Migration"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4240-4148","authenticated-orcid":false,"given":"Chun-Yu","family":"Lin","sequence":"first","affiliation":[{"name":"Institute of Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0313-1631","authenticated-orcid":false,"given":"Xin","family":"Song","sequence":"additional","affiliation":[{"name":"Mechanical and Industrial Engineering, University of Toronto, Toronto, ON M5S 3G8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaji","family":"Ke","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjun","family":"Raha","sequence":"additional","affiliation":[{"name":"W Booth School of Engineering Practice and Technology, McMaster University, Hamilton, ON L8S 4L7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuning","family":"Wu","sequence":"additional","affiliation":[{"name":"W Booth School of Engineering Practice and Technology, McMaster University, Hamilton, ON L8S 4L7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murtaza","family":"Wasi","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, University of Delaware, Newark, DE 19716, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4332-262X","authenticated-orcid":false,"given":"Liyun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Mechanical Engineering, University of Delaware, Newark, DE 19716, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Geng","sequence":"additional","affiliation":[{"name":"W Booth School of Engineering Practice and Technology, McMaster University, Hamilton, ON L8S 4L7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidan","family":"You","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Engineering, University of Toronto, Toronto, ON M5S 3G9, Canada"},{"name":"Mechanical and Industrial Engineering, University of Toronto, Toronto, ON M5S 3G8, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1007\/s10555-014-9499-z","article-title":"Mimicking Breast Cancer-Induced Bone Metastasis in Vivo: Current Transplantation Models and Advanced Humanized Strategies","volume":"33","author":"Thibaudeau","year":"2014","journal-title":"Cancer Metastasis Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1038\/sj.cr.7290266","article-title":"Mechanisms of Cancer Metastasis to the Bone","volume":"15","author":"Yin","year":"2005","journal-title":"Cell Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1002\/jbmr.1966","article-title":"In Vivo Tibial Compression Decreases Osteolysis and Tumor Formation in a Human Metastatic Breast Cancer Model","volume":"28","author":"Lynch","year":"2013","journal-title":"J. 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