{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T12:22:35Z","timestamp":1768134155728,"version":"3.49.0"},"reference-count":53,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T00:00:00Z","timestamp":1663804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical Faculty of Kiel University","award":["AZ 70112935"],"award-info":[{"award-number":["AZ 70112935"]}]},{"name":"Medical Faculty of Kiel University","award":["01EY1103"],"award-info":[{"award-number":["01EY1103"]}]},{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"publisher","award":["AZ 70112935"],"award-info":[{"award-number":["AZ 70112935"]}],"id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"publisher","award":["01EY1103"],"award-info":[{"award-number":["01EY1103"]}],"id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"German Federal Ministry of Education and Research","doi-asserted-by":"publisher","award":["AZ 70112935"],"award-info":[{"award-number":["AZ 70112935"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"German Federal Ministry of Education and Research","doi-asserted-by":"publisher","award":["01EY1103"],"award-info":[{"award-number":["01EY1103"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive solid malignancies with poor survival rates. Only 20% of the patients are eligible for R0-surgical resection, presenting with early relapses, mainly in the liver. PDAC patients with hepatic metastases have a worse outcome compared to patients with metastases at other sites. Early detection of hepatic spread bears the potential to improve patient outcomes. Thus, this study sought for serum-based perioperative biomarkers allowing discrimination of early (EHMS \u2264 12 months) and late hepatic metastatic spread (LHMS &gt; 12 months). Serum samples from 83 resectable PDAC patients were divided into EHMS and LHMS and analyzed for levels of inflammatory mediators by LEGENDplexTM, which was validated and extended by Olink\u00ae analysis. CA19-9 serum levels served as control. Results were correlated with clinicopathological data. While serum CA19-9 levels were comparable, Olink\u00ae analysis confirmed distinct differences between both groups. It revealed significantly elevated levels of factors involved in chemotaxis and migration of immune cells, immune activity, and cell growth in serum of LHMS-patients. Overall, Olink\u00ae analysis identified a comprehensive biomarker panel in serum of PDAC patients that could provide the basis for predicting LHMS. However, further studies with larger cohorts are required for its clinical translation.<\/jats:p>","DOI":"10.3390\/cancers14194605","type":"journal-article","created":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T21:10:05Z","timestamp":1663881005000},"page":"4605","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Biomarkers in Liquid Biopsies for Prediction of Early Liver Metastases in Pancreatic Cancer"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4415-8371","authenticated-orcid":false,"given":"Anne-Sophie","family":"Mehdorn","sequence":"first","affiliation":[{"name":"Department of General, Visceral, Thoracic, Transplantation and Pediatric Surgery, University Hospital Schleswig-Holstein Campus Kiel, Arnold-Heller-Str. 3, Building C, 24105 Kiel, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7400-5240","authenticated-orcid":false,"given":"Timo","family":"Gemoll","sequence":"additional","affiliation":[{"name":"Section for Translational Surgical Oncology and Biobanking, Department of Surgery, University Medical Center Schleswig-Holstein, University of L\u00fcbeck, Campus L\u00fcbeck, Ratzeburger Allee 160, 23562 L\u00fcbeck, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4763-4521","authenticated-orcid":false,"given":"Hauke","family":"Busch","sequence":"additional","affiliation":[{"name":"Group Medical Systems Biology, University of L\u00fcbeck, Ratzeburger Allee 160, 23538 L\u00fcbeck, Germany"},{"name":"Institute for Experimental Dermatology, University of L\u00fcbeck, Ratzeburger Allee 160, 23562 L\u00fcbeck, Germany"},{"name":"Institute for Cardiogenetics, University of L\u00fcbeck, Building 67, Ratzeburger Allee 160, 23562 L\u00fcbeck, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0560-9301","authenticated-orcid":false,"given":"Katharina","family":"Kern","sequence":"additional","affiliation":[{"name":"Section for Translational Surgical Oncology and Biobanking, Department of Surgery, University Medical Center Schleswig-Holstein, University of L\u00fcbeck, Campus L\u00fcbeck, Ratzeburger Allee 160, 23562 L\u00fcbeck, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silje","family":"Beckinger","sequence":"additional","affiliation":[{"name":"Institute for Experimental Cancer Research, University Hospital Schleswig-Holstein Campus Kiel, Kiel University, Arnold-Heller-Str. 3, Building U30 Entrance 1, 24105 Kiel, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1062-1076","authenticated-orcid":false,"given":"Tina","family":"Daunke","sequence":"additional","affiliation":[{"name":"Institute for Experimental Cancer Research, University Hospital Schleswig-Holstein Campus Kiel, Kiel University, Arnold-Heller-Str. 3, Building U30 Entrance 1, 24105 Kiel, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4124-7918","authenticated-orcid":false,"given":"Christoph","family":"Kahlert","sequence":"additional","affiliation":[{"name":"Department for Visceral, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technical University Dresden, Building 59, Fetscherstra\u00dfe 74, 01307 Dresden, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6890-4829","authenticated-orcid":false,"given":"Faik G.","family":"Uzunoglu","sequence":"additional","affiliation":[{"name":"General, Visceral and Thoracic Surgery Department and Clinic, University Medical Center, Hamburg-Eppendorf, Martinistra\u00dfe 52, 20246 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Hendricks","sequence":"additional","affiliation":[{"name":"Department of General, Thorax, Vascular and Transplant Surgery, University Hospital Rostock, Schillingallee 35, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9927-1852","authenticated-orcid":false,"given":"Florian","family":"Buertin","sequence":"additional","affiliation":[{"name":"Department of General, Thorax, Vascular and Transplant Surgery, University Hospital Rostock, Schillingallee 35, 18057 Rostock, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6194-2374","authenticated-orcid":false,"given":"Uwe A.","family":"Wittel","sequence":"additional","affiliation":[{"name":"Department of General and Visceral Surgery, Centre of Surgery, Medical Centre, Faculty of Medicine, University of Freiburg, Breisacher Str. 86, 79110 Freiburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1235-7512","authenticated-orcid":false,"given":"Yoshiaki","family":"Sunami","sequence":"additional","affiliation":[{"name":"Department of Visceral, Vascular and Endocrine Surgery, Martin-Luther-University Halle-Wittenberg University Medical Center Halle, Ernst-Grube-Stra\u00dfe 40, 06120 Halle, 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