{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T01:49:35Z","timestamp":1783648175796,"version":"3.55.0"},"reference-count":67,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T00:00:00Z","timestamp":1664150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondation Roi Baudouin (Fund Yvonne Smits)","award":["ARC 15\/20-065"],"award-info":[{"award-number":["ARC 15\/20-065"]}]},{"name":"Universit\u00e9 Catholique de Louvain","award":["ARC 15\/20-065"],"award-info":[{"award-number":["ARC 15\/20-065"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Papillary thyroid carcinoma (PTC) is the most frequent histological subtype of thyroid cancers (TC), and BRAFV600E genetic alteration is found in 60% of this endocrine cancer. This oncogene is associated with poor prognosis, resistance to radioiodine therapy, and tumor progression. Histological follow-up by anatomo-pathologists revealed that two-thirds of surgically-removed thyroids do not present malignant lesions. Thus, continued fundamental research into the molecular mechanisms of TC downstream of BRAFV600E remains central to better understanding the clinical behavior of these tumors. To study PTC, we used a mouse model in which expression of BRAFV600E was specifically switched on in thyrocytes by doxycycline administration. Upon daily intraperitoneal doxycycline injection, thyroid tissue rapidly acquired histological features mimicking human PTC. Transcriptomic analysis revealed major changes in immune signaling pathways upon BRAFV600E induction. Multiplex immunofluorescence confirmed the abundant recruitment of macrophages, among which a population of LYVE-1+\/CD206+\/STABILIN-1+ was dramatically increased. By genetically inactivating the gene coding for the scavenger receptor STABILIN-1, we showed an increase of CD8+ T cells in this in situ BRAFV600E-dependent TC. Lastly, we demonstrated the presence of CD206+\/STABILIN-1+ macrophages in human thyroid pathologies. Altogether, we revealed the recruitment of immunosuppressive STABILIN-1 macrophages in a PTC mouse model and the interest to further study this macrophage subpopulation in human thyroid tissues.<\/jats:p>","DOI":"10.3390\/cancers14194687","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T21:23:27Z","timestamp":1664313807000},"page":"4687","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["BRAFV600E Expression in Thyrocytes Causes Recruitment of Immunosuppressive STABILIN-1 Macrophages"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6799-1879","authenticated-orcid":false,"given":"Catherine","family":"Spourquet","sequence":"first","affiliation":[{"name":"CELL Unit, de Duve Institute, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oph\u00e9lie","family":"Delcorte","sequence":"additional","affiliation":[{"name":"CELL Unit, de Duve Institute, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Lemoine","sequence":"additional","affiliation":[{"name":"CELL Unit, de Duve Institute, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4416-9903","authenticated-orcid":false,"given":"Nicolas","family":"Dauguet","sequence":"additional","affiliation":[{"name":"CYTF Platform, de Duve Institute, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axelle","family":"Loriot","sequence":"additional","affiliation":[{"name":"CBIO Unit, de Duve Institute, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7409-1441","authenticated-orcid":false,"given":"Younes","family":"Achouri","sequence":"additional","affiliation":[{"name":"Transgenesis Platform, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3250-7653","authenticated-orcid":false,"given":"Maija","family":"Hollm\u00e9n","sequence":"additional","affiliation":[{"name":"MediCity Research Laboratory and InFLAMES Flagship, University of Turku, 20500 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirpa","family":"Jalkanen","sequence":"additional","affiliation":[{"name":"MediCity Research Laboratory and InFLAMES Flagship, University of Turku, 20500 Turku, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Huaux","sequence":"additional","affiliation":[{"name":"LTAP Unit, IREC, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Lucas","sequence":"additional","affiliation":[{"name":"GECE Unit, de Duve Institute, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"},{"name":"Walloon Excellence in Life Sciences and Biotechnology (WELBIO), 1300 Wavre, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre Van","family":"Meerkeeck","sequence":"additional","affiliation":[{"name":"GECE Unit, de Duve Institute, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey A.","family":"Knauf","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head & Neck Surgery in the Cleveland Clinic Lerner, College of Medicine of Case Western Reserve University, Cleveland, OH 44106, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Fagin","sequence":"additional","affiliation":[{"name":"Department of Medicine and Human Oncology & Pathogenesis Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9386-0442","authenticated-orcid":false,"given":"Chantal","family":"Dessy","sequence":"additional","affiliation":[{"name":"FATH & MORF Unit, IREC, Universit\u00e9 Catholique de Louvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Mourad","sequence":"additional","affiliation":[{"name":"Surgery and Abdominal Transplantation Division, Cliniques Universitaires Saint-Luc, Universit\u00e9 Catholique de Louvain, 1200 Brussels, 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