{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:21:37Z","timestamp":1790846497750,"version":"4.1.0"},"reference-count":28,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T00:00:00Z","timestamp":1672012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Higher Education of the Russian Federation","award":["0714-2020-0006"],"award-info":[{"award-number":["0714-2020-0006"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Diffuse gliomas continue to be an important problem in neuro-oncology. To solve it, studies have considered the issues of molecular pathogenesis from the intratumoral heterogeneity point. Here, we carried out a comparative dynamic analysis of the different cell populations\u2019 content in diffuse gliomas of different molecular profiles and grades, considering the cell populations\u2019 functional properties and the relationship with patient survival, using flow cytometry, immunofluorescence, multiparametric fluorescent in situ hybridization, polymerase chain reaction, and cultural methods. It was shown that an increase in the IDH-mutant astrocytomas and oligodendrogliomas malignancy is accompanied by an increase in stem cells\u2019 proportion and mesenchymal cell populations\u2019 appearance arising from oligodendrocyte-progenitor-like cells with cell plasticity and cells\u2019 hypoxia response programs\u2019 activation. In glioblastomas, malignancy increase is accompanied by an increase in both stem and definitive cells with mesenchymal differentiation, while proneuronal glioma stem cells are the most likely the source of mesenchymal glioma stem cells, which, in hypoxic conditions, further give rise to mesenchymal-like cells. Clinical confirmation was a mesenchymal-like cell and mesenchymal glioma stem cell number, and the hypoxic and plastic molecular programs\u2019 activation degree had a significant effect on relapse-free and overall survival. In general, we built a multi-vector model of diffuse gliomas\u2019 pathogenetic tracing up to the practical plane.<\/jats:p>","DOI":"10.3390\/cancers15010145","type":"journal-article","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T02:53:11Z","timestamp":1672109591000},"page":"145","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cell-Population Dynamics in Diffuse Gliomas during Gliomagenesis and Its Impact on Patient Survival"],"prefix":"10.3390","volume":"15","author":[{"given":"Pavel V.","family":"Nikitin","sequence":"first","affiliation":[{"name":"University of Oslo, 0315 Oslo, Norway"},{"name":"Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, 119991 Moscow, Russia"},{"name":"Center for Brain and Neurotechnology, 117513 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guzel R.","family":"Musina","sequence":"additional","affiliation":[{"name":"University of Oslo, 0315 Oslo, Norway"},{"name":"Center for Brain and Neurotechnology, 117513 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9622-3457","authenticated-orcid":false,"given":"Stanislav I.","family":"Pekov","sequence":"additional","affiliation":[{"name":"Skolkovo Institute of Science and Technology, 121205 Moscow, Russia"},{"name":"Siberian State Medical University, 634050 Tomsk, Russia"},{"name":"Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8462-9302","authenticated-orcid":false,"given":"Andrey A.","family":"Kuzin","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor A.","family":"Popov","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artem Y.","family":"Belyaev","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory L.","family":"Kobyakov","sequence":"additional","affiliation":[{"name":"Burdenko Neurosurgical Center, 125047 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry Y.","family":"Usachev","sequence":"additional","affiliation":[{"name":"Burdenko Neurosurgical Center, 125047 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viktor N.","family":"Nikolaev","sequence":"additional","affiliation":[{"name":"N.N. Blokhin Cancer Research Center (RCRC), 115522 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin P.","family":"Mikhailov","sequence":"additional","affiliation":[{"name":"N.N. Blokhin Cancer Research Center (RCRC), 115522 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1200\/JCO.21.02036","article-title":"Therapy for Diffuse Astrocytic and Oligodendroglial Tumors in Adults: ASCO-SNO Guideline","volume":"40","author":"Mohile","year":"2022","journal-title":"J. Clin. 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