{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T01:43:28Z","timestamp":1779155008977,"version":"3.51.4"},"reference-count":43,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T00:00:00Z","timestamp":1677715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Cancer Research UK","award":["C197\/A29580"],"award-info":[{"award-number":["C197\/A29580"]}]},{"name":"Cancer Research UK","award":["C197\/A17242"],"award-info":[{"award-number":["C197\/A17242"]}]},{"name":"Cancer Research UK","award":["C197\/A28289"],"award-info":[{"award-number":["C197\/A28289"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>There is an unmet clinical need for imaging agents capable of detecting early evidence of tumor cell death, since the timing, extent, and distribution of cell death in tumors following treatment can give an indication of treatment outcome. We describe here 68Ga-labeled C2Am, which is a phosphatidylserine-binding protein, for imaging tumor cell death in vivo using positron emission tomography (PET). A one-pot synthesis of 68Ga-C2Am (20 min, 25 \u00b0C, &gt;95% radiochemical purity) has been developed, using a NODAGA-maleimide chelator. The binding of 68Ga-C2Am to apoptotic and necrotic tumor cells was assessed in vitro using human breast and colorectal cancer cell lines, and in vivo, using dynamic PET measurements in mice implanted subcutaneously with the colorectal tumor cells and treated with a TRAIL-R2 agonist. 68Ga-C2Am showed predominantly renal clearance and low retention in the liver, spleen, small intestine, and bone and generated a tumor-to-muscle (T\/m) ratio of 2.3 \u00b1 0.4, at 2 h post probe administration and at 24 h following treatment. 68Ga-C2Am has the potential to be used in the clinic as a PET tracer for assessing early treatment response in tumors.<\/jats:p>","DOI":"10.3390\/cancers15051564","type":"journal-article","created":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T01:43:00Z","timestamp":1677807780000},"page":"1564","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Preclinical PET Imaging of Tumor Cell Death following Therapy Using Gallium-68-Labeled C2Am"],"prefix":"10.3390","volume":"15","author":[{"given":"Flaviu","family":"Bulat","sequence":"first","affiliation":[{"name":"Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge CB2 1TN, UK"},{"name":"Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5427-7564","authenticated-orcid":false,"given":"Friederike","family":"Hesse","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge CB2 1TN, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bala","family":"Attili","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge CB2 1TN, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chandra","family":"Solanki","sequence":"additional","affiliation":[{"name":"Addenbrooke\u2019s Hospital Radiopharmacy, Cambridge University Hospitals NHS Foundation Trust, Cambridge CB2 0QQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3777-2827","authenticated-orcid":false,"given":"Iosif A.","family":"Mendichovszky","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, Cambridge University Hospitals NHS Foundation Trust, Cambridge CB2 0QQ, UK"},{"name":"Department of Radiology, University of Cambridge, Cambridge CB2 1EW, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franklin","family":"Aigbirhio","sequence":"additional","affiliation":[{"name":"Wolfson Brain Imaging Centre, University of Cambridge, Cambridge CB2 0QQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3408-5199","authenticated-orcid":false,"given":"Finian J.","family":"Leeper","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3883-6287","authenticated-orcid":false,"given":"Kevin M.","family":"Brindle","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge CB2 1TN, UK"},{"name":"Department of Biochemistry, University of Cambridge, Cambridge CB2 1GA, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2740-5166","authenticated-orcid":false,"given":"Andr\u00e9 A.","family":"Neves","sequence":"additional","affiliation":[{"name":"Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge CB2 1TN, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/nrc2289","article-title":"New Approaches for Imaging Tumour Responses to Treatment","volume":"8","author":"Brindle","year":"2008","journal-title":"Nat. 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