{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:29:06Z","timestamp":1792985346903,"version":"4.5.0"},"reference-count":90,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T00:00:00Z","timestamp":1681776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002794","name":"Swedish Cancer Foundation","doi-asserted-by":"publisher","award":["S19 0238 Pj"],"award-info":[{"award-number":["S19 0238 Pj"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Swedish Cancer Foundation","doi-asserted-by":"publisher","award":["FBKS-2019-14-(210)"],"award-info":[{"award-number":["FBKS-2019-14-(210)"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Swedish Cancer Foundation","doi-asserted-by":"publisher","award":["847583"],"award-info":[{"award-number":["847583"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Berta Kamprad Foundation","award":["S19 0238 Pj"],"award-info":[{"award-number":["S19 0238 Pj"]}]},{"name":"Berta Kamprad Foundation","award":["FBKS-2019-14-(210)"],"award-info":[{"award-number":["FBKS-2019-14-(210)"]}]},{"name":"Berta Kamprad Foundation","award":["847583"],"award-info":[{"award-number":["847583"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program","award":["S19 0238 Pj"],"award-info":[{"award-number":["S19 0238 Pj"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program","award":["FBKS-2019-14-(210)"],"award-info":[{"award-number":["FBKS-2019-14-(210)"]}]},{"name":"European Union\u2019s Horizon 2020 Research and Innovation Program","award":["847583"],"award-info":[{"award-number":["847583"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Mycosis fungoides (MF) and S\u00e9zary syndrome (SS) are two closely related clinical variants of cutaneous T-cell lymphomas (CTCL). Previously demonstrated large patient-to-patient and intra-patient disease heterogeneity underpins the importance of personalized medicine in CTCL. Advanced stages of CTCL are characterized by dismal prognosis, and the early identification of patients who will progress remains a clinical unmet need. While the exact molecular events underlying disease progression are poorly resolved, the tumor microenvironment (TME) has emerged as an important driver. In particular, the Th1-to-Th2 shift in the immune response is now commonly identified across advanced-stage CTCL patients. Herein, we summarize the role of the TME in CTCL evolution and the latest studies in deciphering inter- and intra-patient heterogeneity. We introduce spatially resolved omics as a promising technology to advance immune-oncology efforts in CTCL. We propose the combined implementation of spatially guided and single-cell omics technologies in paired skin and blood samples. Such an approach will mediate in-depth profiling of phenotypic and molecular changes in reactive immune subpopulations and malignant T cells preceding the Th1-to-Th2 shift and reveal mechanisms underlying disease progression from skin-limited to systemic disease that collectively will lead to the discovery of novel biomarkers to improve patient prognostication and the design of personalized treatment strategies.<\/jats:p>","DOI":"10.3390\/cancers15082362","type":"journal-article","created":{"date-parts":[[2023,4,19]],"date-time":"2023-04-19T01:09:22Z","timestamp":1681866562000},"page":"2362","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Spatially Guided and Single Cell Tools to Map the Microenvironment in Cutaneous T-Cell Lymphoma"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8772-0729","authenticated-orcid":false,"given":"Eirini","family":"Kalliara","sequence":"first","affiliation":[{"name":"Department of Immunotechnology, Faculty of Engineering (LTH), University of Lund, 223 63 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1717-0842","authenticated-orcid":false,"given":"Emma","family":"Belfrage","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Skane University Hospital (SUS), 205 02 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urban","family":"Gullberg","sequence":"additional","affiliation":[{"name":"Department of Hematology and Transfusion Medicine, Skane University Hospital (SUS), 205 02 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Drott","sequence":"additional","affiliation":[{"name":"Department of Hematology and Transfusion Medicine, Skane University Hospital (SUS), 205 02 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1388-4912","authenticated-orcid":false,"given":"Sara","family":"Ek","sequence":"additional","affiliation":[{"name":"Department of Immunotechnology, Faculty of Engineering (LTH), University of Lund, 223 63 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,18]]},"reference":[{"key":"ref_1","first-page":"1112","article-title":"The 2018 update of the WHO-EORTC classification for primary cutaneous lymphomas","volume":"134","author":"Willemze","year":"2019","journal-title":"Blood"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1002\/hon.2584","article-title":"Cutaneous lymphomas\u2014An update 2019","volume":"37","author":"Kempf","year":"2019","journal-title":"Hematol. 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