{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:03:03Z","timestamp":1792803783951,"version":"4.5.0"},"reference-count":39,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:00:00Z","timestamp":1689724800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Danish Cancer Society","award":["2021 R322-A17350"],"award-info":[{"award-number":["2021 R322-A17350"]}],"id":[{"id":"https:\/\/ror.org\/03ytt7k16","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Danish Cancer Society","award":["101079210"],"award-info":[{"award-number":["101079210"]}],"id":[{"id":"https:\/\/ror.org\/03ytt7k16","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Research Executive Agency","award":["2021 R322-A17350"],"award-info":[{"award-number":["2021 R322-A17350"]}],"id":[{"id":"https:\/\/ror.org\/05mkt9r11","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Research Executive Agency","award":["101079210"],"award-info":[{"award-number":["101079210"]}],"id":[{"id":"https:\/\/ror.org\/05mkt9r11","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Arvid Nilssons Fond","award":["2021 R322-A17350"],"award-info":[{"award-number":["2021 R322-A17350"]}],"id":[{"id":"https:\/\/ror.org\/00a59ax11","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Arvid Nilssons Fond","award":["101079210"],"award-info":[{"award-number":["101079210"]}],"id":[{"id":"https:\/\/ror.org\/00a59ax11","id-type":"ROR","asserted-by":"crossref"}]},{"name":"T\u00f8mrermester J\u00f8rgen Holm og hustru, Elisa f. Hansens Mindelegat, Dagmar Marshalls Fond","award":["2021 R322-A17350"],"award-info":[{"award-number":["2021 R322-A17350"]}]},{"name":"T\u00f8mrermester J\u00f8rgen Holm og hustru, Elisa f. Hansens Mindelegat, Dagmar Marshalls Fond","award":["101079210"],"award-info":[{"award-number":["101079210"]}]},{"name":"L\u00e6geforeningens Forskningsfond","award":["2021 R322-A17350"],"award-info":[{"award-number":["2021 R322-A17350"]}]},{"name":"L\u00e6geforeningens Forskningsfond","award":["101079210"],"award-info":[{"award-number":["101079210"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Cancer-associated fibroblasts (CAFs) have been shown to impact the chemosensitivity of patient-derived tumor organoids (PDTOs). However, the published literature comparing PDTO response to clinical outcome does not include CAFs in the models. Here, a co-culture model was created using PDTOs and CAFs derived from endoscopic ultrasound-guided fine-needle biopsies (EUS-FNBs) for potential use in drug screening applications. Co-cultures were established, and growth was compared to monocultures using image metrics and a commercially available assay. We were able to establish and expand validated malignant PDTOs from 19.2% of adenocarcinomas from EUS-FNBs. CAFs could be established from 25% of the samples. The viability of PDTOs in the mixed cell co-culture could be isolated using image metrics. The addition of CAFs promoted PDTO growth in half of the established co-cultures. These results show that co-cultures can be established from tiny amounts of tissue provided by EUS-FNB. An increased growth of PDTOs was shown in co-cultures, suggesting that the present setup successfully models CAF\u2013PDTO interaction. Furthermore, we demonstrated that standard validation techniques may be insufficient to detect contamination with normal cells in PDTO cultures established from primary tumor core biopsies.<\/jats:p>","DOI":"10.3390\/cancers15143677","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T21:21:46Z","timestamp":1689801706000},"page":"3677","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Validation of a Novel EUS-FNB-Derived Organoid Co-Culture System for Drug Screening in Patients with Pancreatic Cancer"],"prefix":"10.3390","volume":"15","author":[{"given":"Simon Ezban","family":"Gr\u00fctzmeier","sequence":"first","affiliation":[{"name":"Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, 2730 Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3941-0544","authenticated-orcid":false,"given":"Bojan","family":"Kovacevic","sequence":"additional","affiliation":[{"name":"Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, 2730 Herlev, Denmark"},{"name":"Department of Surgery and Transplantation, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Vilmann","sequence":"additional","affiliation":[{"name":"Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, 2730 Herlev, Denmark"},{"name":"Department of Clinical Medicine, University of Copenhagen, 2200 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte Vestrup","family":"Rift","sequence":"additional","affiliation":[{"name":"Department of Pathology, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linea Cecilie","family":"Melchior","sequence":"additional","affiliation":[{"name":"Department of Pathology, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten Orebo","family":"Holmstr\u00f6m","sequence":"additional","affiliation":[{"name":"National Center for Cancer Immune Therapy, Department of Oncology, Copenhagen University Hospital Herlev and Gentofte, 2730 Herlev, Denmark"},{"name":"Department of Immunology and Microbiology, University of Copenhagen, 2200 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lene","family":"Brink","sequence":"additional","affiliation":[{"name":"Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, 2730 Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hazem","family":"Hassan","sequence":"additional","affiliation":[{"name":"Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, 2730 Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John G\u00e1sdal","family":"Karstensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, University of Copenhagen, 2200 Copenhagen, Denmark"},{"name":"Pancreatitis Centre East, Gastroenterology Unit, Copenhagen University Hospital\u2014Amager and Hvidovre, 2650 Hvidovre, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne","family":"Grossjohann","sequence":"additional","affiliation":[{"name":"Department of Surgery and Transplantation, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepthi","family":"Chiranth","sequence":"additional","affiliation":[{"name":"Department of Pathology, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Toxv\u00e6rd","sequence":"additional","affiliation":[{"name":"Department of Pathology, Copenhagen University Hospital Herlev and Gentofte, 2730 Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9256-2240","authenticated-orcid":false,"given":"Carsten Paln\u00e6s","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Surgery and Transplantation, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4689-5658","authenticated-orcid":false,"given":"Estrid","family":"H\u00f8gdall","sequence":"additional","affiliation":[{"name":"Department of Pathology, Copenhagen University Hospital Herlev and Gentofte, 2730 Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane Preuss","family":"Hasselby","sequence":"additional","affiliation":[{"name":"Department of Pathology, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"},{"name":"Department of Immunology and Microbiology, University of Copenhagen, 2200 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pia","family":"Klausen","sequence":"additional","affiliation":[{"name":"Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, 2730 Herlev, Denmark"},{"name":"Department of Pathology, Copenhagen University Hospital Rigshospitalet, 2100 Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3322\/caac.21763","article-title":"Cancer statistics, 2023","volume":"73","author":"Siegel","year":"2023","journal-title":"CA Cancer J. 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