{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T03:23:03Z","timestamp":1790133783712,"version":"4.0.1"},"reference-count":35,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T00:00:00Z","timestamp":1691452800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Purpose: To assess the potential value of LiqBio as a complementary tool for diagnosis and surveillance of BCL. Methods: This prospective multi-center study included 78 patients (25 follicular lymphomas (FL) and 53 large B-cell lymphomas (LBCL)). We performed next-generation sequencing (NGS) of cfDNA LiqBio and paired gDNA tissue biopsies at diagnosis and compared the mutational statuses. Also, through NGS of LiqBio, we identified MRD biomarkers and compared this novel LiqBio\u2013MRD method with PET\/CT in detecting MRD at follow-up. Results: We identified mutations in 71% of LiqBio and 95% of tissue biopsies, and found a correlation between variant allele frequency of somatic mutations. Additionally, we identified mutations in 73% of LiqBio from patients with no available tissue samples or no mutations in them. Regarding the utility of LiqBio\u2013MRD as a dynamic monitoring tool, when compared with the PET\/CT method, a lower sensitivity was observed for LiqBio\u2013MRD at 92.3% (vs. 100% for PET\/CT), but a higher specificity of 91.3% (vs. 86.9% for PET\/CT). Conclusion: Genetic profiling of tumor cfDNA in plasma LiqBio is a complementary tool for BCL diagnosis and MRD surveillance.<\/jats:p>","DOI":"10.3390\/cancers15164022","type":"journal-article","created":{"date-parts":[[2023,8,8]],"date-time":"2023-08-08T12:38:59Z","timestamp":1691498339000},"page":"4022","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genetic Profiling of Cell-Free DNA in Liquid Biopsies: A Complementary Tool for the Diagnosis of B-Cell Lymphomas and the Surveillance of Measurable Residual Disease"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4031-9601","authenticated-orcid":false,"given":"Gloria","family":"Figaredo","sequence":"first","affiliation":[{"name":"Department of Haematology, Hospital Universitario de Toledo, Av. del R\u00edo Guadiana, 45007 Toledo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5180-5760","authenticated-orcid":false,"given":"Alejandro","family":"Mart\u00edn-Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Altum Sequencing SL, Av. Gregorio Peces Barba, 1, 28919 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago","family":"Barrio","sequence":"additional","affiliation":[{"name":"Altum Sequencing SL, Av. Gregorio Peces Barba, 1, 28919 Madrid, Spain"},{"name":"Computational Science Department, Carlos III University, Ronda de Toledo, 1, 28005 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4740-8057","authenticated-orcid":false,"given":"Laura","family":"Parrilla","sequence":"additional","affiliation":[{"name":"Department of Haematology, Hospital Universitario de Toledo, Av. del R\u00edo Guadiana, 45007 Toledo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"Campos-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Biobank Department, Hospital Universitario de Toledo, Av. del R\u00edo Guadiana, 45007 Toledo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Poza","sequence":"additional","affiliation":[{"name":"Haematology Department, Hospital Universitario 12 de Octubre, Avda. de C\u00f3rdoba, s\/n, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Rufi\u00e1n","sequence":"additional","affiliation":[{"name":"Altum Sequencing SL, Av. Gregorio Peces Barba, 1, 28919 Madrid, Spain"},{"name":"Haematology Department, Hospital Universitario 12 de Octubre, Avda. de C\u00f3rdoba, s\/n, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrocinio","family":"Algara","sequence":"additional","affiliation":[{"name":"Genetics Department, Hospital Universitario de Toledo, Av. del R\u00edo Guadiana, 45007 Toledo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"De La Torre","sequence":"additional","affiliation":[{"name":"Department of Haematology, Hospital Universitario de Toledo, Av. del R\u00edo Guadiana, 45007 Toledo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Jim\u00e9nez Ubieto","sequence":"additional","affiliation":[{"name":"Haematology Department, Hospital Universitario 12 de Octubre, Avda. de C\u00f3rdoba, s\/n, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7908-0063","authenticated-orcid":false,"given":"Joaqu\u00edn","family":"Mart\u00ednez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Haematology Department, Hospital Universitario 12 de Octubre, Avda. de C\u00f3rdoba, s\/n, 28041 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis-Felipe","family":"Casado","sequence":"additional","affiliation":[{"name":"Department of Haematology, Hospital Universitario de Toledo, Av. del R\u00edo Guadiana, 45007 Toledo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela","family":"Mollejo","sequence":"additional","affiliation":[{"name":"Anatomopathology Department, Hospital Universitario de Toledo, Av. del R\u00edo Guadiana, 45007 Toledo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10238-021-00718-8","article-title":"Prognostic value of circulating tumor DNA in lymphoma: A meta-analysis","volume":"22","author":"Yao","year":"2021","journal-title":"Clin. 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