{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T23:53:24Z","timestamp":1785887604119,"version":"3.56.0"},"reference-count":57,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T00:00:00Z","timestamp":1692662400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca (MIUR)","award":["AIM1892002"],"award-info":[{"award-number":["AIM1892002"]}]},{"name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca (MIUR)","award":["T3-AN-13-CUP D73C22000950001"],"award-info":[{"award-number":["T3-AN-13-CUP D73C22000950001"]}]},{"name":"Artificial Inteligence genoMic analysiS","award":["AIM1892002"],"award-info":[{"award-number":["AIM1892002"]}]},{"name":"Artificial Inteligence genoMic analysiS","award":["T3-AN-13-CUP D73C22000950001"],"award-info":[{"award-number":["T3-AN-13-CUP D73C22000950001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background: Advancements in DNA sequencing technology have facilitated the assessment of the connection between the oral microbiome and various diseases. The aim of the present study was to investigate the salivary microbiota composition employing for the first time in the literature the Oxford Nanopore Technology in patients affected by oral squamous cell carcinoma (OSCC). Methods: Unstimulated saliva samples of 31 patients were collected (24 OSCC patients and 7 controls). DNA was extracted using the QIAamp DNA Blood Kit and metagenomic long sequencing reads were performed using the MinION device. Results: In the OSCC group, 13 were males and 11 were females, with a mean age of 65.5 \u00b1 13.9 years; in the control group, 5 were males and 2 were females, with a mean age of 51.4 \u00b1 19.2 years. The border of the tongue was the most affected OSCC site. The microorganisms predominantly detected in OSCC patients were Prevotella, Chlamydia, Tissierellia, Calothrix, Leotiomycetes, Firmicutes and Zetaproteobacteria. Conclusions: This study confirmed the predominance of periodontopathic bacteria in the salivary microbiome in the OSCC group. If a direct correlation between oral dysbiosis and OSCC onset was proven, it could lead to new prevention strategies and early diagnostic tools.<\/jats:p>","DOI":"10.3390\/cancers15174211","type":"journal-article","created":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T08:01:21Z","timestamp":1692777681000},"page":"4211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["No Clear Clustering Dysbiosis from Salivary Microbiota Analysis by Long Sequencing Reads in Patients Affected by Oral Squamous Cell Carcinoma: A Single Center Study"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4008-6502","authenticated-orcid":false,"given":"Rodolfo","family":"Mauceri","sequence":"first","affiliation":[{"name":"Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy"},{"name":"Unit of Oral Medicine and Dentistry for Frail Patients, Department of Rehabilitation, Fragility and Continuity of Care, University Hospital Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0834-1841","authenticated-orcid":false,"given":"Martina","family":"Coppini","sequence":"additional","affiliation":[{"name":"Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy"},{"name":"Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, 90100 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3274-1746","authenticated-orcid":false,"given":"Davide","family":"Vacca","sequence":"additional","affiliation":[{"name":"Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy"},{"name":"Tumor Immunology Unit, Department of Sciences for Health Promotion and Mother-Child Care \u201cG. D\u2019Alessandro\u201d, University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio","family":"Bertolazzi","sequence":"additional","affiliation":[{"name":"Tumor Immunology Unit, Department of Sciences for Health Promotion and Mother-Child Care \u201cG. D\u2019Alessandro\u201d, University of Palermo, 90127 Palermo, Italy"},{"name":"Department of Economics, Business and Statistics, University of Palermo, 90128 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Cancila","sequence":"additional","affiliation":[{"name":"Tumor Immunology Unit, Department of Sciences for Health Promotion and Mother-Child Care \u201cG. D\u2019Alessandro\u201d, University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Tripodo","sequence":"additional","affiliation":[{"name":"Tumor Immunology Unit, Department of Sciences for Health Promotion and Mother-Child Care \u201cG. D\u2019Alessandro\u201d, University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9443-0495","authenticated-orcid":false,"given":"Giuseppina","family":"Campisi","sequence":"additional","affiliation":[{"name":"Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy"},{"name":"Unit of Oral Medicine and Dentistry for Frail Patients, Department of Rehabilitation, Fragility and Continuity of Care, University Hospital Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1038\/s41579-018-0089-x","article-title":"The Oral Microbiota: Dynamic Communities and Host Interactions","volume":"16","author":"Lamont","year":"2018","journal-title":"Nat. Rev. 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