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Patient-derived sarcoma organoids (PDSOs) have emerged as a promising tool in the multimodal management of sarcomas, offering unprecedented opportunities for personalized medicine and improved treatment strategies. This review aims to explore the potential of PDSOs as a promising tool for multimodal management of sarcomas. We discuss the establishment and characterization of PDSOs, which realistically recapitulate the complexity and heterogeneity of the original tumor, providing a platform for genetic and molecular fidelity, histological resemblance, and functional characterization. Additionally, we discuss the applications of PDSOs in pathological and genetic evaluation, treatment screening and development, and personalized multimodal management. One significant advancement of PDSOs lies in their ability to guide personalized treatment decisions, enabling clinicians to assess the response and efficacy of different therapies in a patient-specific manner. Through continued research and development, PDSOs hold the potential to revolutionize sarcoma management and drive advancements in personalized medicine, biomarker discovery, preclinical modeling, and therapy optimization. The integration of PDSOs into clinical practice can ultimately improve patient outcomes and significantly impact the field of sarcoma treatment.<\/jats:p>","DOI":"10.3390\/cancers15174339","type":"journal-article","created":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T10:09:49Z","timestamp":1693390189000},"page":"4339","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Patient-Derived Organoids as a Promising Tool for Multimodal Management of Sarcomas"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3547-1796","authenticated-orcid":false,"given":"Songfeng","family":"Xu","sequence":"first","affiliation":[{"name":"National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Shenzhen 518116, China"},{"name":"Department of Orthopedics, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3782-261X","authenticated-orcid":false,"given":"ShihJye","family":"Tan","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, Department of Biology, Academy for Advanced Interdisciplinary Studies, Southern University of Science and Technology, 1088 Xueyuan Blvd, Biology Building 402, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100021, China"},{"name":"Guangdong Provincial Key Laboratory of Cell Microenvironment and Disease Research, Department of Biology, Academy for Advanced Interdisciplinary Studies, Southern University of Science and Technology, 1088 Xueyuan Blvd, Biology Building 402, Shenzhen 518055, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1634\/theoncologist.2016-0079","article-title":"Sarcomas Associated With Genetic Cancer Predisposition Syndromes: A Review","volume":"21","author":"Farid","year":"2016","journal-title":"Oncologist"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3406","DOI":"10.1038\/s41467-022-30496-0","article-title":"Clinical genomic profiling in the management of patients with soft tissue and bone sarcoma","volume":"13","author":"Gounder","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1186\/s12885-020-07730-3","article-title":"Actual long-term survival after resection of stage III soft tissue sarcoma","volume":"21","author":"Lee","year":"2021","journal-title":"BMC Cancer"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1007\/s11999.0000000000000158","article-title":"What is the success of repeat surgical treatment of a local recurrence after initial wide resection of soft tissue sarcomas?","volume":"476","author":"Sugiura","year":"2018","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Psilopatis, I., Kokkali, S., Palamaris, K., Digklia, A., Vrettou, K., and Theocharis, S. (2022). Organoids: A New Chapter in Sarcoma Diagnosis and Treatment. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231911271"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/j.ctrv.2006.05.005","article-title":"Primary bone osteosarcoma in the pediatric age: State of the art","volume":"32","author":"Longhi","year":"2006","journal-title":"Cancer Treat. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.bbcan.2014.02.005","article-title":"Targeting receptor tyrosine kinases in osteosarcoma and Ewing sarcoma: Current hurdles and future perspectives","volume":"1845","author":"Fleuren","year":"2014","journal-title":"Biochim. Et Biophys. Acta (BBA)-Rev. Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1002\/pbc.22194","article-title":"Epidemiology of bone tumours in children and young adults","volume":"53","author":"Eyre","year":"2009","journal-title":"Pediatr. Blood Cancer"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e106","DOI":"10.1016\/j.jormas.2021.06.017","article-title":"Malignant fibrous histiocytoma of the floor of mouth: A case report and review of the literature","volume":"123","author":"Wu","year":"2022","journal-title":"J. Stomatol. Oral Maxillofac. Surg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1016\/S0090-4295(03)00021-9","article-title":"Clinical presentation and outcome of high-grade urinary bladder leiomyosarcoma in adults","volume":"61","author":"Rosser","year":"2003","journal-title":"Urology"},{"key":"ref_11","unstructured":"Souhami, R., and Tobias, J.S. (2008). Cancer and Its Management, John Wiley & Sons."},{"key":"ref_12","unstructured":"DeVita, V.T., Lawrence, T.S., and Rosenberg, S.A. (2008). DeVita, Hellman, and Rosenberg\u2019s Cancer: Principles & Practice of Oncology, Lippincott Williams & Wilkins."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1097\/PAS.0000000000001552","article-title":"The 2020 WHO Classification: What\u2019s new in soft tissue tumor pathology?","volume":"45","author":"Kallen","year":"2021","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1111\/imr.12766","article-title":"Immune checkpoint inhibitors: The linchpins of modern immunotherapy","volume":"290","author":"Wilky","year":"2019","journal-title":"Immunol. Rev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1002\/jso.25606","article-title":"Recurrence patterns after resection of retroperitoneal sarcomas: An eight-institution study from the US Sarcoma Collaborative","volume":"120","author":"Chouliaras","year":"2019","journal-title":"J. Surg. Oncol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1016\/S0959-8049(01)00083-1","article-title":"A randomised phase II study on neo-adjuvant chemotherapy for \u2018high-risk\u2019 adult soft-tissue sarcoma","volume":"37","author":"Gortzak","year":"2001","journal-title":"Eur. J. Cancer"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1227","DOI":"10.1002\/jso.25694","article-title":"Role of radiation therapy for retroperitoneal sarcomas: An eight-institution study from the US Sarcoma Collaborative","volume":"120","author":"Chouliaras","year":"2019","journal-title":"J. Surg. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1137736","DOI":"10.3389\/fendo.2023.1137736","article-title":"Editorial: New therapies in the treatment of sarcomas","volume":"14","author":"Gartland","year":"2023","journal-title":"Front. Endocrinol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/s41467-020-20603-4","article-title":"Sarcoma classification by DNA methylation profiling","volume":"12","author":"Koelsche","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1038\/nrc3087","article-title":"Advances in sarcoma genomics and new therapeutic targets","volume":"11","author":"Taylor","year":"2011","journal-title":"Nat. Rev. Cancer"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e001580","DOI":"10.1136\/jitc-2020-001580","article-title":"Immunotherapy for sarcomas: New frontiers and unveiled opportunities","volume":"9","author":"Birdi","year":"2021","journal-title":"J. Immunother. Cancer"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1038\/s41586-019-1906-8","article-title":"B cells are associated with survival and immunotherapy response in sarcoma","volume":"577","author":"Petitprez","year":"2020","journal-title":"Nature"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1038\/s41580-020-0259-3","article-title":"Human organoids: Model systems for human biology and medicine","volume":"21","author":"Kim","year":"2020","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Vivarelli, S., Candido, S., Caruso, G., Falzone, L., and Libra, M. (2020). Patient-Derived Tumor Organoids for Drug Repositioning in Cancer Care: A Promising Approach in the Era of Tailored Treatment. Cancers, 12.","DOI":"10.3390\/cancers12123636"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"7354","DOI":"10.1245\/s10434-022-12086-y","article-title":"Patient-Specific Sarcoma Organoids for Personalized Translational Research: Unification of the Operating Room with Rare Cancer Research and Clinical Implications","volume":"29","author":"Forsythe","year":"2022","journal-title":"Ann. Surg. Oncol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/s41698-021-00168-1","article-title":"Patient-derived organoids as a predictive biomarker for treatment response in cancer patients","volume":"5","author":"Wensink","year":"2021","journal-title":"Npj Precis. Oncol."},{"key":"ref_27","unstructured":"Smith, A.P., Dueber, J.C., and Allison, D.B. (2020). Seminars in Diagnostic Pathology, Elsevier."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S1470-2045(00)00016-4","article-title":"Management of soft-tissue sarcomas: An overview and update","volume":"1","author":"Singer","year":"2000","journal-title":"Lancet Oncol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"100039","DOI":"10.1016\/j.labinv.2022.100039","article-title":"Addressing Modern Diagnostic Pathology for Patient-Derived Soft Tissue Sarcosphere Models in the Era of Functional Precision Oncology","volume":"103","author":"Chen","year":"2023","journal-title":"Lab. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1007\/s13577-022-00818-x","article-title":"Establishment, characterization and functional testing of two novel ex vivo extraskeletal myxoid chondrosarcoma (EMC) cell models","volume":"36","author":"Bangerter","year":"2022","journal-title":"Hum. Cell"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e202000808","DOI":"10.26508\/lsa.202000808","article-title":"The chromatin landscape of primary synovial sarcoma organoids is linked to specific epigenetic mechanisms and dependencies","volume":"4","author":"Boulay","year":"2020","journal-title":"Life Sci. Alliance"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"893592","DOI":"10.3389\/fonc.2022.893592","article-title":"Establishment of Organoids From Human Epithelioid Sarcoma With the Air-Liquid Interface Organoid Cultures","volume":"12","author":"Wakamatsu","year":"2022","journal-title":"Front. Oncol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e16001","DOI":"10.15252\/emmm.202216001","article-title":"Mesenchymal tumor organoid models recapitulate rhabdomyosarcoma subtypes","volume":"14","author":"Meister","year":"2022","journal-title":"EMBO Mol. Med."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Komatsu, A., Matsumoto, K., Yoshimatsu, Y., Sin, Y., Kubota, A., Saito, T., Mizumoto, A., Ohashi, S., Muto, M., and Noguchi, R. (2021). The CAM Model for CIC-DUX4 Sarcoma and Its Potential Use for Precision Medicine. Cells, 10.","DOI":"10.3390\/cells10102613"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Maloney, E., Clark, C., Sivakumar, H., Yoo, K., Aleman, J., Rajan, S.A.P., Forsythe, S., Mazzocchi, A., Laxton, A.W., and Tatter, S.B. (2020). Immersion Bioprinting of Tumor Organoids in Multi-Well Plates for Increasing Chemotherapy Screening Throughput. Micromachines, 11.","DOI":"10.3390\/mi11020208"},{"key":"ref_36","unstructured":"MEMd, C., Zaidi, S., Scoazec, J., Droit, R., Lim, W.C., Marchais, A., Salmon, J., Cherkaoui, S., Morscher, R., and Laurent, A. (2023, August 16). Pediatric Patient-Derived-Xenograft Development in MAPPYACTS\u2013International Pediatric Cancer Precision Medicine Trial in Relapsed and Refractory Tumors. Available online: https:\/\/europepmc.org\/article\/ppr\/ppr620267."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"e48","DOI":"10.1016\/j.clon.2022.06.002","article-title":"A Multicentre Clinical Study of Sarcoma Personalised Treatment Using Patient-Derived Tumour Xenografts","volume":"35","author":"Xu","year":"2023","journal-title":"Clin. Oncol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Colella, G., Fazioli, F., Gallo, M., De Chiara, A., Apice, G., Ruosi, C., Cimmino, A., and de Nigris, F. (2018). Sarcoma Spheroids and Organoids\u2014Promising Tools in the Era of Personalized Medicine. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19020615"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Nami, B., and Wang, Z. (2018). Genetics and Expression Profile of the Tubulin Gene Superfamily in Breast Cancer Subtypes and Its Relation to Taxane Resistance. Cancers, 10.","DOI":"10.3390\/cancers10080274"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1247125","DOI":"10.1126\/science.1247125","article-title":"Organogenesis in a dish: Modeling development and disease using organoid technologies","volume":"345","author":"Lancaster","year":"2014","journal-title":"Science"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1186\/s13045-020-00931-0","article-title":"Modeling neoplastic disease with spheroids and organoids","volume":"13","author":"Zanoni","year":"2020","journal-title":"J. Hematol. Oncol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1016\/j.cell.2019.04.004","article-title":"Visualizing Engrafted Human Cancer and Therapy Responses in Immunodeficient Zebrafish","volume":"177","author":"Yan","year":"2019","journal-title":"Cell"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1016\/j.cell.2016.08.041","article-title":"A Biobank of Breast Cancer Explants with Preserved Intra-tumor Heterogeneity to Screen Anticancer Compounds","volume":"167","author":"Bruna","year":"2016","journal-title":"Cell"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1038\/nature23647","article-title":"Orthotopic patient-derived xenografts of paediatric solid tumours","volume":"549","author":"Stewart","year":"2017","journal-title":"Nature"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1038\/s41588-020-00750-6","article-title":"Conservation of copy number profiles during engraftment and passaging of patient-derived cancer xenografts","volume":"53","author":"Woo","year":"2021","journal-title":"Nat. Genet."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s00401-020-02171-5","article-title":"Patient-derived orthotopic xenografts of pediatric brain tumors: A St. Jude resource","volume":"140","author":"Smith","year":"2020","journal-title":"Acta Neuropathol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.1016\/j.cell.2019.02.012","article-title":"Characterizing Mutational Signatures in Human Cancer Cell Lines Reveals Episodic APOBEC Mutagenesis","volume":"176","author":"Petljak","year":"2019","journal-title":"Cell"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1186\/s13046-016-0462-4","article-title":"Patient-derived xenografts: A relevant preclinical model for drug development","volume":"35","author":"Pompili","year":"2016","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"998","DOI":"10.1158\/2159-8290.CD-14-0001","article-title":"Patient-Derived Xenograft Models: An Emerging Platform for Translational Cancer Research","volume":"4","author":"Hidalgo","year":"2014","journal-title":"Cancer Discov."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"100486","DOI":"10.1016\/j.jbo.2023.100486","article-title":"Genetic characterization of a novel organoid from human malignant giant-cell tumor","volume":"41","author":"Suzuki","year":"2023","journal-title":"J. Bone Oncol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"183","DOI":"10.3389\/fonc.2013.00183","article-title":"Human Rhabdomyosarcoma Cell Lines for Rhabdomyosarcoma Research: Utility and Pitfalls","volume":"3","author":"Hinson","year":"2013","journal-title":"Front. Oncol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Vibert, J., and Watson, S. (2022). The Molecular Biology of Soft Tissue Sarcomas: Current Knowledge and Future Perspectives. Cancers, 14.","DOI":"10.3390\/cancers14102548"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.1038\/s41467-022-30710-z","article-title":"The androgen receptor is a therapeutic target in desmoplastic small round cell sarcoma","volume":"13","author":"Maitituoheti","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1038\/s41563-020-0754-0","article-title":"Somatic cell-derived organoids as prototypes of human epithelial tissues and diseases","volume":"20","author":"Fujii","year":"2020","journal-title":"Nat. Mater."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e274","DOI":"10.1002\/mco2.274","article-title":"Organoids: The current status and biomedical applications","volume":"4","author":"Yang","year":"2023","journal-title":"Medcomm"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"7239","DOI":"10.1245\/s10434-022-12152-5","article-title":"Patient-Derived Sarcoma Organoids Offer a Novel Platform for Personalized Precision Medicine","volume":"29","author":"Sharma","year":"2022","journal-title":"Ann. Surg. Oncol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1186\/s13045-022-01278-4","article-title":"Tumor organoids: Applications in cancer modeling and potentials in precision medicine","volume":"15","author":"Xu","year":"2022","journal-title":"J. Hematol. Oncol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"763640","DOI":"10.3389\/fcell.2021.763640","article-title":"The Extracellular Matrix in Soft Tissue Sarcomas: Pathobiology and Cellular Signalling","volume":"9","author":"Pankova","year":"2021","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1007\/s00330-008-1292-3","article-title":"Soft tissue sarcomas at a glance: Clinical, histological, and MR imaging features of malignant extremity soft tissue tumors","volume":"19","author":"Kliffen","year":"2009","journal-title":"Eur. Radiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1089\/ten.teb.2019.0302","article-title":"Modeling the Tumor Microenvironment and Pathogenic Signaling in Bone Sarcoma","volume":"26","author":"Molina","year":"2020","journal-title":"Tissue Eng. Part B Rev."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Rae, C., Amato, F., and Braconi, C. (2021). Patient-Derived Organoids as a Model for Cancer Drug Discovery. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22073483"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1158\/2767-9764.CRC-21-0047","article-title":"BRCA1-Associated RING Domain-1 (BARD1) Loss and GBP1 Expression Enhance Sensitivity to DNA Damage in Ewing Sarcoma","volume":"2","author":"Maurer","year":"2022","journal-title":"Cancer Res. Commun."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s12032-020-01429-y","article-title":"Organoid culture system for patient-derived lung metastatic osteosarcoma","volume":"37","author":"He","year":"2020","journal-title":"Med. Oncol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1158\/1535-7163.MCT-15-0867","article-title":"Dual Carfilzomib and Doxorubicin\u2013Loaded Liposomal Nanoparticles for Synergistic Efficacy in Multiple Myeloma","volume":"15","author":"Ashley","year":"2016","journal-title":"Mol. Cancer Ther."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3776","DOI":"10.1158\/1078-0432.CCR-18-1909","article-title":"A Phase I\/II Trial of Carfilzomib, Pegylated Liposomal Doxorubicin, and Dexamethasone for the Treatment of Relapsed\/Refractory Multiple MyelomaKDD for RRMM","volume":"25","author":"Schroeder","year":"2019","journal-title":"Clin. Cancer Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1158\/1538-7445.AM2019-469","article-title":"Abstract 469: Patient-derived sarcoma models: First results from the SARQMA study","volume":"79","author":"Gaebler","year":"2019","journal-title":"Cancer Res."},{"key":"ref_67","unstructured":"Johansson, S. (2023, August 16). Patient-derived Organoid Culture for 3D Culture of Colorectal Cancer, Renal Cancer and Osteosarcoma. Available online: https:\/\/www.diva-portal.org\/smash\/get\/diva2:1344438\/FULLTEXT01.pdf."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Veys, C., Benmoussa, A., Contentin, R., Duchemin, A., Brotin, E., Lafont, J.E., Saintigny, Y., Poulain, L., Denoyelle, C., and Demoor, M. (2021). Tumor Suppressive Role of miR-342-5p in Human Chondrosarcoma Cells and 3D Organoids. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22115590"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"6887","DOI":"10.2147\/OTT.S220045","article-title":"Recent advances and application of PD-1 blockade in sarcoma","volume":"12","author":"Zuo","year":"2019","journal-title":"OncoTargets Ther."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/S1470-2045(18)30006-8","article-title":"Nivolumab with or without ipilimumab treatment for metastatic sarcoma (Alliance A091401): Two open-label, non-comparative, randomised, phase 2 trials","volume":"19","author":"Mahoney","year":"2018","journal-title":"Lancet Oncol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1038\/s41577-019-0210-z","article-title":"Dendritic cells in cancer immunology and immunotherapy","volume":"20","author":"Wculek","year":"2020","journal-title":"Nat. Rev. Immunol."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/s41422-020-0337-2","article-title":"The future of cancer immunotherapy: Microenvironment-targeting combinations","volume":"30","author":"Warner","year":"2020","journal-title":"Cell Res."}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/15\/17\/4339\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:43:05Z","timestamp":1760128985000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/15\/17\/4339"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,30]]},"references-count":72,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["cancers15174339"],"URL":"https:\/\/doi.org\/10.3390\/cancers15174339","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,30]]}}}