{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T04:13:34Z","timestamp":1784175214400,"version":"3.55.0"},"reference-count":47,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T00:00:00Z","timestamp":1694736000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea","award":["2020R1F1A1070784"],"award-info":[{"award-number":["2020R1F1A1070784"]}]},{"name":"National Research Foundation of Korea","award":["2021R1A2C1011864"],"award-info":[{"award-number":["2021R1A2C1011864"]}]},{"name":"National Research Foundation of Korea","award":["2014M3A9D5A0107512833"],"award-info":[{"award-number":["2014M3A9D5A0107512833"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Recent studies have shown that mutations in members of the G-protein \u03b1 family contribute to the onset and progression of cancer. However, the role of GNA14 in CRC remains unknown. In this study, we examined the effect of GNA14 on CRC through genetic approaches in vitro and in vivo. We found that GNA14 knockdown by small interfering RNA (siRNA) inhibited the proliferation of CRC cells SW403 and HT29. Gna14 knockout mice developed normally without obvious abnormalities. However, the number of polyps in the small intestine was significantly reduced in Gna14 knockout mice compared to control mice after mating with ApcMin mice, a representative CRC mouse model. In particular, deletion of the Gna14 inhibited polyp growth, especially in the distal end of the small intestine. Histological examination showed that Gna14 knockout mice suppressed malignant tumor progression due to decreased proliferation and increased apoptosis in polyps compared to controls. In addition, GNA14 knockdown in CRC cells resulted in downregulation of ERK phosphorylation and \u03b2-catenin and \u03b2-catenin phosphorylation at S675. Similarly, ERK phosphorylation and phospho-\u03b2-catenin phosphorylation at S675 were decreased in polyps of Gna14 knockout mice. Collectively, these analyses show that GNA14 may accelerate CRC cell proliferation and malignant tumor progression through ERK and \u03b2-catenin pathways.<\/jats:p>","DOI":"10.3390\/cancers15184572","type":"journal-article","created":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T04:06:13Z","timestamp":1694750773000},"page":"4572","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Tumor-Promoting Role of GNA14 in Colon Cancer Development"],"prefix":"10.3390","volume":"15","author":[{"given":"Rahui","family":"Park","sequence":"first","affiliation":[{"name":"Department of Life Science, Ewha Womans University, Seoul 03760, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seungmin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Science, Ewha Womans University, Seoul 03760, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyunjung","family":"Chin","sequence":"additional","affiliation":[{"name":"Department of Life Science, Ewha Womans University, Seoul 03760, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anh Thai-Quynh","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Life Science, Ewha Womans University, Seoul 03760, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daekee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Life Science, Ewha Womans University, Seoul 03760, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1038\/sj.onc.1202186","article-title":"Cell growth control by G protein-coupled receptors: From signal transduction to signal integration","volume":"17","author":"Gutkind","year":"1998","journal-title":"Oncogene"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/nrc2069","article-title":"G-protein-coupled receptors and cancer","volume":"7","author":"Dorsam","year":"2007","journal-title":"Nat. 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