{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:59:44Z","timestamp":1787165984664,"version":"build-2736575974"},"reference-count":52,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T00:00:00Z","timestamp":1695772800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agence Nationale de Recherche Scientifique (ANRS)"},{"name":"Fondation Bettencourt Schueller (FBS)"},{"name":"Association Institut du Cancer et d\u2019Immunog\u00e9n\u00e9tique (ICIG)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Tspan8 is a member of the tetraspanins family of cell surface molecules. The ability of tetraspanins to organize membrane microdomains with other membrane molecules and interfere with their function suggests that they could act as surface integrators of external or internal signals. Among the first identified tetraspanins, Tspan8 promotes tumor progression and metastasis, presumably by stimulating angiogenesis and cell motility. In patients, its expression on digestive tract tumors seems to be associated with a bad prognosis. We showed previously that Tspan8 associates with E-cadherin and EGFR and modulates their effects on cell motility. Using Mass spectrometry and western blot, we found a new partner, the endothelin converting enzyme ECE1, and showed that Tspan8 amplifies its activity of conversion of the endothelin-1 precursor bigET1 to endothelin. This was observed by transduction of the colon carcinoma cell line Isreco1, which does not express Tspan8, and on ileum tissue fragments of tspan8ko mice versus wild type mice. Given these results, Tspan8 appears to be a modulator of the endothelin axis, which could possibly be targeted in case of over-activity of endothelins in biological processes of tissues expressing Tspan8.<\/jats:p>","DOI":"10.3390\/cancers15194751","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T07:38:46Z","timestamp":1695800326000},"page":"4751","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Tetraspanin Tspan8 Associates with Endothelin Converting Enzyme ECE1 and Regulates Its Activity"],"prefix":"10.3390","volume":"15","author":[{"given":"Yingying","family":"Zhu","sequence":"first","affiliation":[{"name":"UMR-S 1004, SFR Andr\u00e9 Lwoff, Inserm, 94800 Villejuif, France"},{"name":"Universit\u00e9 Paris Saclay, 91190 Gif-sur-Yvette, France"},{"name":"Department of Oncology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430074, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2676-0791","authenticated-orcid":false,"given":"Julien","family":"Saint-Pol","sequence":"additional","affiliation":[{"name":"UMR-S 1004, SFR Andr\u00e9 Lwoff, Inserm, 94800 Villejuif, France"},{"name":"Universit\u00e9 Artois, UR 2465, Blood-Brain Barrier Laboratory (LBHE), 62300 Lens, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viet","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Saclay, 91190 Gif-sur-Yvette, France"},{"name":"Plateforme Spectrom\u00e9trie de Masse, Laboratoire Biochimie-Hormonologie, H\u00f4pital Robert-Debr\u00e9, Assistance Publique H\u00f4pitaux de Paris (APHP), 75610 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7623-9665","authenticated-orcid":false,"given":"Eric","family":"Rubinstein","sequence":"additional","affiliation":[{"name":"UMR-S 1004, SFR Andr\u00e9 Lwoff, Inserm, 94800 Villejuif, France"},{"name":"Centre d\u2019Immunologie et des Maladies Infectieuses, Inserm, CNRS, Sorbonne Universit\u00e9, CIMI-Paris, 75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4184-7008","authenticated-orcid":false,"given":"Claude","family":"Boucheix","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Saclay, 91190 Gif-sur-Yvette, France"},{"name":"UMR-S 1197, SFR Andr\u00e9 Lwoff, Inserm, 94800 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9line","family":"Greco","sequence":"additional","affiliation":[{"name":"Department of Pain and Palliative Care, H\u00f4pital Necker-Enfants Malades, Assistance Publique H\u00f4pitaux de Paris (APHP), 75610 Paris, France"},{"name":"U1163, IMAGINE Institute, Universit\u00e9 de Paris Cit\u00e9, Inserm, 75014 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"6833","DOI":"10.1073\/pnas.87.17.6833","article-title":"Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens","volume":"87","author":"Szala","year":"1990","journal-title":"Proc. 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