{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:04:48Z","timestamp":1785413088399,"version":"3.56.0"},"reference-count":52,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T00:00:00Z","timestamp":1695859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Erling Persson Foundation","award":["2019.0341"],"award-info":[{"award-number":["2019.0341"]}]},{"name":"Knut and Alice Wallenberg Foundation","award":["2019.0341"],"award-info":[{"award-number":["2019.0341"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Mass spectrometry based on data-independent acquisition (DIA) has developed into a powerful quantitative tool with a variety of implications, including precision medicine. Combined with stable isotope recombinant protein standards, this strategy provides confident protein identification and precise quantification on an absolute scale. Here, we describe a comprehensive targeted proteomics approach to profile a pan-cancer cohort consisting of 1800 blood plasma samples representing 15 different cancer types. We successfully performed an absolute quantification of 253 proteins in multiplex. The assay had low intra-assay variability with a coefficient of variation below 20% (CV = 17.2%) for a total of 1013 peptides quantified across almost two thousand injections. This study identified a potential biomarker panel of seven protein targets for the diagnosis of multiple myeloma patients using differential expression analysis and machine learning. The combination of markers, including the complement C1 complex, JCHAIN, and CD5L, resulted in a prediction model with an AUC of 0.96 for the identification of multiple myeloma patients across various cancer patients. All these proteins are known to interact with immunoglobulins.<\/jats:p>","DOI":"10.3390\/cancers15194764","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T01:51:14Z","timestamp":1695865874000},"page":"4764","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Absolute Quantification of Pan-Cancer Plasma Proteomes Reveals Unique Signature in Multiple Myeloma"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5388-3826","authenticated-orcid":false,"given":"David","family":"Kotol","sequence":"first","affiliation":[{"name":"Science For Life Laboratory, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"},{"name":"Department of Protein Science, Division of Systems Biology, School of Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2283-7237","authenticated-orcid":false,"given":"Jakob","family":"Woessmann","sequence":"additional","affiliation":[{"name":"Science For Life Laboratory, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"},{"name":"Department of Protein Science, Division of Systems Biology, School of Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Hober","sequence":"additional","affiliation":[{"name":"Science For Life Laboratory, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"},{"name":"Department of Protein Science, Division of Systems Biology, School of Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2669-7796","authenticated-orcid":false,"given":"Mar\u00eda Bueno","family":"\u00c1lvez","sequence":"additional","affiliation":[{"name":"Science For Life Laboratory, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"},{"name":"Department of Protein Science, Division of Systems Biology, School of Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khue Hua","family":"Tran Minh","sequence":"additional","affiliation":[{"name":"Science For Life Laboratory, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"},{"name":"Department of Protein Science, Division of Systems Biology, School of Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredrik","family":"Pont\u00e9n","sequence":"additional","affiliation":[{"name":"Rudbeck Laboratory, Uppsala University, 752 36 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0198-7137","authenticated-orcid":false,"given":"Linn","family":"Fagerberg","sequence":"additional","affiliation":[{"name":"Science For Life Laboratory, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"},{"name":"Department of Protein Science, Division of Systems Biology, School of Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Uhl\u00e9n","sequence":"additional","affiliation":[{"name":"Science For Life Laboratory, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"},{"name":"Department of Protein Science, Division of Systems Biology, School of Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0017-7987","authenticated-orcid":false,"given":"Fredrik","family":"Edfors","sequence":"additional","affiliation":[{"name":"Science For Life Laboratory, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"},{"name":"Department of Protein Science, Division of Systems Biology, School of Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, 114 28 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/ng1060","article-title":"A molecular signature of metastasis in primary solid tumors","volume":"33","author":"Ramaswamy","year":"2003","journal-title":"Nat. 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