{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T16:35:41Z","timestamp":1782232541521,"version":"3.54.5"},"reference-count":60,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:00:00Z","timestamp":1697414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tempus Public Foundation"},{"name":"University of P\u1ebbcs"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Colon tumors have a very complicated and poorly understood pathogenesis. Plant-based organic compounds might provide a novel source for cancer treatment with a sufficient novel mode of action. The objective of this study was to analyze and evaluate the efficacy of Aloe secundiflora\u2019s (AS) methanolic extracts on the expression of CASPS9, 5-LOX, Bcl2, Bcl-xL, and COX-2 in colorectal cancer (CRC) management. Caco-2 cell lines were used in the experimental study. In the serial exhaustive extraction (SEE) method, methanol was utilized as the extraction solvent. Upon treatment of CASPS9 with the methanolic extracts, the expression of the genes was progressively upregulated, thus, dose-dependently increasing the rate of apoptosis. On the other hand, the expressions of 5-LOX, Bcl2, and Bcl-xL were variably downregulated in a dose-dependent manner. This is a unique novel study that evaluated the effects of AS methanolic extracts in vitro on CRC cell lines using different dosage concentrations. We, therefore, recommend the utilization of AS and the application of methanol as the extraction solvent of choice for maximum modulatory benefits in CRC management. In addition, we suggest research on the specific metabolites in AS involved in the modulatory pathways that suppress the development of CRC and potential metastases.<\/jats:p>","DOI":"10.3390\/cancers15205002","type":"journal-article","created":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T05:43:49Z","timestamp":1697435029000},"page":"5002","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Modulatory Properties of Aloe secundiflora\u2019s Methanolic Extracts on Targeted Genes in Colorectal Cancer Management"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7535-9478","authenticated-orcid":false,"given":"John M.","family":"Macharia","sequence":"first","affiliation":[{"name":"Doctoral School of Health Sciences, Faculty of Health Science, University of P\u1ebbcs, 7621 P\u1ebbcs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4950-0669","authenticated-orcid":false,"given":"Timea","family":"Varjas","sequence":"additional","affiliation":[{"name":"Department of Public Health Medicine, Medical School, University of P\u1ebbcs, 7621 P\u1ebbcs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth W.","family":"Mwangi","sequence":"additional","affiliation":[{"name":"Department of Vegetable and Mushroom Growing, Hungarian University of Agriculture and Life Sciences, 1118 Budapest, Hungary"},{"name":"Department of Biological Sciences, Egerton University, Nakuru P.O. Box 3366-20100, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsolt","family":"K\u00e1poszt\u00e1s","sequence":"additional","affiliation":[{"name":"Faculty of Health Sciences, University of P\u00e9cs, 7621 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00f3ra","family":"Rozmann","sequence":"additional","affiliation":[{"name":"Doctoral School of Health Sciences, Faculty of Health Science, University of P\u1ebbcs, 7621 P\u1ebbcs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rton","family":"Pint\u00e9r","sequence":"additional","affiliation":[{"name":"Doctoral School of Health Sciences, Faculty of Health Science, University of P\u1ebbcs, 7621 P\u1ebbcs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel N.","family":"Wagara","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Egerton University, Nakuru P.O. 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