{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T18:37:32Z","timestamp":1766428652951,"version":"build-2065373602"},"reference-count":53,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:00:00Z","timestamp":1705536000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Faculty of Dentistry, University of Oslo (DS), the Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo (FAC)"},{"name":"Department of Medical Biochemistry, Oslo University Hospital (TPU)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>The aerobic glycolytic pathway, boosting lactate formation, and glutamine addiction are two hallmarks of cancer pathophysiology. Consistent with this, several cell membrane glutamine transporters, belonging to different solute carrier (SLC) families, have been shown to be upregulated in a cell-specific manner to furnish the cells with glutamine and glutamine-derived metabolic intermediates. Among them, the system A transporter Slc38a1 has a higher affinity for glutamine compared to other SLC transporters, and it undergoes highly multifaceted regulation at gene and protein levels. The current study aimed to investigate the functional role of Slc38a1 in the proliferation and maturation of the mouse tongue epithelium. Secondly, we aimed to examine the expression of SLC38A1 and its regulation in human tongue oral squamous cell carcinoma (OTSCC). Employing Slc38a1 wild-type and knockout mice, we showed that Slc38a1 was not directly linked to the regulation of the proliferation and differentiation of the mouse tongue epithelium. External transcriptomic datasets and Western blot analyses showed upregulation of SLC38A1 mRNA\/protein in human OTSCC and oral cancer cell lines as compared to the corresponding controls. Further, an investigation of external datasets indicated that mechanisms other than the amplification of the SLC38A1 chromosomal locus or hypomethylation of the SLC38A1 promoter region might be important for the upregulation of SLC38A1 in OTSCC.<\/jats:p>","DOI":"10.3390\/cancers16020405","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T03:50:56Z","timestamp":1705549856000},"page":"405","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Investigation of Roles of SLC38A1 in Proliferation and Differentiation of Mouse Tongue Epithelium and Expression in Human Oral Tongue Squamous Cell Carcinoma"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0061-825X","authenticated-orcid":false,"given":"Dipak","family":"Sapkota","sequence":"first","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, University of Oslo, 0372 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, 0372 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olaf","family":"Schreurs","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, University of Oslo, 0372 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4852-8608","authenticated-orcid":false,"given":"Evan M.","family":"Vallenari","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, University of Oslo, 0372 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sushma","family":"Pandey Dhakal","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, University of Oslo, 0372 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3074-9509","authenticated-orcid":false,"given":"Thomas","family":"K\u00fcntziger","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, University of Oslo, 0372 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1507-4662","authenticated-orcid":false,"given":"Burcu Seng\u00fcven","family":"Tok\u00f6zl\u00fc","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, University of Oslo, 0372 Oslo, Norway"},{"name":"Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara 06510, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tor Paaske","family":"Utheim","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, University of Oslo, 0372 Oslo, Norway"},{"name":"Department of Plastic and Reconstructive Surgery, Oslo University Hospital, 0372 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2039-9667","authenticated-orcid":false,"given":"Farrukh Abbas","family":"Chaudhry","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, 0372 Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1083\/jcb.200201070","article-title":"The glutamine commute: Take the N line and transfer to the A","volume":"157","author":"Chaudhry","year":"2002","journal-title":"J. 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