{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T08:35:20Z","timestamp":1768552520466,"version":"3.49.0"},"reference-count":41,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T00:00:00Z","timestamp":1713744000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Armauer Hansen research Institute"},{"name":"Addis Ababa University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>SARS-CoV-2 and blood-borne viral coinfections are well reported. Nevertheless, little is known regarding the seroprevalence of SARS-CoV-2 and coinfection with blood-borne viruses in hematologic malignancy patients in Ethiopia. We aimed to assess the seroprevalence of SARS-CoV-2 and associated infections with hepatitis B and other viruses among adolescent and adult acute leukemia patients at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia. A cross-sectional study was conducted from July 2020 to June 2021. Blood samples were tested for the presence of anti-SARS-CoV-2, HBV, HCV, and HIV with ELISA kits and occult hepatitis B infection with a real-time polymerase chain reaction assay. Out of a total 110 cases, the SARS-CoV-2 seroprevalence was 35.5%. The prevalence showed a significant increment from July 2020 to the end of June 2021 (p = 0.015). In 22.7% and 2.7% of leukemia cases, HBV and HIV, respectively, were detected. No HCV was identified. The rate of SARS-CoV-2 coinfection with HBV and HIV was 28% (11\/39) and 2.6% (1\/39), respectively; however, there was no statistically significant association between SARS-CoV-2 seropositivity with HBV and HIV (p &gt; 0.05). There is a need for viral screening in leukemia cases to monitor infections and inform management.<\/jats:p>","DOI":"10.3390\/cancers16081606","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T07:44:09Z","timestamp":1713771849000},"page":"1606","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Seroprevalence of SARS-CoV-2 and Hepatitis B Virus Coinfections among Ethiopians with Acute Leukemia"],"prefix":"10.3390","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3850-7328","authenticated-orcid":false,"given":"Jemal","family":"Alemu","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"},{"name":"Armauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia"},{"name":"Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Balako","family":"Gumi","sequence":"additional","affiliation":[{"name":"Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aster","family":"Tsegaye","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziyada","family":"Rahimeto","sequence":"additional","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dessalegn","family":"Fentahun","sequence":"additional","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fozia","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulaziz","family":"Abubeker","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amha","family":"Gebremedhin","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa P.O. Box 1176, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0696-3786","authenticated-orcid":false,"given":"Tesfaye","family":"Gelanew","sequence":"additional","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rawleigh","family":"Howe","sequence":"additional","affiliation":[{"name":"Armauer Hansen Research Institute, Addis Ababa P.O. 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Infect."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"100742","DOI":"10.1016\/j.jbior.2020.100742","article-title":"The clinical impact of COVID-19 epidemic in the hematologic setting","volume":"77","author":"Finelli","year":"2020","journal-title":"Adv. Biol. Regul."},{"key":"ref_5","first-page":"1775","article-title":"Case-fatality rate and characteristics of patients dying in relation to COVID-19 in Italy","volume":"323","author":"Onder","year":"2020","journal-title":"JAMA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/bjh.16620","article-title":"Care of haematology patients in a COVID-19 epidemic","volume":"189","author":"Willan","year":"2020","journal-title":"Br. J. Haematol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.tmrv.2020.02.003","article-title":"Coronavirus disease 2019: Coronaviruses and blood safety","volume":"34","author":"Chang","year":"2020","journal-title":"Transfus. Med. Rev."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1038\/s41575-023-00760-9","article-title":"Global burden of hepatitis B virus: Current status, missed opportunities and a call for action","volume":"20","author":"Hsu","year":"2023","journal-title":"Nat. Rev. Gastroenterol. Hepatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41577-021-00549-4","article-title":"Immunobiology and pathogenesis of hepatitis B virus infection","volume":"22","author":"Iannacone","year":"2022","journal-title":"Nat. Rev. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1007\/s40121-022-00638-4","article-title":"Patients with COVID-19 and HBV Coinfection are at Risk of Poor Prognosis","volume":"11","author":"Yang","year":"2022","journal-title":"Infect. Dis. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"100167","DOI":"10.1016\/j.jcvp.2023.100167","article-title":"SARS-CoV-2 and chronic hepatitis B: Focusing on the possible consequences of co-infection","volume":"3","author":"Shoraka","year":"2023","journal-title":"J. Clin. Virol. Plus"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1016\/j.gendis.2020.11.005","article-title":"Patients with SARS-CoV-2 and HBV co-infection are at risk of greater liver injury","volume":"8","author":"Lin","year":"2021","journal-title":"Genes Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1001\/jamaoncol.2018.6437","article-title":"Prevalence of hepatitis B virus, hepatitis C virus, and HIV infection among patients with newly diagnosed cancer from academic and community oncology practices","volume":"5","author":"Ramsey","year":"2019","journal-title":"JAMA Oncol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1016\/S1473-3099(16)00118-3","article-title":"Management of viral hepatitis in patients with haematological malignancy and in patients undergoing haemopoietic stem cell transplantation: Recommendations of the 5th European Conference on Infections in Leukaemia (ECIL-5)","volume":"16","author":"Mallet","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_15","unstructured":"World Health Organization (WHO Africa) (2023, June 24). First Case of COVID-19 Confirmed in Ethiopia. Available online: https:\/\/www.afro.who.int\/news\/first-case-covid-19-confirmed-ethiopia#:~:text=March%2013%2C%202020%2C%20The%20Federal,in%20China%20in%20December%202019."},{"key":"ref_16","unstructured":"WHO (2023, December 19). COVID-19 Update in Ethiopia. Available online: https:\/\/covid19.who.int\/region\/afro\/country\/et."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"102102","DOI":"10.1016\/j.ctrv.2020.102102","article-title":"Seroprevalence of SARS-CoV-2\u2013specific antibodies in cancer outpatients in Madrid (Spain): A single center, prospective, cohort study and a review of available data","volume":"90","year":"2020","journal-title":"Cancer Treat. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1038\/s41375-020-0836-7","article-title":"COVID-19 in persons with haematological cancers","volume":"34","author":"He","year":"2020","journal-title":"Leukemia"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"106353","DOI":"10.1016\/j.leukres.2020.106353","article-title":"Acute leukemia in the time of COVID-19","volume":"92","author":"Gavillet","year":"2020","journal-title":"Leuk. Res."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Gelanew, T., Seyoum, B., Mulu, A., Mihret, A., Abebe, M., Wassie, L., Gelaw, B., Sorsa, A., Merid, Y., and Muchie, Y. (2022). High seroprevalence of anti-SARS-CoV-2 antibodies among Ethiopian healthcare workers. BMC Infect. Dis., 22.","DOI":"10.1186\/s12879-022-07247-z"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/s13027-023-00519-6","article-title":"Frequency of viral infections in adolescent and adult in-patient Ethiopians with acute leukemia at presentation to a tertiary care teaching hospital: A cross-sectional study","volume":"18","author":"Alemu","year":"2023","journal-title":"Infect. Agents Cancer"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"20503121221076931","DOI":"10.1177\/20503121221076931","article-title":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG-antibody seroprevalence among quarantined population, during the first wave of COVID-19 pandemic, In North West Ethiopia (from 30 April to 30 May 2020)","volume":"10","author":"Kebede","year":"2022","journal-title":"SAGE Open Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"20503121211062793","DOI":"10.1177\/20503121211062793","article-title":"Features associated with SARS-COV-2 positivity among people presenting with acute respiratory tract infections to public Hospitals in Harari region, Ethiopia","volume":"9","author":"Birhanu","year":"2021","journal-title":"SAGE Open Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2022","DOI":"10.4269\/ajtmh.20-0816","article-title":"SARS-CoV-2 serosurvey in Addis Ababa, Ethiopia","volume":"103","author":"Kempen","year":"2020","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.ijid.2021.08.028","article-title":"Seroprevalence and risk factors for SARS-CoV-2 Infection in selected urban areas in Ethiopia: A cross-sectional evaluation during July 2020","volume":"111","author":"Tadesse","year":"2021","journal-title":"Int. J. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e1517","DOI":"10.1016\/S2214-109X(21)00386-7","article-title":"Seroepidemiology and model-based prediction of SARS-CoV-2 in Ethiopia: Longitudinal cohort study among front-line hospital workers and communities","volume":"9","author":"Gudina","year":"2021","journal-title":"Lancet Glob. Health"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1163688","DOI":"10.3389\/fimmu.2023.1163688","article-title":"SARS-CoV-2 Infection-and mRNA Vaccine-induced Humoral Immunity among Schoolchildren in Hawassa, Ethiopia","volume":"14","author":"Merid","year":"2023","journal-title":"Front. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"100880","DOI":"10.1016\/j.eclinm.2021.100880","article-title":"Prevalence of SARS-CoV-2 in urban and rural Ethiopia: Randomized household serosurveys reveal level of spread during the first wave of the pandemic","volume":"35","author":"Abdella","year":"2021","journal-title":"eClinicalMedicine"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1200\/GO.20.00064","article-title":"Outcome of oncology patients infected with coronavirus","volume":"6","author":"Jazieh","year":"2020","journal-title":"JCO Glob. Oncol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1016\/j.annonc.2020.03.297","article-title":"COVID-19 infection in cancer patients: Early observations and unanswered questions","volume":"31","author":"Oh","year":"2020","journal-title":"Ann. Oncol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1182\/blood.2021012251","article-title":"COVID-19 in patients with hematologic malignancy","volume":"140","author":"Langerbeins","year":"2022","journal-title":"Blood"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1159\/000508199","article-title":"Treating leukemia in the time of COVID-19","volume":"144","author":"Paul","year":"2021","journal-title":"Acta Haematol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"6310","DOI":"10.1002\/cam4.4179","article-title":"The serological prevalence of SARS-CoV-2 infection in patients with chronic myeloid leukemia is similar to that in the general population","volume":"10","author":"Bonifacio","year":"2021","journal-title":"Cancer Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1182\/blood-2020-142454","article-title":"Prevalence of Sars-Cov-2 infection in patients with chronic myeloid leukemia","volume":"136","author":"Claudiani","year":"2020","journal-title":"Blood"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Kgatle, M., Das, R., Lawal, I., Boshomane, T., Mokoala, K., Gaspar, C., Mbokazi, L., Nkambule, N., Gow, V., and Ndlovu, H. (2023). The SARS-COV-2 Seroprevalence among Oncology Patients. J. Clin. Med., 12.","DOI":"10.3390\/jcm12020529"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"703","DOI":"10.3390\/covid3050052","article-title":"Epidemiology of SARS-CoV-2 Infection in Ethiopia: A Systematic Review and Meta-Analysis","volume":"3","author":"Gedefie","year":"2023","journal-title":"COVID"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Sisay, A., Tshiabuila, D., van Wyk, S., Tesfaye, A., Mboowa, G., Oyola, S.O., Tesema, S.K., Baxter, C., Martin, D., and Lessells, R. (2023). Molecular epidemiology and diversity of SARS-CoV-2 in Ethiopia, 2020\u20132022. Genes, 14.","DOI":"10.1101\/2023.01.30.23285174"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1038\/s41375-020-01103-2","article-title":"SARS-CoV-2 antibody responses in patients with acute leukaemia","volume":"35","author":"Muir","year":"2021","journal-title":"Leukemia"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1007\/s12250-020-00276-5","article-title":"Clinical characteristics of hospitalized patients with SARS-CoV-2 and hepatitis B virus co-infection","volume":"35","author":"Chen","year":"2020","journal-title":"Virol. Sin."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"100135","DOI":"10.1074\/jbc.REV120.015980","article-title":"Priming of SARS-CoV-2 S protein by several membrane-bound serine proteinases could explain enhanced viral infectivity and systemic COVID-19 infection","volume":"296","year":"2021","journal-title":"J. Biol. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sebasti\u00e1n-Mart\u00edn, A., S\u00e1nchez, B.G., Mora-Rodr\u00edguez, J.M., Bort, A., and D\u00edaz-Laviada, I. (2022). Role of dipeptidyl peptidase-4 (DPP4) on COVID-19 physiopathology. Biomedicines, 10.","DOI":"10.3390\/biomedicines10082026"}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/16\/8\/1606\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:32:13Z","timestamp":1760106733000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/16\/8\/1606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,22]]},"references-count":41,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,4]]}},"alternative-id":["cancers16081606"],"URL":"https:\/\/doi.org\/10.3390\/cancers16081606","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,22]]}}}