{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:31:50Z","timestamp":1791639110223,"version":"4.3.4"},"reference-count":30,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,4,28]],"date-time":"2024-04-28T00:00:00Z","timestamp":1714262400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>While ex vivo confocal laser scanning microscopy has previously demonstrated its utility in most common skin diseases, its use in the assessment of dermatological entities with lower incidence remains unexplored in most cases. We therefore aimed to evaluate the diagnostic efficacy of some rare skin tumors as well as a few inflammatory skin diseases, that have not yet been studied in ex vivo confocal laser scanning microscopy. A total of 50 tissue samples comprising 10 healthy controls, 10 basal cell carcinoma, 10 squamous cell carcinoma, and 20 rare skin conditions were imaged using the newest generation ex vivo confocal microscopy (Vivascope 2500 M-G4, Vivascope GmbH, Munich, Germany). Three blinded investigators were asked to identify characteristic features of rare skin disorders and distinguish them from more common skin diseases in the ex vivo confocal microscopy images. Our findings present the capability of ex vivo confocal microscopy to display distinctive morphologic patterns in common and rare skin diseases. As might be expected, we found a strong correlation between imaging experience and diagnostic accuracy. While the imaging inexperienced dermatohistopathologist reached 60% concordance, the imaging-trained dermatologist obtained 88% agreement with dermatohistopathology. The imaging-trained dermatohistopathologist achieved concordance up to 92% with gold-standard dermatohistopathology. This study highlights the potential of ex vivo confocal laser scanning microscopy as a promising adjunct to conventional dermatohistopathology for the early and precise identification of rare dermatological disorders.<\/jats:p>","DOI":"10.3390\/cancers16091713","type":"journal-article","created":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T06:13:22Z","timestamp":1714371202000},"page":"1713","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Ex Vivo Confocal Laser Scanning Microscopy in Rare Skin Diseases"],"prefix":"10.3390","volume":"16","author":[{"given":"Luis","family":"Messner","sequence":"first","affiliation":[{"name":"Department of Dermatology and Allergy, LMU University Hospital, LMU Munich, 80337 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4935-9877","authenticated-orcid":false,"given":"Maximilian","family":"Deu\u00dfing","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy, LMU University Hospital, LMU Munich, 80337 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Maurer","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy, LMU University Hospital, LMU Munich, 80337 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Buttgereit","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy, LMU University Hospital, LMU Munich, 80337 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"St\u00e4rr","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy, LMU University Hospital, LMU Munich, 80337 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars E.","family":"French","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy, LMU University Hospital, LMU Munich, 80337 Munich, Germany"},{"name":"Department of Dermatology & Cutaneous Surgery, Miller School of Medicine, University of Miami, Miami, FL 33136, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1002-8133","authenticated-orcid":false,"given":"Daniela","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergy, LMU University Hospital, LMU Munich, 80337 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1111\/jdv.18050","article-title":"Prevalence of most common skin diseases in Europe: A population-based study","volume":"36","author":"Richard","year":"2022","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2183","DOI":"10.1111\/jdv.16849","article-title":"The evolving field of Dermato-oncology and the role of dermatologists: Position Paper of the EADO, EADV and Task Forces, EDF, IDS, EBDV\u2013UEMS and EORTC Cutaneous Lymphoma Task Force","volume":"34","author":"Garbe","year":"2020","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Vladimirova, G., Ruini, C., Kapp, F., Kendziora, B., Erg\u00fcn, E.Z., Ba\u011fc\u0131, I.S., Krammer, S., Jastaneyah, J., Sattler, E.C., and Flaig, M.J. (2022). Ex vivo confocal laser scanning microscopy: A diagnostic technique for easy real-time evaluation of benign and malignant skin tumours. J. Biophotonics, 15.","DOI":"10.1002\/jbio.202100372"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"109","DOI":"10.5826\/dpc.0802a08","article-title":"Ex vivo confocal microscopy: An emerging technique in dermatology","volume":"8","author":"Cinotti","year":"2018","journal-title":"Dermatol. Pract. Concept."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1111\/bjd.12671","article-title":"Ex vivo fluorescence confocal microscopy for fast evaluation of tumour margins during Mohs surgery","volume":"170","author":"Vilata","year":"2014","journal-title":"Br. J. Dermatol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1111\/bjd.19017","article-title":"Ex vivo confocal microscopy: Revolution in fast pathology in dermatology","volume":"183","author":"Malvehy","year":"2020","journal-title":"Br J Dermatol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1111\/exd.14265","article-title":"New-generation diagnostics in inflammatory skin diseases: Immunofluorescence and histopathological assessment using ex vivo confocal laser scanning microscopy in cutaneous lupus erythematosus","volume":"30","author":"Aoki","year":"2021","journal-title":"Exp. Dermatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"586648","DOI":"10.3389\/fmed.2020.586648","article-title":"Ex vivo Confocal Laser Scanning Microscopy: A Potential New Diagnostic Imaging Tool in Onychomycosis Comparable with Gold Standard Techniques","volume":"7","author":"Krammer","year":"2020","journal-title":"Front. Med."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hartmann, D., Krammer, S., Vural, S., Bachmann, M.R., Ruini, C., S\u00e1rdy, M., Ruzicka, T., Berking, C., and von Braunm\u00fchl, T. (2018). Immunofluorescence and confocal microscopy for ex-vivo diagnosis of melanocytic and non-melanocytic skin tumors: A pilot study. J. Biophotonics, 11.","DOI":"10.1002\/jbio.201700211"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1111\/srt.12690","article-title":"Ex vivo confocal microscopy for dermatofibrosarcoma protuberans","volume":"25","author":"Lamberti","year":"2019","journal-title":"Skin Res. Technol."},{"key":"ref_11","first-page":"41","article-title":"Atypical fibroxanthoma: In-vivo and ex-vivo confocal features","volume":"156","author":"Pampena","year":"2021","journal-title":"Ital. J. Dermatol. Venerol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1111\/j.1365-2133.2012.10830.x","article-title":"A systematic review of worldwide incidence of nonmelanoma skin cancer","volume":"166","author":"Lomas","year":"2012","journal-title":"Br. J. Dermatol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.hoc.2018.08.001","article-title":"Cutaneous Squamous Cell Carcinoma","volume":"33","author":"Waldman","year":"2019","journal-title":"Hematol. Oncol. Clin. N. Am."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"S24","DOI":"10.1097\/DSS.0000000000000300","article-title":"Incidence and Survival of Primary Dermatofibrosarcoma Protuberans in the United States","volume":"42","author":"Kreicher","year":"2016","journal-title":"Dermatol. Surg."},{"key":"ref_15","first-page":"5717","article-title":"Atypical Fibroxanthoma\u2014Histological Diagnosis, Immunohistochemical Markers and Concepts of Therapy","volume":"35","author":"Koch","year":"2015","journal-title":"Anticancer Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1016\/j.ejca.2012.09.011","article-title":"Descriptive epidemiology of sarcomas in Europe: Report from the RARECARE project","volume":"49","author":"Stiller","year":"2013","journal-title":"Eur. J. Cancer"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1046\/j.1365-2133.2000.03925.x","article-title":"Cutaneous leiomyomas (piloleiomyomas) in adult patients with human immunodeficiency virus infection","volume":"143","author":"Kanitakis","year":"2000","journal-title":"Br. J. Dermatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1001\/archderm.143.7.854","article-title":"Incidence of cutaneous T-cell lymphoma in the United States, 1973-2002","volume":"143","author":"Criscione","year":"2007","journal-title":"Arch Dermatol"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/S0046-8177(03)00075-3","article-title":"Cutaneous lymphoid hyperplasia: A lymphoproliferative continuum with lymphomatous potential","volume":"34","author":"Nihal","year":"2003","journal-title":"Hum. Pathol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1559","DOI":"10.1016\/j.jaad.2020.04.183","article-title":"Prurigo nodularis: Epidemiology and clinical features","volume":"83","author":"Huang","year":"2020","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1111\/ijd.12600","article-title":"Cylindroma: An update","volume":"54","author":"Cuzzi","year":"2015","journal-title":"Int. J. Dermatol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4103\/2229-5178.93486","article-title":"Clinicoepidemiological study of pigmented purpuric dermatoses","volume":"3","author":"Sharma","year":"2012","journal-title":"Indian Dermatol. Online J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1177","DOI":"10.1016\/j.jaad.2023.08.050","article-title":"Atypical fibroxanthoma and pleomorphic dermal sarcoma: Local recurrence and metastasis in a nationwide population-based cohort of 1118 patients","volume":"89","author":"Abebe","year":"2023","journal-title":".J Am. Acad. Dermatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1504","DOI":"10.1097\/PAS.0b013e3181ef70b6","article-title":"Adult-type fibrosarcoma: A reevaluation of 163 putative cases diagnosed at a single institution over a 48-year period","volume":"34","author":"Bahrami","year":"2010","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_25","unstructured":"Robert Koch Institute (2021). Infektionsepidemiologischse Jahrbuch Meldepflichtiger Krankheiten f\u00fcr 2020, Robert Koch Institute."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.jaad.2013.04.047","article-title":"Primary cutaneous marginal zone B-cell lymphoma: Response to treatment and disease-free survival in a series of 137 patients","volume":"69","author":"Servitje","year":"2013","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.jaad.2021.01.044","article-title":"Six steps to reach optimal scanning in ex vivo confocal microscopy","volume":"86","author":"Toll","year":"2022","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e157","DOI":"10.1016\/j.jaad.2019.06.041","article-title":"A fast and effective option for tissue flattening: Optimizing time and efficacy in ex vivo confocal microscopy","volume":"82","author":"Puig","year":"2020","journal-title":"J Am Acad Dermatol"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"adv4859","DOI":"10.2340\/actadv.v103.4859","article-title":"Diagnosis of Basal Cell Carcinoma with Ex-vivo Confocal Laser Scanning Microscopy in a Real-life Setting","volume":"103","author":"Forchhammer","year":"2023","journal-title":"Acta Derm. Venereol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Ogrzewalla, H., M\u00f6hrle, M., Metzler, G., Eigentler, T., M\u00fcnch, A.K., and Forchhammer, S. (2022). A Feasibility Study for Immediate Histological Assessment of Various Skin Biopsies Using Ex Vivo Confocal Laser Scanning Microscopy. 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