{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:08:03Z","timestamp":1760144883220,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T00:00:00Z","timestamp":1717027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Leuk\u00e4mie Lymphom Liga e. V., Duesseldorf, Germany","award":["2019-21","DFG 417677437\/GRK2578"],"award-info":[{"award-number":["2019-21","DFG 417677437\/GRK2578"]}]},{"DOI":"10.13039\/100004807","name":"Research Committee of the Medical Faculty","doi-asserted-by":"publisher","award":["2019-21","DFG 417677437\/GRK2578"],"award-info":[{"award-number":["2019-21","DFG 417677437\/GRK2578"]}],"id":[{"id":"10.13039\/100004807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Myeloid and lymphoid neoplasms share the characteristics of potential bone marrow infiltration as a primary or secondary effect, which readily leads to hematopoietic insufficiency. The mechanisms by which clonal malignant cells inhibit normal hematopoietic stem and progenitor cells (HSPCs) in the bone marrow (BM) have not been unraveled so far. Given the pivotal role of mesenchymal stromal cells (MSCs) in the regulation of hematopoiesis in the BM niche it is assumed that MSCs also play a relevant role in the pathogenesis of hematological neoplasms. We aimed to identify overlapping mechanisms in MSCs derived from myeloid and lymphoid neoplasms contributing to disease progression and suppression of HSPCs to develop interventions that target these mechanisms. MSCs derived from healthy donors (n = 44) and patients diagnosed with myeloproliferative neoplasia (n = 11), myelodysplastic syndromes (n = 16), or acute myeloid leukemia (n = 25) and B-Non-Hodgkin lymphoma (n = 9) with BM infiltration and acute lymphoblastic leukemia (n = 9) were analyzed for their functionality and by RNA sequencing. A reduced growth and differentiation capacity of MSCs was found in all entities. RNA sequencing distinguished both groups but clearly showed overlapping differentially expressed genes, including major players in the BMP\/TGF and WNT-signaling pathway which are crucial for growth, osteogenesis, and hematopoiesis. Functional alterations in healthy MSCs were inducible by exposure to supernatants from malignant cells, implicating the involvement of these factors in disease progression. Overall, we were able to identify overlapping factors that pose potential future therapeutic targets.<\/jats:p>","DOI":"10.3390\/cancers16112071","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T06:08:14Z","timestamp":1717049294000},"page":"2071","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Overlapping Stromal Alterations in Myeloid and Lymphoid Neoplasms"],"prefix":"10.3390","volume":"16","author":[{"given":"Lucienne","family":"Bogun","sequence":"first","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annemarie","family":"Koch","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Scherer","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Germing","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Fenk","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uwe","family":"Maus","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery and Traumatology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5919-3375","authenticated-orcid":false,"given":"Felix","family":"Bormann","sequence":"additional","affiliation":[{"name":"Bioinformatics.Expert UG, 12305 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3644-2022","authenticated-orcid":false,"given":"Karl","family":"K\u00f6hrer","sequence":"additional","affiliation":[{"name":"Biological and Medical Research Center (BMFZ), Medical Faculty, Heinrich-Heine-University, Universit\u00e4tsstra\u00dfe 1, 40225 D\u00fcsseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8355-5524","authenticated-orcid":false,"given":"Patrick","family":"Petzsch","sequence":"additional","affiliation":[{"name":"Biological and Medical Research Center (BMFZ), Medical Faculty, Heinrich-Heine-University, Universit\u00e4tsstra\u00dfe 1, 40225 D\u00fcsseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6760-2458","authenticated-orcid":false,"given":"Thorsten","family":"Wachtmeister","sequence":"additional","affiliation":[{"name":"Biological and Medical Research Center (BMFZ), Medical Faculty, Heinrich-Heine-University, Universit\u00e4tsstra\u00dfe 1, 40225 D\u00fcsseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guido","family":"Kobbe","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sascha","family":"Dietrich","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3652-6595","authenticated-orcid":false,"given":"Rainer","family":"Haas","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Schroeder","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"Geyh","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9250-2064","authenticated-orcid":false,"given":"Paul","family":"J\u00e4ger","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Clinical Immunology, Medical Faculty, University of Duesseldorf, 40225 Duesseldorf, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1038\/s41375-022-01620-2","article-title":"The 5th edition of the World Health Organization Classification of Haematolymphoid Tumours: Lymphoid Neoplasms","volume":"36","author":"Alaggio","year":"2022","journal-title":"Leukemia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1182\/blood.2022015850","article-title":"International Consensus Classification of Myeloid Neoplasms and Acute Leukemias: 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