{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:12:22Z","timestamp":1760145142843,"version":"build-2065373602"},"reference-count":32,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:00:00Z","timestamp":1719532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fondo de Investigacion Sanitaria (FIS), Instituto de Salud Carlos III","award":["PI17\/313","PI23\/199"],"award-info":[{"award-number":["PI17\/313","PI23\/199"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background: LMO2 is a relevant gene involved in B-cell ontogeny and a survival predictor of aggressive large B-cell lymphomas (aLBCL). Most studies assessing LMO2 mRNA expression have relied on microarray platforms or qRT-PCR methods, overlooking tissue morphology. In this study, we evaluate LMO2 RNA expression by chromogenic in situ hybridization (CISH) in normal tissue and in a series of 82 aLBCL. Methods: LMO2 CISH was performed in formalin-fixed paraffin-embedded tissues, scored by three different methods, and correlated with a transcriptome panel. Results: We obtained statistically significant results correlating the methods of evaluation with LMO2 protein expression and gene expression results. Normal tonsil tissue showed high levels of LMO2, particularly within the light zone of the germinal center. Conversely, in aLBCL, a notable reduction in LMO2 expression was noted, remarkably in cases carrying MYC rearrangements. Furthermore, significant results were obtained through overall survival and Cox regression survival analysis, incorporating International Prognostic Index data alongside LMO2 expression levels. Conclusions: We show a reliable method to identify LMO2 mRNA expression by CISH, effectively capturing many of the reported biologic features of LMO2.<\/jats:p>","DOI":"10.3390\/cancers16132378","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T08:31:36Z","timestamp":1719563496000},"page":"2378","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Chromogenic LMO2 mRNA ISH Expression Correlates with LMO2 Protein and Gene Expression and Captures Their Survival Impact in Diffuse Large B-Cell Lymphoma, NOS"],"prefix":"10.3390","volume":"16","author":[{"given":"Natalia","family":"Papaleo","sequence":"first","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"},{"name":"Department of Pathology, Consorci Hospitalari Parc Tauli, Institut d\u2019Investigaci\u00f3 i Innovaci\u00f3 Parc Taul\u00ed (I3PT), 08208 Sabadell, Spain"},{"name":"Department of Morphological Sciences, Universitat Autonoma de Barcelona, 08193 Barcelona, Spain"},{"name":"Department of Health and Experimental Sciences, Universitat Pompeu Fabra, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2037-408X","authenticated-orcid":false,"given":"Andrea","family":"Molina-Alvarez","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricard","family":"Onieva","sequence":"additional","affiliation":[{"name":"Department of Pathology, Consorci Hospitalari Parc Tauli, Institut d\u2019Investigaci\u00f3 i Innovaci\u00f3 Parc Taul\u00ed (I3PT), 08208 Sabadell, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0512-4235","authenticated-orcid":false,"given":"Diana","family":"Fuertes","sequence":"additional","affiliation":[{"name":"Research Unit Support, Institut d\u2019Investigaci\u00f3 i Innovaci\u00f3 Parc Taul\u00ed (I3PT), 08208 Sabadell, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Blanca","family":"Sanchez-Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Hematology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xenia","family":"Riera","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Lopez-Segura","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Lome-Maldonado","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6160-6069","authenticated-orcid":false,"given":"Xavier","family":"Ara-Mancebo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1195-1496","authenticated-orcid":false,"given":"Jose","family":"Yelamos","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9988-5977","authenticated-orcid":false,"given":"Marta","family":"Salido","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivonne","family":"Vazquez","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7934-9130","authenticated-orcid":false,"given":"Xavier","family":"Calvo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5236-5085","authenticated-orcid":false,"given":"Luis","family":"Colomo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Hospital del Mar, Hospital del Mar Research Institute-IMIM, 08003 Barcelona, Spain"},{"name":"Department of Health and Experimental Sciences, Universitat Pompeu Fabra, 08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,28]]},"reference":[{"key":"ref_1","first-page":"1887","article-title":"TTG-2, a New Gene Encoding a Cysteine-Rich Protein 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