{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:13:04Z","timestamp":1760145184197,"version":"build-2065373602"},"reference-count":55,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:00:00Z","timestamp":1719532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"DAAD","award":["21520154"],"award-info":[{"award-number":["21520154"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Hypoxia can modulate the immune system by affecting the function and activity of immune cells, potentially leading to altered immune responses. This study investigated the generation of leukemia-derived dendritic cells (DCleu) from leukemic blasts and their impact on immune cell activation under hypoxic (5\u201310% O2) compared to normoxic (21% O2) conditions using various immunomodulatory Kits. The results revealed that DC\/DCleu-generation was similar under hypoxic and normoxic conditions, with no significant differences observed in frequencies of generated DC\/DCleu. Furthermore, the study showed that the activation of immune cells and their anti-leukemic activity improved when T cell-enriched immunoreactive cells were co-cultured with DC\/DCleu which were generated with Kit I and M compared to the control after mixed lymphocyte cultures. The anti-leukemic activity was improved under hypoxic compared to normoxic conditions after MLCWB-DC Kit M. These findings suggest that DC\/DCleu-cultures of leukemic whole blood with Kits under hypoxic conditions yield comparable frequencies of DC\/DCleu and can even increase the anti-leukemic activity compared to normoxic conditions. Overall, this research highlights the potential of utilizing DC\/DCleu (potentially induced in vivo with Kits) as a promising approach to enhance immune response in patients with acute myeloid leukemia.<\/jats:p>","DOI":"10.3390\/cancers16132383","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T08:31:36Z","timestamp":1719563496000},"page":"2383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Anti-Leukemic Effects Induced by Dendritic Cells of Leukemic Origin from Leukemic Blood Samples Are Comparable under Hypoxic vs. Normoxic Conditions"],"prefix":"10.3390","volume":"16","author":[{"given":"Fatemeh","family":"Doraneh-Gard","sequence":"first","affiliation":[{"name":"Medical Department III, Working-group Immune-Modulation, Klinikum Gro\u00dfhadern, Ludwig-Maximilians-University, 81377 Munich, Germany"},{"name":"Bavarian Cancer Research Center (BZKF), 86156 Augsburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Christoph","family":"Amberger","sequence":"additional","affiliation":[{"name":"First Department of Medicine, Paracelsus Medical University, 5020 Salzburg, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carina","family":"Amend","sequence":"additional","affiliation":[{"name":"Medical Department III, Working-group Immune-Modulation, Klinikum Gro\u00dfhadern, Ludwig-Maximilians-University, 81377 Munich, Germany"},{"name":"Bavarian Cancer Research Center (BZKF), 86156 Augsburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Weinmann","sequence":"additional","affiliation":[{"name":"Medical Department III, Working-group Immune-Modulation, Klinikum Gro\u00dfhadern, Ludwig-Maximilians-University, 81377 Munich, Germany"},{"name":"Bavarian Cancer Research Center (BZKF), 86156 Augsburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Schwepcke","sequence":"additional","affiliation":[{"name":"Medical Department III, Working-group Immune-Modulation, Klinikum Gro\u00dfhadern, Ludwig-Maximilians-University, 81377 Munich, Germany"},{"name":"Bavarian Cancer Research Center (BZKF), 86156 Augsburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8560-7447","authenticated-orcid":false,"given":"Lara","family":"Klauer","sequence":"additional","affiliation":[{"name":"Medical Department III, Working-group Immune-Modulation, Klinikum Gro\u00dfhadern, Ludwig-Maximilians-University, 81377 Munich, Germany"},{"name":"Bavarian Cancer Research Center (BZKF), 86156 Augsburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olga","family":"Schutti","sequence":"additional","affiliation":[{"name":"Medical Department III, Working-group Immune-Modulation, Klinikum Gro\u00dfhadern, Ludwig-Maximilians-University, 81377 Munich, Germany"},{"name":"Bavarian Cancer Research Center (BZKF), 86156 Augsburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1178-618X","authenticated-orcid":false,"given":"Hedayatollah","family":"Hosseini","sequence":"additional","affiliation":[{"name":"Experimental Medicine and Therapy Research Department, Faculty of Medicine, University of Regensburg, 93040 Regensburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doris","family":"Kr\u00e4mer","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology and Palliative Care, Hospital Hagen, 58097 Hagen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5636-0043","authenticated-orcid":false,"given":"Andreas","family":"Rank","sequence":"additional","affiliation":[{"name":"Bavarian Cancer Research Center (BZKF), 86156 Augsburg, Germany"},{"name":"Department of Hematology and Oncology, University Hospital of Augsburg, 86156 Augsburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Schmid","sequence":"additional","affiliation":[{"name":"Bavarian Cancer Research Center (BZKF), 86156 Augsburg, Germany"},{"name":"Department of Hematology and Oncology, University Hospital of Augsburg, 86156 Augsburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helga Maria","family":"Schmetzer","sequence":"additional","affiliation":[{"name":"Medical Department III, Working-group Immune-Modulation, Klinikum Gro\u00dfhadern, Ludwig-Maximilians-University, 81377 Munich, Germany"},{"name":"Bavarian Cancer Research Center (BZKF), 86156 Augsburg, 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