{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:48:21Z","timestamp":1791236901949,"version":"4.1.0"},"reference-count":90,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T00:00:00Z","timestamp":1747699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"JSPS KAKENHI","award":["24K02588"],"award-info":[{"award-number":["24K02588"]}]},{"name":"S-SPRING (Japan Science and Technology Agency (JST))","award":["24K02588"],"award-info":[{"award-number":["24K02588"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background\/objective: Mucinous borderline tumors of the ovary (MBOTs) are characterized by their unique histological features and intermediate malignant potential; however, the factors underlying their molecular carcinogenesis and tumor biology remain largely unknown. Developing cell lines from these tumors presents an ongoing challenge. The purpose of this study is to establish MBOT cell lines and characterize their biological features. Methods: Epithelial cells were collected and purified from surgically removed MBOT samples and then stably maintained with an extended life span by overexpressing CyclinD1\/CDK4 in combination with human telomerase reverse transcriptase. The characterization of resulting cell lines was defined by morphology, growth kinetics, functional analysis, whole-exome sequencing, and tumorigenicity in mice. Results: Two independent cell lines, HMucBOT-1 and HMucBOT-2, were successfully established from the tissues of a patient with an MBOT, with the latter showing more aggressive growth capacity. In the patient-derived xenograft model, HMucBOT-1 cells retained the original morphological characteristics of the MBOT, whereas HMucBOT-2 cells displayed a transition to mucinous carcinoma accompanying undifferentiated carcinoma, suggestive of dedifferentiated carcinoma. Genetic analysis of the original tumor sample and HMucBOT-2 cells revealed shared oncogenic mutations. However, KRAS amplification and certain copy number alterations were uniquely observed in the HMucBOT-2 cells. Conclusions: The above results indicate that HMucBOT-1 can serve as a preclinical model for investigating the biological behavior of and potential targeted therapies for human MBOTs, with HMucBOT-2 serving as a valuable tool for studying the heterogeneity and genetic diversity of this tumor and explaining the potential causes of treatment failure or relapse.<\/jats:p>","DOI":"10.3390\/cancers17101716","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T11:50:31Z","timestamp":1747741831000},"page":"1716","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Establishment of Two Novel Ovarian Tumor Cell Lines with Characteristics of Mucinous Borderline Tumors or Dedifferentiated Carcinoma\u2014Implications for Tumor Heterogeneity and the Complex Carcinogenesis of Mucinous Tumors"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-8987-9686","authenticated-orcid":false,"given":"Hasibul Islam","family":"Sohel","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7147-8569","authenticated-orcid":false,"given":"Umme Farzana","family":"Zahan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1769-403X","authenticated-orcid":false,"given":"Tohru","family":"Kiyono","sequence":"additional","affiliation":[{"name":"Exploratory Oncology Research and Clinical Trial Center (EPOC), National Cancer Center, Kashiwa 277-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masako","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sultana","family":"Razia","sequence":"additional","affiliation":[{"name":"Department of Legal Medicine, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Kanno","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitomi","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9983-5810","authenticated-orcid":false,"given":"Shahataj Begum","family":"Sonia","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3250-3097","authenticated-orcid":false,"given":"Kentaro","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, East Medical Center, Nagoya City University, Nagoya 464-8547, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8986-6147","authenticated-orcid":false,"given":"Satoru","family":"Kyo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, Shimane University, Izumo 693-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"229","DOI":"10.3322\/caac.21834","article-title":"Global Cancer Statistics 2022: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3322\/caac.21871","article-title":"Cancer Statistics, 2025","volume":"75","author":"Siegel","year":"2025","journal-title":"CA Cancer J. Clin."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"507","DOI":"10.3332\/ecancer.2015.507","article-title":"Fertility Sparing Treatment in Borderline Ovarian Tumours","volume":"9","author":"Alvarez","year":"2015","journal-title":"Ecancermedicalscience"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s00520-018-4327-6","article-title":"The Role of Positive Psychological Changes in Anxiety and Depression of Patients with Ovarian Tumors and Their Partners: An Observational Study from the Population-Based PROFILES Registry","volume":"27","author":"Camara","year":"2019","journal-title":"Support. Care Cancer"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1007\/s00428-016-2040-8","article-title":"Ovarian Borderline Tumors in the 2014 WHO Classification: Evolving Concepts and Diagnostic Criteria","volume":"470","author":"Hauptmann","year":"2017","journal-title":"Virchows Arch."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/j.humpath.2004.03.003","article-title":"Borderline Ovarian Tumors: Key Points and Workshop Summary","volume":"35","author":"Silverberg","year":"2004","journal-title":"Hum. Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"143","DOI":"10.47162\/RJME.64.2.03","article-title":"Conservative Treatment of Borderline Ovarian Tumors: A Retrospective Study","volume":"64","author":"Constantinescu","year":"2023","journal-title":"Rom. J. Morphol. Embryol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.humpath.2004.03.004","article-title":"Borderline Ovarian Tumors: Diverse Contemporary Viewpoints on Terminology and Diagnostic Criteria with Illustrative Images","volume":"35","author":"Seidman","year":"2004","journal-title":"Hum. Pathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"44","DOI":"10.3802\/jgo.2013.24.1.44","article-title":"Histologic Distribution of Borderline Ovarian Tumors Worldwide: A Systematic Review","volume":"24","author":"Song","year":"2013","journal-title":"J. Gynecol. Oncol."},{"key":"ref_10","unstructured":"Young, R. (2014). WHO Classification of Tumours of Female Reproductive Organs, IARC. [2014th ed.]."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1631","DOI":"10.1111\/jog.14724","article-title":"Board members of the 2020 Committee on Gynecologic Oncology of the Japan Society of Obstetrics and Gynecology Annual Report of the Committee on Gynecologic Oncology, the Japan Society of Obstetrics and Gynecology: Annual Patient Report for 2017 and Annual Treatment Report for 2012","volume":"47","author":"Nagase","year":"2021","journal-title":"J. Obstet. Gynaecol. Res."},{"key":"ref_12","unstructured":"WHO Classification of Tumours Editorial Board (2020). Female Genital Tumours, WHO."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.mric.2022.06.003","article-title":"MR Imaging of Epithelial Ovarian Neoplasms Part I: Benign and Borderline","volume":"31","author":"Wahab","year":"2023","journal-title":"Magn. Reson. Imaging Clin. North. Am."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<145::AID-PATH420>3.0.CO;2-F","article-title":"Tumors of the Ovary, Maldeveloped Gonads, Fallopian Tube and Broad Ligament. Atlas of Tumor Pathology. Third Series, Fascicle 23 Robert E. Scully, Robert H. Young and Philip B. Clement. Armed Forces Institute of Pathology, Washington, DC, 1998. No. of Pages: 527. Price: $95.00. ISBN: 1881041433","volume":"189","author":"Herrington","year":"1999","journal-title":"J. Pathol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1643","DOI":"10.1097\/IGC.0000000000001362","article-title":"Clinicopathologic Features and Risk Factors for Recurrence of Mucinous Borderline Ovarian Tumors: A Retrospective Study with Follow-up of More Than 10 Years","volume":"28","author":"Sun","year":"2018","journal-title":"Int. J. Gynecol. Cancer"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.ygyno.2016.05.005","article-title":"Relapse and Disease Specific Survival in 1143 Danish Women Diagnosed with Borderline Ovarian Tumours (BOT)","volume":"142","author":"Karlsen","year":"2016","journal-title":"Gynecol. Oncol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1097\/IGC.0b013e31824b4076","article-title":"Prognosis in Patients with Serous and Mucinous Stage I Borderline Ovarian Tumors","volume":"22","author":"Song","year":"2012","journal-title":"Int. J. Gynecol. Cancer"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1111\/jog.16085","article-title":"Oncological Outcomes and Risk Factors for Recurrence of Mucinous Borderline Ovarian Tumors: A 15-Year Experience at a Tertiary Center","volume":"50","author":"Poonyakanok","year":"2024","journal-title":"J. Obstet. Gynaecol. Res."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Nakagawa, K., Nakayama, K., Nakamura, A., Hadano, N., Kurose, S., Razia, S., Aoki, S., and Kyo, S. (2022). A Novel Case of Recurrent Mucinous Borderline Ovarian Tumor: Early Relapse and Fatal Outcome. Reports, 5.","DOI":"10.3390\/reports5020015"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1007\/s13691-024-00722-1","article-title":"A Case Report of Mucinous Borderline Ovarian Tumor with Recurrence as Invasive Carcinoma with High Copy Number Alterations","volume":"13","author":"Wakazono","year":"2024","journal-title":"Int. Cancer Conf. J."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Mackenzie, R., Kommoss, S., Winterhoff, B.J., Kipp, B.R., Garcia, J.J., Voss, J., Halling, K., Karnezis, A., Senz, J., and Yang, W. (2015). Targeted Deep Sequencing of Mucinous Ovarian Tumors Reveals Multiple Overlapping RAS-Pathway Activating Mutations in Borderline and Cancerous Neoplasms. BMC Cancer, 15.","DOI":"10.1186\/s12885-015-1421-8"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e70601","DOI":"10.1002\/cam4.70601","article-title":"Transcriptome Concordance Between Borderline Tumors and Endometrioid Carcinoma: An Integrative Genomic Analysis","volume":"14","author":"Takahashi","year":"2025","journal-title":"Cancer Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1002\/path.4088","article-title":"Molecular Characterization of Mucinous Ovarian Tumours Supports a Stratified Treatment Approach with HER2 Targeting in 19% of Carcinomas","volume":"229","author":"Anglesio","year":"2013","journal-title":"J. Pathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.12688\/f1000research.126337.1","article-title":"Role of RAS Signaling in Ovarian Cancer","volume":"11","author":"Therachiyil","year":"2022","journal-title":"F1000Res"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"82097","DOI":"10.18632\/oncotarget.13449","article-title":"Multipoint Kras Oncogene Mutations Potentially Indicate Mucinous Carcinoma on the Entire Spectrum of Mucinous Ovarian Neoplasms","volume":"7","author":"Lee","year":"2016","journal-title":"Oncotarget"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.cancergen.2024.06.002","article-title":"Cancer Treatments: Past, Present, and Future","volume":"286\u2013287","author":"Sonkin","year":"2024","journal-title":"Cancer Genet."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Hossain, M.M., Nakayama, K., Shanta, K., Razia, S., Ishikawa, M., Ishibashi, T., Yamashita, H., Sato, S., Iida, K., and Kanno, K. (2021). Establishment of a Novel In Vitro Model of Endometriosis with Oncogenic KRAS and PIK3CA Mutations for Understanding the Underlying Biology and Molecular Pathogenesis. Cancers, 13.","DOI":"10.3390\/cancers13133174"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Sohel, H.I., Kiyono, T., Zahan, U.F., Razia, S., Ishikawa, M., Yamashita, H., Kanno, K., Sonia, S.B., Nakayama, K., and Kyo, S. (2025). Establishment of a Novel In Vitro and In Vivo Model to Understand Molecular Carcinogenesis of Endometriosis-Related Ovarian Neoplasms. Int. J. Mol. Sci., 26.","DOI":"10.20944\/preprints202502.0858.v1"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1038\/bjc.2012.26","article-title":"Creation of Immortalised Epithelial Cells from Ovarian Endometrioma","volume":"106","author":"Bono","year":"2012","journal-title":"Br. J. Cancer"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1126\/science.7652577","article-title":"A p16INK4a-Insensitive CDK4 Mutant Targeted by Cytolytic T Lymphocytes in a Human Melanoma","volume":"269","author":"Hauer","year":"1995","journal-title":"Science"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1016\/j.stemcr.2017.05.013","article-title":"Fetal Therapy Model of Myelomeningocele with Three-Dimensional Skin Using Amniotic Fluid Cell-Derived Induced Pluripotent Stem Cells","volume":"8","author":"Kajiwara","year":"2017","journal-title":"Stem Cell Rep."},{"key":"ref_32","first-page":"1285","article-title":"Expression and Cisplatin Sensitivity of Copper-Transporting P-Type Adenosine Triphosphatase (ATP7B) in Human Solid Carcinoma Cell Lines","volume":"8","author":"Nakayama","year":"2001","journal-title":"Oncol. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/gt.2011.44","article-title":"CDK4 and Cyclin D1 Allow Human Myogenic Cells to Recapture Growth Property without Compromising Differentiation Potential","volume":"18","author":"Shiomi","year":"2011","journal-title":"Gene Ther."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1038\/nprot.2012.097","article-title":"Primary Culture and Immortalization of Human Fallopian Tube Secretory Epithelial Cells","volume":"7","author":"Karst","year":"2012","journal-title":"Nat. Protoc."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1038\/sj.bjc.6602662","article-title":"Establishment of an Immortalised Human Ovarian Surface Epithelial Cell Line without Chromosomal Instability","volume":"93","author":"Maeda","year":"2005","journal-title":"Br. J. Cancer"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Narayanan, B., Buddenkote, T., Smith, H., Shah, M., Freeman, S., Hulse, D., Funingana, G., Alcaraz, M.-L., Ortuzar, M.-C., and Brenton, J. (2024). Growth Kinetics of High-Grade Serous Ovarian Cancer Using Longitudinal Clinical Data\u2014Implications for Early Detection. medRxiv.","DOI":"10.1101\/2024.11.18.24317171"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1177\/1933719114557893","article-title":"Establishment of a New Ovarian Cancer Cell Line CA5171","volume":"22","author":"Chiang","year":"2015","journal-title":"Reprod. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1016\/j.lungcan.2011.01.029","article-title":"Frequency and Clinicopathologic Correlates of KRAS Amplification in Non-Small Cell Lung Carcinoma","volume":"74","author":"Wagner","year":"2011","journal-title":"Lung Cancer"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Rehkaemper, J., Korenkov, M., Quaas, A., Rueschoff, J., Pamuk, A., Zander, T., Hillmer, A.M., Buettner, R., Hoelscher, A.H., and Bruns, C.J. (2020). Amplification of KRAS and Its Heterogeneity in Non-Asian Gastric Adenocarcinomas. BMC Cancer, 20.","DOI":"10.1186\/s12885-020-06996-x"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1038\/bjc.2012.477","article-title":"KRAS Gene Amplification and Overexpression but Not Mutation Associates with Aggressive and Metastatic Endometrial Cancer","volume":"107","author":"Birkeland","year":"2012","journal-title":"Br. J. Cancer"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1002\/dc.25184","article-title":"Undifferentiated Component of Dedifferentiated Endometrial Carcinoma Presenting as a \u201cSmall Round Cell\u201d Malignancy in a Mediastinal Lymph Node, Mimicking Small Cell Carcinoma","volume":"51","author":"Balikani","year":"2023","journal-title":"Diagn. Cytopathol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"100374","DOI":"10.1016\/j.modpat.2023.100374","article-title":"Dedifferentiated and Undifferentiated Ovarian Carcinoma: An Aggressive and Molecularly Distinct Ovarian Tumor Characterized by Frequent SWI\/SNF Complex Inactivation","volume":"37","author":"Kommoss","year":"2024","journal-title":"Mod. Pathol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"101188","DOI":"10.1016\/j.gore.2023.101188","article-title":"Dedifferentiated Endometrial Carcinoma Arising from Serous Carcinoma: Diagnostic Challenges and Recommendations","volume":"47","author":"Xu","year":"2023","journal-title":"Gynecol. Oncol. Rep."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Kim, S., Bae, H., and Kim, H.-S. (2024). Dedifferentiated Leiomyosarcoma of the Uterine Corpus with Heterologous Component: Clinicopathological Analysis of Five Consecutive Cases from a Single Institution and Comprehensive Literature Review. Diagnostics, 14.","DOI":"10.3390\/diagnostics14020160"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.ehpc.2019.01.002","article-title":"Dedifferentiated Endometrial Adenocarcinoma with Neuroendocrine Differentiation and Ballooning-Cell Features: Report of a Rare Entity with an Unusual Histology","volume":"15","author":"Makhdoum","year":"2019","journal-title":"Human. Pathol. Case Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.ygyno.2020.11.015","article-title":"Clinico-Pathological Features Associated with Mismatch Repair Deficiency in Endometrial Undifferentiated\/Dedifferentiated Carcinoma: A Systematic Review and Meta-Analysis","volume":"160","author":"Travaglino","year":"2021","journal-title":"Gynecol. Oncol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1038\/modpathol.2010.41","article-title":"Endometrial and Ovarian Carcinomas with Undifferentiated Components: Clinically Aggressive and Frequently Underrecognized Neoplasms","volume":"23","author":"Tafe","year":"2010","journal-title":"Mod. Pathol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Ono, R., Nakayama, K., Nakamura, K., Yamashita, H., Ishibashi, T., Ishikawa, M., Minamoto, T., Razia, S., Ishikawa, N., and Otsuki, Y. (2019). Dedifferentiated Endometrial Carcinoma Could Be A Target for Immune Checkpoint Inhibitors (Anti PD-1\/PD-L1 Antibodies). Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20153744"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1007\/s43032-022-01143-2","article-title":"Seromucinous and Mucinous Borderline Ovarian Tumors: We Need to Know More","volume":"30","author":"Laban","year":"2023","journal-title":"Reprod. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1007\/s13193-023-01849-y","article-title":"Clinicopathological and Oncological Outcomes of Borderline Mucinous Tumours of Ovary: A Large Case Series","volume":"15","author":"Shylasree","year":"2024","journal-title":"Indian. J. Surg. Oncol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1038\/nrc2820","article-title":"Cell Line-Based Platforms to Evaluate the Therapeutic Efficacy of Candidate Anticancer Agents","volume":"10","author":"Sharma","year":"2010","journal-title":"Nat. Rev. Cancer"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"He, J., Zhang, C., Ozkan, A., Feng, T., Duan, P., Wang, S., Yang, X., Xie, J., and Liu, X. (2023). Patient-Derived Tumor Models and Their Distinctive Applications in Personalized Drug Therapy. Mechanobiol. Med., 1.","DOI":"10.1016\/j.mbm.2023.100014"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"100809","DOI":"10.1016\/j.gore.2021.100809","article-title":"Borderline Ovarian Mucinous Tumor with Anaplastic Carcinomatous Mural Nodule: A Case Report","volume":"37","author":"Menon","year":"2021","journal-title":"Gynecol. Oncol. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s13000-021-01079-2","article-title":"Primary Mucinous Ovarian Tumors vs. Ovarian Metastases from Gastrointestinal Tract, Pancreas and Biliary Tree: A Review of Current Problematics","volume":"16","author":"Dundr","year":"2021","journal-title":"Diagn. Pathol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"293","DOI":"10.3892\/ijmm.2017.3009","article-title":"Lewis(y) Antigen Promotes the Progression of Epithelial Ovarian Cancer by Stimulating MUC1 Expression","volume":"40","author":"Hou","year":"2017","journal-title":"Int. J. Mol. Med."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"161","DOI":"10.3892\/mco.2022.2594","article-title":"Expression of MUC1 in Different Tumours and Its Clinical Significance (Review)","volume":"17","author":"Lan","year":"2022","journal-title":"Mol. Clin. Oncol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"34","DOI":"10.7150\/jca.100922","article-title":"Technical and Biological Biases in Bulk Transcriptomic Data Mining for Cancer Research","volume":"16","author":"Liu","year":"2025","journal-title":"J. Cancer"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1097\/PGP.0b013e31823f8482","article-title":"Endocervical-Type Mucinous Borderline Tumors Are Related to Endometrioid Tumors Based on Mutation and Loss of Expression of ARID1A","volume":"31","author":"Wu","year":"2012","journal-title":"Int. J. Gynecol. Pathol."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Fontana, B., Gallerani, G., Salamon, I., Pace, I., Roncarati, R., and Ferracin, M. (2023). ARID1A in Cancer: Friend or Foe?. Front. Oncol., 13.","DOI":"10.3389\/fonc.2023.1136248"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/s12943-024-02216-3","article-title":"Targeting KRAS: From Metabolic Regulation to Cancer Treatment","volume":"24","author":"Shi","year":"2025","journal-title":"Mol. Cancer"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1038\/modpathol.2013.71","article-title":"KRAS Mutant Allele-Specific Imbalance Is Associated with Worse Prognosis in Pancreatic Cancer and Progression to Undifferentiated Carcinoma of the Pancreas","volume":"26","author":"Krasinskas","year":"2013","journal-title":"Mod. Pathol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/nature12634","article-title":"Mutational Landscape and Significance across 12 Major Cancer Types","volume":"502","author":"Kandoth","year":"2013","journal-title":"Nature"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1038\/ng.3564","article-title":"Distinct Patterns of Somatic Genome Alterations in Lung Adenocarcinomas and Squamous Cell Carcinomas","volume":"48","author":"Campbell","year":"2016","journal-title":"Nat. Genet."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/nature11125","article-title":"The Mutational Landscape of Lethal Castration-Resistant Prostate Cancer","volume":"487","author":"Grasso","year":"2012","journal-title":"Nature"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1038\/nature11327","article-title":"Subgroup-Specific Structural Variation across 1000 Medulloblastoma Genomes","volume":"488","author":"Northcott","year":"2012","journal-title":"Nature"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1126\/science.1198056","article-title":"The Genetic Landscape of the Childhood Cancer Medulloblastoma","volume":"331","author":"Parsons","year":"2011","journal-title":"Science"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1038\/nature11329","article-title":"Medulloblastoma Exome Sequencing Uncovers Subtype-Specific Somatic Mutations","volume":"488","author":"Pugh","year":"2012","journal-title":"Nature"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/nature11284","article-title":"Dissecting the Genomic Complexity Underlying Medulloblastoma","volume":"488","author":"Jones","year":"2012","journal-title":"Nature"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/nature11213","article-title":"Novel Mutations Target Distinct Subgroups of Medulloblastoma","volume":"488","author":"Robinson","year":"2012","journal-title":"Nature"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1038\/nature10351","article-title":"Frequent Mutation of Histone-Modifying Genes in Non-Hodgkin Lymphoma","volume":"476","author":"Morin","year":"2011","journal-title":"Nature"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1038\/nature08672","article-title":"Systematic Sequencing of Renal Carcinoma Reveals Inactivation of Histone Modifying Genes","volume":"463","author":"Dalgliesh","year":"2010","journal-title":"Nature"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1038\/ng.907","article-title":"Frequent Mutations of Chromatin Remodeling Genes in Transitional Cell Carcinoma of the Bladder","volume":"43","author":"Gui","year":"2011","journal-title":"Nat. Genet."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"2496","DOI":"10.1038\/s41467-018-04950-x","article-title":"KMT2D\/MLL2 Inactivation Is Associated with Recurrence in Adult-Type Granulosa Cell Tumors of the Ovary","volume":"9","author":"Hillman","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1038\/nature16965","article-title":"Genomic Analyses Identify Molecular Subtypes of Pancreatic Cancer","volume":"531","author":"Bailey","year":"2016","journal-title":"Nature"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"The Cancer Genome Atlas Research Network, Kim, J., Bowlby, R., Mungall, A.J., Robertson, A.G., Odze, R.D., Cherniack, A.D., Shih, J., Pedamallu, C.S., and Cibulskis, C. (2017). Integrated Genomic Characterization of Oesophageal Carcinoma. Nature, 541, 169\u2013175.","DOI":"10.1038\/nature20805"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"The Cancer Genome Atlas Research Network, Bass, A.J., Thorsson, V., Shmulevich, I., Reynolds, S.M., Miller, M., Bernard, B., Hinoue, T., Laird, P.W., and Curtis, C. (2014). Comprehensive Molecular Characterization of Gastric Adenocarcinoma. Nature, 513, 202\u2013209.","DOI":"10.1038\/nature13480"},{"key":"ref_77","doi-asserted-by":"crossref","unstructured":"Lee, Y., Moon, S., Seok, J.Y., Lee, J.-H., Nam, S., and Chung, Y.S. (2023). Characterization of the Genomic Alterations in Poorly Differentiated Thyroid Cancer. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-46466-5"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1369136","DOI":"10.3389\/fmed.2024.1369136","article-title":"Targeting KRAS Mutations in Pancreatic Cancer: Opportunities for Future Strategies","volume":"11","author":"Linehan","year":"2024","journal-title":"Front Med."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1016\/j.molmed.2024.04.017","article-title":"Copy Number Alterations: A Catastrophic Orchestration of the Breast Cancer Genome","volume":"30","author":"Shahrouzi","year":"2024","journal-title":"Trends Mol. Med."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1186\/s13073-022-01080-4","article-title":"Accumulation of Copy Number Alterations and Clinical Progression across Advanced Prostate Cancer","volume":"14","author":"Grist","year":"2022","journal-title":"Genome Med."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"6350","DOI":"10.1158\/1078-0432.CCR-20-1497","article-title":"Copy Number Alteration Burden Differentially Impacts Immune Profiles and Molecular Features of Hepatocellular Carcinoma","volume":"26","author":"Bassaganyas","year":"2020","journal-title":"Clin. Cancer Res."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"i53","DOI":"10.1093\/annonc\/mdw087","article-title":"Mucinous Epithelial Ovarian Carcinoma","volume":"27","author":"Perren","year":"2016","journal-title":"Ann. Oncol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.bpobgyn.2016.08.007","article-title":"Pathology of Borderline and Invasive Cancers","volume":"41","author":"Prat","year":"2017","journal-title":"Best. Pract. Res. Clin. Obstet. Gynaecol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1002\/(SICI)1097-0142(19970415)79:8<1581::AID-CNCR21>3.0.CO;2-T","article-title":"K-Ras Mutations in Mucinous Ovarian Tumors: A Clinicopathologic and Molecular Study of 95 Cases","volume":"79","author":"Cuatrecasas","year":"1997","journal-title":"Cancer"},{"key":"ref_85","first-page":"777","article-title":"K-Ras Activation Occurs Frequently in Mucinous Adenocarcinomas and Rarely in Other Common Epithelial Tumors of the Human Ovary","volume":"139","author":"Enomoto","year":"1991","journal-title":"Am. J. Pathol."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Xue, W., Wu, K., Guo, X., Chen, C., Huang, T., Li, L., Liu, B., Chang, H., and Zhao, J. (2024). The Pan-Cancer Landscape of Glutamate and Glutamine Metabolism: A Comprehensive Bioinformatic Analysis across 32 Solid Cancer Types. Biochim. Biophys. Acta Mol. Basis Dis., 1870.","DOI":"10.1016\/j.bbadis.2023.166982"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.cancergen.2024.09.001","article-title":"Updates on Liquid Biopsies in Neuroblastoma for Treatment Response, Relapse and Recurrence Assessment","volume":"288\u2013289","author":"Jahangiri","year":"2024","journal-title":"Cancer Genet."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.cancergen.2023.12.002","article-title":"Development of a Molecular Barcode Detection System for Pancreaticobiliary Malignancies and Comparison with Next-Generation Sequencing","volume":"280\u2013281","author":"Ohyama","year":"2024","journal-title":"Cancer Genet."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.cancergen.2023.12.003","article-title":"Methylation Signatures as Biomarkers for Non-Invasive Early Detection of Breast Cancer: A Systematic Review of the Literature","volume":"282\u2013283","author":"Gonzalez","year":"2024","journal-title":"Cancer Genet."},{"key":"ref_90","doi-asserted-by":"crossref","unstructured":"He, S., Xiao, X., Lei, R., Chen, J., Huang, H., Yilihamu, A., Guo, M., Tan, C., Li, X., and Zhuang, Z. (2023). Establishment of Breast Phyllodes Tumor Cell Lines Preserving the Features of Phyllodes Tumors. BIO Integr., 4.","DOI":"10.15212\/bioi-2022-0025"}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/17\/10\/1716\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:36:01Z","timestamp":1760031361000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/17\/10\/1716"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,20]]},"references-count":90,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["cancers17101716"],"URL":"https:\/\/doi.org\/10.3390\/cancers17101716","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,20]]}}}