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The methods used in this system are acoustic microscopy and infrared (IR) spectroscopy. The former identifies the anatomical parameters of the developing tumor, whilst the latter identifies its biochemical features, both at the micron scale. To implement this diagnostic method, an animal model that mimics human melanoma was developed. The results of this investigation show that using high-frequency (&gt;20 MHz) acoustic microscopy in conjunction with spectroscopic images provides useful information about distinct features of melanoma tumors\u2019 3D structures. The structures and cytoarchitecture of the tumors were assessed using conventional histology, and their malignant nature was confirmed using histological and immumohistochemical analysis. The proposed approach may provide an invaluable tool in diagnostic dermatology, as it is noninvasive and produces highly detailed and accurate data about the early appearance and development of melanoma tumors.<\/jats:p>","DOI":"10.3390\/cancers17152599","type":"journal-article","created":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T08:09:35Z","timestamp":1754640575000},"page":"2599","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Diagnostic System for Early In Situ Melanoma Detection Using Acoustic Microscopy and Infrared Spectroscopic Mapping Imaging"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9847-6354","authenticated-orcid":false,"given":"Georgios th","family":"Karagiannis","sequence":"first","affiliation":[{"name":"\u201cORMYLIA\u201d Foundation, 63071 Ormylia, Greece"},{"name":"Diagnosis Multisystems, 63200 Nea Moudania, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2280-4398","authenticated-orcid":false,"given":"Ioannis","family":"Grivas","sequence":"additional","affiliation":[{"name":"Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6610-5847","authenticated-orcid":false,"given":"Anastasia","family":"Tsingotjidou","sequence":"additional","affiliation":[{"name":"Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgios","family":"Apostolidis","sequence":"additional","affiliation":[{"name":"\u201cORMYLIA\u201d Foundation, 63071 Ormylia, Greece"},{"name":"Diagnosis Multisystems, 63200 Nea Moudania, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eirini","family":"Tsardaka","sequence":"additional","affiliation":[{"name":"\u201cORMYLIA\u201d Foundation, 63071 Ormylia, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioanna","family":"Dori","sequence":"additional","affiliation":[{"name":"Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyriaki-Nefeli","family":"Poulatsidou","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Neurology and Neuroimmunology, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5204-5273","authenticated-orcid":false,"given":"Ioannis","family":"Tsougos","sequence":"additional","affiliation":[{"name":"Medical School, University of Thessaly, 41334 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2073-6010","authenticated-orcid":false,"given":"Stefan","family":"Wesarg","sequence":"additional","affiliation":[{"name":"Competence Center Visual Healthcare Technologies of Fraunhofer IGD, 64283 Darmstadt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Argyrios","family":"Doumas","sequence":"additional","affiliation":[{"name":"Radiology-Medical Physics and Informatics Sector, Medical School, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiotis","family":"Georgoulias","sequence":"additional","affiliation":[{"name":"Medical School, University of Thessaly, 41334 Larissa, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,7]]},"reference":[{"key":"ref_1","first-page":"17","article-title":"Cancer statistics, 2023","volume":"73","author":"Siegel","year":"2023","journal-title":"CA Cancer J. 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