{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T08:43:17Z","timestamp":1782463397091,"version":"3.54.5"},"reference-count":66,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T00:00:00Z","timestamp":1757030400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Cutaneous T-cell Lymphomas (CTCLs) are a heterogeneous group of non-Hodgkin lymphomas that currently have an incompletely understood pathophysiology and several challenges in both diagnosis and management. Single-cell RNA sequencing (scRNA-seq) is a powerful tool that enables the analysis of gene expression at the individual-cell level, revealing cellular heterogeneity and a complex tumor microenvironment. As single-cell RNA sequencing has become increasingly utilized, we aimed to provide an update on recent notable applications of single-cell RNA sequencing in CTCL and their findings. The included studies highlight the intricate network of interactions in the tumor microenvironment that contributes to tumorigenesis. While CTCL is notoriously heterogeneous, our results identify key markers that prove promising for diagnosis, prognostication, and therapeutic targets.<\/jats:p>","DOI":"10.3390\/cancers17172921","type":"journal-article","created":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T14:53:01Z","timestamp":1757083981000},"page":"2921","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An Update on Single-Cell RNA Sequencing in Illuminating Disease Mechanisms of Cutaneous T-Cell Lymphoma"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4535-0082","authenticated-orcid":false,"given":"Sara","family":"Suhl","sequence":"first","affiliation":[{"name":"Vagelos College of Physicians and Surgeons, Columbia University, New York, NY 10032, 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Neoplasms","volume":"36","author":"Alaggio","year":"2022","journal-title":"Leukemia"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1002\/ajh.27735","article-title":"Mycosis Fungoides, S\u00e9zary Syndrome, and Cutaneous B-Cell Lymphomas: 2025 Update on Diagnosis, Risk-Stratification, and Management","volume":"100","author":"Hristov","year":"2025","journal-title":"Am. J. Hematol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1690","DOI":"10.1001\/jamaoncol.2022.3236","article-title":"Incidence Trends of Primary Cutaneous T-Cell Lymphoma in the US From 2000 to 2018: A SEER Population Data Analysis","volume":"8","author":"Cai","year":"2022","journal-title":"JAMA Oncol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1182\/blood.2019004256","article-title":"Genetic and epigenetic insights into cutaneous T-cell lymphoma","volume":"139","author":"Tensen","year":"2022","journal-title":"Blood"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1182\/blood.2020009655","article-title":"Integrated genomic analyses of cutaneous T-cell lymphomas reveal the molecular bases for disease heterogeneity","volume":"138","author":"Park","year":"2021","journal-title":"Blood"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Bakr, F.S., and Whittaker, S.J. (2022). Advances in the understanding and treatment of Cutaneous T-cell Lymphoma. Front. Oncol., 12.","DOI":"10.3389\/fonc.2022.1043254"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1002\/ajh.26299","article-title":"Cutaneous T-cell lymphomas: 2021 update on diagnosis, risk-stratification, and management","volume":"96","author":"Hristov","year":"2021","journal-title":"Am. J. Hematol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1038\/nmeth.1315","article-title":"mRNA-Seq whole-transcriptome analysis of a single cell","volume":"6","author":"Tang","year":"2009","journal-title":"Nat. Methods"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1126\/science.aad0501","article-title":"Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq","volume":"352","author":"Tirosh","year":"2016","journal-title":"Science"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.1016\/j.ccell.2021.08.011","article-title":"The landscape of tumor cell states and ecosystems in diffuse large B cell lymphoma","volume":"39","author":"Steen","year":"2021","journal-title":"Cancer Cell"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1182\/blood-2018-08-862292","article-title":"Single-cell RNA-Seq of follicular lymphoma reveals malignant B-cell types and coexpression of T-cell immune checkpoints","volume":"133","author":"Andor","year":"2019","journal-title":"Blood"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1158\/2159-8290.CD-19-0680","article-title":"Single-Cell Transcriptome Analysis Reveals Disease-Defining T-cell Subsets in the Tumor Microenvironment of Classic Hodgkin Lymphoma","volume":"10","author":"Aoki","year":"2020","journal-title":"Cancer Discov."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4443","DOI":"10.1158\/1078-0432.CCR-19-0148","article-title":"Single-Cell Lymphocyte Heterogeneity in Advanced Cutaneous T-cell Lymphoma Skin Tumors","volume":"25","author":"Gaydosik","year":"2019","journal-title":"Clin. Cancer Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1038\/s41592-019-0392-0","article-title":"Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells","volume":"16","author":"Mimitou","year":"2019","journal-title":"Nat. Methods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1186\/s12943-021-01419-2","article-title":"Single-cell RNA sequencing reveals markers of disease progression in primary cutaneous T-cell lymphoma","volume":"20","author":"Rindler","year":"2021","journal-title":"Mol. Cancer"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1456","DOI":"10.1182\/blood.2020009346","article-title":"Multimodal single-cell analysis of cutaneous T-cell lymphoma reveals distinct subclonal tissue-dependent signatures","volume":"138","author":"Herrera","year":"2021","journal-title":"Blood"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1111\/bjd.20512","article-title":"Single-cell RNA sequencing profiling in a patient with discordant primary cutaneous B-cell and T-cell lymphoma reveals micromilieu-driven immune skewing","volume":"185","author":"Jonak","year":"2021","journal-title":"Br. J. Dermatol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Li, Z., Wang, H., Dong, R., Man, J., Sun, L., Qian, X., Zhu, X., Cao, P., Yu, Y., and Le, J. (2021). Single-Cell RNA-seq Reveals Characteristics of Malignant Cells and Immune Microenvironment in Subcutaneous Panniculitis-Like T-Cell Lymphoma. Front. Oncol., 11.","DOI":"10.3389\/fonc.2021.611580"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2996","DOI":"10.1158\/1078-0432.CCR-18-3309","article-title":"Single-Cell Profiling of Cutaneous T-Cell Lymphoma Reveals Underlying Heterogeneity Associated with Disease Progression","volume":"25","author":"Borcherding","year":"2019","journal-title":"Clin. Cancer Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1182\/bloodadvances.2018022608","article-title":"Single-cell heterogeneity in S\u00e9zary syndrome","volume":"2","author":"Buus","year":"2018","journal-title":"Blood Adv."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"5586","DOI":"10.1182\/bloodadvances.2022008562","article-title":"S\u00e9zary syndrome originates from heavily mutated hematopoietic progenitors","volume":"7","author":"Harro","year":"2023","journal-title":"Blood Adv."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.jaci.2024.09.004","article-title":"Single-cell sequencing delineates T-cell clonality and pathogenesis of the parapsoriasis disease group","volume":"155","author":"Alkon","year":"2025","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1182\/bloodadvances.2021005991","article-title":"Single-cell analysis of S\u00e9zary syndrome reveals novel markers and shifting gene profiles associated with treatment","volume":"7","author":"Borcherding","year":"2023","journal-title":"Blood Adv."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1182\/bloodadvances.2022008168","article-title":"Integrated transcriptome and trajectory analysis of cutaneous T-cell lymphoma identifies putative precancer populations","volume":"7","author":"Ren","year":"2023","journal-title":"Blood Adv."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1016\/j.jid.2023.10.018","article-title":"Deciphering Tumor Cell Evolution in Cutaneous T-Cell Lymphomas: Distinct Differentiation Trajectories in Mycosis Fungoides and S\u00e9zary Syndrome","volume":"144","author":"Jiang","year":"2024","journal-title":"J. Investig. Dermatol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e14992","DOI":"10.1111\/exd.14992","article-title":"Single-cell RNA sequencing reveals the underlying mechanism of folliculotropism in folliculotropic mycosis fungoides","volume":"33","author":"Shi","year":"2024","journal-title":"Exp. Dermatol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Kwantwi, L.B., Rosen, S.T., and Querfeld, C. (2024). The Tumor Microenvironment as a Therapeutic Target in Cutaneous T Cell Lymphoma. Cancers, 16.","DOI":"10.3390\/cancers16193368"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1016\/j.jid.2022.07.037","article-title":"Exploring the Nonlymphocytic Cutaneous Microenvironment in Advanced Cutaneous T-Cell Lymphomas using Single-Cell RNA Sequencing","volume":"143","author":"Calugareanu","year":"2023","journal-title":"J. Investig. Dermatol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e170015","DOI":"10.1172\/jci.insight.170015","article-title":"The mycosis fungoides cutaneous microenvironment shapes dysfunctional cell trafficking, antitumor immunity, matrix interactions, and angiogenesis","volume":"8","author":"Gaydosik","year":"2023","journal-title":"JCI Insight"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1038\/s41419-022-05323-5","article-title":"Single-cell analyses reveal novel molecular signatures and pathogenesis in cutaneous T cell lymphoma","volume":"13","author":"Xue","year":"2022","journal-title":"Cell Death Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1016\/j.jaci.2013.03.015","article-title":"Mechanisms underlying helper T-cell plasticity: Implications for immune-mediated disease","volume":"131","author":"Hirahara","year":"2013","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1093\/bjd\/ljae313","article-title":"Single-cell RNA sequencing comparison of CD4+, CD8+ and T-cell receptor \u03b3\u03b4+ cutaneous T-cell lymphomas reveals subset-specific molecular phenotypes","volume":"192","author":"Chennareddy","year":"2025","journal-title":"Br. J. Dermatol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"892","DOI":"10.1016\/j.jaci.2024.11.037","article-title":"Single-cell RNA sequencing of chronic idiopathic erythroderma defines disease-specific markers","volume":"155","author":"Chennareddy","year":"2025","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"100681","DOI":"10.1016\/j.modpat.2024.100681","article-title":"Spatially Resolved Single-Cell Transcriptome Analysis of Mycosis Fungoides Reveals Distinct Biomarkers GNLY and FYB1 Compared With Psoriasis and Chronic Spongiotic Dermatitis","volume":"38","author":"Jung","year":"2025","journal-title":"Mod. Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"10594","DOI":"10.1038\/s41467-024-54354-3","article-title":"CDK9 recruits HUWE1 to degrade RAR\u03b1 and offers therapeutic opportunities for cutaneous T-cell lymphoma","volume":"15","author":"Luo","year":"2024","journal-title":"Nat. Commun."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Srinivas, N., Peiffer, L., Horny, K., Lei, K.C., Buus, T.B., Kubat, L., Luo, M., Yin, M., Spassova, I., and Sucker, A. (2024). Single-cell RNA and T-cell receptor sequencing unveil mycosis fungoides heterogeneity and a possible gene signature. Front. Oncol., 14.","DOI":"10.3389\/fonc.2024.1408614"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2320","DOI":"10.1038\/s41590-024-02018-1","article-title":"Cutaneous T cell lymphoma atlas reveals malignant T(H)2 cells supported by a B cell-rich tumor microenvironment","volume":"25","author":"Li","year":"2024","journal-title":"Nat. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1016\/j.jaci.2024.10.028","article-title":"Integrative epidemiology and immunotranscriptomics uncover a risk and potential mechanism for cutaneous lymphoma unmasking or progression with dupilumab therapy","volume":"155","author":"Carey","year":"2025","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Zhao, Y., Li, Y., Wang, P., Zhu, M., Wang, J., Xie, B., Tang, C., Ma, Y., Wang, S., and Jin, S. (2024). The cancer-associated fibroblasts interact with malignant T cells in mycosis fungoides and promote the disease progression. Front. Immunol., 15.","DOI":"10.3389\/fimmu.2024.1474564"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2384","DOI":"10.1182\/bloodadvances.2023010043","article-title":"Unleashed monocytic engagement in S\u00e9zary syndrome during the combination of anti-CCR4 antibody with type I interferon","volume":"8","author":"Jiang","year":"2024","journal-title":"Blood Adv."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"215972","DOI":"10.1016\/j.canlet.2022.215972","article-title":"Single-cell RNA sequencing unveils the communications between malignant T and myeloid cells contributing to tumor growth and immunosuppression in cutaneous T-cell lymphoma","volume":"551","author":"Du","year":"2022","journal-title":"Cancer Lett."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e164793","DOI":"10.1172\/jci.insight.164793","article-title":"Hyperprogression of cutaneous T cell lymphoma after anti-PD-1 treatment","volume":"8","author":"Gao","year":"2023","journal-title":"JCI Insight"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Roccuzzo, G., Giordano, S., Fava, P., Pileri, A., Guglielmo, A., Tonella, L., Sanlorenzo, M., Ribero, S., Fierro, M.T., and Quaglino, P. (2021). Immune Check Point Inhibitors in Primary Cutaneous T-Cell Lymphomas: Biologic Rationale, Clinical Results and Future Perspectives. Front. Oncol., 11.","DOI":"10.3389\/fonc.2021.733770"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.1200\/JCO.2015.65.9789","article-title":"Nivolumab in Patients With Relapsed or Refractory Hematologic Malignancy: Preliminary Results of a Phase Ib Study","volume":"34","author":"Lesokhin","year":"2016","journal-title":"J. Clin. Oncol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Narducci, M.G., Tosi, A., Frezzolini, A., Scala, E., Passarelli, F., Bonmassar, L., Monopoli, A., Accetturi, M.P., Cantonetti, M., and Antonini Cappellini, G.C. (2020). Reduction of T Lymphoma Cells and Immunological Invigoration in a Patient Concurrently Affected by Melanoma and Sezary Syndrome Treated With Nivolumab. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.579894"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1200\/JCO.19.01056","article-title":"Pembrolizumab in Relapsed and Refractory Mycosis Fungoides and S\u00e9zary Syndrome: A Multicenter Phase II Study","volume":"38","author":"Khodadoust","year":"2020","journal-title":"J. Clin. Oncol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"8049","DOI":"10.1200\/JCO.2020.38.15_suppl.8049","article-title":"The Integration of PD1 blockade with epigenetic therapy is highly active and safe in heavily treated patients with T-cell lymphoma (PTCL) and cutaneous T-cell lymphoma (CTCL)","volume":"38","author":"Marchi","year":"2020","journal-title":"J. Clin. Oncol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2247","DOI":"10.1182\/bloodadvances.2024014655","article-title":"Phase 1 trial of durvalumab (anti-PD-L1) combined with lenalidomide in relapsed\/refractory cutaneous T-cell lymphoma","volume":"9","author":"Querfeld","year":"2025","journal-title":"Blood Adv."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e808","DOI":"10.1016\/S2352-3026(21)00271-4","article-title":"Intralesional TTI-621, a novel biologic targeting the innate immune checkpoint CD47, in patients with relapsed or refractory mycosis fungoides or S\u00e9zary syndrome; syndrome: A multicentre, phase 1 study","volume":"8","author":"Querfeld","year":"2021","journal-title":"Lancet Haematol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1093\/bjd\/ljaf001","article-title":"Preclinical in vitro and in vivo evidence for targeting CD74 as an effective treatment strategy for cutaneous T-cell lymphomas","volume":"192","author":"Costanza","year":"2025","journal-title":"Br. J. Dermatol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2115197","DOI":"10.1080\/2162402X.2022.2115197","article-title":"Single-cell RNA-sequencing reveals predictive features of response to pembrolizumab in S\u00e9zary syndrome","volume":"11","author":"Su","year":"2022","journal-title":"OncoImmunology"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"S53","DOI":"10.1016\/j.jid.2023.03.315","article-title":"311 Single-cell transcriptomics reveals distinct molecular programs in folliculotropic mycosis fungoides","volume":"143","author":"Qin","year":"2023","journal-title":"J. Investig. Dermatol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1182\/blood-2024-210072","article-title":"Single Cell Atlas of Cutaneous T Cell Lymphomas Reveals XPO1 Dependency","volume":"144","author":"Wang","year":"2024","journal-title":"Blood"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"S133","DOI":"10.1016\/j.jid.2025.06.780","article-title":"0767 Characterizing malignant T cells in CTCL to inform personalized therapy: A scRNAseq study","volume":"145","author":"Childs","year":"2025","journal-title":"J. Investig. Dermatol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"AB37","DOI":"10.1016\/j.jaad.2025.05.154","article-title":"64086 Dissecting Malignant T-Cell Behavior in CTCL: A Single-Cell RNA Sequencing Comparison with Benign T Cells in the Immune Microenvironment","volume":"93","author":"Childs","year":"2025","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"6268","DOI":"10.1182\/blood-2024-206387","article-title":"Single-Cell Insights into Cellular Heterogeneity and Immune Dynamics in Cutaneous T-Cell Lymphoma","volume":"144","author":"Childs","year":"2024","journal-title":"Blood"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"S18","DOI":"10.1016\/j.jid.2024.06.115","article-title":"099 Single-cell RNA sequencing reveals differential gene expression of cancer-associated fibroblast markers in mycosis fungoides by stage and race","volume":"144","author":"Johnson","year":"2024","journal-title":"J. Investig. Dermatol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Rassek, K., and I\u017cykowska, K. (2020). Single-Cell Heterogeneity of Cutaneous T-Cell Lymphomas Revealed Using RNA-Seq Technologies. Cancers, 12.","DOI":"10.3390\/cancers12082129"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1186\/s13073-017-0467-4","article-title":"A practical guide to single-cell RNA-sequencing for biomedical research and clinical applications","volume":"9","author":"Haque","year":"2017","journal-title":"Genome Med."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Luo, Y., De Gruijl, F.R., Vermeer, M.H., and Tensen, C.P. (2024). \u201cNext top\u201d mouse models advancing CTCL research. Front. Cell Dev. Biol., 12.","DOI":"10.3389\/fcell.2024.1372881"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1038\/s41592-020-01033-y","article-title":"Method of the Year: Spatially resolved transcriptomics","volume":"18","author":"Marx","year":"2021","journal-title":"Nat. Methods"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Deconinck, L., Cannoodt, R., Saelens, W., Deplancke, B., and Saeys, Y. (2021). Recent advances in trajectory inference from single-cell omics data. Curr. Opin. Syst. Biol., 27.","DOI":"10.1016\/j.coisb.2021.05.005"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"L\u00e4hnemann, D., K\u00f6ster, J., Szczurek, E., McCarthy, D.J., Hicks, S.C., Robinson, M.D., Vallejos, C.A., Campbell, K.R., Beerenwinkel, N., and Mahfouz, A. (2020). Eleven grand challenges in single-cell data science. Genome Biol., 21.","DOI":"10.1186\/s13059-020-1926-6"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1038\/s41467-022-28799-3","article-title":"Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma","volume":"13","author":"Liu","year":"2022","journal-title":"Nat. Commun."}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/17\/17\/2921\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:40:21Z","timestamp":1760035221000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/17\/17\/2921"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,5]]},"references-count":66,"journal-issue":{"issue":"17","published-online":{"date-parts":[[2025,9]]}},"alternative-id":["cancers17172921"],"URL":"https:\/\/doi.org\/10.3390\/cancers17172921","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,5]]}}}