{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T08:11:50Z","timestamp":1760602310783,"version":"build-2065373602"},"reference-count":58,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T00:00:00Z","timestamp":1760400000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Canadian Institutes of Health Research and King Fahad Specialist Hospital-Dammam"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background\/Objectives: Type II ovarian cancer, including high-grade serous carcinoma (HGSC), is genetically unstable and exhibits frequent mutations in the tumor suppressor genes. Mutations of TP53 and BRCA1 genes have been associated with HGSC, which has been suggested as a subtype that arises from the fallopian tube lesion called serous tubal intraepithelial carcinoma (STIC). Although TP53 and BRCA1 genes are well-known tumor suppressor genes, the actual effects of TP53 and BRCA1 mutations in enhancing the development of ovarian cancer initiated from STIC are poorly understood. Methods: In this study, we knocked out Trp53 and Brca-1 in epithelial cell clones derived from mice fallopian tube tissues (known as oviducts) and investigated the potential involvement of these two mutations in inducing cancer stem-like cells as cancer-initiating cells. Results: We have shown that the knockout of Trp53 induced oviduct cells to undergo EMT and acquire stem cell characteristics. Conclusions:\u00a0Trp53 mutation may induce the early stage of precursor lesions formation at the distal end of the oviducts.<\/jats:p>","DOI":"10.3390\/cancers17203317","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T06:06:32Z","timestamp":1760508392000},"page":"3317","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["P53 Mutation Induces Epithelial-to-Mesenchymal Transition (EMT) Associated with Stem Cell Properties and Tumorigenesis in Fallopian Tube Cells"],"prefix":"10.3390","volume":"17","author":[{"given":"Kholoud","family":"Alwosaibai","sequence":"first","affiliation":[{"name":"Biomedical Research Department, Research Center, King Fahad Specialist Hospital-Dammam, Eastern Health Cluster, Dammam 32253, Saudi Arabia"},{"name":"Departments of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada"},{"name":"Cancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7644-7189","authenticated-orcid":false,"given":"Barbara C.","family":"Vanderhyden","sequence":"additional","affiliation":[{"name":"Departments of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada"},{"name":"Cancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada"},{"name":"Department of Obstetrics and Gynecology, University of Ottawa, Ottawa, ON K1H 8L6, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4046-6211","authenticated-orcid":false,"given":"Fatimah A.","family":"Alsaffar","sequence":"additional","affiliation":[{"name":"Medical Laboratory Science Department, AlGhad College for Applied Medical Sciences, Dammam 32423, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salma","family":"Alamri","sequence":"additional","affiliation":[{"name":"Biomedical Research Department, Research Center, King Fahad Specialist Hospital-Dammam, Eastern Health Cluster, Dammam 32253, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdulaziz A.","family":"Almotlak","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Imam Abdulrahman bin Faisal University, Dammam 31441, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.mce.2009.12.025","article-title":"The Mullerian HOXA10 Gene Promotes Growth of Ovarian Surface Epithelial Cells by Stimulating Epithelial\u2013Stromal Interactions","volume":"317","author":"Ko","year":"2010","journal-title":"Mol. 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