{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T01:38:22Z","timestamp":1787621902044,"version":"build-2736575974"},"reference-count":88,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004271","name":"\u201cSapienza\u201d University of Rome","doi-asserted-by":"crossref","award":["RM123188E89E940B"],"award-info":[{"award-number":["RM123188E89E940B"]}],"id":[{"id":"10.13039\/501100004271","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background\/Objectives: The genitourinary microbiome and metabolome may contribute to prostate cancer (PC) biology, but evidence remains limited. This pilot study characterizes the urinary microbiota and volatilome in men with PC and investigates microbial and viral DNA in prostate tissue, comparing findings with benign prostatic hyperplasia (BPH). Methods: We prospectively enrolled 21 non-metastatic PC patients undergoing radical prostatectomy and 17 BPH controls. Lesional and non-lesional prostate tissues and urine were collected from PC patients, as well as urine samples from BPH participants. DNA samples were tested for sexually transmitted pathogens by multiplex real-time PCR. Urine and prostate tissue were analyzed for human polyomaviruses (JCPyV, BKPyV, MCPyV) by qPCR, bacterial profiles via 16S rRNA gene sequencing, and urinary volatile organic metabolites (VOMs) using HS-SPME\/GC-MS. Microbial and metabolic profiles were compared, and taxa\u2013metabolites were assessed. Results: JCPyV and BKPyV were detected in urine and tissue from PC patients; MCPyV was detected only in tissue, at low frequency. In BPH, viral prevalence was lower and MCPyV was absent. JCPyV\/BKPyV co-infection was common in cancer. No sexually transmitted pathogen emerged. PC patients showed greater urinary microbial diversity and five enriched genera, along with specific metabolic pathways. 36 urinary VOMs were identified, with 14 differing significantly, with positive correlations between PC-associated genera and metabolites. In contrast, prostate tissue was low-biomass, dominated by Pseudomonas, and showed no significant differences between lesional and non-lesional areas. Conclusions: This preliminary, hypothesis-generating study indicates that urinary, rather than tissue, microbial and volatilome signatures show clearer differences between PC and BPH. These findings suggest possible microbiota\u2013metabolite interactions in PC but require validation in larger cohorts.<\/jats:p>","DOI":"10.3390\/cancers17233841","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T10:34:01Z","timestamp":1764758041000},"page":"3841","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genitourinary Microbiome and Volatilome: A Pilot Study in Patients with Prostatic Adenocarcinoma Submitted to Radical Prostatectomy"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5598-4367","authenticated-orcid":false,"given":"Layla","family":"Musleh","sequence":"first","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Microbiology Section \u201cSapienza\u201d University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy"},{"name":"Department of Pediatric Surgery, San Camillo-Forlanini Hospital, Circonvallazione Gianicolense 87, 00152 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0678-5537","authenticated-orcid":false,"given":"Sara","family":"Passerini","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Microbiology Section \u201cSapienza\u201d University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9067-6343","authenticated-orcid":false,"given":"Francesca","family":"Brunetti","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Microbiology Section \u201cSapienza\u201d University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5866-4529","authenticated-orcid":false,"given":"Linda","family":"Maurizi","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Microbiology Section \u201cSapienza\u201d University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Bevilacqua","sequence":"additional","affiliation":[{"name":"Department of Maternal-Infant and Urologic Sciences, \u201cSapienza\u201d University of Rome, Viale Policlinico 155, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1949-9100","authenticated-orcid":false,"given":"Lorenzo","family":"Santodirocco","sequence":"additional","affiliation":[{"name":"Department of Maternal-Infant and Urologic Sciences, \u201cSapienza\u201d University of Rome, Viale Policlinico 155, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0502-7613","authenticated-orcid":false,"given":"Beatrice","family":"Sciarra","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technologies of Drug, \u201cSapienza\u201d University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Moriconi","sequence":"additional","affiliation":[{"name":"Department of Maternal-Infant and Urologic Sciences, \u201cSapienza\u201d University of Rome, Viale Policlinico 155, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Fraschetti","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technologies of Drug, \u201cSapienza\u201d University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonello","family":"Filippi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Technologies of Drug, \u201cSapienza\u201d University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0753-6003","authenticated-orcid":false,"given":"Maria Pia","family":"Conte","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Microbiology Section \u201cSapienza\u201d University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5723-8886","authenticated-orcid":false,"given":"Valeria","family":"Pietropaolo","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Microbiology Section \u201cSapienza\u201d University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0197-0485","authenticated-orcid":false,"given":"Marisa","family":"Di Pietro","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Microbiology Section \u201cSapienza\u201d University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2730-8588","authenticated-orcid":false,"given":"Simone","family":"Filardo","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Microbiology Section \u201cSapienza\u201d University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7899-8056","authenticated-orcid":false,"given":"Alessandro","family":"Sciarra","sequence":"additional","affiliation":[{"name":"Department of Maternal-Infant and Urologic Sciences, \u201cSapienza\u201d University of Rome, Viale Policlinico 155, 00161 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7290-3813","authenticated-orcid":false,"given":"Catia","family":"Longhi","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Microbiology Section \u201cSapienza\u201d University of Rome, P. le Aldo Moro 5, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"ref_1","unstructured":"(2025, July 29). Prostate Cancer Burden in EU-27. Available online: https:\/\/ecis.jrc.ec.europa.eu\/sites\/default\/files\/2023-12\/prostate_cancer_En-Nov_2021.pdf."},{"key":"ref_2","unstructured":"(2025, September 01). World Cancer Research Fund. Available online: https:\/\/www.wcrf.org\/cancer-trends\/prostate-cancer-statistics."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1001\/jama.2015.6036","article-title":"Trends in Management for Patients With Localized Prostate Cancer, 1990\u20132013","volume":"314","author":"Cooperberg","year":"2015","journal-title":"JAMA"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1105","DOI":"10.1101\/gad.315739.118","article-title":"Genetics and Biology of Prostate Cancer","volume":"32","author":"Wang","year":"2018","journal-title":"Genes. Dev."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1038\/nrclinonc.2014.68","article-title":"High-Risk Prostate Cancer-Classification and Therapy","volume":"11","author":"Chang","year":"2014","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/j.mcna.2017.03.009","article-title":"Prevention of Prostate Cancer Morbidity and Mortality: Primary Prevention and Early Detection","volume":"101","author":"Barry","year":"2017","journal-title":"Med. Clin. N. Am."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.2217\/fon-2020-0571","article-title":"The Pivotal Role of TMPRSS2 in Coronavirus Disease 2019 and Prostate Cancer","volume":"16","author":"Mollica","year":"2020","journal-title":"Future Oncol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Shrestha, E., Coulter, J.B., Guzman, W., Ozbek, B., Hess, M.M., Mummert, L., Ernst, S.E., Maynard, J.P., Meeker, A.K., and Heaphy, C.M. (2021). Oncogenic Gene Fusions in Nonneoplastic Precursors as Evidence That Bacterial Infection Can Initiate Prostate Cancer. Proc. Natl. Acad. Sci. USA, 118.","DOI":"10.1073\/pnas.2018976118"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1186\/s13027-022-00427-1","article-title":"Multiple Pathogens and Prostate Cancer","volume":"17","author":"Lawson","year":"2022","journal-title":"Infect. Agent. Cancer"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Abumsimir, B., Almahasneh, I., Kasmi, Y., Hammou, R.A., and Ennaji, M.M. Chapter 15\u2014Prostate Cancer and Viral Infections: Epidemiological and Clinical Indications. Oncogenic Viruses Volume 1: Fundamentals of Oncoviruses 2023, Elsevier.","DOI":"10.1016\/B978-0-12-824152-3.00015-9"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Tsydenova, I.A., Ibragimova, M.K., Tsyganov, M.M., and Litviakov, N.V. (2023). Human Papillomavirus and Prostate Cancer: Systematic Review and Meta-Analysis. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-43767-7"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1080\/01635581.2024.2399868","article-title":"Unveiling the Impact of Epstein-Barr Virus on the Risk of Prostate Cancer: A Mendelian Randomization Study","volume":"77","author":"Liao","year":"2025","journal-title":"Nutr. Cancer"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Todorova, E., Kavrakova, A., Derimachkovski, G., Georgieva, B., Odzhakov, F., Bachurska, S., Terziev, I., Boyadzhieva, M.-E., Valkov, T., and Popov, E. (2025). Human Herpes Virus Genotype and Immunological Gene Expression Profile in Prostate Cancer with Prominent Inflammation. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26104945"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1002\/pros.10157","article-title":"Detection of Human Polyomaviruses and Papillomaviruses in Prostatic Tissue Reveals the Prostate as a Habitat for Multiple Viral Infections","volume":"53","author":"Zambrano","year":"2002","journal-title":"Prostate"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Shen, C., Tung, C., Chao, C., Jou, Y., Huang, S., Meng, M., Chang, D., and Chen, P. (2021). The Differential Presence of Human Polyomaviruses, JCPyV and BKPyV, in Prostate Cancer and Benign Prostate Hypertrophy Tissues. BMC Cancer, 21.","DOI":"10.1186\/s12885-021-08862-w"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1126\/science.1152586","article-title":"Clonal Integration of a Polyomavirus in Human Merkel Cell Carcinoma","volume":"319","author":"Feng","year":"2008","journal-title":"Science"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Prado, J.C.M., Monezi, T.A., Amorim, A.T., Lino, V., Paladino, A., and Boccardo, E. (2018). Human Polyomaviruses and Cancer: An Overview. Clinics, 73.","DOI":"10.6061\/clinics\/2018\/e558s"},{"key":"ref_18","first-page":"189","article-title":"High Frequency of JCV DNA Detection in Prostate Cancer Tissues","volume":"12","author":"Anzivino","year":"2015","journal-title":"Cancer Genom. Proteom."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s00430-013-0304-3","article-title":"Evidence Supporting the Association of Polyomavirus BK Genome with Prostate Cancer","volume":"202","author":"Delbue","year":"2013","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1177\/172460080702200402","article-title":"Prevalence of BK Virus and Human Papillomavirus in Human Prostate Cancer","volume":"22","author":"Balis","year":"2007","journal-title":"Int. J. Biol. Markers"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2705","DOI":"10.1128\/JVI.02461-07","article-title":"BK Virus as a Cofactor in the Etiology of Prostate Cancer in Its Early Stages","volume":"82","author":"Das","year":"2008","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2100","DOI":"10.1002\/jmv.21312","article-title":"p53 Gene Mutational Rate, Gleason Score, and BK Virus Infection in Prostate Adenocarcinoma: Is There a Correlation?","volume":"80","author":"Russo","year":"2008","journal-title":"J. Med. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1111\/apm.13019","article-title":"Merkel Cell Polyomavirus and Non-Merkel Cell Carcinomas: Guilty or Circumstantial Evidence?","volume":"128","author":"Csoboz","year":"2020","journal-title":"APMIS"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.jcv.2008.11.008","article-title":"Merkel Cell Polyomavirus Is Not Detected in Prostate Cancers, Surrounding Stroma, or Benign Prostate Controls","volume":"44","author":"Bluemn","year":"2009","journal-title":"J. Clin. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Massari, F., Mollica, V., Di Nunno, V., Gatto, L., Santoni, M., Scarpelli, M., Cimadamore, A., Lopez-Beltran, A., Cheng, L., and Battelli, N. (2019). The Human Microbiota and Prostate Cancer: Friend or Foe?. Cancers, 11.","DOI":"10.3390\/cancers11040459"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1136\/gutjnl-2017-314205","article-title":"Gastric Microbial Community Profiling Reveals a Dysbiotic Cancer-Associated Microbiota","volume":"67","author":"Ferreira","year":"2018","journal-title":"Gut"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Oc\u00e1riz-D\u00edez, M., Cruellas, M., Gasc\u00f3n, M., Lastra, R., Mart\u00ednez-Lostao, L., Ram\u00edrez-Labrada, A., Pa\u00f1o, J.R., Sesma, A., Torres, I., and Yubero, A. (2020). Microbiota and Lung Cancer. Opportunities and Challenges for Improving Immunotherapy Efficacy. Front. Oncol., 10.","DOI":"10.3389\/fonc.2020.568939"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Visconti, A., Le Roy, C.I., Rosa, F., Rossi, N., Martin, T.C., Mohney, R.P., Li, W., de Rinaldis, E., Bell, J.T., and Venter, J.C. (2019). Interplay between the Human Gut Microbiome and Host Metabolism. Nat. Commun., 10.","DOI":"10.1038\/s41467-019-12476-z"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lima, A.R., Pinto, J., Amaro, F., Bastos, M.d.L., Carvalho, M., and Guedes de Pinho, P. (2021). Advances and Perspectives in Prostate Cancer Biomarker Discovery in the Last 5 Years through Tissue and Urine Metabolomics. Metabolites, 11.","DOI":"10.3390\/metabo11030181"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/s41585-019-0185-3","article-title":"Cancer Metabolomic Markers in Urine: Evidence, Techniques and Recommendations","volume":"16","author":"Dinges","year":"2019","journal-title":"Nat. Rev. Urol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"995","DOI":"10.1080\/14789450.2021.2011225","article-title":"Noninvasive Urine Metabolomics of Prostate Cancer and Its Therapeutic Approaches: A Current Scenario and Future Perspective","volume":"18","author":"Kumar","year":"2021","journal-title":"Expert. Rev. Proteom."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1136\/gutjnl-2015-309990","article-title":"The Gut Microbiota and Host Health: A New Clinical Frontier","volume":"65","author":"Marchesi","year":"2016","journal-title":"Gut"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.radonc.2020.01.011","article-title":"Fecal Microbiota Transplantation: A Promising Treatment for Radiation Enteritis?","volume":"143","author":"Ding","year":"2020","journal-title":"Radiother. Oncol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e87","DOI":"10.1097\/PAS.0000000000001497","article-title":"The 2019 International Society of Urological Pathology (ISUP) Consensus Conference on Grading of Prostatic Carcinoma","volume":"44","author":"Grignon","year":"2020","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Delbue, S., Franciotta, D., Giannella, S., Dolci, M., Signorini, L., Ticozzi, R., D\u2019Alessandro, S., Campisciano, G., Comar, M., and Ferrante, P. (2019). Human Polyomaviruses in the Cerebrospinal Fluid of Neurological Patients. Microorganisms, 8.","DOI":"10.3390\/microorganisms8010016"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Passerini, S., Babini, G., Merenda, E., Carletti, R., Scribano, D., Rosa, L., Conte, A.L., Moens, U., Ottolenghi, L., and Romeo, U. (2024). Merkel Cell Polyomavirus in the Context of Oral Squamous Cell Carcinoma and Oral Potentially Malignant Disorders. Biomedicines, 12.","DOI":"10.3390\/biomedicines12040709"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and Clustering Orders of Magnitude Faster than BLAST","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"10","DOI":"10.14806\/ej.17.1.200","article-title":"Cutadapt Removes Adapter Sequences from High-Throughput Sequencing Reads","volume":"17","author":"Martin","year":"2011","journal-title":"EMBnet J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, Interactive, Scalable and Extensible Microbiome Data Science Using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: High-Resolution Sample Inference from Illumina Amplicon Data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1038\/s41587-020-0548-6","article-title":"PICRUSt2 for Prediction of Metagenome Functions","volume":"38","author":"Douglas","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1993","DOI":"10.1016\/j.ejca.2014.04.011","article-title":"Analysis of Volatile Human Urinary Metabolome by Solid-Phase Microextraction in Combination with Gas Chromatography-Mass Spectrometry for Biomarker Discovery: Application in a Pilot Study to Discriminate Patients with Renal Cell Carcinoma","volume":"50","author":"Monteiro","year":"2014","journal-title":"Eur. J. Cancer"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5035","DOI":"10.1021\/ac300698c","article-title":"XCMS Online: A Web-Based Platform to Process Untargeted Metabolomic Data","volume":"84","author":"Tautenhahn","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_45","unstructured":"(2025, September 04). ALDEx2. Available online: http:\/\/bioconductor.org\/packages\/ALDEx2\/."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.eururo.2017.03.029","article-title":"The Microbiome of the Prostate Tumor Microenvironment","volume":"72","author":"Cavarretta","year":"2017","journal-title":"Eur. Urol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Feng, Y., Ramnarine, V.R., Bell, R., Volik, S., Davicioni, E., Hayes, V.M., Ren, S., and Collins, C.C. (2019). Metagenomic and Metatranscriptomic Analysis of Human Prostate Microbiota from Patients with Prostate Cancer. BMC Genom., 20.","DOI":"10.1186\/s12864-019-5457-z"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Gon\u00e7alves, M.F.M., Pina-Vaz, T., Fernandes, \u00c2.R., Miranda, I.M., Silva, C.M., Rodrigues, A.G., and Lisboa, C. (2023). Microbiota of Urine, Glans and Prostate Biopsies in Patients with Prostate Cancer Reveals a Dysbiosis in the Genitourinary System. Cancers, 15.","DOI":"10.3390\/cancers15051423"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"D\u2019Antonio, D.L., Marchetti, S., Pignatelli, P., Piattelli, A., and Curia, M.C. (2022). The Oncobiome in Gastroenteric and Genitourinary Cancers. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23179664"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Li, J., Li, Y., Zhou, L., Li, H., Wan, T., Tang, J., Zhou, L., Xie, H., and Wang, L. (2024). Microbiome Analysis Reveals the Inducing Effect of on Prostatic Hyperplasia via Activating NF-\u03baB Signalling. Virulence, 15.","DOI":"10.1080\/21505594.2024.2313410"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1111\/1755-0998.13011","article-title":"Laboratory contamination over time during low-biomass sample analysis","volume":"19","author":"Weyrich","year":"2019","journal-title":"Mol. Ecol. Resour."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Katongole, P., Sande, O.J., Joloba, M., Reynolds, S.J., and Niyonzima, N. (2020). The Human Microbiome and Its Link in Prostate Cancer Risk and Pathogenesis. Infect. Agent. Cancer, 15.","DOI":"10.1186\/s13027-020-00319-2"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Okada, K., Takezawa, K., Tsujimura, G., Imanaka, T., Kuribayashi, S., Ueda, N., Hatano, K., Fukuhara, S., Kiuchi, H., and Fujita, K. (2022). Localization and Potential Role of Prostate Microbiota. Front. Cell Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.1048319"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13027-016-0112-7","article-title":"Characterisation of Microbial Communities within Aggressive Prostate Cancer Tissues","volume":"12","author":"Yow","year":"2017","journal-title":"Infect. Agent. Cancer"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Higuchi, R., Goto, T., Hirotsu, Y., Otake, S., Oyama, T., Amemiya, K., Mochizuki, H., and Omata, M. (2021). Streptococcus australis and Ralstonia pickettii as Major Microbiota in Mesotheliomas. J. Pers. Med., 11.","DOI":"10.3390\/jpm11040297"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Fujita, K., Matsushita, M., De Velasco, M.A., Hatano, K., Minami, T., Nonomura, N., and Uemura, H. (2023). The Gut-Prostate Axis: A New Perspective of Prostate Cancer Biology through the Gut Microbiome. Cancers, 15.","DOI":"10.3390\/cancers15051375"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1016\/j.tibtech.2017.06.014","article-title":"Sphingomonads in Microbe-Assisted Phytoremediation: Tackling Soil Pollution","volume":"35","author":"Sun","year":"2017","journal-title":"Trends Biotechnol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Coradduzza, D., Sanna, A., Di Lorenzo, B., Congiargiu, A., Marra, S., Cossu, M., Tedde, A., De Miglio, M.R., Zinellu, A., and Mangoni, A.A. (2025). Associations between Plasma and Urinary Heavy Metal Concentrations and the Risk of Prostate Cancer. Sci. Rep., 15.","DOI":"10.1038\/s41598-025-97682-0"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Anchidin-Norocel, L., Iatcu, O.C., Lobiuc, A., and Covasa, M. (2025). Heavy Metal-Gut Microbiota Interactions: Probiotics Modulation and Biosensors Detection. Biosensors, 15.","DOI":"10.3390\/bios15030188"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Khalid, T., Aggio, R., White, P., De Lacy Costello, B., Persad, R., Al-Kateb, H., Jones, P., Probert, C.S., and Ratcliffe, N. (2015). Urinary Volatile Organic Compounds for the Detection of Prostate Cancer. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0143283"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4904","DOI":"10.3390\/curroncol30050370","article-title":"Differences in the Volatilomic Urinary Biosignature of Prostate Cancer Patients as a Feasibility Study for the Detection of Potential Biomarkers","volume":"30","author":"Riccio","year":"2023","journal-title":"Curr. Oncol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1002\/bco2.423","article-title":"Analysis of Urinary Volatile Organic Compounds for Prostate Cancer Diagnosis: A Systematic Review","volume":"5","author":"Dawson","year":"2024","journal-title":"BJUI Compass"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/s41416-019-0585-4","article-title":"Identification of a Biomarker Panel for Improvement of Prostate Cancer Diagnosis by Volatile Metabolic Profiling of Urine","volume":"121","author":"Lima","year":"2019","journal-title":"Br. J. Cancer"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Berenguer, C.V., Pereira, F., Pereira, J.A.M., and C\u00e2mara, J.S. (2022). Volatilomics: An Emerging and Promising Avenue for the Detection of Potential Prostate Cancer Biomarkers. Cancers, 14.","DOI":"10.3390\/cancers14163982"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"2593","DOI":"10.1038\/bjc.2014.201","article-title":"Aldehyde Dehydrogenase 3A1 Associates with Prostate Tumorigenesis","volume":"110","author":"Yan","year":"2014","journal-title":"Br. J. Cancer"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Xia, J., Li, S., Liu, S., and Zhang, L. (2023). Aldehyde Dehydrogenase in Solid Tumors and Other Diseases: Potential Biomarkers and Therapeutic Targets. MedComm, 4.","DOI":"10.1002\/mco2.195"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jchromb.2015.02.036","article-title":"Comprehensive Analysis of the Lipophilic Reactive Carbonyls Present in Biological Specimens by LC\/ESI-MS\/MS","volume":"988","author":"Tomono","year":"2015","journal-title":"J. Chromatogr. B Analyt Technol. Biomed. Life Sci."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Goertzen, A., Kidane, B., Ahmed, N., and Aliani, M. (2024). Potential Urinary Volatile Organic Compounds as Screening Markers in Cancer\u2014A Review. Front. Oncol., 14.","DOI":"10.3389\/fonc.2024.1448760"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s13530-012-0140-7","article-title":"Gene Expression Profiling of Low Dose Exposure of Saturated Aliphatic Aldehydes in A549 Human Alveolar Epithelial Cells","volume":"4","author":"Lee","year":"2012","journal-title":"Toxicol. Environ. Health Sci."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Garbas, K., Zapa\u0142a, P., Zapa\u0142a, \u0141., and Radziszewski, P. (2021). The Role of Microbial Factors in Prostate Cancer Development-An Up-to-Date Review. J. Clin. Med., 10.","DOI":"10.3390\/jcm10204772"},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Janfaza, S., Khorsand, B., Nikkhah, M., and Zahiri, J. (2019). Digging Deeper into Volatile Organic Compounds Associated with Cancer. Biol. Methods Protoc., 4.","DOI":"10.1093\/biomethods\/bpz014"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Riccio, G., Baroni, S., Urbani, A., and Greco, V. (2022). Mapping of Urinary Volatile Organic Compounds by a Rapid Analytical Method Using Gas Chromatography Coupled to Ion Mobility Spectrometry (GC-IMS). Metabolites, 12.","DOI":"10.3390\/metabo12111072"},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Liu, Q., Fan, Y., Zeng, S., Zhao, Y., Yu, L., Zhao, L., Gao, J., Zhang, X., and Zhang, Y. (2023). Volatile Organic Compounds for Early Detection of Prostate Cancer from Urine. Heliyon, 9.","DOI":"10.1016\/j.heliyon.2023.e16686"},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Balahbib, A., El Omari, N., Hachlafi, N.E., Lakhdar, F., El Menyiy, N., Salhi, N., Mrabti, H.N., Bakrim, S., Zengin, G., and Bouyahya, A. (2021). Health Beneficial and Pharmacological Properties of P-Cymene. Food Chem. Toxicol., 153.","DOI":"10.1016\/j.fct.2021.112259"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"5793","DOI":"10.1158\/1078-0432.CCR-04-2545","article-title":"Cytochrome P450 1B1 Is Overexpressed and Regulated by Hypomethylation in Prostate Cancer","volume":"11","author":"Tokizane","year":"2005","journal-title":"Clin. Cancer Res."},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Aarnoutse, R., Ziemons, J., Penders, J., Rensen, S.S., de Vos-Geelen, J., and Smidt, M.L. (2019). The Clinical Link between Human Intestinal Microbiota and Systemic Cancer Therapy. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20174145"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.urolonc.2024.05.002","article-title":"Matrix Metalloproteinases Targeting in Prostate Cancer","volume":"42","author":"Zhu","year":"2024","journal-title":"Urol. Oncol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"1500","DOI":"10.1136\/ijgc-2020-001341","article-title":"Characterization of Vaginal Microbiota in Chinese Women with Cervical Squamous Intra-Epithelial Neoplasia","volume":"30","author":"Wu","year":"2020","journal-title":"Int. J. Gynecol. Cancer"},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Ma, J., Gnanasekar, A., Lee, A., Li, W.T., Haas, M., Wang-Rodriguez, J., Chang, E.Y., Rajasekaran, M., and Ongkeko, W.M. (2020). Influence of Intratumor Microbiome on Clinical Outcome and Immune Processes in Prostate Cancer. Cancers, 12.","DOI":"10.3390\/cancers12092524"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"4355","DOI":"10.7150\/jca.57049","article-title":"P-Cymene Prevent High-Fat Diet-Associated Colorectal Cancer by Improving the Structure of Intestinal Flora","volume":"12","author":"Jin","year":"2021","journal-title":"J. Cancer"},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Pessione, E. (2012). Lactic Acid Bacteria Contribution to Gut Microbiota Complexity: Lights and Shadows. Front. Cell Infect. Microbiol., 2.","DOI":"10.3389\/fcimb.2012.00086"},{"key":"ref_82","doi-asserted-by":"crossref","unstructured":"O\u2019Callaghan, A., and van Sinderen, D. (2016). Bifidobacteria and Their Role as Members of the Human Gut Microbiota. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00925"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.coviro.2019.05.007","article-title":"Virome and Bacteriome: Two Sides of the Same Coin","volume":"37","author":"Stern","year":"2019","journal-title":"Curr. Opin. Virol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/sj.bjc.6603480","article-title":"No Link between Viral Findings in the Prostate and Subsequent Cancer Development","volume":"96","author":"Bergh","year":"2007","journal-title":"Br. J. Cancer"},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Demey, B., Aubry, A., Descamps, V., Morel, V., Le, M.H.H., Presne, C., Brazier, F., Helle, F., and Brochot, E. (2024). Molecular Epidemiology and Risk Factors Associated with BK and JC Polyomavirus Urinary Shedding after Kidney Allograft. J. Med. Virol., 96.","DOI":"10.1002\/jmv.29742"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1347","DOI":"10.1007\/s00345-018-2536-8","article-title":"Cholecalciferol for the Prophylaxis against Recurrent Urinary Tract Infection among Patients with Benign Prostatic Hyperplasia: A Randomized, Comparative Study","volume":"37","author":"Safwat","year":"2019","journal-title":"World J. Urol."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"487","DOI":"10.2217\/fvl-2019-0050","article-title":"An Overview on Human Polyomaviruses Biology and Related Diseases","volume":"14","author":"Ciotti","year":"2019","journal-title":"Future Virol."},{"key":"ref_88","unstructured":"Kamminga, S. (2022). Polyomaviruses in Blood Donors: Detection, Prevalence and Blood Safety. 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