{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:18:09Z","timestamp":1767961089664,"version":"3.49.0"},"reference-count":47,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EU NextGenerationEU","award":["09105-03-V02-00078"],"award-info":[{"award-number":["09105-03-V02-00078"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background\/Objectives: Matrix metalloproteinase-7 (MMP-7) has been implicated in colorectal cancer (CRC) progression; however, its relationship to disease stage and its suitability as a circulating biomarker remain unclear. This study aimed to determine whether MMP-7 expression and activity differ between benign and malignant colorectal conditions and whether serum MMP-7 levels reflect disease progression. Methods: mRNA MMP-7 expression data and MMP-7 levels have been collected from Gepia, Protein Atlas and UALCAN databases. For the study of patient samples, ELISA, Western blot, and zymography were used. The study included 30 patients with benign findings and 60 patients with colorectal cancer. The Gepia database reported significantly higher MMP-7 levels in patients with CRC. Results: The Protein Atlas and UALCAN highlight a notable difference between benign and malignant colon adenocarcinoma patients. The MMP-7 level in tissue samples from the malignant group, evaluated by Western blot, was approximately 4.5 times higher than in the benign group, and almost 3 times higher in serum samples. Using zymography, patients in the malignant group had MMP-7 activity more than 4x higher than that of patients in the benign group. The ELISA results supported this increase in MMP-7 levels. The average MMP-7 level in the malignant group was 1.2-fold that in benign tissue samples and approximately 3-fold that in serum samples. Notably, significant sex-related differences in serum MMP-7 concentrations were observed, indicating that gender may influence the interpretation of this biomarker. Conclusions: The discordance between stable MMP7 mRNA expression and declining serum MMP-7 protein levels in advanced CRC suggests complex post-transcriptional and post-translational regulation of MMP-7 during disease progression. Although this finding contrasts with much of the existing literature, it should be regarded as novel and hypothesis-generating. These results indicate that serum MMP-7 may reflect early tumor-associated processes rather than late-stage tumor burden, warranting further investigation in larger, stage-stratified and longitudinal cohorts.<\/jats:p>","DOI":"10.3390\/cancers18020214","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T10:35:52Z","timestamp":1767954952000},"page":"214","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi-Platform Detection of MMP-7 in Colorectal Carcinoma"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1600-4116","authenticated-orcid":false,"given":"Ivana","family":"Ve\u010durkovsk\u00e1","sequence":"first","affiliation":[{"name":"Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef \u0160af\u00e1rik University in Ko\u0161ice, Trieda SNP 1, 040 11 Ko\u0161ice, Slovakia"},{"name":"Department of Technical Disciplines in Health Care, Faculty of Health Care, University of Presov, Partiz\u00e1nska 1, 080 01 Pre\u0161ov, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3371-9623","authenticated-orcid":false,"given":"Marek","family":"Stup\u00e1k","sequence":"additional","affiliation":[{"name":"Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef \u0160af\u00e1rik University in Ko\u0161ice, Trieda SNP 1, 040 11 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5479-6444","authenticated-orcid":false,"given":"Jana","family":"Ka\u0165uchov\u00e1","sequence":"additional","affiliation":[{"name":"1st Department of Surgery, Faculty of Medicine, Pavol Jozef \u0160af\u00e1rik University in Ko\u0161ice, Trieda SNP 1, 040 11 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Ro\u0161kovi\u010dov\u00e1","sequence":"additional","affiliation":[{"name":"1st Department of Surgery, Faculty of Medicine, Pavol Jozef \u0160af\u00e1rik University in Ko\u0161ice, Trieda SNP 1, 040 11 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Pavlu\u0161","sequence":"additional","affiliation":[{"name":"Department of Transplant Surgery, F.D. Roosevelt Hospital Bansk\u00e1 Bystrica, N\u00e1m. L. Svobodu 1, 975 17 Bansk\u00e1 Bystrica, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9103-4710","authenticated-orcid":false,"given":"M\u00e1ria","family":"Marekov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef \u0160af\u00e1rik University in Ko\u0161ice, Trieda SNP 1, 040 11 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2786-0620","authenticated-orcid":false,"given":"Jana","family":"Ma\u0161lankov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Medical and Clinical Biochemistry, Faculty of Medicine, Pavol Jozef \u0160af\u00e1rik University in Ko\u0161ice, Trieda SNP 1, 040 11 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1136\/gutjnl-2022-327736","article-title":"Global burden of Colorectal Cancer in 2020 and 2040: Incidence and Mortality estimates from GLOBOCAN","volume":"72","author":"Morgan","year":"2023","journal-title":"Gut"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1038\/s41416-019-0385-x","article-title":"Predictive Values of Colorectal Cancer Alarm Symptoms in the General Population: A Nationwide Cohort Study","volume":"120","author":"Rasmussen","year":"2019","journal-title":"Br. J. Cancer"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Zygulska, A.L., and Pierzchalski, P. (2022). Novel Diagnostic Biomarkers in Colorectal Cancer. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23020852"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4744","DOI":"10.3748\/wjg.v28.i33.4744","article-title":"Regulation of Transforming Growth Factor-\u03b2 signaling as a Therapeutic Approach to Treating Colorectal Cancer","volume":"28","author":"Maslankova","year":"2022","journal-title":"World J. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Morgado-Diaz, J.A. (2022). Colorectal Cancer: An Overview. Gastrointestinal Cancers, Exon Publications.","DOI":"10.36255\/exon-publications-gastrointestinal-cancers"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.semcancer.2018.05.002","article-title":"Molecular Subtyping of Colorectal Cancer: Recent Progress, New Challenges and Emerging Opportunities","volume":"55","author":"Wang","year":"2019","journal-title":"Semin. Cancer Biol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Quintero-Fabi\u00e1n, S., Arreola, R., Becerril-Villanueva, E., Torres-Romero, J.C., Arana-Argaez, V., Lara-Riegos, J., Ramirez-Camacho, M.A., and Alvarez-Sanchez, M.E. (2019). Role of Matrix Metalloproteinases in Angiogenesis and Cancer. Front. Oncol., 9.","DOI":"10.3389\/fonc.2019.01370"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3000605211012570","DOI":"10.1177\/03000605211012570","article-title":"Diagnostic Values of MMP-7, MMP-9, MMP-11, TIMP-1, TIMP-2, CEA, and CA19-9 in Patients with Colorectal Cancer","volume":"49","author":"Huang","year":"2021","journal-title":"J. Int. Med. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1159\/000511765","article-title":"The Behavior of MMP-2, MMP-7, MMP-9, and Their Inhibitors TIMP-1 and TIMP-2 in Adenoma-Colorectal Cancer Sequence","volume":"39","author":"Hritz","year":"2021","journal-title":"Dig. Dis."},{"key":"ref_10","first-page":"1671","article-title":"Prognostic Significance of Abnormal Matrix Collagen Remodeling in Colorectal Cancer Based on Histologic and Bioinformatics Analysis","volume":"44","author":"Liang","year":"2020","journal-title":"Oncol. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e174545","DOI":"10.1172\/JCI174545","article-title":"Tissue-specific Reprogramming Leads to Angiogenic Neutrophil Specialization and Tumor Vascularization in Colorectal Cancer","volume":"134","author":"Bui","year":"2024","journal-title":"J. Clin. Investig."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1023\/A:1025867130437","article-title":"Role of Matrix Metalloproteinases (MMPs) in Colorectal Cancer","volume":"23","author":"Zucker","year":"2004","journal-title":"Cancer Metastasis Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"366","DOI":"10.3390\/cancers6010366","article-title":"The Role of Matrix Metalloproteinases in Colorectal Cancer","volume":"6","author":"Said","year":"2014","journal-title":"Cancers"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.clinbiochem.2021.03.003","article-title":"Roles of Matrix Metalloproteinase-7 (MMP-7) in Cancer","volume":"92","author":"Liao","year":"2021","journal-title":"Clin. Biochem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"149","DOI":"10.3109\/03008200109014256","article-title":"Fluorescence polarization assay and SDS-PAGE confirms matrilysin degrades fibronectin and collagen IV whereas gelatinase A degrades collagen IV but not fibronectin","volume":"42","author":"Kraft","year":"2001","journal-title":"Connect. Tissue Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1242\/jcs.114.1.111","article-title":"Release of an invasion promoter E-cadherin fragment by matrilysin and stromelysin-1","volume":"114","author":"Fingleton","year":"2001","journal-title":"J. Cell Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.1016\/S0002-9440(10)64318-0","article-title":"Matrilysin (matrix metalloproteinase-7) mediates E-cadherin ectodomain shedding in injured lung epithelium","volume":"162","author":"McGuire","year":"2003","journal-title":"Am. J. Pathol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"11228","DOI":"10.1158\/0008-5472.CAN-06-1187","article-title":"Matrilysin 1 influences colon carcinoma cell migration by cleavage of the laminin-5 beta3 chain","volume":"66","author":"Remy","year":"2006","journal-title":"Cancer Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.biochi.2010.09.011","article-title":"Insights into the degradation of human elastin by matrilysin-1","volume":"93","author":"Heinz","year":"2011","journal-title":"Biochimie"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1177\/153537020623100103","article-title":"Role of matrix metalloproteinase-7 (matrilysin) in human cancer invasion, apoptosis, growth, and angiogenesis","volume":"231","author":"Ii","year":"2006","journal-title":"Exp. Biol. Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1111\/bju.14415","article-title":"Matrix metalloproteinase 7, soluble Fas and Fas ligand serum levels for predicting docetaxel resistance and survival in castration-resistant prostate cancer","volume":"122","author":"Szarvas","year":"2018","journal-title":"BJU Int."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Cabral-Pacheco, G.A., Garza-Veloz, I., Castruita-De la Rosa, C., Ramirez-Acuna, J.M., Perez-Romero, B.A., Guerrero-Rodriguez, J.F., Martinez-Avila, N., and Martinez-Fierro, M.L. (2020). The Roles of Matrix Metalloproteinases and Their Inhibitors in Human Diseases. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21249739"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Klupp, F., Neumann, L., Kahlert, C., Diers, J., Halama, N., Franz, C., Schmidt, T., Koch, M., Weitz, J., and Schneider, M. (2016). Serum MMP7, MMP10 and MMP12 Level as Negative Prognostic Markers in Colon Cancer Patients. BMC Cancer, 16.","DOI":"10.1186\/s12885-016-2515-7"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1097\/CAD.0000000000000621","article-title":"Celastrol Inhibits Colorectal Cancer Cell Proliferation and Migration Through Suppression of MMP3 and MMP7 by the PI3K\/AKT Signaling Pathway","volume":"29","author":"Bufu","year":"2018","journal-title":"Anticancer Drugs"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Madzharova, E., Kastl, P., Sabino, F., and Auf dem Keller, U. (2019). Post-Translational Modification-Dependent Activity of Matrix Metalloproteinases. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20123077"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Yadav, V., Singh, T., Sharma, D., Garg, V.K., Chakraborty, P., Ghatak, S., and Satapathy, S.R. (2024). Unraveling the Regulatory Role of HuR\/microRNA Axis in Colorectal Cancer Tumorigenesis. Cancers, 16.","DOI":"10.3390\/cancers16183183"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.matbio.2015.02.007","article-title":"Extracellular Regulation of Metalloproteinases","volume":"44-46","author":"Yamamoto","year":"2015","journal-title":"Matrix Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1038\/s41388-017-0021-2","article-title":"FOXC1-induced Non-Canonical WNT5A-MMP7 Signaling Regulates Invasiveness in Triple-Negative Breast Cancer","volume":"37","author":"Han","year":"2018","journal-title":"Oncogene"},{"key":"ref_29","first-page":"1863","article-title":"Clinicopathological Assessment and Quantitative Estimation of the Matrix Metalloproteinases MMP-2 and MMP-7 and the Inhibitors TIMP-1 and TIMP-2 in Colorectal Carcinoma Tissue Samples","volume":"27","author":"Pesta","year":"2007","journal-title":"Anticancer Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1186\/s13000-015-0411-0","article-title":"Evaluation of the Correlation of KAI1\/CD82, CD44, MMP7 and \u03b2-catenin in the Prediction of Prognosis and Metastasis in Colorectal Carcinoma","volume":"10","author":"Wu","year":"2015","journal-title":"Diagn. Pathol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1177\/1724600819866202","article-title":"Serum Levels of TIMP-1 and MMP-7 as Potential Biomarkers in Patients with Metastatic Colorectal Cancer","volume":"34","author":"Vocka","year":"2019","journal-title":"Int. J. Biol. Markers"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1016\/j.clinbiochem.2013.07.015","article-title":"Matrix Metalloproteinase7-181A\/G Polymorphism is Associated with Increased Cancer Risk Among High-Quality Studies: Evidence from a Meta-Analysis","volume":"46","author":"Wu","year":"2013","journal-title":"Clin. Biochem."},{"key":"ref_33","first-page":"207","article-title":"The Contribution of MMP-7 Genotypes to Colorectal Cancer Susceptibility in Taiwan","volume":"15","author":"Yueh","year":"2018","journal-title":"Cancer Genom. Proteom."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"63324","DOI":"10.18632\/oncotarget.11450","article-title":"Sphingosine-1-phosphate Induced Epithelial-Mesenchymal Transition of Hepatocellular Carcinoma via an MMP-7\/ Syndecan-1\/TGF-\u03b2 Autocrine Loop","volume":"7","author":"Zeng","year":"2016","journal-title":"Oncotarget"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2671","DOI":"10.2147\/IJGM.S416363","article-title":"Clinical Significance of MMP7 Levels in Colorectal Cancer Patients Receiving FOLFOX4 Chemotherapy Treatment","volume":"16","author":"Zhou","year":"2023","journal-title":"Int. J. Gen. Med."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Tsokkou, S., Konstantinidis, I., Papakonstantinou, M., Chatzikomnitsa, P., Liampou, E., Toutziari, E., Giakoustidis, D., Bangeas, P., Papadopoulos, V., and Giakoustidis, A. (2025). Sex Differences in Colorectal Cancer: Epidemiology, Risk Factors, and Clinical Outcomes. J. Clin. Med., 14.","DOI":"10.3390\/jcm14155539"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1186\/s12964-024-01549-2","article-title":"Sex Differences in Colorectal Cancer: With a Focus on Sex Hormone-Gut Microbiome Axis","volume":"22","author":"Wu","year":"2024","journal-title":"Cell Commun. Signal."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1186\/1756-9966-28-132","article-title":"MMP7 Expression Regulated by Endocrine Therapy in ERbeta-Positive Colon Cancer Cells","volume":"28","author":"Fang","year":"2009","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"4905","DOI":"10.1038\/s41598-020-61851-0","article-title":"Sex Differences in Colon Cancer Metabolism Reveal A Novel Subphenotype","volume":"10","author":"Cai","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3576","DOI":"10.1038\/s41598-024-53861-z","article-title":"Sex Differences in Colonic Gene Expression and Fecal Microbiota Composition in a Mouse Model of Obesity-Associated Colorectal Cancer","volume":"14","author":"Chang","year":"2024","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"21556","DOI":"10.1038\/s41598-021-01086-9","article-title":"Different Prognostic Impact of Glucose Uptake in Visceral Adipose Tissue According to Sex in Patients With Colorectal Cancer","volume":"11","author":"Lee","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Geddes, A.E., Ray, A.L., Nofchissey, R.A., Esmaeili, A., Saunders, A., Bender, D.E., Khan, M., Aravindan, S., Ahrendsen, J.T., and Li, M. (2022). An Analysis of Sexual Dimorphism in the Tumor Microenvironment of Colorectal Cancer. Front. Oncol., 12.","DOI":"10.3389\/fonc.2022.986103"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1038\/s41416-020-0913-8","article-title":"The Role of Sex in the Innate and Adaptive Immune Environment of Metastatic Colorectal Cancer","volume":"123","author":"Ray","year":"2020","journal-title":"Br. J. Cancer"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"4542","DOI":"10.1200\/JCO.2009.22.0764","article-title":"Use of Hormone Replacement Therapy and the Risk of Colorectal Cancer","volume":"27","author":"Rennert","year":"2009","journal-title":"J. Clin. Oncol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/s13277-010-0080-2","article-title":"MMP-7 as a Prognostic Marker in Colorectal Cancer","volume":"32","author":"Koskensalo","year":"2011","journal-title":"Tumour Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1002\/ijc.20697","article-title":"Matrilysin (MMP-7) Promotes Invasion of Ovarian Cancer Cells by Activation of Progelatinase","volume":"114","author":"Wang","year":"2005","journal-title":"Int. J. Cancer"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Vecurkovska, I., Maslankova, J., Tomeckova, V., Katuchova, J., Kiskova, T., Frohlichova, L., Marekova, M., and Stupak, M. (2023). Stage-Dependent Levels of Brain-Derived Neurotrophic Factor and Matrix Metalloproteinase 9 in the Prognosis of Colorectal Cancer. Biomedicines, 11.","DOI":"10.3390\/biomedicines11071839"}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/2\/214\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T10:42:40Z","timestamp":1767955360000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/2\/214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,9]]},"references-count":47,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,1]]}},"alternative-id":["cancers18020214"],"URL":"https:\/\/doi.org\/10.3390\/cancers18020214","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,9]]}}}