{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T19:28:34Z","timestamp":1769801314727,"version":"3.49.0"},"reference-count":82,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Orchid","award":["C16420"],"award-info":[{"award-number":["C16420"]}]},{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"crossref","award":["A18066"],"award-info":[{"award-number":["A18066"]}],"id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background\/Objectives: Although 90% of prostate cancer (PCa) metastasis occurs in the bone, there are limited studies and rarely available genome-wide profiles at individual sample level for genomic copy number changes in the literature. Methods: We performed Affymetrix SNP 6.0 high-density microarray analysis to generate the genome-wide copy number change profiles for six cases of PCa bone metastases. A common genomic loss was confirmed by fluorescence in situ hybridization (FISH) in paraffin-embedded PCa bone metastasis samples together with primary PCa and benign prostate hyperplasia samples. We overexpressed the candidate miRNA in PCa cell lines and knocked down its target genes by siRNA transfection and investigated the effect on protein expression and cell viability, migration, and invasion abilities, respectively. Protein expression in PCa tissues was analyzed by immunohistochemical staining. Results: We provided high-resolution PCa bone metastasis profiles of six cases and identified potential bone metastasis-specific common genomic alterations, including a 1.6 mb region on 17q11.2, as well as those shared by non-bone metastatic PCa. The common 17q11.2 loss was confirmed by FISH in further 14\/21 PCa bone metastasis samples but was only found in 9\/151 primary PCa samples. The well-established tumor-suppressing miRNA located within this small genomic region, miR-193a-3p, was downregulated in both bone metastasis and primary PCa cases, leading to overexpression of cyclin D1 and uPA to promote cancer cell migration and invasion. Cyclin D1 was highly expressed in both localized PCa and bone metastasis samples, and the expression was significantly higher in the latter group (p = 0.013). Conclusions: We generated high-resolution copy number change profiles for bone metastasis samples. This led to the identification of a common, small genomic loss and downregulation of miR-193a-3p, which suppresses PCa bone metastasis through inhibition of its target proteins, providing new insight into bone metastasis development.<\/jats:p>","DOI":"10.3390\/cancers18030403","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T15:41:56Z","timestamp":1769528516000},"page":"403","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High Frequency Loss of 17q11.2 and Downregulation of the Cancer Metastasis Suppression microRNA miR-193a-3p in Prostate Cancer Bone Metastasis"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0812-4086","authenticated-orcid":false,"given":"Elzbieta","family":"Stankiewicz","sequence":"first","affiliation":[{"name":"Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah C.","family":"McCarley","sequence":"additional","affiliation":[{"name":"Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xueying","family":"Mao","sequence":"additional","affiliation":[{"name":"Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakunthala","family":"Kudahetti","sequence":"additional","affiliation":[{"name":"Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim","family":"Oliver","sequence":"additional","affiliation":[{"name":"Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan","family":"Shamash","sequence":"additional","affiliation":[{"name":"Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trevor","family":"Graham","sequence":"additional","affiliation":[{"name":"Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel M.","family":"Berney","sequence":"additional","affiliation":[{"name":"Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6174-6621","authenticated-orcid":false,"given":"Yong-Jie","family":"Lu","sequence":"additional","affiliation":[{"name":"Barts Cancer Institute, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1088","DOI":"10.1002\/pros.24360","article-title":"Current and projected number of years of life lost due to prostate cancer: A global study","volume":"82","author":"Withrow","year":"2022","journal-title":"Prostate"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.eururo.2024.11.013","article-title":"Recent Patterns and Trends in Global Prostate Cancer Incidence and Mortality: An Update","volume":"87","author":"Schafer","year":"2025","journal-title":"Eur. Urol."},{"key":"ref_3","first-page":"229","article-title":"Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. Clin."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1111\/his.70033","article-title":"Molecular subtypes of metastatic prostate cancer: From pathophysiology to diagnosis","volume":"88","author":"Dsouza","year":"2026","journal-title":"Histopathology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1038\/s41571-021-00499-9","article-title":"Novel approaches to target the microenvironment of bone metastasis","volume":"18","author":"Hofbauer","year":"2021","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1053\/hp.2000.6698","article-title":"Metastatic patterns of prostate cancer: An autopsy study of 1589 patients","volume":"31","author":"Bubendorf","year":"2000","journal-title":"Hum. Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1023\/A:1015599831232","article-title":"Prostate carcinoma skeletal metastases: Cross-talk between tumor and bone","volume":"20","author":"Keller","year":"2001","journal-title":"Cancer Metastasis Rev."},{"key":"ref_8","first-page":"103","article-title":"Bone metastasis in prostate cancer: Molecular and cellular mechanisms","volume":"20","author":"Ye","year":"2007","journal-title":"Int. J. Mol. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1101\/gr.107961.110","article-title":"Copy number and targeted mutational analysis reveals novel somatic events in metastatic prostate tumors","volume":"21","author":"Robbins","year":"2011","journal-title":"Genome Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1038\/nm.1944","article-title":"Copy number analysis indicates monoclonal origin of lethal metastatic prostate cancer","volume":"15","author":"Liu","year":"2009","journal-title":"Nat. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1016\/j.cell.2015.05.001","article-title":"Integrative clinical genomics of advanced prostate cancer","volume":"161","author":"Robinson","year":"2015","journal-title":"Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1200\/PO.18.00283","article-title":"Prospective Comprehensive Genomic Profiling of Primary and Metastatic Prostate Tumors","volume":"3","author":"Chung","year":"2019","journal-title":"JCO Precis. Oncol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"5124","DOI":"10.1038\/s41598-017-05209-z","article-title":"Identification of FBXL4 as a Metastasis Associated Gene in Prostate Cancer","volume":"7","author":"Stankiewicz","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1186\/s13073-025-01445-5","article-title":"Whole genome sequencing of 378 prostate cancer metastases reveals tissue selectivity for mismatch deficiency with potential therapeutic implications","volume":"17","author":"Vis","year":"2025","journal-title":"Genome Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.bone.2015.04.046","article-title":"Bone metastasis in prostate cancer: Recurring mitochondrial DNA mutation reveals selective pressure exerted by the bone microenvironment","volume":"78","author":"Arnold","year":"2015","journal-title":"Bone"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3924","DOI":"10.1158\/1078-0432.CCR-10-3120","article-title":"Characterization of bone metastases from rapid autopsies of prostate cancer patients","volume":"17","author":"Mehra","year":"2011","journal-title":"Clin. Cancer Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3287","DOI":"10.2741\/2312","article-title":"Chromosomal aberrations in prostate cancer","volume":"12","author":"Saramaki","year":"2007","journal-title":"Front. Biosci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"7793","DOI":"10.1158\/0008-5472.CAN-08-3810","article-title":"Comparative analyses of chromosome alterations in soft-tissue metastases within and across patients with castration-resistant prostate cancer","volume":"69","author":"Holcomb","year":"2009","journal-title":"Cancer Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"822","DOI":"10.1038\/35090585","article-title":"Delineation of prognostic biomarkers in prostate cancer","volume":"412","author":"Dhanasekaran","year":"2001","journal-title":"Nature"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"11428","DOI":"10.1073\/pnas.1902651116","article-title":"Genomic correlates of clinical outcome in advanced prostate cancer","volume":"116","author":"Abida","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"5207","DOI":"10.1158\/0008-5472.CAN-09-4074","article-title":"Distinct genomic alterations in prostate cancers in Chinese and Western populations suggest alternative pathways of prostate carcinogenesis","volume":"70","author":"Mao","year":"2010","journal-title":"Cancer Res."},{"key":"ref_22","unstructured":"Cancer Genome Atlas Research Network (2015). The Molecular Taxonomy of Primary Prostate Cancer. Cell, 163, 1011\u20131025."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1518","DOI":"10.1158\/1078-0432.CCR-21-3940","article-title":"Tumor Genomic Testing for >4000 Men with Metastatic Castration-resistant Prostate Cancer in the Phase III Trial PROfound (Olaparib)","volume":"28","author":"Hussain","year":"2022","journal-title":"Clin. Cancer Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"16910","DOI":"10.1073\/pnas.1009843107","article-title":"Allele-specific copy number analysis of tumors","volume":"107","author":"Nordgard","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1111\/j.1442-2042.2006.01399.x","article-title":"Fluorescence in situ hybridization analysis of c-myc amplification in stage TNM prostate cancer in Japanese patients","volume":"13","author":"Sato","year":"2006","journal-title":"Int. J. Urol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"141","DOI":"10.2353\/jmoldx.2006.050083","article-title":"FISH analysis for the detection of lymphoma-associated chromosomal abnormalities in routine paraffin-embedded tissue","volume":"8","author":"Ventura","year":"2006","journal-title":"J. Mol. Diagn."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.cancergen.2013.03.001","article-title":"Analysis of genomic abnormalities in tumors: A review of available methods for Illumina two-color SNP genotyping and evaluation of performance","volume":"206","author":"Oros","year":"2013","journal-title":"Cancer Genet."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1093\/bioinformatics\/btaa538","article-title":"Allele-specific multi-sample copy number segmentation in ASCAT","volume":"37","author":"Ross","year":"2021","journal-title":"Bioinformatics"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5913195","DOI":"10.1155\/2017\/5913195","article-title":"Biological Function of MicroRNA193a-3p in Health and Disease","volume":"2017","author":"Grossi","year":"2017","journal-title":"Int. J. Genomics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"16861","DOI":"10.1002\/jcp.28368","article-title":"miR-193: A new weapon against cancer","volume":"234","author":"Khordadmehr","year":"2019","journal-title":"J. Cell Physiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.2147\/CMAR.S190669","article-title":"Circular RNA circHIPK3 promotes cell proliferation and invasion of prostate cancer by sponging miR-193a-3p and regulating MCL1 expression","volume":"11","author":"Chen","year":"2019","journal-title":"Cancer Manag. Res."},{"key":"ref_32","first-page":"5121","article-title":"MicroRNA-193a-3p inhibits cell proliferation in prostate cancer by targeting cyclin D1","volume":"14","author":"Liu","year":"2017","journal-title":"Oncol. Lett."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1111\/febs.14724","article-title":"PTP1B markedly promotes breast cancer progression and is regulated by miR-193a-3p","volume":"286","author":"Yu","year":"2019","journal-title":"FEBS J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.canlet.2019.01.027","article-title":"miR-193a-3p inhibition of the Slug activator PAK4 suppresses non-small cell lung cancer aggressiveness via the p53\/Slug\/L1CAM pathway","volume":"447","author":"Liu","year":"2019","journal-title":"Cancer Lett."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Polini, B., Carpi, S., Doccini, S., Citi, V., Martelli, A., Feola, S., Santorelli, F.M., Cerullo, V., Romanini, A., and Nieri, P. (2020). Tumor Suppressor Role of hsa-miR-193a-3p and -5p in Cutaneous Melanoma. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176183"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5281","DOI":"10.1158\/1078-0432.CCR-17-0171","article-title":"miR-193a-3p is a Key Tumor Suppressor in Ulcerative Colitis-Associated Colon Cancer and Promotes Carcinogenesis through Upregulation of IL17RD","volume":"23","author":"Pekow","year":"2017","journal-title":"Clin. Cancer Res."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1186\/s12967-025-06689-8","article-title":"miR-193a-3p prevents tumour progression by targeting TCL1 in diffuse large B-cell lymphoma","volume":"23","author":"Gao","year":"2025","journal-title":"J. Transl. Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e2307937","DOI":"10.1002\/advs.202307937","article-title":"Tryptophan As a New Member of RNA-Induced Silencing Complexes Prevents Colon Cancer Liver Metastasis","volume":"11","author":"Xu","year":"2024","journal-title":"Adv. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"e1402","DOI":"10.1038\/cddis.2014.367","article-title":"The DNA methylation-regulated miR-193a-3p dictates the multi-chemoresistance of bladder cancer via repression of SRSF2\/PLAU\/HIC2 expression","volume":"5","author":"Lv","year":"2014","journal-title":"Cell Death Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1007\/s12032-025-02900-4","article-title":"The emerging role of miR-362 in cancer: Expression and function across different cancer types","volume":"42","author":"Younis","year":"2025","journal-title":"Med. Oncol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.3892\/ol.2015.3993","article-title":"Downregulation of miR-362-5p inhibits proliferation, migration and invasion of human breast cancer MCF7 cells","volume":"11","author":"Ni","year":"2016","journal-title":"Oncol. Lett."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1089\/humc.2019.040","article-title":"miR-632 Functions as Oncogene in Hepatocellular Carcinoma via Targeting MYCT1","volume":"30","author":"Pu","year":"2019","journal-title":"Hum. Gene Ther. Clin. Dev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2304","DOI":"10.18632\/aging.203939","article-title":"Downregulation of ZC3H13 by miR-362-3p\/miR-425-5p is associated with a poor prognosis and adverse outcomes in hepatocellular carcinoma","volume":"14","author":"Wu","year":"2022","journal-title":"Aging"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1016\/j.canlet.2014.10.041","article-title":"MicroRNA-362-5p promotes tumor growth and metastasis by targeting CYLD in hepatocellular carcinoma","volume":"356","author":"Ni","year":"2015","journal-title":"Cancer Lett."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/1479-5876-12-33","article-title":"MicroRNA-362 induces cell proliferation and apoptosis resistance in gastric cancer by activation of NF-kappaB signaling","volume":"12","author":"Xia","year":"2014","journal-title":"J. Transl. Med."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Shi, Y., Huang, X., Chen, G., Wang, Y., Liu, Y., Xu, W., Tang, S., Guleng, B., Liu, J., and Ren, J. (2019). miR-632 promotes gastric cancer progression by accelerating angiogenesis in a TFF1-dependent manner. BMC Cancer, 19.","DOI":"10.1186\/s12885-018-5247-z"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Wei, X., Wang, B., Wang, Q., Yang, X., Yang, Y., Fang, Z., Yi, C., Shi, L., Fan, X., and Tao, J. (2020). MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI. Front. Pharmacol., 11.","DOI":"10.3389\/fphar.2020.00164"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3727\/096504018X15213142076069","article-title":"miR-632 Promotes Laryngeal Carcinoma Cell Proliferation, Migration, and Invasion Through Negative Regulation of GSK3beta","volume":"28","author":"Zhou","year":"2020","journal-title":"Oncol. Res."},{"key":"ref_49","first-page":"8931","article-title":"MicroRNA-362 inhibits cell growth and metastasis in glioblastoma by targeting MAPK1","volume":"24","author":"Shi","year":"2020","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1007\/s13577-019-00319-4","article-title":"miR-362-5p promotes cell proliferation and cell cycle progression by targeting GAS7 in acute myeloid leukemia","volume":"33","author":"Wu","year":"2020","journal-title":"Hum. Cell"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s12967-018-1445-3","article-title":"MiR-362-5p as a novel prognostic predictor of cytogenetically normal acute myeloid leukemia","volume":"16","author":"Ma","year":"2018","journal-title":"J. Transl. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.3892\/etm.2020.8857","article-title":"miR-365b regulates the development of non-small cell lung cancer via GALNT4","volume":"20","author":"Xing","year":"2020","journal-title":"Exp. Ther. Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2019","DOI":"10.2217\/fon-2018-0676","article-title":"miRNA-365b promotes hepatocellular carcinoma cell migration and invasion by downregulating SGTB","volume":"15","author":"Tian","year":"2019","journal-title":"Future Oncol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/s12964-024-02001-1","article-title":"Non-coding RNAs secreted by renal cancer include piR_004153 that promotes migration of mesenchymal stromal cells","volume":"23","author":"Boguslawska","year":"2025","journal-title":"Cell Commun. Signal."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1111\/jnc.14459","article-title":"miR-4725-3p targeting stromal interacting molecule 1 signaling is involved in xanthohumol inhibition of glioma cell invasion","volume":"146","author":"Ho","year":"2018","journal-title":"J. Neurochem."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Mlakar, V., Dupanloup, I., Gonzales, F., Papangelopoulou, D., Ansari, M., and Gumy-Pause, F. (2024). 17q Gain in Neuroblastoma: A Review of Clinical and Biological Implications. Cancers, 16.","DOI":"10.3390\/cancers16020338"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1002\/gcc.1165","article-title":"Gain of 1q and loss of 9q21.3-q32 are associated with a less favorable prognosis in papillary thyroid carcinoma","volume":"32","author":"Kjellman","year":"2001","journal-title":"Genes Chromosomes Cancer"},{"key":"ref_58","first-page":"160","article-title":"Cytogenetics of melanoma and nonmelanoma skin cancer","volume":"Volume 810","author":"Carless","year":"2014","journal-title":"Sunlight, Vitamin D and Skin Cancer. Advances in Experimental Medicine and Biology"},{"key":"ref_59","first-page":"897","article-title":"Chromosomal instability and human hepatocarcinogenesis","volume":"18","author":"Nishida","year":"2003","journal-title":"Histol. Histopathol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"530","DOI":"10.1002\/mpo.10180","article-title":"Genetic alterations in hepatoblastoma and hepatocellular carcinoma: Common and distinctive aspects","volume":"39","author":"Buendia","year":"2002","journal-title":"Med. Pediatr. Oncol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"R37","DOI":"10.1530\/JME-12-0069","article-title":"Molecular pathology and genetics of pancreatic endocrine tumours","volume":"49","author":"Capurso","year":"2012","journal-title":"J. Mol. Endocrinol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"4773","DOI":"10.3892\/ol.2016.5271","article-title":"Impact of chromosome 17q deletion in the primary lesion of colorectal cancer on liver metastasis","volume":"12","author":"Kawai","year":"2016","journal-title":"Oncol. Lett."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"e14302","DOI":"10.1097\/MD.0000000000014302","article-title":"Integrative analysis reveals driver long non-coding RNAs in osteosarcoma","volume":"98","author":"Luo","year":"2019","journal-title":"Medicine"},{"key":"ref_64","first-page":"1004","article-title":"Consortium study on 1280 breast carcinomas: Allelic loss on chromosome 17 targets subregions associated with family history and clinical parameters","volume":"58","author":"Phelan","year":"1998","journal-title":"Cancer Res."},{"key":"ref_65","first-page":"879","article-title":"Analysis of colorectal cancer by comparative genomic hybridization: Evidence for induction of the metastatic phenotype by loss of tumor suppressor genes","volume":"4","author":"Kang","year":"1998","journal-title":"Clin. Cancer Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1038\/nrc3932","article-title":"MicroRNA biogenesis pathways in cancer","volume":"15","author":"Lin","year":"2015","journal-title":"Nat. Rev. Cancer"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"28176","DOI":"10.1038\/srep28176","article-title":"Arm Selection Preference of MicroRNA-193a Varies in Breast Cancer","volume":"6","author":"Tsai","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1016\/j.ctrv.2007.06.003","article-title":"Targeting uPA\/uPAR in prostate cancer","volume":"33","author":"Li","year":"2007","journal-title":"Cancer Treat. Rev."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Mahmood, N., Mihalcioiu, C., and Rabbani, S.A. (2018). Multifaceted Role of the Urokinase-Type Plasminogen Activator (uPA) and Its Receptor (uPAR): Diagnostic, Prognostic, and Therapeutic Applications. Front. Oncol., 8.","DOI":"10.3389\/fonc.2018.00024"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1083\/jcb.111.2.783","article-title":"The receptor for urokinase type plasminogen activator polarizes expression of the protease to the leading edge of migrating monocytes and promotes degradation of enzyme inhibitor complexes","volume":"111","author":"Estreicher","year":"1990","journal-title":"J. Cell Biol."},{"key":"ref_71","first-page":"3066","article-title":"Binding of urokinase to its receptor promotes migration and invasion of human melanoma cells in vitro","volume":"54","author":"Stahl","year":"1994","journal-title":"Cancer Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"5021","DOI":"10.1073\/pnas.90.11.5021","article-title":"Prevention of metastasis by inhibition of the urokinase receptor","volume":"90","author":"Crowley","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1593\/neo.10296","article-title":"An anti-urokinase plasminogen activator receptor antibody (ATN-658) blocks prostate cancer invasion, migration, growth, and experimental skeletal metastasis in vitro and in vivo","volume":"12","author":"Rabbani","year":"2010","journal-title":"Neoplasia"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1593\/neo.08106","article-title":"Prostate cancer cell-derived urokinase-type plasminogen activator contributes to intraosseous tumor growth and bone turnover","volume":"10","author":"Dong","year":"2008","journal-title":"Neoplasia"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"4729","DOI":"10.1182\/blood-2008-08-176438","article-title":"Urokinase-mediated recruitment of myeloid-derived suppressor cells and their suppressive mechanisms are blocked by MUC1\/sec","volume":"113","author":"Ilkovitch","year":"2009","journal-title":"Blood"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1200\/JCO.2006.05.6853","article-title":"Association of the circulating levels of the urokinase system of plasminogen activation with the presence of prostate cancer and invasion, progression, and metastasis","volume":"25","author":"Shariat","year":"2007","journal-title":"J. Clin. Oncol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/j.1464-410X.2008.08298.x","article-title":"Urokinase-plasminogen-activator receptor expression in disseminated tumour cells in the bone marrow and peripheral blood of patients with clinically localized prostate cancer","volume":"104","author":"Thomas","year":"2009","journal-title":"BJU Int."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/978-1-4614-0254-1_8","article-title":"Role of epigenetics in cancer initiation and progression","volume":"Volume 720","author":"Chik","year":"2011","journal-title":"Human Cell Transformation. Advances in Experimental Medicine and Biology"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1186\/s13046-020-01723-7","article-title":"Synergistic antitumor interaction of valproic acid and simvastatin sensitizes prostate cancer to docetaxel by targeting CSCs compartment via YAP inhibition","volume":"39","author":"Iannelli","year":"2020","journal-title":"J. Exp. Clin. Cancer Res."},{"key":"ref_80","first-page":"17","article-title":"Osteoblasts modulate secretion of urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP-9) in human prostate cancer cells promoting migration and matrigel invasion","volume":"11","author":"Festuccia","year":"1999","journal-title":"Oncol. Res."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1007\/s10585-009-9289-0","article-title":"Modulation of prostate cancer cell gene expression by cell-to-cell contact with bone marrow stromal cells or osteoblasts","volume":"26","author":"Zhang","year":"2009","journal-title":"Clin. Exp. Metastasis"},{"key":"ref_82","first-page":"2372","article-title":"Urokinase overproduction results in increased skeletal metastasis by prostate cancer cells in vivo","volume":"54","author":"Achbarou","year":"1994","journal-title":"Cancer Res."}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/3\/403\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T05:11:48Z","timestamp":1769749908000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/3\/403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,27]]},"references-count":82,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["cancers18030403"],"URL":"https:\/\/doi.org\/10.3390\/cancers18030403","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,27]]}}}