{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T10:14:26Z","timestamp":1771409666240,"version":"3.50.1"},"reference-count":68,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Commission, Seventh Framework Programme","award":["LSHB-CT-2006-037661"],"award-info":[{"award-number":["LSHB-CT-2006-037661"]}]},{"name":"Studiumstiftung des Deutschen Volkes"},{"name":"Clinical Lipidomics Unit at University Medical Center of Mainz"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background: Altered glycosphingolipidome in cancerous tissues and cells reflects the circulatory glycosphingolipid (GSL) profiles, which is advantageous for establishing cancer biomarkers and\/or unravelling GSL-associated mechanisms of immunity in cancer. Methods: Here, we combined a microscale extraction of GSLs with multiple overlay TLC assays and IR-MALDI-o-TOF MS and implemented it for the first time in serum analysis of CD75s-, CD15s-, and iso-CD75s-containing sialylated GSLs of ganglio- and neolacto-series. Results: This sensitive antigen-specific targeted GSL workflow enabled the identification of 80 sialylated GSLs containing the specific antigens in human sera and was applied for the investigation of clinical serum samples from gastric\/stomach cancer patients (n = 40), pancreatic cancer patients (n = 40), and a cancer-free control group (n = 20). The CD75s-, CD15s-, and iso-CD75s-containing GSL series encompassing complex monosialylated and fucosylated GSLs of neolacto-series, with up to pentadecasaccharide chains, were detected in both cancer types, while differential semi-quantitative analysis indicates a tumor type-specific associated GSL profile. Both cancer types share a drop in the complex fucosylated neolacto-gangliosides during tumor progression, implying a decreased synthesis of long-chain neolacto-series. Conclusions: This drop suggesting a role of these highly polar complex ganglioside species in evading humoral tumor immune response in the early tumor stages.<\/jats:p>","DOI":"10.3390\/cancers18040663","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T09:22:46Z","timestamp":1771406566000},"page":"663","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antigen-Specific Ganglioside Serological Profile of Pancreatic and Gastric Cancer Patients by Multiple TLC Overlay Assay and IR-MALDI Mass Spectrometry"],"prefix":"10.3390","volume":"18","author":[{"given":"Jamal","family":"Souady","sequence":"first","affiliation":[{"name":"QIAGEN GmbH, 40724 Hilden, Germany"},{"name":"Institute of Medical Physics and Biophysics, University of M\u00fcnster, D-48149 M\u00fcnster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephan","family":"Kirsch","sequence":"additional","affiliation":[{"name":"Institute of Medical Physics and Biophysics, University of M\u00fcnster, D-48149 M\u00fcnster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"H\u00fclsewig","sequence":"additional","affiliation":[{"name":"Institute of Medical Physics and Biophysics, University of M\u00fcnster, D-48149 M\u00fcnster, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5220-4334","authenticated-orcid":false,"given":"Romana","family":"Masnikosa","sequence":"additional","affiliation":[{"name":"Department of Physical Chemistry, Institute of Nuclear Sciences Vinca, University of Belgrade, Mike Petrovica Alasa 12-14, 11000 Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2025-6313","authenticated-orcid":false,"given":"Huong Giang","family":"Vo","sequence":"additional","affiliation":[{"name":"Clinical Lipidomics Unit, Institute of Physiological Chemistry, University Medical Center Mainz, D-55128 Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasna","family":"Peter-Katalini\u0107","sequence":"additional","affiliation":[{"name":"Institute of Medical Physics and Biophysics, University of M\u00fcnster, D-48149 M\u00fcnster, Germany"},{"name":"Department of Biotechnology, University of Rijeka, 51000 Rijeka, Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5538-2008","authenticated-orcid":false,"given":"Laura","family":"Bindila","sequence":"additional","affiliation":[{"name":"Clinical Lipidomics Unit, Institute of Physiological Chemistry, University Medical Center Mainz, D-55128 Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1002\/cncr.33587","article-title":"The Ever-increasing Importance of Cancer as a Leading Cause of Premature Death Worldwide","volume":"127","author":"Bray","year":"2021","journal-title":"Cancer"},{"key":"ref_2","first-page":"209","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. Clin."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1136\/gutjnl-2019-320234","article-title":"Is Gastric Cancer Becoming a Rare Disease? A Global Assessment of Predicted Incidence Trends to 2035","volume":"69","author":"Arnold","year":"2020","journal-title":"Gut"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4045","DOI":"10.3748\/wjg.v27.i26.4045","article-title":"Biomarkers in the Diagnosis of Pancreatic Cancer: Are We Closer to Finding the Golden Ticket?","volume":"27","author":"Stoita","year":"2021","journal-title":"World J. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/1751-2980.13070","article-title":"Trends of Stomach Cancer Survival: A Systematic Review of Survival Rates from Population-based Cancer Registration","volume":"23","author":"Tuo","year":"2022","journal-title":"J. Dig. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Huss, R. (2015). Biomarkers. Translational Regenerative Medicine, Elsevier.","DOI":"10.1016\/B978-0-12-410396-2.00019-0"},{"key":"ref_7","unstructured":"Schnaar, R.L., Sandhoff, R., Tiemeyer, M., and Kinoshita, T. (2022). Glycosphingolipids. Essentials of Glycobiology, Cold Spring Harbor Laboratory Press. [4th ed.]."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1021\/acs.jproteome.1c00911","article-title":"Glycosphingolipid-Glycan Signatures of Acute Myeloid Leukemia Cell Lines Reflect Hematopoietic Differentiation","volume":"21","author":"Wang","year":"2022","journal-title":"J. Proteome Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e107238","DOI":"10.15252\/embj.2020107238","article-title":"Golgi Maturation-dependent Glycoenzyme Recycling Controls Glycosphingolipid Biosynthesis and Cell Growth via GOLPH3","volume":"40","author":"Rizzo","year":"2021","journal-title":"EMBO J."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"19369","DOI":"10.1038\/s41598-019-55867-4","article-title":"Ganglioside GM2, Highly Expressed in the MIA PaCa-2 Pancreatic Ductal Adenocarcinoma Cell Line, Is Correlated with Growth, Invasion, and Advanced Stage","volume":"9","author":"Sasaki","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1093\/glycob\/cwv032","article-title":"Expression of Stage-Specific Embryonic Antigen-4 (SSEA-4) Defines Spontaneous Loss of Epithelial Phenotype in Human Solid Tumor Cells","volume":"25","author":"Sivasubramaniyan","year":"2015","journal-title":"Glycobiology"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Sasaki, N., Toyoda, M., and Ishiwata, T. (2021). Gangliosides as Signaling Regulators in Cancer. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22105076"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Zhang, T., de Waard, A.A., Wuhrer, M., and Spaapen, R.M. (2019). The Role of Glycosphingolipids in Immune Cell Functions. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.00090"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.1002\/(SICI)1097-0142(20000415)88:8<1828::AID-CNCR11>3.0.CO;2-F","article-title":"Gangliosides as Targets for Immunotherapy for Pancreatic Adenocarcinoma","volume":"88","author":"Chu","year":"2000","journal-title":"Cancer"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1002\/jcla.1860030508","article-title":"Diagnostic Value of Ganglioside Patterns in Plasma of Human Diseases","volume":"3","author":"Ayabe","year":"1989","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"102923","DOI":"10.1016\/j.jbc.2023.102923","article-title":"Comprehensive Characterization of Complex Glycosphingolipids in Human Pancreatic Cancer Tissues","volume":"299","author":"Jin","year":"2023","journal-title":"J. Biol. Chem."},{"key":"ref_17","first-page":"5","article-title":"Ceramide Profiles of Human Serum Gangliosides GM2 and GD1a Exhibit Cancer-Associated Alterations","volume":"S2","author":"Kirsch","year":"2012","journal-title":"J. Glycom. Lipidom."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2464","DOI":"10.1158\/1535-7163.MCT-08-0353","article-title":"Tumor-Associated CD75s- and Iso-CD75s-Gangliosides Are Potential Targets for Adjuvant Therapy in Pancreatic Cancer","volume":"7","author":"Distler","year":"2008","journal-title":"Mol. Cancer Ther."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1096\/fj.04-2494fje","article-title":"Tumor-associated CD75s Gangliosides and CD75s-bearing Glycoproteins with Neu5Ac\u03b12-6Gal\u03b21-4GlcNAc Residues Are Receptors for the Anticancer Drug RViscumin","volume":"19","author":"Meisen","year":"2005","journal-title":"FASEB J."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1159\/000071205","article-title":"Prognostic Role of Serum Sialyl LewisX (CD15s) in Colorectal Cancer","volume":"65","author":"Paganuzzi","year":"2003","journal-title":"Oncology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1093\/glycob\/cwq200","article-title":"Differences in CD75s- and Iso-CD75s-Ganglioside Content and Altered MRNA Expression of Sialyltransferases ST6GAL1 and ST3GAL6 in Human Hepatocellular Carcinomas and Nontumoral Liver Tissues","volume":"21","author":"Souady","year":"2011","journal-title":"Glycobiology"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"117827","DOI":"10.1016\/j.trac.2024.117827","article-title":"Recent Advances, Challenges, and Future Directions in the Mass Spectrometry Analysis of Glycosphingolipids in Biological Samples","volume":"178","year":"2024","journal-title":"TrAC Trends Anal. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"4567","DOI":"10.1038\/s41467-025-59755-6","article-title":"Extended Coverage of Human Serum Glycosphingolipidome by 4D-RP-LC TIMS-PASEF Unravels Association with Parkinson\u2019s Disease","volume":"16","author":"Vo","year":"2025","journal-title":"Nat. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1093\/glycob\/6.2.147","article-title":"Isolation and Structural Characterization of Fucosylated Gangliosides with Linear Poly-N-Acetyllactosaminyl Chains from Human Granulocytes","volume":"6","author":"Spanbroek","year":"1996","journal-title":"Glycobiology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1515\/BC.2009.066","article-title":"On-Line Nano-HPLC\/ESI QTOF MS Monitoring of A2\u20133 and A2\u20136 Sialylation in Granulocyte Glycosphingolipidome","volume":"390","author":"Kirsch","year":"2009","journal-title":"Biol. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1021\/ac702071x","article-title":"Matching IR-MALDI-o-TOF Mass Spectrometry with the TLC Overlay Binding Assay and Its Clinical Application for Tracing Tumor-Associated Glycosphingolipids in Hepatocellular and Pancreatic Cancer","volume":"80","author":"Distler","year":"2008","journal-title":"Anal. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"9481","DOI":"10.1021\/ac901948h","article-title":"Structural Profiling of Individual Glycosphingolipids in a Single Thin-Layer Chromatogram by Multiple Sequential Immunodetection Matched with Direct IR-MALDI-o-TOF Mass Spectrometry","volume":"81","author":"Souady","year":"2009","journal-title":"Anal. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1006\/excr.1997.3619","article-title":"Shedding of Gangliosides by Human Medulloblastoma Cells","volume":"234","author":"Chang","year":"1997","journal-title":"Exp. Cell Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4663","DOI":"10.1021\/cr990347o","article-title":"Polyglycosylceramides, Poly- N -Acetyllactosamine-Containing Glycosphingolipids: Methods of Analysis, Structure, and Presumable Biological Functions","volume":"100","year":"2000","journal-title":"Chem. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"17248","DOI":"10.1074\/jbc.RA118.004854","article-title":"Helicobacter Pylori\u2013Binding Nonacid Glycosphingolipids in the Human Stomach","volume":"293","author":"Jin","year":"2018","journal-title":"J. Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ab.2007.01.034","article-title":"Novel Fucogangliosides Found in Human Colon Adenocarcinoma Tissues by Means of Glycomic Analysis","volume":"364","author":"Korekane","year":"2007","journal-title":"Anal. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"10511","DOI":"10.1016\/S0021-9258(18)90993-2","article-title":"Novel Fucolipids Accumulating in Human Adenocarcinoma. III. A Hybridoma Antibody (FH6) Defining a Human Cancer-Associated Difucoganglioside (VI3NeuAcV3III3Fuc2nLc6)","volume":"259","author":"Fukushi","year":"1984","journal-title":"J. Biol. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"13942","DOI":"10.1016\/S0021-9258(18)68335-8","article-title":"A Novel Tumor-Associated, Developmentally Regulated Glycolipid Antigen Defined by Monoclonal Antibody ACFH-18","volume":"263","author":"Nudelman","year":"1988","journal-title":"J. Biol. Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1093\/glycob\/cwq108","article-title":"Novel Ganglioside Found in Adenocarcinoma Cells of Lewis-Negative Patients","volume":"20","author":"Shida","year":"2010","journal-title":"Glycobiology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"100239","DOI":"10.1016\/j.mcpro.2022.100239","article-title":"High Diversity of Glycosphingolipid Glycans of Colorectal Cancer Cell Lines Reflects the Cellular Differentiation Phenotype","volume":"21","author":"Wang","year":"2022","journal-title":"Mol. Cell. Proteom."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1007\/s10719-019-09885-z","article-title":"Gangliosides Profiling in Serum of Breast Cancer Patient: GM3 as a Potential Diagnostic Biomarker","volume":"36","author":"Li","year":"2019","journal-title":"Glycoconj. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/bs.pmbts.2017.10.003","article-title":"Gangliosides in Cancer Cell Signaling","volume":"156","author":"Dewald","year":"2018","journal-title":"Prog. Mol. Biol. Transl. Sci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1093\/glycob\/cww108","article-title":"Circulating Blood and Platelets Supply Glycosyltransferases That Enable Extrinsic Extracellular Glycosylation","volume":"27","author":"Ashline","year":"2017","journal-title":"Glycobiology"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0304-3835(01)00705-4","article-title":"Increased Expression of Sialyl Lewisx Antigen as a Prognostic Factor in Patients with Stage 0, I, and II Gastric Cancer","volume":"175","author":"Nakagoe","year":"2002","journal-title":"Cancer Lett."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1159\/000048767","article-title":"Overexpression of Sialyl Lewis x Antigen Is Associated with Formation of Extratumoral Venous Invasion and Predicts Postoperative Development of Massive Hepatic Metastasis in Cases with Pancreatic Ductal Adenocarcinoma","volume":"69","author":"Takahashi","year":"2001","journal-title":"Pathobiology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.cdp.2009.01.001","article-title":"High Expression of \u03b1 2, 3-Linked Sialic Acid Residues Is Associated with the Metastatic Potential of Human Gastric Cancer","volume":"32","author":"Wang","year":"2009","journal-title":"Cancer Detect. Prev."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1007\/s12032-010-9771-1","article-title":"Altered MRNA Expressions of Sialyltransferases in Human Gastric Cancer Tissues","volume":"29","author":"Jun","year":"2012","journal-title":"Med. Oncol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1016\/j.biocel.2013.05.015","article-title":"A2,3-Sialyltransferase ST3Gal IV Promotes Migration and Metastasis in Pancreatic Adenocarcinoma Cells and Tends to Be Highly Expressed in Pancreatic Adenocarcinoma Tissues","volume":"45","author":"Arteta","year":"2013","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0005-2760(98)00096-4","article-title":"Natural Forms of Shed Tumor Gangliosides","volume":"1394","author":"Kong","year":"1998","journal-title":"Biochim. Biophys. Acta (BBA)-Lipids Lipid Metab."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1016\/j.bbagen.2005.11.014","article-title":"Shedding and Uptake of Gangliosides and Glycosylphosphatidylinositol-Anchored Proteins","volume":"1760","author":"Lauc","year":"2006","journal-title":"Biochim. Biophys. Acta (BBA)-Gen. Subj."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"912","DOI":"10.1093\/jnci\/92.11.912","article-title":"Influence of Cellular Ganglioside Depletion on Tumor Formation","volume":"92","author":"Deng","year":"2000","journal-title":"J. Natl. Cancer Inst."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Kim, C.-H. (2020). Membrane Ganglioside-Specific Neuraminidase 3 (NEU3) Regulates GM3 Signaling. GM3 Signaling, Springer.","DOI":"10.1007\/978-981-15-5652-4_11"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"532","DOI":"10.1016\/j.bbagen.2007.09.016","article-title":"Roles of Plasma Membrane-Associated Sialidase NEU3 in Human Cancers","volume":"1780","author":"Miyagi","year":"2008","journal-title":"Biochim. Biophys. Acta (BBA)-Gen. Subj."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3330","DOI":"10.4081\/ejh.2022.3330","article-title":"Alpha2,3 Sialic Acid Processing Enzymes Expression in Gastric Cancer Tissues Reveals That ST3Gal3 but Not Neu3 Are Associated with Lauren\u2019s Classification, Angiolymphatic Invasion and Histological Grade","volume":"66","author":"Quirino","year":"2022","journal-title":"Eur. J. Histochem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1038\/s41423-020-0388-9","article-title":"Physiology of Gangliosides and the Role of Antiganglioside Antibodies in Human Diseases","volume":"17","author":"Cutillo","year":"2020","journal-title":"Cell. Mol. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1089\/hyb.1998.17.431","article-title":"Autoantibodies in the Serum of Patients with Gastric Cancer: Their Prognostic Importance","volume":"17","author":"Konstandoulakis","year":"1998","journal-title":"Hybridoma"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2399","DOI":"10.1194\/jlr.D062083","article-title":"Quantitative GSL-Glycome Analysis of Human Whole Serum Based on an EGCase Digestion and Glycoblotting Method","volume":"56","author":"Furukawa","year":"2015","journal-title":"J. Lipid Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1002\/ijc.29034","article-title":"Variation at ABO Histo-blood Group and FUT Loci and Diffuse and Intestinal Gastric Cancer Risk in a European Population","volume":"136","author":"Duell","year":"2015","journal-title":"Int. J. Cancer"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1016\/S0300-9084(01)01301-3","article-title":"The Human Sialyltransferase Family","volume":"83","author":"Julien","year":"2001","journal-title":"Biochimie"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Groux-Degroote, S., and Delannoy, P. (2021). Cancer-Associated Glycosphingolipids as Tumor Markers and Targets for Cancer Immunotherapy. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22116145"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Blanas, A., Sahasrabudhe, N.M., Rodr\u00edguez, E., van Kooyk, Y., and van Vliet, S.J. (2018). Fucosylated Antigens in Cancer: An Alliance toward Tumor Progression, Metastasis, and Resistance to Chemotherapy. Front. Oncol., 8.","DOI":"10.3389\/fonc.2018.00039"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Trinchera, M., Aronica, A., and Dall\u2019Olio, F. (2017). Selectin Ligands Sialyl-Lewis a and Sialyl-Lewis x in Gastrointestinal Cancers. Biology, 6.","DOI":"10.3390\/biology6010016"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0304-4165(99)00080-X","article-title":"Altered MRNA Expression of Glycosyltransferases in Human Gastric Carcinomas","volume":"1428","author":"Petretti","year":"1999","journal-title":"Biochim. Biophys. Acta (BBA)-Gen. Subj."},{"key":"ref_59","first-page":"39","article-title":"Blood Group ABH and Ii Antigens of Human Erythrocytes: Chemistry, Polymorphism, and Their Developmental Change","volume":"18","author":"Hakomori","year":"1981","journal-title":"Semin. Hematol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/0145-2126(90)90040-G","article-title":"Distribution of VIM-2 and SSEA-1 Glycoconjugate Epitopes among Human Leukocytes and Leukemia Cells","volume":"14","author":"Macher","year":"1990","journal-title":"Leuk. Res."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1093\/oxfordjournals.jbchem.a021263","article-title":"The CDw65 Monoclonal Antibodies VIM-8 and VIM-11 Bind to the Neutral Glycolipid V3FucnLc8Cer","volume":"119","author":"Kniep","year":"1996","journal-title":"J. Biochem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1046\/j.1537-2995.2001.41040504.x","article-title":"Human Platelets Express Gangliosides with LKEactivity and ABH Blood Group Activity","volume":"41","author":"Cooling","year":"2001","journal-title":"Transfusion"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.1182\/blood-2008-04-149641","article-title":"E-Selectin Receptors on Human Leukocytes","volume":"112","author":"Nimrichter","year":"2008","journal-title":"Blood"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1093\/glycob\/9.5.459","article-title":"Isolation and Structural Characterization of Glycosphingolipids of in Vitro Propagated Human Umbilical Vein Endothelial Cells","volume":"9","author":"Muthing","year":"1999","journal-title":"Glycobiology"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Gomes, C., Os\u00f3rio, H., Pinto, M.T., Campos, D., Oliveira, M.J., and Reis, C.A. (2013). Expression of ST3GAL4 Leads to SLex Expression and Induces C-Met Activation and an Invasive Phenotype in Gastric Carcinoma Cells. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0066737"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.3892\/ijo.2017.3882","article-title":"Enhanced Expression of A2,3-Linked Sialic Acids Promotes Gastric Cancer Cell Metastasis and Correlates with Poor Prognosis","volume":"50","author":"Shen","year":"2017","journal-title":"Int. J. Oncol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"22782","DOI":"10.1016\/S0021-9258(18)41595-5","article-title":"Expression Cloning of a Novel Gal Beta (1-3\/1-4) GlcNAc Alpha 2,3-Sialyltransferase Using Lectin Resistance Selection","volume":"268","author":"Sasaki","year":"1993","journal-title":"J. Biol. Chem."},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Laberge, G.S., Duvall, E., Haedicke, K., and Pawelek, J. (2019). Leukocyte\u2013Cancer Cell Fusion\u2014Genesis of a Deadly Journey. Cells, 8.","DOI":"10.3390\/cells8020170"}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/4\/663\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T09:26:52Z","timestamp":1771406812000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/4\/663"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,18]]},"references-count":68,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["cancers18040663"],"URL":"https:\/\/doi.org\/10.3390\/cancers18040663","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,18]]}}}