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Current immunophenotyping panels for diagnosis and detection of measurable residual disease (MRD) primarily focus on detecting blasts, thereby overlooking AML heterogeneity, which could provide valuable prognostic insights. In this context, we propose repositioning EuroFlow panels within a new set of analyses to assess leukemia stem-like phenotypes for AML prognostic monitoring. Methods: We performed a retrospective study with AML patients at the Hospital de Cl\u00ednicas de Porto Alegre between 2015 and 2023. Results: Our findings highlight the relevance of leukemia stem cell-like phenotypes, particularly the CD123+, CD34+CD123+, and CD34+CD38\u2212 subpopulations, for complementary prognostication of adult and elderly patients. A higher percentage of CD123+ cells was a risk factor for both RFS (p = 0.04) and OS (p = 0.02), whereas an increase in CD34+CD123+ cells was a risk factor for RFS (p = 0.04) only. A higher percentage of CD34+CD38\u2212 cells was a risk factor for OS (p = 0.002). From diagnosis to the second monitoring, the CD34+ subpopulation was a more relevant prognostic factor in elderly individuals than in adults. CD34&amp;CD38 most immature subpopulations may increase with poor-prognosis markers in both adults and the elderly. Conclusions: Repositioning immunophenotypic analyses may offer a cost-effective alternative for refined prognostication, particularly in healthcare centers that already have flow cytometry-based AML diagnostics.<\/jats:p>","DOI":"10.3390\/cancers18091394","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:33:37Z","timestamp":1777376017000},"page":"1394","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["LSC-like Phenotypes Aid in the Prognosis of Adult and Elderly Acute Myeloid Leukemia Patients at a Resource-Limited Health Center"],"prefix":"10.3390","volume":"18","author":[{"given":"Camila Kehl","family":"Dias","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas: Bioqu\u00edmica, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3733-3206","authenticated-orcid":false,"given":"Humberto Cardoso","family":"Alves","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8648-9295","authenticated-orcid":false,"given":"Rafaela Bergman","family":"Rostirola","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1958-235X","authenticated-orcid":false,"given":"Maria Fernanda","family":"Gon\u00e7alves Meirelles Fernandes","sequence":"additional","affiliation":[{"name":"Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexia Nedel","family":"Sant\u2019Ana","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas: Bioqu\u00edmica, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8444-4359","authenticated-orcid":false,"given":"Ana Paula","family":"Alegretti","sequence":"additional","affiliation":[{"name":"Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariela Granero","family":"Farias","sequence":"additional","affiliation":[{"name":"Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela Portela","family":"da Silva","sequence":"additional","affiliation":[{"name":"Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3690-3987","authenticated-orcid":false,"given":"Cl\u00e1udia Caceres","family":"Astigarraga","sequence":"additional","affiliation":[{"name":"Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Cl\u00ednica M\u00e9dica, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-903, Brazil"},{"name":"Departamento de Medicina Interna, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9495-6932","authenticated-orcid":false,"given":"Liane Esteves","family":"Daudt","sequence":"additional","affiliation":[{"name":"Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Sa\u00fade da Crian\u00e7a e do Adolescente, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-903, Brazil"},{"name":"Departamento de Pediatria, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9482-0776","authenticated-orcid":false,"given":"Mariana Bohns","family":"Michalowski","sequence":"additional","affiliation":[{"name":"Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, Brazil"},{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Sa\u00fade da Crian\u00e7a e do Adolescente, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-903, Brazil"},{"name":"Departamento de Pediatria, Faculdade de Medicina (FAMED), Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra Aparecida","family":"Paz","sequence":"additional","affiliation":[{"name":"Hospital de Cl\u00ednicas de Porto Alegre, Porto Alegre 90035-903, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0899-6407","authenticated-orcid":false,"given":"Fabr\u00edcio","family":"Figueir\u00f3","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Ci\u00eancias Biol\u00f3gicas: Bioqu\u00edmica, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, Brazil"},{"name":"Departamento de Bioqu\u00edmica, Instituto de Ci\u00eancias B\u00e1sicas da Sa\u00fade, Universidade Federal do Rio Grande do Sul, Porto Alegre 90035-003, 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