{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:55:28Z","timestamp":1792205728392,"version":"4.4.0"},"reference-count":58,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007893","name":"Indiana State University","doi-asserted-by":"crossref","award":["R25MD011712"],"award-info":[{"award-number":["R25MD011712"]}],"id":[{"id":"10.13039\/100007893","id-type":"DOI","asserted-by":"crossref"}]},{"award":["R25MD011712"],"award-info":[{"award-number":["R25MD011712"]}],"id":[{"id":"https:\/\/ror.org\/00f8man71","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background: Digestive tract cancers, like most other cancers, are usually categorized based on cell or tissue of origin. Molecular clustering based on the transcriptome often produces the same classification. Methods: We developed a new method, Newmanization, to reduce underlying tissue signals from transcriptomic analysis. To test our method, we downloaded data on 1635 samples of digestive tract cancers from The Cancer Genome Atlas. The available data includes transcriptomic data by RNA-Seq, as well as binary mutation allele frequency data by whole exome sequencing. We compared, using silhouette widths and visualization by dimension reduction plots, the effectiveness of Newmanized transcriptome and mutation data to separate digestive tract cancers. Results: The Newmanized transcriptome clusters have clearer separation and larger average silhouette widths. Feature analysis of each cluster for Newmanized transcriptomic data and mutation data revealed that clusters determined with Newmanized data contained more mRNAs present at higher frequencies than clusters defined by mutation data. Conclusions: This suggests that the Newmanized method holds great potential for advancing personalized transcriptomic medicine.<\/jats:p>","DOI":"10.3390\/cancers18091427","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T08:46:26Z","timestamp":1777538786000},"page":"1427","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clustering Digestive Tract Tumors Using Transcriptomic and Mutation Data"],"prefix":"10.3390","volume":"18","author":[{"given":"Dwayne G.","family":"Tally","sequence":"first","affiliation":[{"name":"Department of Informatics, Indiana University, Bloomington, IN 47408, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polina","family":"Bombina","sequence":"additional","affiliation":[{"name":"Department of Biostatistics Data Science, Epidemiology Georgia Cancer Center, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jake","family":"Reed","sequence":"additional","affiliation":[{"name":"Department of Oncological Sciences, Huntsman Cancer Institute, Academic Medical Center, Health University of Utah, Salt Lake City, UT 85112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6668-0796","authenticated-orcid":false,"given":"Jeffrey","family":"Kinne","sequence":"additional","affiliation":[{"name":"Department of Computer Science, Indiana State University, Terre Haute, IN 47809, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynne V.","family":"Abruzzo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Medical University of South Carolina, Charleston, SC 29425, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7630-2123","authenticated-orcid":false,"given":"Kevin R.","family":"Coombes","sequence":"additional","affiliation":[{"name":"Department of Biostatistics Data Science, Epidemiology Georgia Cancer Center, Augusta University, Augusta, GA 30912, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary B.","family":"Abrams","sequence":"additional","affiliation":[{"name":"Institute for Informatics, Data Science & Biostatistics, Washington University, St. Louis, MO 63110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"14863","DOI":"10.1073\/pnas.95.25.14863","article-title":"Cluster Analysis and Display of Genome-Wide Expression Patterns","volume":"95","author":"Eisen","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1038\/nature10166","article-title":"Integrated Genomic Analyses of Ovarian Carcinoma","volume":"474","author":"Bell","year":"2011","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1038\/nature11252","article-title":"Comprehensive Molecular Characterization of Human Colon and Rectal Cancer","volume":"487","author":"Muzny","year":"2012","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1038\/nature11412","article-title":"Comprehensive Molecular Portraits of Human Breast Tumours","volume":"490","author":"Koboldt","year":"2012","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1038\/nature11404","article-title":"Comprehensive Genomic Characterization of Squamous Cell Lung Cancers","volume":"489","author":"Hammerman","year":"2012","journal-title":"Nature"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1038\/nature12222","article-title":"Comprehensive Molecular Characterization of Clear Cell Renal Cell Carcinoma","volume":"499","author":"Creighton","year":"2013","journal-title":"Nature"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.cell.2013.09.034","article-title":"The Somatic Genomic Landscape of Glioblastoma","volume":"155","author":"Brennan","year":"2013","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.1038\/ng.2764","article-title":"The Cancer Genome Atlas Pan-Cancer Analysis Project","volume":"45","author":"Weinstein","year":"2013","journal-title":"Nat. Genet."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.cell.2018.03.022","article-title":"Cell-of-Origin Patterns Dominate the Molecular Classification of 10,000 Tumors from 33 Types of Cancer","volume":"173","author":"Hoadley","year":"2018","journal-title":"Cell"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Wu, D., Wang, D., Zhang, M.Q., and Gu, J. (2015). Fast Dimension Reduction and Integrative Clustering of Multi-Omics Data Using Low-Rank Approximation: Application to Cancer Molecular Classification. BMC Genom., 16.","DOI":"10.1186\/s12864-015-2223-8"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"24949","DOI":"10.1038\/srep24949","article-title":"Pan-Cancer Subtyping in a 2D-map Shows Substructures That Are Driven by Specific Combinations of Molecular Characteristics","volume":"6","author":"Taskesen","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s13072-015-0007-7","article-title":"Pan-Cancer Stratification of Solid Human Epithelial Tumors and Cancer Cell Lines Reveals Commonalities and Tissue-Specific Features of the CpG Island Methylator Phenotype","volume":"8","author":"Gotea","year":"2015","journal-title":"Epigenet. Chromatin"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/s41586-020-1943-3","article-title":"The Repertoire of Mutational Signatures in Human Cancer","volume":"578","author":"Alexandrov","year":"2020","journal-title":"Nature"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"104895","DOI":"10.1016\/j.jprot.2023.104895","article-title":"Using Feature Selection and Bayesian Network Identify Cancer Subtypes Based on Proteomic Data","volume":"280","author":"Wang","year":"2023","journal-title":"J. Proteom."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Abrams, Z.B., Zucker, M., Wang, M., Asiaee Taheri, A., Abruzzo, L.V., and Coombes, K.R. (2018). Thirty Biologically Interpretable Clusters of Transcription Factors Distinguish Cancer Type. BMC Genom., 19.","DOI":"10.1186\/s12864-018-5093-z"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1089\/cmb.2019.0321","article-title":"Explaining Gene Expression Using Twenty-One MicroRNAs","volume":"27","author":"Asiaee","year":"2020","journal-title":"J. Comput. Biol. A J. Comput. Mol. Cell Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1093\/jjco\/hyg026","article-title":"Genetic Polymorphisms of Alcohol and Aldehyde Dehydrogenases and Risk for Esophageal and Head and Neck Cancers","volume":"33","author":"Yokoyama","year":"2003","journal-title":"Jpn. J. Clin. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3887","DOI":"10.1038\/ncomms4887","article-title":"A Pan-Cancer Proteomic Perspective on The Cancer Genome Atlas","volume":"5","author":"Akbani","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_19","first-page":"251","article-title":"Gastric Cancer: Classification, Histology and Application of Molecular Pathology","volume":"3","author":"Hu","year":"2012","journal-title":"J. Gastrointest. Oncol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2780","DOI":"10.1093\/bioinformatics\/btab037","article-title":"Mercator: A Pipeline For Multi-Method, Unsupervised Visualization And Distance Generation","volume":"37","author":"Abrams","year":"2021","journal-title":"Bioinformatics"},{"key":"ref_21","first-page":"1","article-title":"Polychrome: Creating and Assessing Qualitative Palettes with Many Colors","volume":"90","author":"Coombes","year":"2019","journal-title":"J. Stat. Softw. Code Snippets"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"13231","DOI":"10.1002\/ece3.5774","article-title":"Silhouette Width Using Generalized Mean-A Flexible Method for Assessing Clustering Efficiency","volume":"9","author":"Lengyel","year":"2019","journal-title":"Ecol. Evol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0377-0427(87)90125-7","article-title":"Silhouettes: A Graphical Aid to the Interpretation and Validation of Cluster Analysis","volume":"20","author":"Rousseeuw","year":"1987","journal-title":"J. Comput. Appl. Math."},{"key":"ref_24","first-page":"2579","article-title":"Visualizing Data Using t-SNE","volume":"9","author":"Hinton","year":"2008","journal-title":"J. Mach. Learn. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1002\/wics.1212","article-title":"The Comprehensive R Archive Network","volume":"4","author":"Hornik","year":"2012","journal-title":"WIREs Comput. Stat."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1093\/biomet\/31.1-2.20","article-title":"The Distribution of Range in Samples from a Normal Population, Expressed in Terms of an Independent Estimate of Standard Deviation","volume":"31","author":"Newman","year":"1939","journal-title":"Biometrika"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1093\/bioinformatics\/17.6.509","article-title":"A Bayesian Framework for the Analysis of Microarray Expression Data: Regularized t -Test and Statistical Inferences of Gene Changes","volume":"17","author":"Baldi","year":"2001","journal-title":"Bioinformatics"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S1046-2023(03)00155-5","article-title":"Normalization of cDNA Microarray Data","volume":"31","author":"Smyth","year":"2003","journal-title":"Methods"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1007\/s10661-018-6461-5","article-title":"Optimizing the Flow Adjustment of Constituent Concentrations via LOESS for Trend Analysis","volume":"190","author":"Simpson","year":"2018","journal-title":"Environ. Monit. Assess."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1111\/j.1469-8137.1912.tb05611.x","article-title":"The distribution of the flora in the alpine zone.1","volume":"11","author":"Jaccard","year":"1912","journal-title":"New Phytol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"W305","DOI":"10.1093\/nar\/gkp427","article-title":"ToppGene Suite for Gene List Enrichment Analysis and Candidate Gene Prioritization","volume":"37","author":"Chen","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e00151","DOI":"10.14309\/ctg.0000000000000151","article-title":"Family History of Colorectal or Esophageal Cancer in Barrett\u2019s Esophagus and Potentially Explanatory Genetic Variants","volume":"11","author":"Rubenstein","year":"2020","journal-title":"Clin. Transl. Gastroenterol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"100653","DOI":"10.1016\/j.jbior.2019.100653","article-title":"NSAID Therapy for PIK3CA-Altered Colorectal, Breast, and Head and Neck Cancer","volume":"75","author":"Cai","year":"2020","journal-title":"Adv. Biol. Regul."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1136\/jmedgenet-2011-100714","article-title":"Correlation of Tumour BRAF Mutations and MLH1 Methylation with Germline Mismatch Repair (MMR) Gene Mutation Status: A Literature Review Assessing Utility of Tumour Features for MMR Variant Classification","volume":"49","author":"Parsons","year":"2012","journal-title":"J. Med. Genet."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2015","DOI":"10.3892\/ijo.2013.2131","article-title":"Functional Impact of Colorectal Cancer-Associated Mutations in the Transcription Factor E2F4","volume":"43","author":"Paquin","year":"2013","journal-title":"Int. J. Oncol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1038\/s41379-019-0289-6","article-title":"Colorectal Carcinoma with Double Somatic Mismatch Repair Gene Inactivation: Clinical and Pathological Characteristics and Response to Immune Checkpoint Blockade","volume":"32","author":"Wang","year":"2019","journal-title":"Mod. Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"224","DOI":"10.4143\/crt.2009.41.4.224","article-title":"Mutational Analysis of TTK Gene in Gastric and Colorectal Cancers with Microsatellite Instability","volume":"41","author":"Ahn","year":"2009","journal-title":"Cancer Res. Treat."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1414","DOI":"10.1038\/modpathol.3800672","article-title":"Immunohistochemical Staining of hMLH1 and hMSH2 Reflects Microsatellite Instability Status in Ovarian Carcinoma","volume":"19","author":"Rosen","year":"2006","journal-title":"Mod. Pathol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1038\/359235a0","article-title":"APC Mutations Occur Early During Colorectal Tumorigenesis","volume":"359","author":"Powell","year":"1992","journal-title":"Nature"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"331","DOI":"10.2174\/156652412799218868","article-title":"Genomic Sequencing of Key Genes in Mouse Pancreatic Cancer Cells","volume":"12","author":"Wang","year":"2012","journal-title":"Curr. Mol. Med."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Hsieh, Y.-Y., Liu, T.-P., Chou, C.-J., Chen, H.-Y., Lee, K.-H., and Yang, P.-M. (2019). Integration of Bioinformatics Resources Reveals the Therapeutic Benefits of Gemcitabine and Cell Cycle Intervention in SMAD4-Deleted Pancreatic Ductal Adenocarcinoma. Genes, 10.","DOI":"10.3390\/genes10100766"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"8154","DOI":"10.1038\/s41598-021-87486-3","article-title":"Enhancing the Landscape of Colorectal Cancer Using Targeted Deep Sequencing","volume":"11","author":"Lee","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1158\/0008-5472.CAN-12-2706","article-title":"SMAD2, SMAD3 and SMAD4 Mutations in Colorectal Cancer","volume":"73","author":"Fleming","year":"2013","journal-title":"Cancer Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/s41418-018-0246-9","article-title":"Mutant P53 as a Guardian of the Cancer Cell","volume":"26","author":"Mantovani","year":"2019","journal-title":"Cell Death Differ."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/nature22341","article-title":"Exosomes Facilitate Therapeutic Targeting of Oncogenic KRAS in Pancreatic Cancer","volume":"546","author":"Kamerkar","year":"2017","journal-title":"Nature"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1038\/s41467-021-22125-z","article-title":"The Origins and Genetic Interactions of KRAS Mutations Are Allele- and Tissue-Specific","volume":"12","author":"Cook","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1007\/s10689-010-9329-6","article-title":"Prevalence of BRCA2 and CDKN2a Mutations in German Familial Pancreatic Cancer Families","volume":"9","author":"Slater","year":"2010","journal-title":"Fam. Cancer"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1158\/1078-0432.CCR-21-4316","article-title":"Association of TP53 and CDKN2A Mutation Profile with Tumor Mutation Burden in Head and Neck Cancer","volume":"28","author":"Deneka","year":"2022","journal-title":"Clin. Cancer Res."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Paschke, S., Jafarov, S., Staib, L., Kreuser, E.-D., Maulbecker-Armstrong, C., Roitman, M., Holm, T., Harris, C.C., Link, K.-H., and Kornmann, M. (2018). Are Colon and Rectal Cancer Two Different Tumor Entities? A Proposal to Abandon the Term Colorectal Cancer. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19092577"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2777","DOI":"10.3892\/ol.2017.5750","article-title":"Identification of Molecular Characteristics Induced by Radiotherapy in Rectal Cancer Based on Microarray Data","volume":"13","author":"Ge","year":"2017","journal-title":"Oncol. Lett."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Hu, J., Guo, J., Pei, Y., Hu, P., Li, M., Sack, I., and Li, W. (2021). Rectal Tumor Stiffness Quantified by In Vivo Tomoelastography and Collagen Content Estimated by Histopathology Predict Tumor Aggressiveness. Front. Oncol., 11.","DOI":"10.3389\/fonc.2021.701336"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"707","DOI":"10.3892\/ijo_00000720","article-title":"Characterization of Rectal, Proximal and Distal Colon Cancers Based on Clinicopathological, Molecular and Protein Profiles","volume":"37","author":"Minoo","year":"2010","journal-title":"Int. J. Oncol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"7303","DOI":"10.1158\/1078-0432.CCR-11-1570","article-title":"Gene Expression Differences Between Colon and Rectum Tumors","volume":"17","author":"Cordero","year":"2011","journal-title":"Clin. Cancer Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1007\/s10388-021-00835-z","article-title":"Whole Exome Sequencing and Deep Sequencing of Esophageal Squamous Cell Carcinoma and Adenocarcinoma in Japanese Patients Using the Japanese Version of the Genome Atlas, JCGA","volume":"18","author":"Booka","year":"2021","journal-title":"Esophagus"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"zcac023","DOI":"10.1093\/narcan\/zcac023","article-title":"KMT2C-deficient Tumors Have Elevated APOBEC Mutagenesis and Genomic Instability in Multiple Cancers","volume":"4","author":"Hu","year":"2022","journal-title":"NAR Cancer"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e14454","DOI":"10.1097\/MD.0000000000014454","article-title":"High Expression of HLA-DQA1 Predicts Poor Outcome in Patients with Esophageal Squamous Cell Carcinoma in Northern China","volume":"98","author":"Shen","year":"2019","journal-title":"Medicine"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1016\/j.ajpath.2010.10.027","article-title":"Antigen Presentation and MHC Class II Expression by Human Esophageal Epithelial Cells: Role in Eosinophilic Esophagitis","volume":"178","author":"Mulder","year":"2011","journal-title":"Am. J. Pathol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1038\/modpathol.2008.172","article-title":"Prognostic Significance of Global Histone Modifications in Resected Squamous Cell Carcinoma of the Esophagus","volume":"22","author":"Tzao","year":"2009","journal-title":"Mod. Pathol."}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/9\/1427\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T04:17:23Z","timestamp":1777695443000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/9\/1427"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,30]]},"references-count":58,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["cancers18091427"],"URL":"https:\/\/doi.org\/10.3390\/cancers18091427","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.01.13.632722","asserted-by":"object"}]},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,30]]}}}