{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T05:21:03Z","timestamp":1788153663619,"version":"build-2803163510"},"reference-count":30,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tunisian ministry of higher education and scientific research","award":["LR19CBS02"],"award-info":[{"award-number":["LR19CBS02"]}]},{"award":["LR19CBS02"],"award-info":[{"award-number":["LR19CBS02"]}],"id":[{"id":"https:\/\/ror.org\/05v1m8q81","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background\/Objectives: This study aims to investigate the mutational spectrum of BRCA1 and BRCA2 genes in a cohort of breast cancer (BC) patients from southern Tunisia, and to evaluate their clinical and prognostic significance. Additionally, this study explores the contribution of other cancer predisposition genes and the prevalence of variants of uncertain significance (VUS). Results: Among the 165 patients included, pathogenic or likely pathogenic variants (P\/LPVs) in BRCA1\/BRCA2 were identified in 19 cases (11.51%), including 8 in BRCA1 and 11 in BRCA2. The presence of BRCA P\/LPVs associated with young patients (p = 0.006) and those with TNBC (p = 0.036). Beyond BRCA1\/2, PV\/LPVs were detected in other cancer-related genes, including TP53 (n = 3), CHEK2, RAD50 (n = 2 cases each), and MUTYH, BARD1, and BRIP1 (one case each). Furthermore, 56 VUS were identified; among them, 7 were prioritized based on in silico predictive analyses, suggesting a potential deleterious effect. However, these VUS should not be used for clinical decision-making without additional evidence from functional and familial segregation studies. Conclusions: Our findings provide novel insights into the genetic landscape of breast cancer in southern Tunisia, highlighting the clinical relevance of BRCA1\/2 mutations and the contribution of other susceptibility genes. These results support the personalized management of breast cancer patients and the implementation of expanded multigene panel testing in routine clinical practice to improve genetic counseling.<\/jats:p>","DOI":"10.3390\/cancers18172810","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T04:24:03Z","timestamp":1788150243000},"page":"2810","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive Genomic Profiling Reveals the Mutational Spectrum and Clinical Significance of BRCA1\/2 and Other Cancer-Susceptibility Genes in Breast Cancer Patients from Southern Tunisia"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5744-0328","authenticated-orcid":false,"given":"Nihel","family":"Ammous-Boukhris","sequence":"first","affiliation":[{"name":"Center of Biotechnology of Sfax, Laboratory of Molecular Biotechnology of Eukaryotes, University of Sfax, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9777-7222","authenticated-orcid":false,"given":"Rania","family":"Abdelmaksoud-Dammak","sequence":"additional","affiliation":[{"name":"Center of Biotechnology of Sfax, Laboratory of Molecular Biotechnology of Eukaryotes, University of Sfax, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4956-9551","authenticated-orcid":false,"given":"Wala","family":"Ben Kridis","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Habib Bourguiba Hospital, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorra","family":"Ben-Ayed-Guerfali","sequence":"additional","affiliation":[{"name":"Center of Biotechnology of Sfax, Laboratory of Molecular Biotechnology of Eukaryotes, University of Sfax, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5976-2657","authenticated-orcid":false,"given":"Souhir","family":"Guidara","sequence":"additional","affiliation":[{"name":"Department of Genetics, Hedi Chaker Hospital, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ameni","family":"Feki","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Habib Bourguiba Hospital, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hassen","family":"Kamoun","sequence":"additional","affiliation":[{"name":"Department of Genetics, Hedi Chaker Hospital, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Afef","family":"Khanfir","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Habib Bourguiba Hospital, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamel","family":"Daoud","sequence":"additional","affiliation":[{"name":"Department of Radiotherapy, Habib Bourguiba Hospital, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6330-0172","authenticated-orcid":false,"given":"G\u00e9rard-Hubert","family":"Lizard","sequence":"additional","affiliation":[{"name":"Laboratoire Interdisciplinaire Carnot de Bourgogne ICB UMR 6303, University of Bourgogne Europe\/CNRS\/Inserm, 21000 Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5009-9000","authenticated-orcid":false,"given":"Ali","family":"Gargouri","sequence":"additional","affiliation":[{"name":"Center of Biotechnology of Sfax, Laboratory of Molecular Biotechnology of Eukaryotes, University of Sfax, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1319-0061","authenticated-orcid":false,"given":"Raja","family":"Mokdad-Gargouri","sequence":"additional","affiliation":[{"name":"Center of Biotechnology of Sfax, Laboratory of Molecular Biotechnology of Eukaryotes, University of Sfax, Sfax 3018, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"\u0141ukasiewicz, S., Czeczelewski, M., Forma, A., Baj, J., Sitarz, R., and Stanis\u0142awek, A. 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