{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:00:14Z","timestamp":1790866814427,"version":"4.1.0"},"reference-count":64,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background\/Objectives: Annexin A4 (ANXA4) is one of twelve members of the annexin superfamily that bind membrane phospholipids in a calcium-dependent manner, and which are involved in several processes related to the membrane. Methods: To learn more about the role of ANXA4 in cancer, the ANXA4 expression was analyzed by immunohistochemistry (IHC) on tissue microarrays (TMAs) containing 17,654 tumors and precursor lesions from 148 tumor types. Results: ANXA4 immunostaining was found in 10,858 (83.4%) of 13,017 analyzable tumors, and was considered weak in 10.1%, moderate in 16.6%, and strong in 61.8% of cases. Of 148 tumor categories, 144 showed ANXA4 staining in at least one case and 133 included at least one case with strong staining. Among tumor entities with at least 10 evaluable samples, strong ANXA4 staining in &gt;50.0% of cases was seen in 56 of 120 entities, while absence of staining in &gt;50.0% of cases was seen in 13. Clinically important tumor entities where ANXA4 staining was always positive (&gt;99.5%) included gallbladder adenocarcinoma, cholangiocarcinoma, pancreatic\/ampullary, various subtypes of salivary gland tumors, papillary renal cell carcinoma, urothelial carcinoma, squamous and basal cell carcinoma of the skin, and epithelioid mesothelioma. A reduced ANXA4 expression was linked to features of cancer aggressiveness in breast cancer, clear cell renal cell carcinoma, urothelial carcinoma, pancreatic ductal adenocarcinoma, hepatocellular carcinoma, colorectal adenocarcinoma, and follicular thyroid carcinoma. Conclusions: It is concluded that ANXA4 is abundantly expressed in human malignancies and that a reduced expression may be linked to unfavorable disease characteristics in many important tumor entities.<\/jats:p>","DOI":"10.3390\/cancers18193077","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:45:10Z","timestamp":1790865910000},"page":"3077","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression of Annexin A4 in Cancer: A Tissue Microarray Study Involving More than 17,600 Cancers from 148 Tumor Entities"],"prefix":"10.3390","volume":"18","author":[{"given":"Cosima","family":"V\u00f6lkel","sequence":"first","affiliation":[{"name":"Institute of Pathology, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, 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Biosci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1038\/nrm1661","article-title":"Annexins: Linking Ca2+ signalling to membrane dynamics","volume":"6","author":"Gerke","year":"2005","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1152\/physrev.00030.2001","article-title":"Annexins: From structure to function","volume":"82","author":"Gerke","year":"2002","journal-title":"Physiol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"8225","DOI":"10.1021\/bi00193a008","article-title":"Annexin IV reduces the rate of lateral lipid diffusion and changes the fluid phase structure of the lipid bilayer when it binds to negatively charged membranes in the presence of calcium","volume":"33","author":"Gilmanshin","year":"1994","journal-title":"Biochemistry"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3318","DOI":"10.1091\/mbc.e06-01-0041","article-title":"Annexin A4 self-association modulates general membrane protein mobility in living cells","volume":"17","author":"Piljic","year":"2006","journal-title":"Mol. Biol. Cell"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1016\/j.bpj.2013.02.057","article-title":"Quantitative analysis of self-association and mobility of annexin A4 at the plasma membrane","volume":"104","author":"Crosby","year":"2013","journal-title":"Biophys. J."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e71033","DOI":"10.1096\/fj.202501598R","article-title":"ANXA4 Alleviates Cardiomyocyte Injury Associated with Ischemia-Reperfusion by Interfering with NFkappaB p50\u2019s Transcriptional Activation of RAGE","volume":"39","author":"Zhao","year":"2025","journal-title":"FASEB J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3687","DOI":"10.1002\/jcp.30834","article-title":"Evidence of an Annexin A4 mediated plasma membrane repair response to biomechanical strain associated with glaucoma pathogenesis","volume":"237","author":"Vicic","year":"2022","journal-title":"J. Cell Physiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1515\/hsz-2016-0191","article-title":"More than just innate affairs-on the role of annexins in adaptive immunity","volume":"397","author":"Weyd","year":"2016","journal-title":"Biol. Chem."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"112198","DOI":"10.1016\/j.isci.2025.112198","article-title":"Versatile roles of annexin A4 in clear cell renal cell carcinoma: Impact on membrane repair, transcriptional signatures, and composition of the tumor microenvironment","volume":"28","author":"Wess","year":"2025","journal-title":"iScience"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Lin, L.L., Huang, H.C., and Juan, H.F. (2012). Revealing the molecular mechanism of gastric cancer marker annexin A4 in cancer cell proliferation using exon arrays. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0044615"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2102","DOI":"10.3892\/ijmm.2020.4757","article-title":"p53 and ANXA4\/NF-kappaB p50 complexes regulate cell proliferation, apoptosis and tumor progression in ovarian clear cell carcinoma","volume":"46","author":"Liu","year":"2020","journal-title":"Int. J. Mol. Med."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Prieto-Fernandez, L., Menendez, S.T., Otero-Rosales, M., Montoro-Jimenez, I., Hermida-Prado, F., Garcia-Pedrero, J.M., and Alvarez-Teijeiro, S. (2022). Pathobiological functions and clinical implications of annexin dysregulation in human cancers. Front. Cell Dev. Biol., 10.","DOI":"10.3389\/fcell.2022.1009908"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1007\/s004180050275","article-title":"Differential expression of annexins I, II and IV in human tissues: An immunohistochemical study","volume":"110","author":"Dreier","year":"1998","journal-title":"Histochem. Cell Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"C481","DOI":"10.1152\/ajpcell.00404.2018","article-title":"ANXA4 promotes trophoblast invasion via the PI3K\/Akt\/eNOS pathway in preeclampsia","volume":"316","author":"Xu","year":"2019","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1038\/sj.bjc.6604128","article-title":"Characterisation and protein expression profiling of annexins in colorectal cancer","volume":"98","author":"Duncan","year":"2008","journal-title":"Br. J. Cancer"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1016\/j.humpath.2012.08.024","article-title":"Overexpression of annexin A4 is associated with chemoresistance in papillary serous adenocarcinoma of the ovary","volume":"44","author":"Choi","year":"2013","journal-title":"Hum. Pathol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1007\/s00345-010-0575-x","article-title":"The role of annexins I, II and IV in tumor development, progression and metastasis of human penile squamous cell carcinomas","volume":"29","author":"Protzel","year":"2011","journal-title":"World J. Urol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"108093","DOI":"10.18632\/oncotarget.10226","article-title":"Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma","volume":"8","author":"Wang","year":"2017","journal-title":"Oncotarget"},{"key":"ref_20","first-page":"515","article-title":"The Role of Annexin A4 in Triple-Negative Breast Cancer Progression and Its Clinical Application","volume":"46","author":"Zhang","year":"2016","journal-title":"Ann. Clin. Lab. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"31056","DOI":"10.1038\/srep31056","article-title":"Annexin A4-nuclear factor-kappaB feedback circuit regulates cell malignant behavior and tumor growth in gallbladder cancer","volume":"6","author":"Yao","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"29927","DOI":"10.18632\/oncotarget.9179","article-title":"A Fhit-mimetic peptide suppresses annexin A4-mediated chemoresistance to paclitaxel in lung cancer cells","volume":"7","author":"Gaudio","year":"2016","journal-title":"Oncotarget"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Scala, M.C., Di Micco, S., Lanzillotta, D., Musella, S., Di Sarno, V., Parrino, B., Casciofierro, S.M., Bifulco, G., Trapasso, F., and Campiglia, P. (2021). Overcome Chemoresistance: Biophysical and Structural Analysis of Synthetic FHIT-Derived Peptides. Front. Mol. Biosci., 8.","DOI":"10.3389\/fmolb.2021.715263"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.bbrc.2012.03.144","article-title":"Annexin A4 is a possible biomarker for cisplatin susceptibility of malignant mesothelioma cells","volume":"421","author":"Yamashita","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"7776","DOI":"10.18632\/oncotarget.2306","article-title":"Annexin A4 induces platinum resistance in a chloride-and calcium-dependent manner","volume":"5","author":"Morimoto","year":"2014","journal-title":"Oncotarget"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.2147\/OTT.S552516","article-title":"Targeting ANXA4 to Overcome Cisplatin Resistance in Ovarian Cancer: A Bioinformatics and in Vitro Study","volume":"18","author":"Wang","year":"2025","journal-title":"Onco Targets Ther."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1007\/s11418-018-1211-0","article-title":"Toosendanin mediates cisplatin sensitization through targeting Annexin A4\/ATP7A in non-small cell lung cancer cells","volume":"72","author":"Zheng","year":"2018","journal-title":"J. Nat. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"10312","DOI":"10.18632\/aging.202793","article-title":"Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma","volume":"13","author":"Peng","year":"2021","journal-title":"Aging"},{"key":"ref_29","first-page":"1285","article-title":"The transcription factor Ikaros inhibits cell proliferation by downregulating ANXA4 expression in hepatocellular carcinoma","volume":"7","author":"Liu","year":"2017","journal-title":"Am. J. Cancer Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Mogami, T., Yokota, N., Asai-Sato, M., Yamada, R., Koizume, S., Sakuma, Y., Yoshihara, M., Nakamura, Y., Takano, Y., and Hirahara, F. (2013). Annexin A4 is involved in proliferation, chemo-resistance and migration and invasion in ovarian clear cell adenocarcinoma cells. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0080359"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"4247","DOI":"10.1021\/pr800152u","article-title":"Proteome analysis of membrane fractions in colorectal carcinomas by using 2D-DIGE saturation labeling","volume":"7","author":"Alfonso","year":"2008","journal-title":"J. Proteome Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1158\/1055-9965.EPI-10-0097","article-title":"Analytic variability in immunohistochemistry biomarker studies","volume":"19","author":"Anagnostou","year":"2010","journal-title":"Cancer Epidemiol. Biomark. Prev."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1007\/978-1-60761-806-5_4","article-title":"Recipient block TMA technique","volume":"664","author":"Mirlacher","year":"2010","journal-title":"Methods Mol. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/978-1-60761-806-5_2","article-title":"Quality aspects of TMA analysis","volume":"664","author":"Tennstedt","year":"2010","journal-title":"Methods Mol. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Simon, R. (2010). Tissue Microarrays: Methods and Protocols, Springer Science+Business Media.","DOI":"10.1007\/978-1-60761-806-5"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"177","DOI":"10.3233\/CBM-220030","article-title":"PD-L1 expression and CD8 positive lymphocytes in human neoplasms: A tissue microarray study on 11,838 tumor samples","volume":"36","author":"Moller","year":"2023","journal-title":"Cancer Biomark."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1186\/s40364-021-00299-4","article-title":"E-Cadherin expression in human tumors: A tissue microarray study on 10,851 tumors","volume":"9","author":"Burandt","year":"2021","journal-title":"Biomark. Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Tribian, L.S., Lennartz, M., Hoflmayer, D., de Wispelaere, N., Dwertmann Rico, S., von Bargen, C., Kind, S., Reiswich, V., Viehweger, F., and Lutz, F. (2023). Diagnostic Role and Prognostic Impact of PSAP Immunohistochemistry: A Tissue Microarray Study on 31,358 Cancer Tissues. Diagnostics, 13.","DOI":"10.3390\/diagnostics13203242"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Buyucek, S., Moller, K., Viehweger, F., Schlichter, R., Menz, A., Luebke, A.M., Reiswich, V., Kluth, M., Hube-Magg, C., and Hinsch, A. (2026). Glucose-transporter 1 (GLUT1) as a prognostic biomarker: Evidence from 14,966 human tumors across 134 cancer types. BMC Cancer, 26.","DOI":"10.1186\/s12885-025-15527-5"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"10663","DOI":"10.1038\/s41598-026-45409-0","article-title":"Low E-cadherin expression is associated with poor prognosis in pulmonal adenocarcinoma","volume":"16","author":"Gehrisch","year":"2026","journal-title":"Sci. Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1007\/978-1-60761-806-5_12","article-title":"Immunohistochemical analysis of tissue microarrays","volume":"664","author":"Simon","year":"2010","journal-title":"Methods Mol. Biol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.canlet.2003.12.002","article-title":"Increased expression and altered location of annexin IV in renal clear cell carcinoma: A possible role in tumour dissemination","volume":"209","author":"Zimmermann","year":"2004","journal-title":"Cancer Lett."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1007\/s00795-014-0090-z","article-title":"Clinicopathological heterogeneity in ovarian clear cell adenocarcinoma: A study on individual therapy practice","volume":"48","author":"Matsuo","year":"2015","journal-title":"Med. Mol. Morphol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e644","DOI":"10.1097\/CAD.0000000000001216","article-title":"Circular RNA circ_0000592 elevates ANXA4 expression via sponging miR-1179 to facilitate tumor progression in gastric cancer","volume":"33","author":"Ji","year":"2022","journal-title":"Anticancer. Drugs"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"14344","DOI":"10.1038\/s41598-018-32494-z","article-title":"Anxa4 mediated airway progenitor cell migration promotes distal epithelial cell fate specification","volume":"8","author":"Jiang","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2271","DOI":"10.1007\/s00018-010-0331-9","article-title":"Annexin A4 interacts with the NF-kappaB p50 subunit and modulates NF-kappaB transcriptional activity in a Ca2+-dependent manner","volume":"67","author":"Jeon","year":"2010","journal-title":"Cell Mol. Life Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1649","DOI":"10.1089\/dna.2020.5570","article-title":"ANXA4 Activates JAK-STAT3 Signaling by Interacting with ANXA1 in Basal-Like Breast Cancer","volume":"39","author":"Li","year":"2020","journal-title":"DNA Cell Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0002-9440(10)63816-3","article-title":"Dysregulation of the annexin family protein family is associated with prostate cancer progression","volume":"162","author":"Xin","year":"2003","journal-title":"Am. J. Pathol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1021\/pr800890j","article-title":"Analysis of the pancreatic tumor progression by a quantitative proteomic approach and immunhistochemical validation","volume":"8","author":"Sitek","year":"2009","journal-title":"J. Proteome Res."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Zhang, H., Zhang, Z., Guo, T., Chen, G., Liu, G., Song, Q., Li, G., Xu, F., Dong, X., and Yang, F. (2023). Annexin A protein family: Focusing on the occurrence, progression and treatment of cancer. Front. Cell Dev. Biol., 11.","DOI":"10.3389\/fcell.2023.1141331"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.molmed.2019.02.004","article-title":"Annexin-A1\u2014A Blessing or a Curse in Cancer?","volume":"25","author":"Foo","year":"2019","journal-title":"Trends Mol. Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1074\/mcp.M111.011205","article-title":"Quantitative proteomics profiling of murine mammary gland cells unravels impact of annexin-1 on DNA damage response, cell adhesion, and migration","volume":"11","author":"Swa","year":"2012","journal-title":"Mol. Cell Proteom."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"3174","DOI":"10.1038\/onc.2011.28","article-title":"Annexin-1 interacts with NEMO and RIP1 to constitutively activate IKK complex and NF-kappaB: Implication in breast cancer metastasis","volume":"30","author":"Bist","year":"2011","journal-title":"Oncogene"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Xiao, Y., Ouyang, C., Huang, W., Tang, Y., Fu, W., and Cheng, A. (2017). Annexin A1 can inhibit the in vitro invasive ability of nasopharyngeal carcinoma cells possibly through Annexin A1\/S100A9\/Vimentin interaction. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0174383"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"eaal3321","DOI":"10.1126\/science.aal3321","article-title":"A subcellular map of the human proteome","volume":"356","author":"Thul","year":"2017","journal-title":"Science"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s13059-014-0560-6","article-title":"Gateways to the FANTOM5 promoter level mammalian expression atlas","volume":"16","author":"Lizio","year":"2015","journal-title":"Genome Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"D752","DOI":"10.1093\/nar\/gky1099","article-title":"Update of the FANTOM web resource: Expansion to provide additional transcriptome atlases","volume":"47","author":"Lizio","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1038\/ng.2653","article-title":"The Genotype-Tissue Expression (GTEx) project","volume":"45","author":"Consortium","year":"2013","journal-title":"Nat. Genet."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1038\/nmeth.3995","article-title":"A proposal for validation of antibodies","volume":"13","author":"Uhlen","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1943","DOI":"10.1038\/labinvest.3780204","article-title":"Validation of tissue microarray technology in breast carcinoma","volume":"80","author":"Camp","year":"2000","journal-title":"Lab. Investig."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1016\/S0002-9440(10)64075-8","article-title":"Validation of tissue microarrays for immunohistochemical profiling of cancer specimens using the example of human fibroblastic tumors","volume":"158","author":"Hoos","year":"2001","journal-title":"Am. J. Pathol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1097\/00000478-200203000-00004","article-title":"Tissue microarray sampling strategy for prostate cancer biomarker analysis","volume":"26","author":"Rubin","year":"2002","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"117","DOI":"10.5114\/pjp.2026.162567","article-title":"Using large-scale tissue microarray resources for the identification and evaluation of old, new, and novel targets for surgical pathology","volume":"77","author":"Sauter","year":"2026","journal-title":"Pol. J. Pathol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"2249","DOI":"10.1016\/S0002-9440(10)63075-1","article-title":"Tissue microarrays for rapid linking of molecular changes to clinical endpoints","volume":"159","author":"Torhorst","year":"2001","journal-title":"Am. J. Pathol."}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/19\/3077\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:48:13Z","timestamp":1790866093000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/19\/3077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,22]]},"references-count":64,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["cancers18193077"],"URL":"https:\/\/doi.org\/10.3390\/cancers18193077","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,22]]}}}