{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:57:07Z","timestamp":1790744227075,"version":"4.1.0"},"reference-count":55,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Health and Medical Research Council","award":["2022\/GNT2019729"],"award-info":[{"award-number":["2022\/GNT2019729"]}],"id":[{"id":"https:\/\/ror.org\/011kf5r70","id-type":"ROR","asserted-by":"crossref"}]},{"award":["2022\/GNT2019729"],"award-info":[{"award-number":["2022\/GNT2019729"]}],"id":[{"id":"https:\/\/ror.org\/011kf5r70","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Medical Research Future Fund","award":["NCRI000108"],"award-info":[{"award-number":["NCRI000108"]}],"id":[{"id":"https:\/\/ror.org\/0513v5k17","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Medical Research Future Fund","award":["2036564"],"award-info":[{"award-number":["2036564"]}],"id":[{"id":"https:\/\/ror.org\/0513v5k17","id-type":"ROR","asserted-by":"crossref"}]},{"award":["NCRI000108"],"award-info":[{"award-number":["NCRI000108"]}],"id":[{"id":"https:\/\/ror.org\/0513v5k17","id-type":"ROR","asserted-by":"publisher"}]},{"award":["2036564"],"award-info":[{"award-number":["2036564"]}],"id":[{"id":"https:\/\/ror.org\/0513v5k17","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Victorian Government","id":[{"id":"https:\/\/ror.org\/02xvc5r05","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/02xvc5r05","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Isabella and Marcus Foundation","id":[{"id":"https:\/\/ror.org\/014gy9m59","id-type":"ROR","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/014gy9m59","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancers"],"abstract":"<jats:p>Background\/Objectives: Diffuse midline gliomas (DMGs) are among the most aggressive paediatric brain tumours, with the pathognomonic H3K27M mutation present in over 80% of cases. This mutation drives epigenetic dysregulation and transcriptional reprogramming, yet its impact on the tumour antigenic landscape remains poorly understood. Given the low mutational burden of DMG, an expanded search beyond neoantigens to include epigenetically dysregulated tumour-associated antigens (TAAs) is critical for advancing antigen-specific immunotherapies. Methods: We performed immunopeptidomic analysis of two patient-derived H3K27M-mutant DMG cell lines (SU-DIPG13 and BT245) and their isogenic counterparts in which only the mutant H3F3A allele was disrupted by CRISPR editing (H3K27M-KO), leaving wild-type histone H3.3 intact. High-resolution mass spectrometry and bioinformatics were used to define H3K27M-associated changes in the immunopeptidome, and functional T cell assays with HLA-matched healthy donor PBMCs assessed peptide immunogenicity. Results: H3K27M modulated the tumour antigenic landscape in a model-specific manner. Loss of the mutant allele increased HLA-I expression in SU-DIPG13 but not BT245, while immunopeptidomic profiling revealed distinct shifts in tumour-associated peptide presentation in both models, indicating that HLA-I abundance alone does not explain these changes. We identified six peptides derived from SLITRK2, PRAME, XKR5 and CBX2 that elicited T-cell responses in vitro; T cells raised against five of these preferentially recognised the parental H3K27M-mutant DMG cells. Several prioritised peptides were detected only in parental H3K27M-mutant cells, supporting mutation-associated, antigen-selective differences in peptide presentation and tumour antigenicity. Conclusions: This study shows that H3K27M modulates the antigenic landscape in DMG in a model-dependent, antigen-selective manner, uncovering immunogenic tumour-associated peptides that could serve as targets for precision immunotherapy and establishing a foundation for T cell-based therapies in this otherwise treatment-refractory disease.<\/jats:p>","DOI":"10.3390\/cancers18193142","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:57:57Z","timestamp":1790611077000},"page":"3142","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["H3K27M Mutation Modulates the Antigenic Landscape in Diffuse Midline Glioma"],"prefix":"10.3390","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3597-4086","authenticated-orcid":false,"given":"Tima","family":"Shamekhi","sequence":"first","affiliation":[{"name":"Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"},{"name":"Department of Medicine, School of Clinical Sciences, Monash University, Clayton, VIC 3168, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6199-0584","authenticated-orcid":false,"given":"Bijun","family":"Zeng","sequence":"additional","affiliation":[{"name":"Peter MacCallum Cancer Centre, Melbourne, VIC 3000, Australia"},{"name":"Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, VIC 3010, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2124-3074","authenticated-orcid":false,"given":"Claire Xin","family":"Sun","sequence":"additional","affiliation":[{"name":"Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Daniel","sequence":"additional","affiliation":[{"name":"Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7038-1214","authenticated-orcid":false,"given":"Terry C. C.","family":"Lim Kam Sian","sequence":"additional","affiliation":[{"name":"Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"},{"name":"Department of Medicine, School of Clinical Sciences, Monash University, Clayton, VIC 3168, Australia"},{"name":"Monash Proteomics and Metabolomics Platform, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2316-7474","authenticated-orcid":false,"given":"Gabriel","family":"Goncalves","sequence":"additional","affiliation":[{"name":"Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"},{"name":"Department of Medicine, School of Clinical Sciences, Monash University, Clayton, VIC 3168, Australia"},{"name":"Monash Proteomics and Metabolomics Platform, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Huang","sequence":"additional","affiliation":[{"name":"Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"},{"name":"Department of Medicine, School of Clinical Sciences, Monash University, Clayton, VIC 3168, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farnaz","family":"Fahimi","sequence":"additional","affiliation":[{"name":"Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"},{"name":"Department of Medicine, School of Clinical Sciences, Monash University, Clayton, VIC 3168, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nivedhitha","family":"Selvakumar","sequence":"additional","affiliation":[{"name":"Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, VIC 3168, Australia"},{"name":"Monash Proteomics and Metabolomics Platform, 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Metabolomics Platform, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6060-8931","authenticated-orcid":false,"given":"Bettina","family":"Kritzer","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, University Children\u2019s Hospital Zurich, 8008 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8738-1878","authenticated-orcid":false,"given":"Ralf B.","family":"Schittenhelm","sequence":"additional","affiliation":[{"name":"Monash Proteomics and Metabolomics Platform, Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Mazzieri","sequence":"additional","affiliation":[{"name":"Peter MacCallum Cancer Centre, Melbourne, VIC 3000, Australia"},{"name":"Sir Peter 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Adv."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.ccell.2017.08.017","article-title":"Integrated Molecular Meta-Analysis of 1000 Pediatric High-Grade and Diffuse Intrinsic Pontine Glioma","volume":"32","author":"Mackay","year":"2017","journal-title":"Cancer Cell"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S92","DOI":"10.1093\/neuonc\/noad164","article-title":"H3K27M mutant glioma: Disease definition and biological underpinnings","volume":"26","author":"Saratsis","year":"2023","journal-title":"Neuro-Oncology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/nature10833","article-title":"Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma","volume":"482","author":"Schwartzentruber","year":"2012","journal-title":"Nature"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"a019521","DOI":"10.1101\/cshperspect.a019521","article-title":"Histone modifications and cancer","volume":"8","author":"Audia","year":"2016","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1007\/s00401-012-0998-0","article-title":"K27M mutation in histone H3.3 defines clinically and biologically distinct subgroups of pediatric diffuse intrinsic pontine gliomas","volume":"124","author":"Buczkowicz","year":"2012","journal-title":"Acta Neuropathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1038\/s41467-019-09140-x","article-title":"H3K27M induces defective chromatin spread of PRC2-mediated repressive H3K27me2\/me3 and is essential for glioma tumorigenesis","volume":"10","author":"Harutyunyan","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"108390","DOI":"10.1016\/j.celrep.2020.108390","article-title":"H3K27M in Gliomas Causes a One-Step Decrease in H3K27 Methylation and Reduced Spreading within the Constraints of H3K36 Methylation","volume":"33","author":"Harutyunyan","year":"2020","journal-title":"Cell Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1408","DOI":"10.1093\/neuonc\/noac117","article-title":"The intrinsic and microenvironmental features of diffuse midline glioma: Implications for the development of effective immunotherapeutic treatment strategies","volume":"24","author":"Persson","year":"2022","journal-title":"Neuro-Oncology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1126\/science.1232245","article-title":"Inhibition of PRC2 Activity by a Gain-of-Function H3 Mutation Found in Pediatric Glioblastoma","volume":"340","author":"Lewis","year":"2013","journal-title":"Science"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kfoury-Beaumont, N., Prakasam, R., Pondugula, S., Lagas, J.S., Matkovich, S., Gontarz, P., Yang, L., Yano, H., Kim, A.H., and Rubin, J.B. (2022). The H3K27M mutation alters stem cell growth, epigenetic regulation, and differentiation potential. BMC Biol., 20.","DOI":"10.1186\/s12915-022-01324-0"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Chen, Y., Zhao, C., Li, S., Wang, J., and Zhang, H. (2023). Immune Microenvironment and Immunotherapies for Diffuse Intrinsic Pontine Glioma. Cancers, 15.","DOI":"10.3390\/cancers15030602"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Kim, S.K., and Cho, S.W. (2022). The Evasion Mechanisms of Cancer Immunity and Drug Intervention in the Tumor Microenvironment. Front. Pharmacol., 13.","DOI":"10.3389\/fphar.2022.868695"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1093\/neuonc\/noy145","article-title":"Characterization of the immune microenvironment of diffuse intrinsic pontine glioma: Implications for development of immunotherapy","volume":"21","author":"Lieberman","year":"2018","journal-title":"Neuro-Oncology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"103453","DOI":"10.1016\/j.ebiom.2021.103453","article-title":"Current knowledge on the immune microenvironment and emerging immunotherapies in diffuse midline glioma","volume":"69","author":"Price","year":"2021","journal-title":"eBioMedicine"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1881","DOI":"10.1038\/s41588-022-01236-3","article-title":"The landscape of tumor cell states and spatial organization in H3-K27M mutant diffuse midline glioma across age and location","volume":"54","author":"Liu","year":"2022","journal-title":"Nat. Genet."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"101733","DOI":"10.1016\/j.smim.2023.101733","article-title":"Antigen discovery for the development of cancer immunotherapy","volume":"66","author":"Ahn","year":"2023","journal-title":"Semin. Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"D\u2019aMico, S., Tempora, P., Melaiu, O., Lucarini, V., Cifaldi, L., Locatelli, F., and Fruci, D. (2022). Targeting the antigen processing and presentation pathway to overcome resistance to immune checkpoint therapy. Front. Immunol., 13.","DOI":"10.3389\/fimmu.2022.948297"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"6325","DOI":"10.1172\/JCI140378","article-title":"Mass cytometry detects H3.3K27M-specific vaccine responses in diffuse midline glioma","volume":"130","author":"Mueller","year":"2020","journal-title":"J. Clin. Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/j.omto.2023.08.005","article-title":"Endogenous H3.3K27M derived peptide restricted to HLA-A\u221702:01 is insufficient for immune-targeting in diffuse midline glioma","volume":"30","author":"Wang","year":"2023","journal-title":"Mol. Ther.-Oncolytics"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Pandey, K., Wang, S.S., Mifsud, N.A., Faridi, P., Davenport, A.J., Webb, A.I., Sandow, J.J., Ayala, R., Monje, M., and Cross, R.S. (2023). A combined immunopeptidomics, proteomics, and cell surface proteomics approach to identify immunotherapy targets for diffuse intrinsic pontine glioma. Front. Oncol., 13.","DOI":"10.3389\/fonc.2023.1192448"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1093\/neuonc\/noz077","article-title":"Immunotherapy for pediatric brain tumors: Past and present","volume":"21","author":"Foster","year":"2019","journal-title":"Neuro-Oncology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2586","DOI":"10.1038\/s41591-023-02555-6","article-title":"A H3K27M-targeted vaccine in adults with diffuse midline glioma","volume":"29","author":"Grassl","year":"2023","journal-title":"Nat. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"660","DOI":"10.1016\/j.ccell.2023.03.007","article-title":"Generation and multi-dimensional profiling of a childhood cancer cell line atlas defines new therapeutic opportunities","volume":"41","author":"Sun","year":"2023","journal-title":"Cancer Cell"},{"key":"ref_25","unstructured":"Powell, D. (2019). drpowell\/degust 4.1.1 [Software], Zenodo."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Lim Kam Sian, T.C.C., Goncalves, G., Steele, J.R., Shamekhi, T., Bramberger, L., Jin, D., Shahbazy, M., Purcell, A.W., Ramarathinam, S., and Stoychev, S. (2023). SAPrIm, a semi-automated protocol for mid-throughput immunopeptidomics. Front. Immunol., 14.","DOI":"10.3389\/fimmu.2023.1107576"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"W449","DOI":"10.1093\/nar\/gkaa379","article-title":"NetMHCpan-4.1 and NetMHCIIpan-4.0: Improved predictions of MHC antigen presentation by concurrent motif deconvolution and integration of MS MHC eluted ligand data","volume":"48","author":"Reynisson","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate structure prediction of biomolecular interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e002071","DOI":"10.1136\/jitc-2020-002071","article-title":"HLA Ligand Atlas: A benign reference of HLA-presented peptides to improve T-cell-based cancer immunotherapy","volume":"9","author":"Marcu","year":"2021","journal-title":"J. Immunother. Cancer"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1260419","DOI":"10.1126\/science.1260419","article-title":"Proteomics. Tissue-Based Map of the Human Proteome","volume":"347","author":"Fagerberg","year":"2015","journal-title":"Science"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1038\/s41587-020-0439-x","article-title":"The nf-core framework for community-curated bioinformatics pipelines","volume":"38","author":"Ewels","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nmeth.4197","article-title":"Salmon provides fast and bias-aware quantification of transcript expression","volume":"14","author":"Patro","year":"2017","journal-title":"Nat. Methods"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1006\/meth.1999.0844","article-title":"DNA-Based Vaccination against Tumors Expressing the P1A Antigen","volume":"19","author":"Rosato","year":"1999","journal-title":"Methods"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1038\/s41467-019-09234-6","article-title":"Metascape provides a biologist-oriented resource for the analysis of systems-level datasets","volume":"10","author":"Zhou","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Damodharan, S., Lara-Velazquez, M., Williamsen, B.C., Helgager, J., and Dey, M. (2022). Diffuse Intrinsic Pontine Glioma: Molecular Landscape, Evolving Treatment Strategies and Emerging Clinical Trials. J. Pers. Med., 12.","DOI":"10.3390\/jpm12050840"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Mandorino, M., Maitra, A., Armenise, D., Baldelli, O.M., Miciaccia, M., Ferorelli, S., Perrone, M.G., and Scilimati, A. (2024). Pediatric Diffuse Midline Glioma H3K27-Altered: From Developmental Origins to Therapeutic Challenges. Cancers, 16.","DOI":"10.3390\/cancers16101814"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1093\/neuonc\/noab106","article-title":"The 2021 WHO Classification of Tumors of the Central Nervous System: A summary","volume":"23","author":"Louis","year":"2021","journal-title":"Neuro-Oncology"},{"key":"ref_39","first-page":"S50","article-title":"Paediatric type diffuse high grade gliomas in the WHO CNS5 classification: What the pathologist needs to know?","volume":"65","author":"Rao","year":"2022","journal-title":"Indian J. Pathol. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/j.ccell.2019.04.004","article-title":"Pervasive H3K27 Acetylation Leads to ERV Expression and a Therapeutic Vulnerability in H3K27M Gliomas","volume":"35","author":"Krug","year":"2019","journal-title":"Cancer Cell"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.ccell.2019.08.008","article-title":"An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer","volume":"36","author":"Burr","year":"2019","journal-title":"Cancer Cell"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1016\/j.celrep.2017.07.007","article-title":"The Histone Methyltransferase Ezh2 Controls Mechanisms of Adaptive Resistance to Tumor Immunotherapy","volume":"20","author":"Zingg","year":"2017","journal-title":"Cell Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2906","DOI":"10.1158\/2159-8290.CD-21-1492","article-title":"Epigenome programming by H3.3K27M mutation creates a dependence of pediatric glioma on SMARCA4","volume":"12","author":"Mo","year":"2022","journal-title":"Cancer Discov."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"D339","DOI":"10.1093\/nar\/gky1006","article-title":"The Immune Epitope Database (IEDB): 2018 update","volume":"47","author":"Vita","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"9194","DOI":"10.1021\/acs.analchem.0c01545","article-title":"Extending the Comprehensiveness of Immunopeptidome Analyses Using Isobaric Peptide Labeling","volume":"92","author":"Pfammatter","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6075","DOI":"10.1038\/s41467-022-33721-y","article-title":"Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes","volume":"13","author":"Mayer","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3726","DOI":"10.1091\/mbc.e14-06-1109","article-title":"Cbx2 stably associates with mitotic chromosomes via a PRC2- or PRC1-independent mechanism and is needed for recruiting PRC1 complex to mitotic chromosomes","volume":"25","author":"Zhen","year":"2014","journal-title":"Mol. Biol. Cell"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"115659","DOI":"10.1016\/j.bone.2020.115659","article-title":"Biological functions of chromobox (CBX) proteins in stem cell self-renewal, lineage-commitment, cancer and development","volume":"143","author":"Bagheri","year":"2021","journal-title":"Bone"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1631\/jzus.B1400077","article-title":"Epigenetic regulation by polycomb group complexes: Focus on roles of CBX proteins","volume":"15","author":"Ma","year":"2014","journal-title":"J. Zhejiang Univ. B"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.1097\/PAS.0000000000001944","article-title":"PRAME Expression in Cancer. A Systematic Immunohistochemical Study of >5800 Epithelial and Nonepithelial Tumors","volume":"46","author":"Kaczorowski","year":"2022","journal-title":"Am. J. Surg. Pathol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1167\/iovs.64.15.36","article-title":"PRAME Expression: A Target for Cancer Immunotherapy and a Prognostic Factor in Uveal Melanoma","volume":"64","author":"Gelmi","year":"2023","journal-title":"Investig. Opthalmology Vis. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e12770","DOI":"10.1111\/cpr.12770","article-title":"The role of the cancer testis antigen PRAME in tumorigenesis and immunotherapy in human cancer","volume":"53","author":"Xu","year":"2020","journal-title":"Cell Prolif."},{"key":"ref_53","first-page":"A1009","article-title":"959 Safety and anti-tumor activity of TCR-engineered autologous, PRAME-directed T cells across multiple advanced solid cancers at low doses\u2014Clinical update on the ACTengine\u00ae IMA203 trial","volume":"9","author":"Wermke","year":"2021","journal-title":"J. Immunother. Cancer"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1186\/s13045-024-01544-7","article-title":"Understanding the immunosuppressive microenvironment of glioma: Mechanistic insights and clinical perspectives","volume":"17","author":"Lin","year":"2024","journal-title":"J. Hematol. Oncol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e1328340","DOI":"10.1080\/2162402X.2017.1328340","article-title":"K27M-mutant histone-3 as a novel target for glioma immunotherapy","volume":"6","author":"Ochs","year":"2017","journal-title":"OncoImmunology"}],"container-title":["Cancers"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/19\/3142\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:12:49Z","timestamp":1790741569000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2072-6694\/18\/19\/3142"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,28]]},"references-count":55,"journal-issue":{"issue":"19","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["cancers18193142"],"URL":"https:\/\/doi.org\/10.3390\/cancers18193142","relation":{},"ISSN":["2072-6694"],"issn-type":[{"value":"2072-6694","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,28]]}}}