{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:37:43Z","timestamp":1787027863542,"version":"build-2736575974"},"reference-count":70,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T00:00:00Z","timestamp":1609113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Soft tissue sarcomas are a large group of different tumor types both in humans and in animals. Among them, fibrosarcoma is the most frequent malignant mesenchymal tumoral form in cats, representing up to 28% of all cat skin tumors, while human fibrosarcoma, fortunately, only represents 5% of all sarcomas and 0.025% of the world-wide burden of tumors. This low incidence in humans leads to consideration of this group of tumoral diseases as rare, so therapeutic options are few due to the difficulty of starting clinical trials. In this context, the identification of research models for fibrosarcomas could be of great interest to deepen knowledge in this field and recognize new or possible biological pathways involved in tumor progression and metastasis. Angiogenesis is considered a fundamental scattering cause of tumor aggressiveness and progression in all forms of cancer, but only a few research parameters were developed and reported to express them quantitatively and qualitatively. The role in angiogenesis of microenvironmental stromal cells, such as fibroblasts, lymphocytes, mast cells, and macrophages, was largely demonstrated since this topic was first approached, while quantification of new vessels and their blood capacity in tumoral area is a relatively recent approach that could be well developed thanks to expertise in immunohistochemistry and image analysis. In this paper, a crossing study evaluating microvascular density (MVD), endothelial area (EA), and Ki-67 proliferative index was reported for a series of formalin-fixed and paraffin-embedded tissue samples from 99 cat patients, affected by cat post-injection fibrosarcoma, by using a till \u00d7400 magnification light microscopy. We aim to demonstrate that cat pets may be considered a useful animal model for better studying the correspondent human diseases and we report, for the first time to our knowledge, experimental data in terms of correlation among MVD, EA, and Ki-67 strictly involved in aggressiveness and tumoral progression.<\/jats:p>","DOI":"10.3390\/cells10010031","type":"journal-article","created":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T10:33:56Z","timestamp":1609151636000},"page":"31","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Microvascular Density, Endothelial Area, and Ki-67 Proliferative Index Correlate Each Other in Cat Post-Injection Fibrosarcoma"],"prefix":"10.3390","volume":"10","author":[{"given":"Rosa","family":"Patruno","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, Section of Veterinary Pathology and Comparative Oncology, University of Bari \u201cAldo Moro\u201d, Strada p.le per Casamassima, km 3, 70010 Valenzano, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6461-3434","authenticated-orcid":false,"given":"Giuseppe","family":"Passantino","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Section of Veterinary Pathology and Comparative Oncology, University of Bari \u201cAldo Moro\u201d, Strada p.le per Casamassima, km 3, 70010 Valenzano, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5074-4951","authenticated-orcid":false,"given":"Carmelo","family":"Laface","sequence":"additional","affiliation":[{"name":"Interventional and Medical Oncology Unit, IRCCS Istituto Tumori \u201cG. Paolo II\u201d, 70124 Bari, Italy"},{"name":"Department of Biomedical Sciences and Clinical Oncology, University of Bari Aldo Moro, 10124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7964-0753","authenticated-orcid":false,"given":"Antonella","family":"Tinelli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Section of Veterinary Pathology and Comparative Oncology, University of Bari \u201cAldo Moro\u201d, Strada p.le per Casamassima, km 3, 70010 Valenzano, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo","family":"Zito","sequence":"additional","affiliation":[{"name":"Pathology Unit, IRCCS Istituto Tumori \u201cG. Paolo II\u201d, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Ruggieri","sequence":"additional","affiliation":[{"name":"Cardiology Unit, University of Bari \u201cAldo Moro\u201d, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Luposella","sequence":"additional","affiliation":[{"name":"Direction D\u00e9partementale de la Coh\u00e9sion Sociale et de la Protection des Populations des VOSGES (DDCSPP88), 88080 Vittel, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Gadaleta","sequence":"additional","affiliation":[{"name":"Interventional and Medical Oncology Unit, IRCCS Istituto Tumori \u201cG. Paolo II\u201d, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariarita","family":"Laforgia","sequence":"additional","affiliation":[{"name":"Pharmacy Unit, IRCCS Istituto Tumori \u201cG. Paolo II\u201d, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4963-8673","authenticated-orcid":false,"given":"Luca","family":"Lacitignola","sequence":"additional","affiliation":[{"name":"Department of Emergency and Organ Transplantation, University of Bari \u2018Aldo Moro\u2019, Strada p.le per Casamassima, km 3, 70010 Valenzano, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8043-6100","authenticated-orcid":false,"given":"Michele","family":"Ammendola","sequence":"additional","affiliation":[{"name":"Department of Health Science, Digestive Surgery Unit, University \u201cMagna Graecia\u201d Medical School, Viale Europa, Germaneto, 88100 Catanzaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8779-1957","authenticated-orcid":false,"given":"Girolamo","family":"Ranieri","sequence":"additional","affiliation":[{"name":"Interventional and Medical Oncology Unit, IRCCS Istituto Tumori \u201cG. Paolo II\u201d, 70124 Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1434-415X","authenticated-orcid":false,"given":"Nicola","family":"Zizzo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Section of Veterinary Pathology and Comparative Oncology, University of Bari \u201cAldo Moro\u201d, Strada p.le per Casamassima, km 3, 70010 Valenzano, Bari, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1038\/nrc2273","article-title":"Translation of new cancer treatments from pet dogs to humans","volume":"8","author":"Paoloni","year":"2008","journal-title":"Nat. Rev. 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