{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T12:22:06Z","timestamp":1774959726930,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,1,8]],"date-time":"2021-01-08T00:00:00Z","timestamp":1610064000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["IG 2018 #21385"],"award-info":[{"award-number":["IG 2018 #21385"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"publisher","award":["IG 2017 #20052"],"award-info":[{"award-number":["IG 2017 #20052"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004710","name":"Fondazione Umberto Veronesi","doi-asserted-by":"publisher","award":["Post-Doctoral Fellowship 2020"],"award-info":[{"award-number":["Post-Doctoral Fellowship 2020"]}],"id":[{"id":"10.13039\/501100004710","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007479","name":"Fondazione Cassa di Risparmio di Padova e Rovigo","doi-asserted-by":"publisher","award":["grant 17\/03"],"award-info":[{"award-number":["grant 17\/03"]}],"id":[{"id":"10.13039\/100007479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["PRIN 2017 #2017PPS2X4_003"],"award-info":[{"award-number":["PRIN 2017 #2017PPS2X4_003"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Camera di Commercio Venezia","award":["PRIN 2017 #2017PPS2X4_003"],"award-info":[{"award-number":["PRIN 2017 #2017PPS2X4_003"]}]},{"name":"Fondazione Roche","award":["Roche per la Ricerca 2018"],"award-info":[{"award-number":["Roche per la Ricerca 2018"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Plasma exosomal microRNAs (miRNAs) are considered as valid circulating biomarkers for cancer diagnosis and prognosis. Quantitative real-time polymerase chain reaction (qRT-PCR), the most commonly used technique to assess circulating miRNA levels, requires a normalization step involving uniformly expressed endogenous miRNAs. However, there is still no consensus on reference miRNAs for plasma exosomal miRNA abundance normalization. In this study, we identified a panel of miRNAs with stable abundance by analyzing public plasma exosome RNA-seq data and selected miR-486-5p, miR-26a-5p, miR-423-5p and miR191-5p as candidate normalizers. Next, we tested the abundance variation of these miRNAs by qRT-PCR in plasma exosomes of healthy donors and pediatric patients with anaplastic large cell lymphoma, Burkitt lymphoma, Hodgkin lymphoma and mature B-cell acute lymphoblastic leukemia. MiR-486-5p and miR-26a-5p showed the most stable levels, both between healthy controls and patients and among the malignancies analyzed. In light of previous reports on miRNA stability in different exosome isolation methods, our data indicated that miR-26a-5p is a bona fide reference miRNA for qRT-PCR normalization to evaluate miRNA abundance from circulating plasma exosomes in studies of hematological malignancies.<\/jats:p>","DOI":"10.3390\/cells10010101","type":"journal-article","created":{"date-parts":[[2021,1,8]],"date-time":"2021-01-08T08:58:34Z","timestamp":1610096314000},"page":"101","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["MiR-26a-5p as a Reference to Normalize MicroRNA qRT-PCR Levels in Plasma Exosomes of Pediatric Hematological Malignancies"],"prefix":"10.3390","volume":"10","author":[{"given":"Carlotta C.","family":"Damanti","sequence":"first","affiliation":[{"name":"Maternal and Child Health Department, Padova University, 35128 Padova, Italy"},{"name":"Istituto di Ricerca Pediatrica Citt\u00e0 della Speranza, 35127 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6338-7677","authenticated-orcid":false,"given":"Enrico","family":"Gaffo","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Padova University, 35121 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Lovisa","sequence":"additional","affiliation":[{"name":"Maternal and Child Health Department, Padova University, 35128 Padova, Italy"},{"name":"Istituto di Ricerca Pediatrica Citt\u00e0 della Speranza, 35127 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Garbin","sequence":"additional","affiliation":[{"name":"Maternal and Child Health Department, Padova University, 35128 Padova, Italy"},{"name":"Istituto di Ricerca Pediatrica Citt\u00e0 della Speranza, 35127 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3061-9539","authenticated-orcid":false,"given":"Piero","family":"Di Battista","sequence":"additional","affiliation":[{"name":"Maternal and Child Health Department, Padova University, 35128 Padova, Italy"},{"name":"Istituto di Ricerca Pediatrica Citt\u00e0 della Speranza, 35127 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilaria","family":"Gallingani","sequence":"additional","affiliation":[{"name":"Maternal and Child Health Department, Padova University, 35128 Padova, Italy"},{"name":"Istituto di Ricerca Pediatrica Citt\u00e0 della Speranza, 35127 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Tosato","sequence":"additional","affiliation":[{"name":"Maternal and Child Health Department, Padova University, 35128 Padova, Italy"},{"name":"Istituto di Ricerca Pediatrica Citt\u00e0 della Speranza, 35127 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Pillon","sequence":"additional","affiliation":[{"name":"Pediatric Hematology, Oncology and Stem Cell Transplant Division, Padova University Hospital, 35128 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Carraro","sequence":"additional","affiliation":[{"name":"Pediatric Hematology, Oncology and Stem Cell Transplant Division, Padova University Hospital, 35128 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2828-0257","authenticated-orcid":false,"given":"Maurizio","family":"Mascarin","sequence":"additional","affiliation":[{"name":"Pediatric Radiotherapy Unit, Centro di Riferimento Oncologico (CRO) di Aviano, IRCCS, 33081 Aviano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caterina","family":"Elia","sequence":"additional","affiliation":[{"name":"Pediatric Radiotherapy Unit, Centro di Riferimento Oncologico (CRO) di Aviano, IRCCS, 33081 Aviano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Biffi","sequence":"additional","affiliation":[{"name":"Maternal and Child Health Department, Padova University, 35128 Padova, Italy"},{"name":"Istituto di Ricerca Pediatrica Citt\u00e0 della Speranza, 35127 Padova, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8240-3070","authenticated-orcid":false,"given":"Stefania","family":"Bortoluzzi","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, Padova University, 35121 Padova, Italy"},{"name":"CRIBI Interdepartmental Research Center for Innovative Biotechnologies (CRIBI), Padova University, 35131 Padova, 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