{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T22:11:37Z","timestamp":1761948697565,"version":"build-2065373602"},"reference-count":22,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T00:00:00Z","timestamp":1611792000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BBSRC-BB\/P020054\/1"],"award-info":[{"award-number":["BBSRC-BB\/P020054\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>With demand rising, pigs are the world\u2019s leading source of meat protein; however significant economic loss and environmental damage can be incurred if boars used for artificial insemination (AI) are hypoprolific (sub-fertile). Growing evidence suggests that semen analysis is an unreliable tool for diagnosing hypoprolificacy, with litter size and farrowing rate being more applicable. Once such data are available, however, any affected boar will have been in service for some time, with significant financial and environmental losses incurred. Reciprocal translocations (RTs) are the leading cause of porcine hypoprolificacy, reportedly present in 0.47% of AI boars. Traditional standard karyotyping, however, relies on animal specific expertise and does not detect more subtle (cryptic) translocations. Previously, we reported development of a multiple hybridisation fluorescence in situ hybridisation (FISH) strategy; here, we report on its use in 1641 AI boars. A total of 15 different RTs were identified in 69 boars, with four further animals XX\/XY chimeric. Therefore, 4.5% had a chromosome abnormality (4.2% with an RT), a 0.88% incidence. Revisiting cases with both karyotype and FISH information, we reanalysed captured images, asking whether the translocation was detectable by karyotyping alone. The results suggest that chromosome translocations in boars may be significantly under-reported, thereby highlighting the need for pre-emptive screening by this method before a boar enters a breeding programme.<\/jats:p>","DOI":"10.3390\/cells10020250","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T05:23:05Z","timestamp":1611811385000},"page":"250","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Rapid Multi-Hybridisation FISH Screening for Balanced Porcine Reciprocal Translocations Suggests a Much Higher Abnormality Rate Than Previously Appreciated"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4270-970X","authenticated-orcid":false,"given":"Rebecca E","family":"O\u2019Connor","sequence":"first","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury Kent CT2 7NJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas G","family":"Kiazim","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury Kent CT2 7NJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia C","family":"Rathje","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury Kent CT2 7NJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca L","family":"Jennings","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury Kent CT2 7NJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7595-3226","authenticated-orcid":false,"given":"Darren K","family":"Griffin","sequence":"additional","affiliation":[{"name":"School of Biosciences, University of Kent, Canterbury Kent CT2 7NJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,1,28]]},"reference":[{"key":"ref_1","unstructured":"United Nations Food and Agriculture Board (2020, February 25). FAOSTAT. Available online: http:\/\/www.fao.org\/faostat\/en\/#home."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/j.theriogenology.2015.07.009","article-title":"Artificial insemination in pigs today","volume":"85","author":"Knox","year":"2016","journal-title":"Theriogenology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.theriogenology.2009.12.020","article-title":"Reproductive consequences of a reciprocal chromosomal translocation in two Duroc boars used to provide semen for artificial insemination","volume":"74","author":"Sanz","year":"2010","journal-title":"Theriogenology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"779","DOI":"10.2527\/jas.2011-4311","article-title":"Application of computer-assisted semen analysis to explain variations in pig fertility","volume":"90","author":"Broekhuijse","year":"2012","journal-title":"J. Anim. 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Available online: https:\/\/www.thepigsite.com\/articles\/new-economic-study-african-swine-fever-outbreak-in-the-us-could-cost-50-billion."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/2\/250\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:16:26Z","timestamp":1760159786000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/2\/250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,28]]},"references-count":22,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,2]]}},"alternative-id":["cells10020250"],"URL":"https:\/\/doi.org\/10.3390\/cells10020250","relation":{},"ISSN":["2073-4409"],"issn-type":[{"type":"electronic","value":"2073-4409"}],"subject":[],"published":{"date-parts":[[2021,1,28]]}}}