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As such, platelets and leukocytes contribute to mechanisms of thromboinflammation after TBI. While these mechanisms have been investigated in experimental models of contusion brain injury, less is known regarding acute alterations following mild closed head injury. To investigate the role of platelet dynamics and bioenergetics after TBI, we employed two distinct, well-established models of TBI in mice: the controlled cortical impact (CCI) model of contusion brain injury and the closed head injury (CHI) model of mild diffuse brain injury. Hematology parameters, platelet-neutrophil aggregation, and platelet respirometry were assessed acutely after injury. CCI resulted in an early drop in blood leukocyte counts, while CHI increased blood leukocyte counts early after injury. Platelet-neutrophil aggregation was altered acutely after CCI compared to sham. Furthermore, platelet bioenergetic coupling efficiency was transiently reduced at 6 h and increased at 24 h post-CCI. After CHI, oxidative phosphorylation in intact platelets was reduced at 6 h and increased at 24 h compared to sham. Taken together, these data demonstrate that brain trauma initiates alterations in platelet-leukocyte dynamics and platelet metabolism, which may be time- and injury-dependent, providing evidence that platelets carry a peripheral signature of brain injury. The unique trend of platelet bioenergetics after two distinct types of TBI suggests the potential for utilization in prognosis.<\/jats:p>","DOI":"10.3390\/cells10030500","type":"journal-article","created":{"date-parts":[[2021,2,26]],"date-time":"2021-02-26T06:47:20Z","timestamp":1614322040000},"page":"500","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Differential Leukocyte and Platelet Profiles in Distinct Models of Traumatic Brain Injury"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7018-0148","authenticated-orcid":false,"given":"William Brad","family":"Hubbard","sequence":"first","affiliation":[{"name":"Spinal Cord and Brain Injury Research Center (SCoBIRC), University of Kentucky, Lexington, KY 40536, USA"},{"name":"Department of Physiology, University of Kentucky, Lexington, KY 40508, USA"},{"name":"Department of Neuroscience, University of Kentucky, Lexington, KY 40508, USA"},{"name":"Lexington Veterans\u2019 Affairs Healthcare System, Lexington, KY 40502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5139-5861","authenticated-orcid":false,"given":"Meenakshi","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5945-5235","authenticated-orcid":false,"given":"Hemendra","family":"Vekaria","sequence":"additional","affiliation":[{"name":"Spinal Cord and Brain Injury Research Center (SCoBIRC), University of Kentucky, Lexington, KY 40536, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanakanagavalli Shravani","family":"Prakhya","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Smita","family":"Joshi","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, University of Kentucky, Lexington, KY 40536, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn E.","family":"Saatman","sequence":"additional","affiliation":[{"name":"Spinal Cord and Brain Injury Research Center (SCoBIRC), University of Kentucky, Lexington, KY 40536, USA"},{"name":"Department of Physiology, University of Kentucky, Lexington, KY 40508, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sidney W.","family":"Whiteheart","sequence":"additional","affiliation":[{"name":"Lexington Veterans\u2019 Affairs Healthcare System, Lexington, KY 40502, USA"},{"name":"Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7418-4760","authenticated-orcid":false,"given":"Patrick G.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Spinal Cord and Brain Injury Research Center (SCoBIRC), University of Kentucky, Lexington, KY 40536, USA"},{"name":"Department of Neuroscience, University of Kentucky, Lexington, KY 40508, USA"},{"name":"Lexington Veterans\u2019 Affairs Healthcare System, Lexington, KY 40502, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1089\/neu.2018.5663","article-title":"A Mild Traumatic Brain Injury in Mice Produces Lasting Deficits in Brain Metabolism","volume":"35","author":"Lyons","year":"2018","journal-title":"J. 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