{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T03:12:41Z","timestamp":1785640361249,"version":"3.56.0"},"reference-count":68,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T00:00:00Z","timestamp":1614643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["19-34-90151"],"award-info":[{"award-number":["19-34-90151"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Volkswagen Stiftung","award":["90218 and 97750"],"award-info":[{"award-number":["90218 and 97750"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Glia are crucial for the normal development and functioning of the nervous system in many animals. Insects are widely used for studies of glia genetics and physiology. Drosophila melanogaster surface glia (perineurial and subperineurial) form a blood\u2013brain barrier in the central nervous system and blood\u2013nerve barrier in the peripheral nervous system. Under the subperineurial glia layer, in the cortical region of the central nervous system, cortex glia encapsulate neuronal cell bodies, whilst in the peripheral nervous system, wrapping glia ensheath axons of peripheral nerves. Here, we show that the expression of the evolutionarily conserved swiss cheese gene is important in several types of glia. swiss cheese knockdown in subperineurial glia leads to morphological abnormalities of these cells. We found that the number of subperineurial glia nuclei is reduced under swiss cheese knockdown, possibly due to apoptosis. In addition, the downregulation of swiss cheese in wrapping glia causes a loss of its integrity. We reveal transcriptome changes under swiss cheese knockdown in subperineurial glia and in cortex + wrapping glia and show that the downregulation of swiss cheese in these types of glia provokes reactive oxygen species acceleration. These results are accompanied by a decline in animal mobility measured by the negative geotaxis performance assay.<\/jats:p>","DOI":"10.3390\/cells10030529","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T10:36:37Z","timestamp":1614681397000},"page":"529","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Morpho-Functional Consequences of Swiss Cheese Knockdown in Glia of Drosophila melanogaster"],"prefix":"10.3390","volume":"10","author":[{"given":"Elena V.","family":"Ryabova","sequence":"first","affiliation":[{"name":"Molecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P.Konstantinov of NRC \u00abKurchatov Institute\u00bb, 188300 Gatchina, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0620-4747","authenticated-orcid":false,"given":"Pavel A.","family":"Melentev","sequence":"additional","affiliation":[{"name":"Molecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P.Konstantinov of NRC \u00abKurchatov Institute\u00bb, 188300 Gatchina, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Artem E.","family":"Komissarov","sequence":"additional","affiliation":[{"name":"Molecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P.Konstantinov of NRC \u00abKurchatov Institute\u00bb, 188300 Gatchina, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina V.","family":"Surina","sequence":"additional","affiliation":[{"name":"Molecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P.Konstantinov of NRC \u00abKurchatov Institute\u00bb, 188300 Gatchina, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina A.","family":"Ivanova","sequence":"additional","affiliation":[{"name":"Molecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P.Konstantinov of NRC \u00abKurchatov Institute\u00bb, 188300 Gatchina, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1582-2037","authenticated-orcid":false,"given":"Natalia","family":"Matiytsiv","sequence":"additional","affiliation":[{"name":"Department of Genetics and Biotechnology, Faculty of Biology, Ivan Franko Lviv National University, 79005 Lviv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3855-0345","authenticated-orcid":false,"given":"Halyna R.","family":"Shcherbata","sequence":"additional","affiliation":[{"name":"Institute of Cell Biochemistry, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana V.","family":"Sarantseva","sequence":"additional","affiliation":[{"name":"Molecular and Radiation Biophysics Division, Petersburg Nuclear Physics Institute Named by B.P.Konstantinov of NRC \u00abKurchatov Institute\u00bb, 188300 Gatchina, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/S0925-4773(99)00239-7","article-title":"Cloning and Expression of the Murine Sws\/NTE Gene","volume":"90","author":"Moser","year":"2000","journal-title":"Mech. 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