{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T17:50:13Z","timestamp":1773597013029,"version":"3.50.1"},"reference-count":48,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2021,3,27]],"date-time":"2021-03-27T00:00:00Z","timestamp":1616803200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2019R1A2C2004214"],"award-info":[{"award-number":["2019R1A2C2004214"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020R1F1A1049719"],"award-info":[{"award-number":["2020R1F1A1049719"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2020R1A2C1102386"],"award-info":[{"award-number":["2020R1A2C1102386"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Muscle tissue is often removed during hamstring tendon graft preparation for anterior cruciate ligament (ACL) reconstruction. The purpose of the study was to test whether preservation of muscle remnants on a tendon graft is beneficial to the graft healing process following ACL reconstruction. Co-culturing of tendon-derived cells (TDCs) and muscle-derived cells (MDCs) was performed at various ratios, and their potential for cell viability and multilineage differentiation was compared to a single TDC cell group. Ligamentous and chondrogenic differentiation was most enhanced when a small population of MDCs was co-cultured with TDCs (6:2 co-culture group). Cell viability and osteogenic differentiation were proportionally enhanced with increasing MDC population size. MDCs co-cultured with TDCs possess both the ability to enhance cell viability and differentiate into other cell lineages.<\/jats:p>","DOI":"10.3390\/cells10040740","type":"journal-article","created":{"date-parts":[[2021,3,28]],"date-time":"2021-03-28T22:09:01Z","timestamp":1616969341000},"page":"740","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Muscle Cell Preservation on Viability and Differentiation of Hamstring Tendon Graft In Vitro"],"prefix":"10.3390","volume":"10","author":[{"given":"Jin Kyu","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery, Hanyang University Hospital, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3034-5029","authenticated-orcid":false,"given":"Sungsin","family":"Jo","sequence":"additional","affiliation":[{"name":"Institute for Rheumatology Research, Hanyang University, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Lim","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute for Rheumatology Research, Hanyang University, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subin","family":"Weon","sequence":"additional","affiliation":[{"name":"Institute for Rheumatology Research, Hanyang University, Seoul 04763, Korea"},{"name":"Department of Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun-Seob","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Gangnam JS Hospital, Seoul 06259, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Il-Hoon","family":"Sung","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, Hanyang University Hospital, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3542-2276","authenticated-orcid":false,"given":"Tae-Hwan","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute for Rheumatology Research, Hanyang University, Seoul 04763, Korea"},{"name":"Department of Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul 04763, Korea"},{"name":"Department of Rheumatology, Hanyang University Hospital for Rheumatic Diseases, Seoul 04763, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1055\/s-0030-1247719","article-title":"Current trends in anterior cruciate ligament reconstruction","volume":"22","author":"Duquin","year":"2009","journal-title":"J. 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