{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T17:08:51Z","timestamp":1788282531328,"version":"build-2803163510"},"reference-count":60,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T00:00:00Z","timestamp":1619740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hedwig-Stalter-Stiftung","award":["2016"],"award-info":[{"award-number":["2016"]}]},{"name":"Competence Network for Congenital Heart Defects","award":["[Nr. 01GI0601 (2014)]"],"award-info":[{"award-number":["[Nr. 01GI0601 (2014)]"]}]},{"name":"German Centre for Cardiovascular Research (DZHK)","award":["[Nr. 81X2800112 (2015)]"],"award-info":[{"award-number":["[Nr. 81X2800112 (2015)]"]}]},{"name":"F\u00f6rdergemeinschaft Kinderherzen\/Deutsche Herzstiftung","award":["2021"],"award-info":[{"award-number":["2021"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Little is known about abundance level changes of circulating microRNAs (miRNAs) and messenger RNAs (mRNA) in patients with Ebstein\u2019s anomaly (EA). Here, we performed an integrated analysis to identify the differentially abundant miRNAs and mRNA targets and to identify the potential therapeutic targets that might be involved in the mechanisms underlying EA. A large panel of human miRNA and mRNA microarrays were conducted to determine the genome-wide expression profiles in the blood of 16 EA patients and 16 age and gender-matched healthy control volunteers (HVs). Differential abundance level of single miRNA and mRNA was validated by Real-Time quantitative PCR (RT-qPCR). Enrichment analyses of altered miRNA and mRNA abundance levels were identified using bioinformatics tools. Altered miRNA and mRNA abundance levels were observed between EA patients and HVs. Among the deregulated miRNAs and mRNAs, 76 miRNAs (49 lower abundance and 27 higher abundance, fold-change of \u22652) and 29 mRNAs (25 higher abundance and 4 lower abundance, fold-change of \u22651.5) were identified in EA patients compared to HVs. Bioinformatics analysis identified 37 pairs of putative miRNA-mRNA interactions. The majority of the correlations were detected between the lower abundance level of miRNA and higher abundance level of mRNA, except for let-7b-5p, which showed a higher abundance level and their target gene, SCRN3, showed a lower abundance level. Pathway enrichment analysis of the deregulated mRNAs identified 35 significant pathways that are mostly involved in signal transduction and cellular interaction pathways. Our findings provide new insights into a potential molecular biomarker(s) for the EA that may guide the development of novel targeting therapies.<\/jats:p>","DOI":"10.3390\/cells10051066","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T10:53:29Z","timestamp":1619780009000},"page":"1066","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Integrated microRNA and mRNA Expression Profiling Identifies Novel Targets and Networks Associated with Ebstein\u2019s Anomaly"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9048-4958","authenticated-orcid":false,"given":"Masood","family":"Abu-Halima","sequence":"first","affiliation":[{"name":"Institute of Human Genetics, Saarland University, 66421 Homburg, Germany"},{"name":"Department of Pediatric Cardiology, Saarland University Medical Center, 66421 Homburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1957-0658","authenticated-orcid":false,"given":"Viktoria","family":"Wagner","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics, Saarland University, 66421 Homburg, Germany"},{"name":"Center for Clinical Bioinformatics, Saarland University, 66123 Saarbr\u00fccken, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3347-9447","authenticated-orcid":false,"given":"Lea Simone","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics, Saarland University, 66421 Homburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5905-9611","authenticated-orcid":false,"given":"Basim M.","family":"Ayesh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medical Sciences, Alaqsa University, Gaza 4051, Palestine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Abd El-Rahman","sequence":"additional","affiliation":[{"name":"Department of Pediatric Cardiology, Saarland University Medical Center, 66421 Homburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7808-9565","authenticated-orcid":false,"given":"Ulrike","family":"Fischer","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics, Saarland University, 66421 Homburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eckart","family":"Meese","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics, Saarland University, 66421 Homburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hashim","family":"Abdul-Khaliq","sequence":"additional","affiliation":[{"name":"Department of Pediatric Cardiology, Saarland University Medical Center, 66421 Homburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1017\/S1047951116001025","article-title":"The changing epidemiology of Ebstein\u2019s anomaly and its relationship with maternal mental health conditions: A European registry-based study","volume":"27","author":"Boyle","year":"2017","journal-title":"Cardiol. Young"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s11886-020-01412-z","article-title":"Ebstein\u2019s Anomaly of the Tricuspid Valve: An Overview of Pathology and Management","volume":"22","author":"Possner","year":"2020","journal-title":"Curr. Cardiol. Rep."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1017\/S1047951104001106","article-title":"The clinical profile of Ebstein\u2019s malformation as seen from the fetus to the adult in 52 patients","volume":"14","author":"Urroz","year":"2004","journal-title":"Cardiol. Young"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1002\/ajmg.a.33883","article-title":"Epidemiology of Ebstein anomaly: Prevalence and patterns in Texas, 1999\u20132005","volume":"155A","author":"Lupo","year":"2011","journal-title":"Am. J. Med. Genet. A"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1002\/tera.1420500208","article-title":"Ebstein\u2019s malformation of the tricuspid valve: Genetic and environmental factors. The Baltimore-Washington Infant Study Group","volume":"50","author":"Ferencz","year":"1994","journal-title":"Teratology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1161\/CIRCULATIONAHA.106.619338","article-title":"Ebstein\u2019s anomaly","volume":"115","author":"Connolly","year":"2007","journal-title":"Circulation"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mercer, C.L., Andreoletti, G., Carroll, A., Salmon, A.P., Temple, I.K., and Ennis, S. (2017). Familial Ebstein Anomaly: Whole Exome Sequencing Identifies Novel Phenotype Associated With FLNA. Circ. Cardiovasc. Genet., 10.","DOI":"10.1161\/CIRCGENETICS.116.001683"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1159\/000488820","article-title":"Identification of a New Candidate Locus for Ebstein Anomaly in 1p36.2","volume":"9","author":"Restrepo","year":"2018","journal-title":"Mol. Syndromol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"466","DOI":"10.1038\/s41586-020-2797-4","article-title":"Cells of the adult human heart","volume":"588","author":"Litvinukova","year":"2020","journal-title":"Nature"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"793","DOI":"10.3389\/fphys.2020.00793","article-title":"Small Things Matter: Relevance of MicroRNAs in Cardiovascular Disease","volume":"11","author":"Peters","year":"2020","journal-title":"Front. Physiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.cell.2018.03.006","article-title":"Metazoan MicroRNAs","volume":"173","author":"Bartel","year":"2018","journal-title":"Cell"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"402","DOI":"10.3389\/fendo.2018.00402","article-title":"Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation","volume":"9","author":"Hayder","year":"2018","journal-title":"Front. Endocrinol. (Lausanne)"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3353","DOI":"10.1093\/nar\/gkz097","article-title":"An estimate of the total number of true human miRNAs","volume":"47","author":"Alles","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1186\/s12967-018-1429-3","article-title":"Deregulated microRNA and mRNA expression profiles in the peripheral blood of patients with Marfan syndrome","volume":"16","author":"Kahraman","year":"2018","journal-title":"J. Transl. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1055\/s-0037-1604083","article-title":"Characterization of micro-RNA Profile in the Blood of Patients with Marfan\u2019s Syndrome","volume":"66","author":"Ludwig","year":"2018","journal-title":"Thorac. Cardiovasc. Surg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1186\/s12967-017-1255-z","article-title":"Analysis of circulating microRNAs in patients with repaired Tetralogy of Fallot with and without heart failure","volume":"15","author":"Meese","year":"2017","journal-title":"J. Transl. Med."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Abu-Halima, M., Meese, E., Saleh, M.A., Keller, A., Abdul-Khaliq, H., and Raedle-Hurst, T. (2019). Micro-RNA 150-5p predicts overt heart failure in patients with univentricular hearts. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0223606"},{"key":"ref_18","first-page":"619083","article-title":"MicroRNA-29b\/c-3p Indicate Advanced Liver Fibrosis\/Cirrhosis in Univentricular Heart Patients With and Without Fontan Palliation","volume":"7","author":"Meese","year":"2020","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Abu-Halima, M., Oberhoffer, F.S., El Rahman, M.A., Jung, A.M., Zemlin, M., Rohrer, T.R., Kahraman, M., Keller, A., Meese, E., and Abdul-Khaliq, H. (2020). Insights from circulating microRNAs in cardiovascular entities in turner syndrome patients. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0231402"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1186\/s12967-017-1213-9","article-title":"Differential expression of microRNAs following cardiopulmonary bypass in children with congenital heart diseases","volume":"15","author":"Poryo","year":"2017","journal-title":"J. Transl. Med."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Abu-Halima, M., Weidinger, J., Poryo, M., Henn, D., Keller, A., Meese, E., and Abdul-Khaliq, H. (2019). Micro-RNA signatures in monozygotic twins discordant for congenital heart defects. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0226164"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/S0022-5223(19)35302-4","article-title":"A new reconstructive operation for Ebstein\u2019s anomaly of the tricuspid valve","volume":"96","author":"Carpentier","year":"1988","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1093\/ehjci\/jex244","article-title":"Standardization of adult transthoracic echocardiography reporting in agreement with recent chamber quantification, diastolic function, and heart valve disease recommendations: An expert consensus document of the European Association of Cardiovascular Imaging","volume":"18","author":"Galderisi","year":"2017","journal-title":"Eur. Heart J. Cardiovasc. Imaging"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"658","DOI":"10.1016\/0735-1097(96)00202-1","article-title":"Doppler index combining systolic and diastolic myocardial performance: Clinical value in cardiac amyloidosis","volume":"28","author":"Tei","year":"1996","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"W521","DOI":"10.1093\/nar\/gkaa309","article-title":"miEAA 2.0: Integrating multi-species microRNA enrichment analysis and workflow management systems","volume":"48","author":"Kern","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"21809","DOI":"10.1038\/s41598-020-78905-y","article-title":"A multivariable miRNA signature delineates the systemic hemodynamic impact of arteriovenous shunt placement in a pilot study","volume":"10","author":"Henn","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"D377","DOI":"10.1093\/nar\/gks1118","article-title":"PANTHER in 2013: Modeling the evolution of gene function, and other gene attributes, in the context of phylogenetic trees","volume":"41","author":"Mi","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Hamberg, M., Backes, C., Fehlmann, T., Hart, M., Meder, B., Meese, E., and Keller, A. (2016). MiRTargetLink--miRNAs, Genes and Interaction Networks. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17040564"},{"key":"ref_29","first-page":"670","article-title":"BNP and haematological parameters are markers of severity of Ebstein\u2019s anomaly: Correlation with CMR and cardiopulmonary exercise testing","volume":"16","author":"Hosch","year":"2015","journal-title":"Eur. Heart J. Cardiovasc. Imaging"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Sicko, R.J., Browne, M.L., Rigler, S.L., Druschel, C.M., Liu, G., Fan, R., Romitti, P.A., Caggana, M., Kay, D.M., and Brody, L.C. (2016). Genetic Variants in Isolated Ebstein Anomaly Implicated in Myocardial Development Pathways. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0165174"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1586\/14779072.2015.1087849","article-title":"Ebstein anomaly review: What\u2019s now, what\u2019s next?","volume":"13","author":"Dearani","year":"2015","journal-title":"Expert Rev. Cardiovasc. Ther."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.tcm.2008.11.005","article-title":"Cardioprotective signaling by endothelin","volume":"18","author":"Schorlemmer","year":"2008","journal-title":"Trends Cardiovasc. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"205","DOI":"10.3233\/JAD-180054","article-title":"Pyk2 is a Novel Tau Tyrosine Kinase that is Regulated by the Tyrosine Kinase Fyn","volume":"64","author":"Li","year":"2018","journal-title":"J. Alzheimers Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1111\/j.1476-5381.2011.01217.x","article-title":"Role of the endothelin axis and its antagonists in the treatment of cancer","volume":"163","author":"Bagnato","year":"2011","journal-title":"Br. J. Pharmacol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Tripathi, S., Flobak, A., Chawla, K., Baudot, A., Bruland, T., Thommesen, L., Kuiper, M., and Laegreid, A. (2015). The gastrin and cholecystokinin receptors mediated signaling network: A scaffold for data analysis and new hypotheses on regulatory mechanisms. BMC Syst. Biol., 9.","DOI":"10.1186\/s12918-015-0181-z"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.ijcard.2016.02.038","article-title":"Cholecystokinin in plasma predicts cardiovascular mortality in elderly females","volume":"209","author":"Goetze","year":"2016","journal-title":"Int. J. Cardiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.yjmcc.2019.09.001","article-title":"Molecular machinery and interplay of apoptosis and autophagy in coronary heart disease","volume":"136","author":"Dong","year":"2019","journal-title":"J. Mol. Cell Cardiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.pbiomolbio.2012.07.002","article-title":"A potential role for integrin signaling in mechanoelectrical feedback","volume":"110","author":"Dabiri","year":"2012","journal-title":"Prog. Biophys. Mol. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1136\/jmedgenet-2018-105807","article-title":"Clinical spectrum and pleiotropic nature of CDH1 germline mutations","volume":"56","author":"Figueiredo","year":"2019","journal-title":"J. Med. Genet."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1038\/35000034","article-title":"The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells","volume":"2","author":"Batlle","year":"2000","journal-title":"Nat. Cell Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"200","DOI":"10.2174\/1871530317666170818120224","article-title":"Exploring the Cardio-metabolic Relevance of T-cadherin: A Pleiotropic Adiponectin Receptor","volume":"17","author":"Clark","year":"2017","journal-title":"Endocr. Metab. Immune Disord. Drug Targets"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/j.cjca.2018.02.026","article-title":"Heart Failure With Preserved Ejection Fraction in Diabetes: Mechanisms and Management","volume":"34","author":"Meagher","year":"2018","journal-title":"Can. J. Cardiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"116676","DOI":"10.1016\/j.lfs.2019.116676","article-title":"Regulation of Runx2 by MicroRNAs in osteoblast differentiation","volume":"232","author":"Narayanan","year":"2019","journal-title":"Life Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1161\/01.RES.0000257913.42552.23","article-title":"MicroRNAs play an essential role in the development of cardiac hypertrophy","volume":"100","author":"Sayed","year":"2007","journal-title":"Circ. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1002\/ejhf.244","article-title":"MicroRNA signatures differentiate preserved from reduced ejection fraction heart failure","volume":"17","author":"Watson","year":"2015","journal-title":"Eur. J. Heart Fail."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1152\/physrev.00041.2015","article-title":"Non-coding RNAs in Development and Disease: Background, Mechanisms, and Therapeutic Approaches","volume":"96","author":"Beermann","year":"2016","journal-title":"Physiol. Rev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1002\/ejhf.495","article-title":"MicroRNAs in heart failure: From biomarker to target for therapy","volume":"18","author":"Vegter","year":"2016","journal-title":"Eur. J. Heart Fail."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"6699","DOI":"10.1038\/s41598-018-24932-9","article-title":"MicroRNA 199a-5p induces apoptosis by targeting JunB","volume":"8","author":"Yan","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s11886-020-01282-5","article-title":"Mechanisms of Neonatal Heart Regeneration","volume":"22","author":"Cardoso","year":"2020","journal-title":"Curr. Cardiol. Rep."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Braile, M., Marcella, S., Cristinziano, L., Galdiero, M.R., Modestino, L., Ferrara, A.L., Varricchi, G., Marone, G., and Loffredo, S. (2020). VEGF-A in Cardiomyocytes and Heart Diseases. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21155294"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"10735","DOI":"10.1038\/s41598-018-29153-8","article-title":"Circulating microRNAs are associated with Pulmonary Hypertension and Development of Chronic Lung Disease in Congenital Diaphragmatic Hernia","volume":"8","author":"Zhang","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.atherosclerosis.2019.03.016","article-title":"Cardioprotective microRNAs: Lessons from stem cell-derived exosomal microRNAs to treat cardiovascular disease","volume":"285","author":"Moghaddam","year":"2019","journal-title":"Atherosclerosis"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1007\/s00246-015-1325-z","article-title":"MiRNA-145 Regulates the Development of Congenital Heart Disease Through Targeting FXN","volume":"37","author":"Wang","year":"2016","journal-title":"Pediatr. Cardiol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Yan, S., and Jiao, K. (2016). Functions of miRNAs during Mammalian Heart Development. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17050789"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s10528-016-9780-7","article-title":"Genetics of Congenital Heart Disease: Past and Present","volume":"55","author":"Muntean","year":"2017","journal-title":"Biochem. Genet."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1086\/513019","article-title":"Disruption of diacylglycerol kinase delta (DGKD) associated with seizures in humans and mice","volume":"80","author":"Leach","year":"2007","journal-title":"Am. J. Hum. Genet."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1093\/jb\/mvs104","article-title":"Recent progress on type II diacylglycerol kinases: The physiological functions of diacylglycerol kinase delta, eta and kappa and their involvement in disease","volume":"152","author":"Sakai","year":"2012","journal-title":"J. Biochem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1093\/nar\/gkw116","article-title":"Distribution of miRNA expression across human tissues","volume":"44","author":"Ludwig","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"D770","DOI":"10.1093\/nar\/gkx701","article-title":"IMOTA: An interactive multi-omics tissue atlas for the analysis of human miRNA-target interactions","volume":"46","author":"Palmieri","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"5644","DOI":"10.1038\/s41598-020-62534-6","article-title":"The relationship of human tissue microRNAs with those from body fluids","volume":"10","author":"Cui","year":"2020","journal-title":"Sci. Rep."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/5\/1066\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:56:04Z","timestamp":1760162164000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/5\/1066"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,30]]},"references-count":60,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["cells10051066"],"URL":"https:\/\/doi.org\/10.3390\/cells10051066","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,30]]}}}