{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T18:14:58Z","timestamp":1780337698072,"version":"3.54.1"},"reference-count":46,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,8]],"date-time":"2021-05-08T00:00:00Z","timestamp":1620432000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL129192"],"award-info":[{"award-number":["HL129192"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL129192-S1"],"award-info":[{"award-number":["HL129192-S1"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["T32HL105324"],"award-info":[{"award-number":["T32HL105324"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM104357"],"award-info":[{"award-number":["P20GM104357"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P30GM103328"],"award-info":[{"award-number":["P30GM103328"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Eclampsia is diagnosed in pregnant women who develop novel seizures. Our laboratory showed that the reduced uterine perfusion pressure (RUPP) rat model of preeclampsia displays reduced latency to drug-induced seizures. While acid sensing ion channels (ASIC1a and 3) are important for reducing seizure longevity and severity, the role of ASIC2a in mediating seizure sensitivity in pregnancy has not been investigated. We hypothesized that 1) RUPP reduces hippocampal ASIC2a, and 2) pregnant mice with reduced ASIC2a (ASIC2a+\/\u2212) have increased seizure sensitivity. On gestational day 18.5, hippocampi from sham and RUPP C57BL\/6 mice were harvested, and ASIC2a was assessed using Western blot. Pregnant wild-type and ASIC2a+\/\u2212 mice received 40 mg\/kg of pentylenetetrazol (i.p.) and were video recorded for 30 min. Behaviors were scored using a modified Racine scale (0\u20137: 0 = no seizure; 7 = respiratory arrest\/death). Seizure severity was classified as mild (score = 1\u20133) or severe (score = 4\u20137). RUPP mice had reduced hippocampal and placental ASIC2a protein. ASIC2a+\/\u2212 mice had reduced latency to seizures, increased seizure duration, increased severe seizure duration, and higher maximum seizure scores. Reduced hippocampal ASIC2a in RUPP mice and increased seizure activity in pregnant ASIC2a+\/\u2212 mice support the hypothesis that reduced ASIC2a increases seizure sensitivity associated with the RUPP.<\/jats:p>","DOI":"10.3390\/cells10051135","type":"journal-article","created":{"date-parts":[[2021,5,8]],"date-time":"2021-05-08T01:43:12Z","timestamp":1620438192000},"page":"1135","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Acid Sensing Ion Channel 2a Is Reduced in the Reduced Uterine Perfusion Pressure Mouse Model and Increases Seizure Susceptibility in Pregnant Mice"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3078-2096","authenticated-orcid":false,"given":"Maria","family":"Jones-Muhammad","sequence":"first","affiliation":[{"name":"Program in Neuroscience, University of Mississippi Medical Center, Jackson, MS 39216, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingmei","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Mississippi Medical Center, Jackson, MS 39216, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loretta","family":"Cain-Shields","sequence":"additional","affiliation":[{"name":"Department of Data Sciences, University of Mississippi Medical Center, Jackson, MS 39216, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"Shaffery","sequence":"additional","affiliation":[{"name":"Department of Psychiatry, University of Mississippi Medical Center, Jackson, MS 39216, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5626-8872","authenticated-orcid":false,"given":"Junie P.","family":"Warrington","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Mississippi Medical Center, Jackson, MS 39216, USA"},{"name":"Department of Neurobiology and Anatomical Sciences, University of Mississippi Medical Center, Jackson, MS 39216, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,8]]},"reference":[{"key":"ref_1","unstructured":"Fingar, K.R., Mabry-Hernandez, I., Ngo-Metzger, Q., Wolff, T., Steiner, C.A., and Elixhauser, A. 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