{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:00:55Z","timestamp":1772895655016,"version":"3.50.1"},"reference-count":31,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T00:00:00Z","timestamp":1621209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008436","name":"Ernst und Berta Grimmke Stiftung","doi-asserted-by":"publisher","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}],"id":[{"id":"10.13039\/501100008436","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Land Baden-W\u00fcrttemberg, Germany","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]},{"name":"Medical Faculty of the University of Heidelberg, Germany","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]},{"name":"Project no. NVKP_16-1\u20132016-0017 (\u2019National Heart Program\u2019) has been implemented with the support provided from the National Research, Development and Innovation Fund of Hungary, financed under the NVKP_16 funding scheme.","award":["NVKP_16-1\u20132016-0017"],"award-info":[{"award-number":["NVKP_16-1\u20132016-0017"]}]},{"name":"Thematic Excellence Program (2020-4.1.1.-TKP2020) of the Ministry for Innovation and Technology in Hungary, within the framework of the Therapeutic Development and Bioimaging thematic programs of the Semmelweis University","award":["2020-4.1.1.-TKP2020"],"award-info":[{"award-number":["2020-4.1.1.-TKP2020"]}]},{"name":"National Research, Development and Innovation Office (NKFIH) of Hungary","award":["K134939"],"award-info":[{"award-number":["K134939"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>In patients undergoing coronary artery bypass grafting (CABG), ischemia\/reperfusion injury (IRI) is the main contributor to organ dysfunction. Aging-induced vascular damage may be further aggravated during CABG. Favorable effects of conditioned medium (CM) from mesenchymal stem cells (MSCs) have been suggested against IRI. We hypothesized that adding CM to saline protects vascular grafts from IRI in rats. We found that CM contains 28 factors involved in apoptosis, inflammation, and oxidative stress. Thoracic aortic rings from 15-month-old rats were explanted and immediately mounted in organ bath chambers (aged group) or underwent 24 h of cold ischemic preservation in saline-supplemented either with vehicle (aged-IR group) or CM (aged-IR+CM group), prior to mounting. Three-month-old rats were used as referent young animals. Aging was associated with an increase in intima-to-media thickness, an increase in collagen content, higher caspase-12 mRNA levels, and immunoreactivity compared to young rats. Impaired endothelium-dependent vasorelaxation to acetylcholine in the aged-IR group compared to the aged-aorta was improved by CM (aged 61 \u00b1 2% vs. aged-IR 38 \u00b1 2% vs. aged-IR+CM 50 \u00b1 3%, p &lt; 0.05). In the aged-IR group, the already high mRNA levels of caspase-12 were decreased by CM. CM alleviates endothelial dysfunction following IRI in 15-month-old rats. The protective effect may be related to the inhibition of caspase-12 expression.<\/jats:p>","DOI":"10.3390\/cells10051231","type":"journal-article","created":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:06:54Z","timestamp":1621296414000},"page":"1231","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Conditioned Medium from Mesenchymal Stem Cells Alleviates Endothelial Dysfunction of Vascular Grafts Submitted to Ischemia\/Reperfusion Injury in 15-Month-Old Rats"],"prefix":"10.3390","volume":"10","author":[{"given":"Sevil","family":"Korkmaz-Ic\u00f6z","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoxin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiliang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paige","family":"Brlecic","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sivakkanan","family":"Loganathan","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany"},{"name":"Department of Cardiac Surgery, University Hospital Halle (Saale), 06120 Halle, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mih\u00e1ly","family":"Ruppert","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany"},{"name":"Heart and Vascular Center, Semmelweis University, 1122 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex Ali","family":"Sayour","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany"},{"name":"Heart and Vascular Center, Semmelweis University, 1122 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tam\u00e1s","family":"Radovits","sequence":"additional","affiliation":[{"name":"Heart and Vascular Center, Semmelweis University, 1122 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Karck","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e1bor","family":"Szab\u00f3","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, University Hospital Heidelberg, 69120 Heidelberg, Germany"},{"name":"Department of Cardiac Surgery, University Hospital Halle (Saale), 06120 Halle, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1007\/BF01062708","article-title":"Pathobiology and Clinical Impact of Reperfusion Injury","volume":"2","author":"Birnbaum","year":"1995","journal-title":"J. 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