{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:10:23Z","timestamp":1785849023492,"version":"3.56.0"},"reference-count":29,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T00:00:00Z","timestamp":1621814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NS073425, NS116182"],"award-info":[{"award-number":["NS073425, NS116182"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union's Framework Program for Research and Innovation Horizon 2020 (2014-2020)","award":["845336"],"award-info":[{"award-number":["845336"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Oligodendrocyte precursor cells (OPCs) display numerous protrusions that extend into the surrounding parenchyma in the brain. Depending on the preparation of the tissue analyzed, these protrusions are more or less visible. We applied six different fixation methods and compared the effect of prolonged and stronger fixation on fluorescence intensity of platelet-derived growth factor receptor alpha, a surface marker of OPCs. Importantly, the fluorescence signal is mostly lost on protrusions as compared to the cell body, which has to be considered for specific analyses. Additionally, we show numerous contacts established between OPCs and the brain vasculature, which will contribute to the understanding of the interactions between these two elements.<\/jats:p>","DOI":"10.3390\/cells10061302","type":"journal-article","created":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T12:38:01Z","timestamp":1621859881000},"page":"1302","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The Impact of Fixation on the Detection of Oligodendrocyte Precursor Cell Morphology and Vascular Associations"],"prefix":"10.3390","volume":"10","author":[{"given":"Friederike","family":"Pfeiffer","sequence":"first","affiliation":[{"name":"Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT 06269, USA"},{"name":"Department of Neurophysiology, Institue of Physiology, Eberhard Karls University of T\u00fcbingen, 72074 T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amin","family":"Sherafat","sequence":"additional","affiliation":[{"name":"Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT 06269, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7252-0812","authenticated-orcid":false,"given":"Akiko","family":"Nishiyama","sequence":"additional","affiliation":[{"name":"Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT 06269, USA"},{"name":"Institute for Systems Genomics, University of Connecticut, Storrs, CT 06269, USA"},{"name":"The Connecticut Institute for Brain and Cognitive Sciences, University of Connecticut, Storrs, CT 06269, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1242\/dev.047951","article-title":"Age-dependent fate and lineage restriction of single NG2 cells","volume":"138","author":"Zhu","year":"2011","journal-title":"Development"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.brainres.2015.08.013","article-title":"Lineage, fate, and fate potential of NG2-glia","volume":"1638 Pt B","author":"Nishiyama","year":"2016","journal-title":"Brain Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1002\/(SICI)1097-4547(19960201)43:3<299::AID-JNR5>3.0.CO;2-E","article-title":"Co-localization of NG2 proteoglycan and PDGF alpha-receptor on O2A progenitor cells in the developing rat brain","volume":"43","author":"Nishiyama","year":"1996","journal-title":"J. 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