{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T03:02:40Z","timestamp":1790650960716,"version":"4.1.0"},"reference-count":47,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T00:00:00Z","timestamp":1622073600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007434","name":"Suzuken Memorial Foundation","doi-asserted-by":"publisher","award":["TE1"],"award-info":[{"award-number":["TE1"]}],"id":[{"id":"10.13039\/100007434","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K11642"],"award-info":[{"award-number":["17K11642"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K11669"],"award-info":[{"award-number":["17K11669"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20K09904"],"award-info":[{"award-number":["20K09904"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["19H03817"],"award-info":[{"award-number":["19H03817"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20H03888"],"award-info":[{"award-number":["20H03888"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013171","name":"Ryobi Teien Memory Foundation","doi-asserted-by":"publisher","award":["TE, CS, KO"],"award-info":[{"award-number":["TE, CS, KO"]}],"id":[{"id":"10.13039\/501100013171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Extracellular vesicles (EV) heterogeneity is a crucial issue in biology and medicine. In addition, tumor-associated macrophages are key components in cancer microenvironment and immunology. We developed a combination method of size exclusion chromatography and concentration filters (SEC-CF) and aimed to characterize different EV types by their size, cargo types, and functions. A human monocytic leukemia cell line THP-1 was differentiated to CD14-positive macrophage-like cells by stimulation with PMA (phorbol 12-myristate 13-acetate) but not M1 or M2 types. Using the SEC-CF method, the following five EV types were fractionated from the culture supernatant of macrophage-like cells: (i) rare large EVs (500\u20133000 nm) reminiscent of apoptosomes, (ii) EVs (100\u2013500 nm) reminiscent of microvesicles (or microparticles), (iii) EVs (80\u2013300 nm) containing CD9-positive large exosomes (EXO-L), (iv) EVs (20\u2013200 nm) containing unidentified vesicles\/particles, and (v) EVs (10\u201370 nm) containing CD63\/HSP90-positive small exosomes (EXO-S) and particles. For a molecular transfer assay, we developed a THP-1-based stable cell line producing a GFP-fused palmitoylation signal (palmGFP) associated with the membrane. The THP1\/palmGFP cells were differentiated into macrophages producing palmGFP-contained EVs. The macrophage\/palmGFP-secreted EXO-S and EXO-L efficiently transferred the palmGFP to receiver human oral carcinoma cells (HSC-3\/palmTomato), as compared to other EV types. In addition, the macrophage-secreted EXO-S and EXO-L significantly reduced the cell viability (ATP content) in oral carcinoma cells. Taken together, the SEC-CF method is useful for the purification of large and small exosomes with higher molecular transfer activities, enabling efficient molecular delivery to target cells.<\/jats:p>","DOI":"10.3390\/cells10061328","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T11:07:02Z","timestamp":1622113622000},"page":"1328","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Exosome-Based Molecular Transfer Activity of Macrophage-Like Cells Involves Viability of Oral Carcinoma Cells: Size Exclusion Chromatography and Concentration Filter Method"],"prefix":"10.3390","volume":"10","author":[{"given":"Yanyin","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8525, Japan"},{"name":"Department of Dental Anesthesiology and Special Care 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Pharmaceutical Sciences, Okayama 700-8525, Japan"},{"name":"Department of Clinical Engineering, Faculty of Life Sciences, Hiroshima Institute of Technology, Hiroshima 731-5193, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman A.","family":"Taha","sequence":"additional","affiliation":[{"name":"Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8525, Japan"},{"name":"Department of Biochemistry, Ain Shams University Faculty of Science, Cairo 11566, Egypt"},{"name":"Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto 606-8502, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3266-2773","authenticated-orcid":false,"given":"Manh Tien","family":"Tran","sequence":"additional","affiliation":[{"name":"Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8525, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiki","family":"Nara","sequence":"additional","affiliation":[{"name":"Research Program for Undergraduate Students, Okayama University Dental School, Okayama 700-8525, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Penggong","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8525, Japan"},{"name":"O-NECUS Program of Okayama University Dental School, Department of Endodontics, School of Stomatology, China Medical University, Shenyang 110002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Fukuoka","sequence":"additional","affiliation":[{"name":"Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8525, Japan"},{"name":"Department of Orthopaedic Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Miyawaki","sequence":"additional","affiliation":[{"name":"Department of Dental Anesthesiology and Special Care Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8525, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8282-2651","authenticated-orcid":false,"given":"Kuniaki","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8525, 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