{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T11:03:32Z","timestamp":1788001412806,"version":"build-2784847793"},"reference-count":49,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T00:00:00Z","timestamp":1629158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","award":["H2020-SC1-2016-RTD\/731944"],"award-info":[{"award-number":["H2020-SC1-2016-RTD\/731944"]}],"id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["J.0018.18"],"award-info":[{"award-number":["J.0018.18"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["EY 013462"],"award-info":[{"award-number":["EY 013462"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cells"],"abstract":"<jats:p>Saliva secretion requires effective translocation of aquaporin 5 (AQP5) water channel to the salivary glands (SGs) acinar apical membrane. Patients with Sj\u00f6gren\u2019s syndrome (SS) display abnormal AQP5 localization within acinar cells from SGs that correlate with sicca manifestation and glands hypofunction. Several proteins such as Prolactin-inducible protein (PIP) may regulate AQP5 trafficking as observed in lacrimal glands from mice. However, the role of the AQP5-PIP complex remains poorly understood. In the present study, we show that PIP interacts with AQP5 in vitro and in mice as well as in human SGs and that PIP misexpression correlates with an altered AQP5 distribution at the acinar apical membrane in PIP knockout mice and SS hMSG. Furthermore, our data show that the protein-protein interaction involves the AQP5 C-terminus and the N-terminal of PIP (one molecule of PIP per AQP5 tetramer). In conclusion, our findings highlight for the first time the role of PIP as a protein controlling AQP5 localization in human salivary glands but extend beyond due to the PIP-AQP5 interaction described in lung and breast cancers.<\/jats:p>","DOI":"10.3390\/cells10082108","type":"journal-article","created":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T21:27:40Z","timestamp":1629235660000},"page":"2108","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Unraveling Human AQP5-PIP Molecular Interaction and Effect on AQP5 Salivary Glands Localization in SS Patients"],"prefix":"10.3390","volume":"10","author":[{"given":"Clara","family":"Chivasso","sequence":"first","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, 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Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Vanhollebeke","sequence":"additional","affiliation":[{"name":"Laboratory of Neurovascular Signaling, Universit\u00e9 Libre de Bruxelles, 6041 Gosselies, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Delforge","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nargis","family":"Bolaky","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7161-7604","authenticated-orcid":false,"given":"Jason","family":"Perret","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Shahnawaz","family":"Soyfoo","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Erasme Hospital, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9283-6902","authenticated-orcid":false,"given":"Stefania","family":"Moscato","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, 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(2020). Current State of Knowledge on Primary Sj\u00f6gren\u2019s Syndrome, an Autoimmune Exocrinopathy. J. Clin. Med., 9.","DOI":"10.3390\/jcm9072299"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4278","DOI":"10.1002\/anie.200460804","article-title":"Aquaporin Water Channels (Nobel Lecture)","volume":"43","author":"Agre","year":"2004","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"13327","DOI":"10.1073\/pnas.0801466105","article-title":"High-Resolution x-Ray Structure of Human Aquaporin 5","volume":"105","author":"Horsefield","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Hosoi, K., Yao, C., Hasegawa, T., Yoshimura, H., and Akamatsu, T. (2020). Dynamics of Salivary Gland AQP5 under Normal and Pathologic Conditions. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21041182"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Delporte, C. (2009). Aquaporins in Secretory Glands and Their Role in Sj\u00f6gren\u2019s Syndrome. Handb. Exp. Pharmacol., 185\u2013201.","DOI":"10.1007\/978-3-540-79885-9_9"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/S0140-6736(00)04140-4","article-title":"Defective Cellular Trafficking of Lacrimal Gland Aquaporin-5 in Sj\u00f6gren\u2019s Syndrome","volume":"357","author":"Tsubota","year":"2001","journal-title":"Lancet"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1038\/labinvest.3780221","article-title":"Abnormal Distribution of Aquaporin-5 Water Channel Protein in Salivary Glands from Sj\u00f6gren\u2019s Syndrome Patients","volume":"81","author":"Steinfeld","year":"2001","journal-title":"Lab. Investig."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"384","DOI":"10.3109\/14397595.2015.1083146","article-title":"Abnormal Distribution of AQP5 in Labial Salivary Glands Is Associated with Poor Saliva Secretion in Patients with Sj\u00f6gren\u2019s Syndrome Including Neuromyelitis Optica Complicated Patients","volume":"26","author":"Yoshimura","year":"2016","journal-title":"Mod. Rheumatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1080\/02713680802262819","article-title":"Altered Distribution of Aquaporin 5 and Its C-Terminal Binding Protein in the Lacrimal Glands of a Mouse Model for Sj\u00f6gren\u2019s Syndrome","volume":"33","author":"Ohashi","year":"2008","journal-title":"Curr. Eye Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2469","DOI":"10.1002\/pmic.201200230","article-title":"Proteomic Analysis Revealed the Altered Tear Protein Profile in a Rabbit Model of Sj\u00f6gren\u2019s Syndrome-Associated Dry Eye","volume":"13","author":"Zhou","year":"2013","journal-title":"Proteomics"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Gallo, A., Martini, D., Sernissi, F., Giacomelli, C., Pepe, P., Rossi, C., Riveros, P., Mosca, M., Alevizos, I., and Baldini, C. (2013). Gross Cystic Disease Fluid Protein-15(GCDFP-15)\/Prolactin-Inducible Protein (PIP) as Functional Salivary Biomarker for Primary Sj\u00f6gren\u2019s Syndrome. J. Genet. Syndr. Gene Ther., 4.","DOI":"10.4172\/2157-7412.1000140"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s12014-019-9245-1","article-title":"Phenotyping Multiple Subsets in Sj\u00f6gren\u2019s Syndrome: A Salivary Proteomic SWATH-MS Approach towards Precision Medicine","volume":"16","author":"Cecchettini","year":"2019","journal-title":"Clin. Proteom."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/BF01534877","article-title":"The Gene for Prolactin-Inducible Protein (PIP), Uniquely Expressed in Exocrine Organs, Maps to Chromosome 7","volume":"15","author":"Myal","year":"1989","journal-title":"Somat. Cell Mol. Genet."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0003-9969(01)00113-3","article-title":"Identification of Mouse Submaxillary Gland Protein in Mouse Saliva and Its Binding to Mouse Oral Bacteria","volume":"47","author":"Lee","year":"2002","journal-title":"Arch. Oral Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4889","DOI":"10.1021\/pr900686s","article-title":"Identification of Tear Fluid Biomarkers in Dry Eye Syndrome Using ITRAQ Quantitative Proteomics","volume":"8","author":"Zhou","year":"2009","journal-title":"J. Proteome Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.jmb.2008.09.072","article-title":"Crystal Structure of the Novel Complex Formed between Zinc Alpha2-Glycoprotein (ZAG) and Prolactin-Inducible Protein (PIP) from Human Seminal Plasma","volume":"384","author":"Hassan","year":"2008","journal-title":"J. Mol. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"6169","DOI":"10.1021\/bi034038a","article-title":"Structural Study of GCDFP-15\/Gp17 in Disease versus Physiological Conditions Using a Proteomic Approach","volume":"42","author":"Caputo","year":"2003","journal-title":"Biochemistry"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1139\/Y09-077","article-title":"Generation and Initial Characterization of the Prolactin-Inducible Protein (PIP) Null Mouse: Accompanying Global Changes in Gene Expression in the Submandibular Gland","volume":"87","author":"Blanchard","year":"2009","journal-title":"Can. J. Physiol. Pharmacol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1105\/tpc.017194","article-title":"Interactions between Plasma Membrane Aquaporins Modulate Their Water Channel Activity","volume":"16","author":"Fetter","year":"2004","journal-title":"Plant Cell"},{"key":"ref_20","first-page":"24","article-title":"Immortalization of Normal Human Salivary Gland Cells with Duct-, Myoepithelial-, Acinar-, or Squamous Phenotype by Transfection with SV40 Ori- Mutant Deoxyribonucleic Acid","volume":"69","author":"Azuma","year":"1993","journal-title":"Lab. Investig."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1107\/S090744490802948X","article-title":"Affinity Tags Can Reduce Merohedral Twinning of Membrane Protein Crystals","volume":"64","author":"Backmark","year":"2008","journal-title":"Acta Crystallogr. Sect. D Biol. Crystallogr."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.exer.2017.02.012","article-title":"Identification of a Direct Aquaporin-0 Binding Site in the Lens-Specific Cytoskeletal Protein Filensin","volume":"159","author":"Wang","year":"2017","journal-title":"Exp. Eye Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1002\/art.39859","article-title":"2016 American College of Rheumatology\/European League Against Rheumatism Classification Criteria for Primary Sj\u00f6gren\u2019s Syndrome: A Consensus and Data-Driven Methodology Involving Three International Patient Cohorts","volume":"69","author":"Shiboski","year":"2017","journal-title":"Arthritis Rheumatol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An Open-Source Platform for Biological-Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Roche, J.V., Nesverova, V., Olsson, C., Deen, P.M., and T\u00f6rnroth-Horsefield, S. (2019). Structural Insights into AQP2 Targeting to Multivesicular Bodies. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20215351"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1038\/nsmb.2630","article-title":"Allosteric mechanism of water-channel gating by Ca2+-calmodulin","volume":"20","author":"Reichow","year":"2013","journal-title":"Nat. Struct. Mol. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1016\/j.cell.2020.03.037","article-title":"Targeting Aquaporin-4 Subcellular Localization to Treat Central Nervous System Edema","volume":"181","author":"Kitchen","year":"2020","journal-title":"Cell"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1152\/physrev.00011.2011","article-title":"Molecular Mechanism of Pancreatic and Salivary Gland Fluid and HCO3 Secretion","volume":"92","author":"Lee","year":"2012","journal-title":"Physiol. Rev."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"20071","DOI":"10.1074\/jbc.274.29.20071","article-title":"Defective Secretion of Saliva in Transgenic Mice Lacking Aquaporin-5 Water Channels","volume":"274","author":"Ma","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"23413","DOI":"10.1074\/jbc.M008760200","article-title":"Salivary Acinar Cells from Aquaporin 5-Deficient Mice Have Decreased Membrane Water Permeability and Altered Cell Volume Regulation","volume":"276","author":"Krane","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1524","DOI":"10.1016\/j.bbagen.2013.08.007","article-title":"Aquaporins in Salivary Glands and Pancreas","volume":"1840","author":"Delporte","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"16140","DOI":"10.1074\/jbc.272.26.16140","article-title":"Water and Glycerol Permeabilities of Aquaporins 1\u20135 and MIP Determined Quantitatively by Expression of Epitope-Tagged Constructs in Xenopus Oocytes","volume":"272","author":"Yang","year":"1997","journal-title":"J. Biol. Chem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1432-1033.1993.tb18347.x","article-title":"Protein Glycosylation. Structural and Functional Aspects","volume":"218","author":"Lis","year":"1993","journal-title":"Eur. J. Biochem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.jmb.2012.01.027","article-title":"Engineering Aggregation Resistance in IgG by Two Independent Mechanisms: Lessons from Comparison of Pichia Pastoris and Mammalian Cell Expression","volume":"417","author":"Schaefer","year":"2012","journal-title":"J. Mol. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.gene.2013.01.062","article-title":"The Effect of \u03b1-Mating Factor Secretion Signal Mutations on Recombinant Protein Expression in Pichia Pastoris","volume":"519","author":"Stark","year":"2013","journal-title":"Gene"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1002\/(SICI)1097-0215(19980925)78:1<76::AID-IJC13>3.0.CO;2-3","article-title":"Differential Antibody Reactivity and CD4 Binding of the Mammary Tumor Marker Protein GCDFP-15 from Breast Cyst and Its Counterparts from Exocrine Epithelia","volume":"78","author":"Caputo","year":"1998","journal-title":"Int. J. Cancer"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5332","DOI":"10.1021\/bi992398l","article-title":"Mapping the CD4 Binding Domain of Gp17, a Glycoprotein Secreted from Seminal Vesicles and Breast Carcinomas","volume":"39","author":"Basmaciogullari","year":"2000","journal-title":"Biochemistry"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.exer.2011.12.016","article-title":"Influence of sex hormones and genetic predisposition in Sj\u00f6gren\u2019s syndrome: A new clue to the immunopathogenesis of dry eye disease","volume":"96","author":"Mostafa","year":"2012","journal-title":"Exp. Eye Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"G365","DOI":"10.1152\/ajpgi.00437.2011","article-title":"Estrogen prevents increased hepatic aquaporin-9 expression and glycerol uptake during starvation","volume":"302","author":"Lebeck","year":"2012","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Wawrzkiewicz-Ja\u0142owiecka, A., Lalik, A., and Soveral, G. (2021). Recent Update on the Molecular Mechanisms of Gonadal Steroids Action in Adipose Tissue. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22105226"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Jiang, X.X., Fei, X.W., Zhao, L., Ye, X.L., Xin, L.B., Qu, Y., Xu, K.H., Wu, R.J., and Lin, J. (2015). Aquaporin 5 Plays a Role in Estrogen-Induced Ectopic Implantation of Endometrial Stromal Cells in Endometriosis. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0145290"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.neo.2014.04.001","article-title":"Prolactin-Induced Protein Is Required for Cell Cycle Progression in Breast Cancer","volume":"16","author":"Naderi","year":"2014","journal-title":"Neoplasia"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1083\/jcb.200709177","article-title":"Reciprocal Interaction with G-Actin and Tropomyosin Is Essential for Aquaporin-2 Trafficking","volume":"182","author":"Noda","year":"2008","journal-title":"J. Cell Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"639859","DOI":"10.3389\/fonc.2021.639859","article-title":"The Prolactin Inducible Protein Modulates Antitumor Immune Responses and Metastasis in a Mouse Model of Triple Negative Breast Cancer","volume":"11","author":"Edechi","year":"2021","journal-title":"Front. Oncol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"C282","DOI":"10.1152\/ajpcell.00496.2020","article-title":"Aquaporin-5 Regulation of Cell-Cell Adhesion Proteins: An Elusive \u201cTail\u201d Story","volume":"320","author":"Login","year":"2021","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1007\/s12253-019-00646-9","article-title":"Aquaporins 1, 3 and 5 in Different Tumors, Their Expression, Prognosis Value and Role as New Therapeutic Targets","volume":"26","author":"Moosavi","year":"2020","journal-title":"Pathol. Oncol. Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s10911-013-9312-6","article-title":"Aquaporin Water Channels in the Mammary Gland: From Physiology to Pathophysiology and Neoplasia","volume":"19","author":"Mobasheri","year":"2014","journal-title":"J. Mammary Gland Biol. Neoplasia"},{"key":"ref_48","first-page":"2150","article-title":"Prolactin-Induced Protein (PIP)-Characterization and Role in Breast Cancer Progression","volume":"8","author":"Urbaniak","year":"2018","journal-title":"Am. J. Cancer Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1097\/PAI.0b013e3181a8e809","article-title":"GCDFP-15 Positive and TTF-1 Negative Primary Lung Neoplasms: A Tissue Microarray Study of 381 Primary Lung Tumors","volume":"17","author":"Wang","year":"2009","journal-title":"Appl. Immunohistochem. Mol. Morphol."}],"container-title":["Cells"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/8\/2108\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:45:29Z","timestamp":1760165129000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2073-4409\/10\/8\/2108"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,17]]},"references-count":49,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2021,8]]}},"alternative-id":["cells10082108"],"URL":"https:\/\/doi.org\/10.3390\/cells10082108","relation":{},"ISSN":["2073-4409"],"issn-type":[{"value":"2073-4409","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,17]]}}}